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The map label for this gene is queF

Identifier: 213158035

GI number: 213158035

Start: 2811587

End: 2812399

Strand: Direct

Name: queF

Synonym: AB57_2746

Alternate gene names: 213158035

Gene position: 2811587-2812399 (Clockwise)

Preceding gene: 213158034

Following gene: 213158036

Centisome position: 69.41

GC content: 39.36

Gene sequence:

>813_bases
ATGAGTGTCGAACAGTCTTTATTGGGTAAAGAAACCCAATATCCAACTAGCTATCAACCCGATGTGTTATTTCCAATTGC
ACGTGCTCAATCTCGTGAAAAATATGCTCATATCCAAGGTATAACCCAAGGTAAAGATTGGTGGCACGTTTTCGAAATTT
CTTGGTTGAATGCTCACGGTATTCCACAGGTTGCTATCGGCAGAATTACACTGCCAGCTTCTTCACCGAATTTGATTGAA
TCAAAGTCATTAAAACTTTATTTCAATAGTCTTAACTTCACTCAGTTTGACTCTACACAGTCATTCATTGAAACAGTTGA
AAAAGATCTATCTGCTGCGGCAGGTGCAAAAGTTGAATTAACCCTATTTCAAGTAGATGATTTAGAGATTTCTAAACCTC
AAGGGATCTGTATTGATGATCTTATGCCAGAGCGTTTAGAACAACATCCTGATGCTACTCTCTTAAAACTTGATGAGTCA
GGTGAAGAAATCGAAGTTGAATTGTATTCTCATCTTTTGAGAAGTAACTGCCCAGTCACGGGTCAACCGGATTGGGGGAC
AGTTTTTATTCGTTTTAAGGGCAAAAAACCTTGTTATCGTAGTCTTTTAGCCTATATTATTTCGTACCGTCAGCATAACG
GTTTCCACGAACAATGTGTCGAACAAATCTTTGCCGATATTTGGCAAAATTTGCAACCTGAAAAGCTGATGGTTTATGCA
ACTTATACTCGCCGCGGTGGACTTGATATCAATCCTTGCCGAGTATCGGATCTTACATGGATGCCAAAACCGATTCGATT
GGCACGACAATAA

Upstream 100 bases:

>100_bases
ACGTGAACGTTTCGTTCTCTTTAATCATTGTGACATTATGCTGTGCTGTACTTTACGGAATTGCTTATTATTTACTTGCT
CGTGGTTCAGGAATGCGTGA

Downstream 100 bases:

>100_bases
AACGAATAATTTGAGGATACACCATGCCGTTTTTTGGTTTTACCCCTTCTGAAGGCTTATTGAACGATATACAGACTGGA
ATTGCGAATAAAAATTCTAG

Product: 7-cyano-7-deazaguanine reductase

Products: NA

Alternate protein names: 7-cyano-7-carbaguanine reductase; NADPH-dependent nitrile oxidoreductase; PreQ(0) reductase

Number of amino acids: Translated: 270; Mature: 269

Protein sequence:

>270_residues
MSVEQSLLGKETQYPTSYQPDVLFPIARAQSREKYAHIQGITQGKDWWHVFEISWLNAHGIPQVAIGRITLPASSPNLIE
SKSLKLYFNSLNFTQFDSTQSFIETVEKDLSAAAGAKVELTLFQVDDLEISKPQGICIDDLMPERLEQHPDATLLKLDES
GEEIEVELYSHLLRSNCPVTGQPDWGTVFIRFKGKKPCYRSLLAYIISYRQHNGFHEQCVEQIFADIWQNLQPEKLMVYA
TYTRRGGLDINPCRVSDLTWMPKPIRLARQ

Sequences:

>Translated_270_residues
MSVEQSLLGKETQYPTSYQPDVLFPIARAQSREKYAHIQGITQGKDWWHVFEISWLNAHGIPQVAIGRITLPASSPNLIE
SKSLKLYFNSLNFTQFDSTQSFIETVEKDLSAAAGAKVELTLFQVDDLEISKPQGICIDDLMPERLEQHPDATLLKLDES
GEEIEVELYSHLLRSNCPVTGQPDWGTVFIRFKGKKPCYRSLLAYIISYRQHNGFHEQCVEQIFADIWQNLQPEKLMVYA
TYTRRGGLDINPCRVSDLTWMPKPIRLARQ
>Mature_269_residues
SVEQSLLGKETQYPTSYQPDVLFPIARAQSREKYAHIQGITQGKDWWHVFEISWLNAHGIPQVAIGRITLPASSPNLIES
KSLKLYFNSLNFTQFDSTQSFIETVEKDLSAAAGAKVELTLFQVDDLEISKPQGICIDDLMPERLEQHPDATLLKLDESG
EEIEVELYSHLLRSNCPVTGQPDWGTVFIRFKGKKPCYRSLLAYIISYRQHNGFHEQCVEQIFADIWQNLQPEKLMVYAT
YTRRGGLDINPCRVSDLTWMPKPIRLARQ

Specific function: Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1)

COG id: COG0780

COG function: function code R; Enzyme related to GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily

Homologues:

Organism=Escherichia coli, GI1789158, Length=271, Percent_Identity=45.3874538745387, Blast_Score=224, Evalue=6e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEF_ACIB3 (B7GZQ9)

Other databases:

- EMBL:   CP001172
- RefSeq:   YP_002325036.1
- ProteinModelPortal:   B7GZQ9
- SMR:   B7GZQ9
- GeneID:   7060584
- GenomeReviews:   CP001172_GR
- KEGG:   abb:ABBFA_001128
- HOGENOM:   HBG289139
- OMA:   PFRSNFE
- ProtClustDB:   PRK11792
- GO:   GO:0005737
- HAMAP:   MF_00817
- InterPro:   IPR016428
- InterPro:   IPR020602
- PIRSF:   PIRSF004750
- TIGRFAMs:   TIGR03138

Pfam domain/function: PF01227 GTP_cyclohydroI

EC number: =1.7.1.13

Molecular weight: Translated: 30920; Mature: 30788

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVEQSLLGKETQYPTSYQPDVLFPIARAQSREKYAHIQGITQGKDWWHVFEISWLNAHG
CCCCHHHHCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEECCCC
IPQVAIGRITLPASSPNLIESKSLKLYFNSLNFTQFDSTQSFIETVEKDLSAAAGAKVEL
CCCEEEEEEEECCCCCCCCCCCCEEEEEECCCCEECCCHHHHHHHHHHHHHHHCCCEEEE
TLFQVDDLEISKPQGICIDDLMPERLEQHPDATLLKLDESGEEIEVELYSHLLRSNCPVT
EEEEECCCEECCCCCCCHHHCCHHHHHHCCCCEEEEECCCCCEEEHHHHHHHHHCCCCCC
GQPDWGTVFIRFKGKKPCYRSLLAYIISYRQHNGFHEQCVEQIFADIWQNLQPEKLMVYA
CCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEEEE
TYTRRGGLDINPCRVSDLTWMPKPIRLARQ
EEECCCCCCCCCEEECCCCCCCCCHHHCCC
>Mature Secondary Structure 
SVEQSLLGKETQYPTSYQPDVLFPIARAQSREKYAHIQGITQGKDWWHVFEISWLNAHG
CCCHHHHCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEECCCC
IPQVAIGRITLPASSPNLIESKSLKLYFNSLNFTQFDSTQSFIETVEKDLSAAAGAKVEL
CCCEEEEEEEECCCCCCCCCCCCEEEEEECCCCEECCCHHHHHHHHHHHHHHHCCCEEEE
TLFQVDDLEISKPQGICIDDLMPERLEQHPDATLLKLDESGEEIEVELYSHLLRSNCPVT
EEEEECCCEECCCCCCCHHHCCHHHHHHCCCCEEEEECCCCCEEEHHHHHHHHHCCCCCC
GQPDWGTVFIRFKGKKPCYRSLLAYIISYRQHNGFHEQCVEQIFADIWQNLQPEKLMVYA
CCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEEEE
TYTRRGGLDINPCRVSDLTWMPKPIRLARQ
EEECCCCCCCCCEEECCCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA