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The map label for this gene is sir1 [H]

Identifier: 213157863

GI number: 213157863

Start: 3452531

End: 3454174

Strand: Reverse

Name: sir1 [H]

Synonym: AB57_3350

Alternate gene names: 213157863

Gene position: 3454174-3452531 (Counterclockwise)

Preceding gene: 213157864

Following gene: 213157862

Centisome position: 85.28

GC content: 40.09

Gene sequence:

>1644_bases
ATGTATTTATATACCGATTTCGATCAGCAGCTGATTAATCAACGAGTTGCTCAGTTCCGCGATCAGACAGAGCGCTATTT
AGCGGGCAAACTTTCTGAAGATGAATACCGCCCATTACGTTTGCAAAATGGTTTATATGTGCAACGTTATGCACCAATGC
TACGTATTGCTGTGCCGTATGGCTTAATGAACTCAAAACAATTACGTAAAATTGCTGAAGTATCAACTCAATATGACCGT
GGCTATGCACACGTATCTACGCGTCAAAATATTCAGCTAAACTGGCCTGCTCTTGAAGATGTGCCAGAAATTTTAGCTGA
ACTCGCAACTGTACAAATGCATGCCATTCAAACCAGTGGTAACTGTATTCGTAACACGACTACTGACCAGTATGCAGGTG
TAGTTGCAGGTGAAATTGCTGATCCACGCCCAACATGTGAATTGATTCGTCAGTGGAGTACATTCCACCCGGAATTTGCA
TTCTTACCACGTAAATTTAAAATTGCCGTTTCTGCACTTGAAGAAAAAGACCGTGCAGCAACAGCATTCCATGATATTGG
TGTGTATATCGTGCGTAATGAAGCAGGCGAGATGGGCTACAAAATCATGGTGGGTGGTGGTTTGGGCCGTACTCCGATTA
TTGGTAGTGTCATTCGTGAGTTTTTACCACGTGAAGATTTAATTGCTTATTTAGAAGCAGTTTTACGTGTTTATAACTTA
CATGGCCGTCGTGATAACAAGTATAAGGCGCGTATCAAAATCTTGGTTAAAGCATTAACGCCTGAAGTATTTGCACAGAA
AGTTGAAGCTGAATTTGAACACACACGTGAAACATTAAAAATTCAGCCAGAAATCTTGAAAAAATTAGACGAAGAGTTCA
CGCCGTTTGATTATCAAGATTTAGAAGATGAAGACTTTACAGCTTTATTTGCTGAGCATCCAAAATTCAAACAATGGTTC
AATATCAATACGCATGCACATAAAGTAAAGGGCTATCGTATTGTTACGATTTCTTTAAAACGTGCAGGCATCGCACCAGG
CGATATGACCACTGAAGAAATGAACTTCATTGCAGATCTCGCTGATAAATATACGTTTGGTGAACTTCGTACGACTCACG
AACAAAACATTGCTTTAGCAGATGTACCTCAAAAAGATTTGTTCGATGTATGGCAAGCGCTTGAACAGCACAACATGGCT
CGTGCGCATATCGGCTTTATTACCGATATTATTAGCTGCCCGGGTGGTGATTTCTGTTCACTTGCAAATGCGAAATCAAT
ACCTATTGCTGAAGCGATTACACGCCGTTTTGAAGATTTAGATAAAGTTTATGATCTTGGTCATTTAGACTTAAATATTT
CTGGCTGTATGAATGCTTGTGGTCACCACCACGTGGGTAACATCGGTATTTTAGGTGTAGATAAAAAAGGTGCTGAGTTC
TATCAAATTACCTTAGGTGGTAATTCAGACCATGATGCTTCAATTGGTGACATCTTAGGACCATCATTTGCAGCAGAAGC
CGTACCTGACGTCATTGAAGAAGTATTAAATACGTATCTTGATTTACGTATTGAAGGTGAGCGTTTCGTTGATACATATC
GTCGTGTAGGAATTCAACCATTTAAGGAGCGTGCATATGCTTAA

Upstream 100 bases:

>100_bases
TCAATAAAACCGTTATTAAATATGCAAAAAAAGCATTTTATTGATGAATAAAAGCTCGCTATGCTATCCGCCTTAATAAT
GAATCTACTAGCCATACGCC

Downstream 100 bases:

>100_bases
TACAGCTCTACCTGTGCTTTATAAAGATGGCACGATTGCAGATAATACTTATCAAATCATTGCTGAAGATGGTGTGATTC
CTCAAGGTGACGTTGTTTTA

Product: sulfite reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 547; Mature: 547

Protein sequence:

>547_residues
MYLYTDFDQQLINQRVAQFRDQTERYLAGKLSEDEYRPLRLQNGLYVQRYAPMLRIAVPYGLMNSKQLRKIAEVSTQYDR
GYAHVSTRQNIQLNWPALEDVPEILAELATVQMHAIQTSGNCIRNTTTDQYAGVVAGEIADPRPTCELIRQWSTFHPEFA
FLPRKFKIAVSALEEKDRAATAFHDIGVYIVRNEAGEMGYKIMVGGGLGRTPIIGSVIREFLPREDLIAYLEAVLRVYNL
HGRRDNKYKARIKILVKALTPEVFAQKVEAEFEHTRETLKIQPEILKKLDEEFTPFDYQDLEDEDFTALFAEHPKFKQWF
NINTHAHKVKGYRIVTISLKRAGIAPGDMTTEEMNFIADLADKYTFGELRTTHEQNIALADVPQKDLFDVWQALEQHNMA
RAHIGFITDIISCPGGDFCSLANAKSIPIAEAITRRFEDLDKVYDLGHLDLNISGCMNACGHHHVGNIGILGVDKKGAEF
YQITLGGNSDHDASIGDILGPSFAAEAVPDVIEEVLNTYLDLRIEGERFVDTYRRVGIQPFKERAYA

Sequences:

>Translated_547_residues
MYLYTDFDQQLINQRVAQFRDQTERYLAGKLSEDEYRPLRLQNGLYVQRYAPMLRIAVPYGLMNSKQLRKIAEVSTQYDR
GYAHVSTRQNIQLNWPALEDVPEILAELATVQMHAIQTSGNCIRNTTTDQYAGVVAGEIADPRPTCELIRQWSTFHPEFA
FLPRKFKIAVSALEEKDRAATAFHDIGVYIVRNEAGEMGYKIMVGGGLGRTPIIGSVIREFLPREDLIAYLEAVLRVYNL
HGRRDNKYKARIKILVKALTPEVFAQKVEAEFEHTRETLKIQPEILKKLDEEFTPFDYQDLEDEDFTALFAEHPKFKQWF
NINTHAHKVKGYRIVTISLKRAGIAPGDMTTEEMNFIADLADKYTFGELRTTHEQNIALADVPQKDLFDVWQALEQHNMA
RAHIGFITDIISCPGGDFCSLANAKSIPIAEAITRRFEDLDKVYDLGHLDLNISGCMNACGHHHVGNIGILGVDKKGAEF
YQITLGGNSDHDASIGDILGPSFAAEAVPDVIEEVLNTYLDLRIEGERFVDTYRRVGIQPFKERAYA
>Mature_547_residues
MYLYTDFDQQLINQRVAQFRDQTERYLAGKLSEDEYRPLRLQNGLYVQRYAPMLRIAVPYGLMNSKQLRKIAEVSTQYDR
GYAHVSTRQNIQLNWPALEDVPEILAELATVQMHAIQTSGNCIRNTTTDQYAGVVAGEIADPRPTCELIRQWSTFHPEFA
FLPRKFKIAVSALEEKDRAATAFHDIGVYIVRNEAGEMGYKIMVGGGLGRTPIIGSVIREFLPREDLIAYLEAVLRVYNL
HGRRDNKYKARIKILVKALTPEVFAQKVEAEFEHTRETLKIQPEILKKLDEEFTPFDYQDLEDEDFTALFAEHPKFKQWF
NINTHAHKVKGYRIVTISLKRAGIAPGDMTTEEMNFIADLADKYTFGELRTTHEQNIALADVPQKDLFDVWQALEQHNMA
RAHIGFITDIISCPGGDFCSLANAKSIPIAEAITRRFEDLDKVYDLGHLDLNISGCMNACGHHHVGNIGILGVDKKGAEF
YQITLGGNSDHDASIGDILGPSFAAEAVPDVIEEVLNTYLDLRIEGERFVDTYRRVGIQPFKERAYA

Specific function: This Enzyme Catalyzes The 6-Electron Reduction Of Sulfite To Sulfide. This Is One Of Several Activities Required For The Biosynthesis Of L-Cysteine From Sulfate. [C]

COG id: COG0155

COG function: function code P; Sulfite reductase, beta subunit (hemoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

None

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005117
- InterPro:   IPR006067
- InterPro:   IPR006066 [H]

Pfam domain/function: PF01077 NIR_SIR; PF03460 NIR_SIR_ferr [H]

EC number: =1.8.7.1 [H]

Molecular weight: Translated: 62126; Mature: 62126

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYLYTDFDQQLINQRVAQFRDQTERYLAGKLSEDEYRPLRLQNGLYVQRYAPMLRIAVPY
CEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCEEHHHHCCHHEEECCC
GLMNSKQLRKIAEVSTQYDRGYAHVSTRQNIQLNWPALEDVPEILAELATVQMHAIQTSG
CCCCHHHHHHHHHHHHHHCCCEEEECCCCCEEECCCCHHHHHHHHHHHHHHHHHEEECCC
NCIRNTTTDQYAGVVAGEIADPRPTCELIRQWSTFHPEFAFLPRKFKIAVSALEEKDRAA
CEEECCCCHHHHCCEECCCCCCCHHHHHHHHHHHCCCCHHHCCHHHHHHHHHHHHHHHHH
TAFHDIGVYIVRNEAGEMGYKIMVGGGLGRTPIIGSVIREFLPREDLIAYLEAVLRVYNL
HHHHHCCEEEEECCCCCCCEEEEECCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
HGRRDNKYKARIKILVKALTPEVFAQKVEAEFEHTRETLKIQPEILKKLDEEFTPFDYQD
CCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCC
LEDEDFTALFAEHPKFKQWFNINTHAHKVKGYRIVTISLKRAGIAPGDMTTEEMNFIADL
CCCCCHHHHHHCCCCHHHHCCCCCCCEEECCEEEEEEEEEECCCCCCCCCHHHHHHHHHH
ADKYTFGELRTTHEQNIALADVPQKDLFDVWQALEQHNMARAHIGFITDIISCPGGDFCS
HHCCCCHHHHCCCCCCEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCC
LANAKSIPIAEAITRRFEDLDKVYDLGHLDLNISGCMNACGHHHVGNIGILGVDKKGAEF
CCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHCCCCCCCCEEEEEECCCCCEE
YQITLGGNSDHDASIGDILGPSFAAEAVPDVIEEVLNTYLDLRIEGERFVDTYRRVGIQP
EEEEECCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHCEEECCHHHHHHHHHCCCCC
FKERAYA
HHHCCCC
>Mature Secondary Structure
MYLYTDFDQQLINQRVAQFRDQTERYLAGKLSEDEYRPLRLQNGLYVQRYAPMLRIAVPY
CEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCEEHHHHCCHHEEECCC
GLMNSKQLRKIAEVSTQYDRGYAHVSTRQNIQLNWPALEDVPEILAELATVQMHAIQTSG
CCCCHHHHHHHHHHHHHHCCCEEEECCCCCEEECCCCHHHHHHHHHHHHHHHHHEEECCC
NCIRNTTTDQYAGVVAGEIADPRPTCELIRQWSTFHPEFAFLPRKFKIAVSALEEKDRAA
CEEECCCCHHHHCCEECCCCCCCHHHHHHHHHHHCCCCHHHCCHHHHHHHHHHHHHHHHH
TAFHDIGVYIVRNEAGEMGYKIMVGGGLGRTPIIGSVIREFLPREDLIAYLEAVLRVYNL
HHHHHCCEEEEECCCCCCCEEEEECCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
HGRRDNKYKARIKILVKALTPEVFAQKVEAEFEHTRETLKIQPEILKKLDEEFTPFDYQD
CCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCC
LEDEDFTALFAEHPKFKQWFNINTHAHKVKGYRIVTISLKRAGIAPGDMTTEEMNFIADL
CCCCCHHHHHHCCCCHHHHCCCCCCCEEECCEEEEEEEEEECCCCCCCCCHHHHHHHHHH
ADKYTFGELRTTHEQNIALADVPQKDLFDVWQALEQHNMARAHIGFITDIISCPGGDFCS
HHCCCCHHHHCCCCCCEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCC
LANAKSIPIAEAITRRFEDLDKVYDLGHLDLNISGCMNACGHHHVGNIGILGVDKKGAEF
CCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHCCCCCCCCEEEEEECCCCCEE
YQITLGGNSDHDASIGDILGPSFAAEAVPDVIEEVLNTYLDLRIEGERFVDTYRRVGIQP
EEEEECCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHCEEECCHHHHHHHHHCCCCC
FKERAYA
HHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA