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The map label for this gene is mltB [H]

Identifier: 213158038

GI number: 213158038

Start: 2814364

End: 2815365

Strand: Direct

Name: mltB [H]

Synonym: AB57_2749

Alternate gene names: 213158038

Gene position: 2814364-2815365 (Clockwise)

Preceding gene: 213158037

Following gene: 213158039

Centisome position: 69.48

GC content: 39.92

Gene sequence:

>1002_bases
ATGTTGTCTCAATTTGTTAATAAAACTTTAAAAGTGCTTGCCTTGTGTACAGGCCTTATTAGCACAAGTCATTTTGCTCA
GGCCAATGATTTTGTAACTCATCCTAACTATTTAAATTTTAAACAAAAAGCCATGAGCACTTATGGCTTAAGCGGTGAAC
AGGTTGACGCCGCTATGAATGGTGCAAAAAATTTACCAAACATTATAAATATTATGACTCGCCCTGGTGAAAGTAAGCCT
TGGTACGACTATCGCTCTATGTTTTTAGTCGAAGGTACTATTCAACGTGGTGTCCGCTTTAAAAACCAATATGCCGATGC
TCTCAATCGTGCTGAACAACAATTTGGTGTCTCTCAAGCGGTTATTCTAGGCATTTTAGGTGTTGAAACAGGTTATGGAG
CCAATAAAGGCTCGTTTATTACTCGTGATGCACTTGCAACTTTAGCTTTTGGCTACCCACGCCGTGCTGAATATTTTGGC
GATGAGTTAGCTGCTCTCATTGCATGGACTTACAAAGAAGGTTATCCAACCAATAGTATTGTAGGTTCTTATGCTGGTGC
CATTGGCTTCCCGCAGTTTATGCCAAGCAACATTAGTAAGTACGGGGTCGACTTTGATGGTAATGGTCATATCGACCTAC
GAAACTCTGCAGAAGATGCGATTGGTTCAATCGCAAATTACTTAGCAAAACAAGGCTGGCAACGCGATCAACCTATTGGT
TTTATGGCTCGTTACACAGGTTCAAATCCGGAAAGTATCATTGCTAAAGATTTGACCCAGCCCACTCCGTATGGTGCACT
AAAAAATCAAGGGATTTCCCCTTTAAATCCGTTAGTCAAAATTGATGACTTAGATATGGTAAATGTTATTCAATTACAAG
ACTATAACGGTCCAATTTACTATTTAACCTATCCGAACTTTCAAGTAATTACGACTTATAACAAAAGCCGAATGTATGCG
ACTGCCGTATGGTTGTTAGGTACAGAAGTAGCTAGCCGATAG

Upstream 100 bases:

>100_bases
TTTATGAGTTATGGCGGTACCGCTATTATTACTTTAATGGCCACTTTTGGTTTAGTCATGTCTATTCATACACATCGATA
ATCTGGAATTTAAAAAAGAT

Downstream 100 bases:

>100_bases
GCTAGCTTTTTGATGAGTTTATGTAAAAGTCAATAAATATTTTTGTTAAATCGTTCACAATACACACATTTTAAATACCT
TGTTACATATTTGGTTATTT

Product: lytic murein transglycosylase B

Products: Muramic Acid Residue [C]

Alternate protein names: 35 kDa soluble lytic transglycosylase; Murein hydrolase B; Slt35 [H]

Number of amino acids: Translated: 333; Mature: 333

Protein sequence:

>333_residues
MLSQFVNKTLKVLALCTGLISTSHFAQANDFVTHPNYLNFKQKAMSTYGLSGEQVDAAMNGAKNLPNIINIMTRPGESKP
WYDYRSMFLVEGTIQRGVRFKNQYADALNRAEQQFGVSQAVILGILGVETGYGANKGSFITRDALATLAFGYPRRAEYFG
DELAALIAWTYKEGYPTNSIVGSYAGAIGFPQFMPSNISKYGVDFDGNGHIDLRNSAEDAIGSIANYLAKQGWQRDQPIG
FMARYTGSNPESIIAKDLTQPTPYGALKNQGISPLNPLVKIDDLDMVNVIQLQDYNGPIYYLTYPNFQVITTYNKSRMYA
TAVWLLGTEVASR

Sequences:

>Translated_333_residues
MLSQFVNKTLKVLALCTGLISTSHFAQANDFVTHPNYLNFKQKAMSTYGLSGEQVDAAMNGAKNLPNIINIMTRPGESKP
WYDYRSMFLVEGTIQRGVRFKNQYADALNRAEQQFGVSQAVILGILGVETGYGANKGSFITRDALATLAFGYPRRAEYFG
DELAALIAWTYKEGYPTNSIVGSYAGAIGFPQFMPSNISKYGVDFDGNGHIDLRNSAEDAIGSIANYLAKQGWQRDQPIG
FMARYTGSNPESIIAKDLTQPTPYGALKNQGISPLNPLVKIDDLDMVNVIQLQDYNGPIYYLTYPNFQVITTYNKSRMYA
TAVWLLGTEVASR
>Mature_333_residues
MLSQFVNKTLKVLALCTGLISTSHFAQANDFVTHPNYLNFKQKAMSTYGLSGEQVDAAMNGAKNLPNIINIMTRPGESKP
WYDYRSMFLVEGTIQRGVRFKNQYADALNRAEQQFGVSQAVILGILGVETGYGANKGSFITRDALATLAFGYPRRAEYFG
DELAALIAWTYKEGYPTNSIVGSYAGAIGFPQFMPSNISKYGVDFDGNGHIDLRNSAEDAIGSIANYLAKQGWQRDQPIG
FMARYTGSNPESIIAKDLTQPTPYGALKNQGISPLNPLVKIDDLDMVNVIQLQDYNGPIYYLTYPNFQVITTYNKSRMYA
TAVWLLGTEVASR

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG2951

COG function: function code M; Membrane-bound lytic murein transglycosylase B

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor; Periplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789053, Length=312, Percent_Identity=38.4615384615385, Blast_Score=196, Evalue=1e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011757 [H]

Pfam domain/function: NA

EC number: 3.2.1.- [C]

Molecular weight: Translated: 36814; Mature: 36814

Theoretical pI: Translated: 8.28; Mature: 8.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSQFVNKTLKVLALCTGLISTSHFAQANDFVTHPNYLNFKQKAMSTYGLSGEQVDAAMN
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHC
GAKNLPNIINIMTRPGESKPWYDYRSMFLVEGTIQRGVRFKNQYADALNRAEQQFGVSQA
CCCCCCHHHHHHCCCCCCCCCHHHHHEEEEEHHHHHCHHHHHHHHHHHHHHHHHHCCHHH
VILGILGVETGYGANKGSFITRDALATLAFGYPRRAEYFGDELAALIAWTYKEGYPTNSI
HHHHHHHCCCCCCCCCCCEEHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHH
VGSYAGAIGFPQFMPSNISKYGVDFDGNGHIDLRNSAEDAIGSIANYLAKQGWQRDQPIG
HHHHHHHCCCCHHCCCCHHHHCCCCCCCCCEEECCCHHHHHHHHHHHHHHCCCCCCCCCE
FMARYTGSNPESIIAKDLTQPTPYGALKNQGISPLNPLVKIDDLDMVNVIQLQDYNGPIY
EEEEECCCCHHHHHHHHCCCCCCCCHHHCCCCCCCCCCEEECCCCCEEEEEEEECCCCEE
YLTYPNFQVITTYNKSRMYATAVWLLGTEVASR
EEECCCEEEEEEECCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLSQFVNKTLKVLALCTGLISTSHFAQANDFVTHPNYLNFKQKAMSTYGLSGEQVDAAMN
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHC
GAKNLPNIINIMTRPGESKPWYDYRSMFLVEGTIQRGVRFKNQYADALNRAEQQFGVSQA
CCCCCCHHHHHHCCCCCCCCCHHHHHEEEEEHHHHHCHHHHHHHHHHHHHHHHHHCCHHH
VILGILGVETGYGANKGSFITRDALATLAFGYPRRAEYFGDELAALIAWTYKEGYPTNSI
HHHHHHHCCCCCCCCCCCEEHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHH
VGSYAGAIGFPQFMPSNISKYGVDFDGNGHIDLRNSAEDAIGSIANYLAKQGWQRDQPIG
HHHHHHHCCCCHHCCCCHHHHCCCCCCCCCEEECCCHHHHHHHHHHHHHHCCCCCCCCCE
FMARYTGSNPESIIAKDLTQPTPYGALKNQGISPLNPLVKIDDLDMVNVIQLQDYNGPIY
EEEEECCCCHHHHHHHHCCCCCCCCHHHCCCCCCCCCCEEECCCCCEEEEEEEECCCCEE
YLTYPNFQVITTYNKSRMYATAVWLLGTEVASR
EEECCCEEEEEEECCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

General reaction: Cleavage Bond [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7476170; 7789526; 9205837; 9278503; 3553176; 9761817; 10570954; 10545329; 10684641 [H]