Click here to switch to the map view.

The map label for this gene is folD2 [H]

Identifier: 213157614

GI number: 213157614

Start: 3198682

End: 3199530

Strand: Reverse

Name: folD2 [H]

Synonym: AB57_3091

Alternate gene names: 213157614

Gene position: 3199530-3198682 (Counterclockwise)

Preceding gene: 213157615

Following gene: 213157613

Centisome position: 78.99

GC content: 41.11

Gene sequence:

>849_bases
GTGGCATTGGTTTTAGACGGTCGTGCATTAGCAAAGCAAATTGAAGAAAATTTGTTGGTACGTGTTGAAGCTTTAAAAGC
GAAAACAGGTCGTACCCCGATTTTGGCGACTATTTTAGTTGGGGATGATGGTGCATCTGCAACTTATGTACGTATGAAAG
GTAATGCCTGCCGCCGCGTAGGTATGGATTCATTAAAAATTGAATTACCACAAGAAACGACAACAGAACAATTATTAGCT
GAAATCGAAAAGCTTAATGCCAATCCAGATGTTCACGGTATTCTTTTACAGCATCCAGTTCCTGAGCAAATTGATGAGCG
CGCATGTTTTGATGCGATCTCTTTAGCAAAAGATGTAGATGGCGTAACTTGCCTTGGTTTTGGCCGTATGGCAATGGGTG
AAGCTGCATATGGTTCAGCGACTCCTGCTGGCATTATGACTATTCTTAAAGAAAACAATATCGAAATTGCTGGCAAACAC
GCAGTTGTAGTTGGTCGTTCAGCAATTTTAGGTAAACCAATGGCAATGATGTTGTTGCAAGCAAATGCGACAGTAACGAT
TTGTCATTCACGTACACAAAACTTACCTGAGCTTGTGAAACAAGCTGACATTATTGTTGGTGCTGTAGGTAAAGCTGAAC
TTATTCAAAAAGATTGGATTAAACAAGGTGCAGTTGTTGTTGACGCTGGTTTCCATCCACGTGATGGTGGTGGTGTAGGC
GATATTCAATTACAAGGTATTGAAGAAATTGCTTCTGCTTATACACCAGTACCGGGTGGCGTTGGTCCAATGACCATTAC
AACTTTGATTCGTCAAACTGTAGAAGCTGCTGAAAAAGCTTTAGGTTAA

Upstream 100 bases:

>100_bases
TTGAGCGAAGATGCACATAAAAACAGCATCTAGAATAAAATTATCAAAATTATCATGTATTATTAGCACATTAAATTCAT
GTCTACCTTGGAGATGTGCT

Downstream 100 bases:

>100_bases
TTTAATTTATTCACGGCAGCATCCTACCGTTTAAGGAAAATGTTAGGAGCTCGTATGTTACTTTTCTTTCGGGATGAGTA
GCGAATGCTACGCAAGACCA

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase [H]

Number of amino acids: Translated: 282; Mature: 281

Protein sequence:

>282_residues
MALVLDGRALAKQIEENLLVRVEALKAKTGRTPILATILVGDDGASATYVRMKGNACRRVGMDSLKIELPQETTTEQLLA
EIEKLNANPDVHGILLQHPVPEQIDERACFDAISLAKDVDGVTCLGFGRMAMGEAAYGSATPAGIMTILKENNIEIAGKH
AVVVGRSAILGKPMAMMLLQANATVTICHSRTQNLPELVKQADIIVGAVGKAELIQKDWIKQGAVVVDAGFHPRDGGGVG
DIQLQGIEEIASAYTPVPGGVGPMTITTLIRQTVEAAEKALG

Sequences:

>Translated_282_residues
MALVLDGRALAKQIEENLLVRVEALKAKTGRTPILATILVGDDGASATYVRMKGNACRRVGMDSLKIELPQETTTEQLLA
EIEKLNANPDVHGILLQHPVPEQIDERACFDAISLAKDVDGVTCLGFGRMAMGEAAYGSATPAGIMTILKENNIEIAGKH
AVVVGRSAILGKPMAMMLLQANATVTICHSRTQNLPELVKQADIIVGAVGKAELIQKDWIKQGAVVVDAGFHPRDGGGVG
DIQLQGIEEIASAYTPVPGGVGPMTITTLIRQTVEAAEKALG
>Mature_281_residues
ALVLDGRALAKQIEENLLVRVEALKAKTGRTPILATILVGDDGASATYVRMKGNACRRVGMDSLKIELPQETTTEQLLAE
IEKLNANPDVHGILLQHPVPEQIDERACFDAISLAKDVDGVTCLGFGRMAMGEAAYGSATPAGIMTILKENNIEIAGKHA
VVVGRSAILGKPMAMMLLQANATVTICHSRTQNLPELVKQADIIVGAVGKAELIQKDWIKQGAVVVDAGFHPRDGGGVGD
IQLQGIEEIASAYTPVPGGVGPMTITTLIRQTVEAAEKALG

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate [H]

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family [H]

Homologues:

Organism=Homo sapiens, GI222136639, Length=292, Percent_Identity=40.7534246575342, Blast_Score=210, Evalue=1e-54,
Organism=Homo sapiens, GI222418558, Length=296, Percent_Identity=38.5135135135135, Blast_Score=185, Evalue=3e-47,
Organism=Homo sapiens, GI94721354, Length=296, Percent_Identity=40.8783783783784, Blast_Score=184, Evalue=1e-46,
Organism=Homo sapiens, GI36796743, Length=210, Percent_Identity=27.6190476190476, Blast_Score=83, Evalue=4e-16,
Organism=Escherichia coli, GI1786741, Length=277, Percent_Identity=42.2382671480144, Blast_Score=209, Evalue=2e-55,
Organism=Caenorhabditis elegans, GI17568735, Length=257, Percent_Identity=38.5214007782101, Blast_Score=174, Evalue=5e-44,
Organism=Saccharomyces cerevisiae, GI6319558, Length=288, Percent_Identity=40.625, Blast_Score=218, Evalue=9e-58,
Organism=Saccharomyces cerevisiae, GI6321643, Length=296, Percent_Identity=35.8108108108108, Blast_Score=182, Evalue=5e-47,
Organism=Drosophila melanogaster, GI17136816, Length=297, Percent_Identity=42.4242424242424, Blast_Score=210, Evalue=8e-55,
Organism=Drosophila melanogaster, GI17136818, Length=297, Percent_Identity=42.4242424242424, Blast_Score=210, Evalue=8e-55,
Organism=Drosophila melanogaster, GI24645718, Length=291, Percent_Identity=40.893470790378, Blast_Score=209, Evalue=2e-54,
Organism=Drosophila melanogaster, GI17137370, Length=291, Percent_Identity=40.893470790378, Blast_Score=209, Evalue=2e-54,
Organism=Drosophila melanogaster, GI62472483, Length=291, Percent_Identity=40.893470790378, Blast_Score=209, Evalue=2e-54,
Organism=Drosophila melanogaster, GI45551871, Length=291, Percent_Identity=40.893470790378, Blast_Score=209, Evalue=2e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631 [H]

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C [H]

EC number: =1.5.1.5; =3.5.4.9 [H]

Molecular weight: Translated: 29760; Mature: 29629

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS00767 THF_DHG_CYH_2 ; PS00136 SUBTILASE_ASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALVLDGRALAKQIEENLLVRVEALKAKTGRTPILATILVGDDGASATYVRMKGNACRRV
CEEEECCHHHHHHHHHHHHEEEEEHHHCCCCCCEEEEEEEECCCCCEEEEEECCCHHHHC
GMDSLKIELPQETTTEQLLAEIEKLNANPDVHGILLQHPVPEQIDERACFDAISLAKDVD
CCCCEEEECCCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCC
GVTCLGFGRMAMGEAAYGSATPAGIMTILKENNIEIAGKHAVVVGRSAILGKPMAMMLLQ
CEEEEECCCHHHCCCCCCCCCCHHHHHEEECCCEEEECCEEEEEECHHHHCCCHHHHEEE
ANATVTICHSRTQNLPELVKQADIIVGAVGKAELIQKDWIKQGAVVVDAGFHPRDGGGVG
CCCEEEEECCCCCCHHHHHHHHCEEEECCCHHHHHHHHHHHCCCEEEECCCCCCCCCCCC
DIQLQGIEEIASAYTPVPGGVGPMTITTLIRQTVEAAEKALG
CEEECCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ALVLDGRALAKQIEENLLVRVEALKAKTGRTPILATILVGDDGASATYVRMKGNACRRV
EEEECCHHHHHHHHHHHHEEEEEHHHCCCCCCEEEEEEEECCCCCEEEEEECCCHHHHC
GMDSLKIELPQETTTEQLLAEIEKLNANPDVHGILLQHPVPEQIDERACFDAISLAKDVD
CCCCEEEECCCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCC
GVTCLGFGRMAMGEAAYGSATPAGIMTILKENNIEIAGKHAVVVGRSAILGKPMAMMLLQ
CEEEEECCCHHHCCCCCCCCCCHHHHHEEECCCEEEECCEEEEEECHHHHCCCHHHHEEE
ANATVTICHSRTQNLPELVKQADIIVGAVGKAELIQKDWIKQGAVVVDAGFHPRDGGGVG
CCCEEEEECCCCCCHHHHHHHHCEEEECCCHHHHHHHHHHHCCCEEEECCCCCCCCCCCC
DIQLQGIEEIASAYTPVPGGVGPMTITTLIRQTVEAAEKALG
CEEECCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA