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The map label for this gene is brnQ [H]

Identifier: 213157586

GI number: 213157586

Start: 2362418

End: 2363719

Strand: Direct

Name: brnQ [H]

Synonym: AB57_2279

Alternate gene names: 213157586

Gene position: 2362418-2363719 (Clockwise)

Preceding gene: 213157584

Following gene: 213157587

Centisome position: 58.32

GC content: 38.17

Gene sequence:

>1302_bases
ATGCAACAGCTTCGTACCGGAGATATTATTGCCTTAGGTTTTATGACCTTTGCCCTCTTTATTGGGGCTGGTAATATTAT
CTTTCCTCCCATTGTCGCTCAACAAGCTGGTGATCATGTATGGCTAGCTGCCATAGGTTTTTTGATTACTGCCGTTGGCT
TACCTGTAATTACCATTATGGCCCTCTCTCGTATGCAGGGTTCCATCGAAATTATTAGTTCACCGCTAGGTCGTATCGCA
AGTCTGATCTTAACTGTAGTTTGTTATCTATCAGTTGGCCCTTTATTTGCTACACCACGCACCGCAACAGTTTCTTATGA
AATTGGTTTCTCATCTTATTTTGGAACGTCTAGCAGTAGCCTTTTAATTTACAGTGCAATTTACTTCGCATTTGTAACTG
TCGTTTCTTTATATCCGAACAAGCTTTTAGACACAGTTGGACATGTTCTAGCTCCTCTGAAAATTATCGCTTTAGCAATT
TTAGGTATTGCAGCGGTTATGATTCCTGCGGGTTATGTTCCTGCTCCAATTAATCATTACGTTAACTCTCCTGTTTCAGA
AGGTTTCGTAAATGGTTATCTCACCATGGATACTTTAGGTGCCCTAGTATTTGGTATCGTCATTATTCAAGCGATCTATT
CGCGTGGTGTTACTGATAACAAACTAGTGACCAAGTATGCAATTATTGCCAGCCTTATTTCTGGTGTAGGACTCACTTTA
GTTTACTTAAGCTTGTTTAAATTAGGTTTGGGTAGCCATGAAGTCGCTCCTAATGCAGCAAATGGTGCTGTAATCCTACA
TGCATATGTTCAACATGCTTTTGGAAATATGGGTTCACTCTTCTTAACAGGTCTCATTTTCTTAGCATGTATGGTTACTG
CAATTGGTTTAACTTGTGCATGTGCTGAATATTTCGCTCAACTTACTAAGTTGCCATATAAGCTTTTAGTTTTCATCTTA
GTTGGTTTTTCTTTCATTATTTCCAACCTTGGATTGACTAAGCTAATTGCCGTATCAGTACCAGTTTTAAGTGCAATTTA
TCCACCTGCAATTGTAGTTATTATGCTCAGTTTCTTCTGGAAATTTTGGCATAAACCATCTTTTATTGTCGGTTCAGTAA
CAAGCATTGCTTTAATTTTCGGAATTATTGACGGGCTTAAGGTTGCAGGTTTTGGTGATTACCTCCCTAATTTCCTTCAA
CACTTACCGTTAAATGAACAAAACTTGGCATGGTTGATCCCATCTTTAGTTGTTCTTGTAGTGACTACTCTTATCGACAA
AGTTAAACATAAAAGCATTTAA

Upstream 100 bases:

>100_bases
ATGATTGATACGGCCTAAAAAAAAGCTTTTTCACATAAAATAGCGCTTGATCATGATCTCTTTTGCTCTAAAATTCTTTC
TTTTTTGTTAAATTTAATTT

Downstream 100 bases:

>100_bases
TCTGATGTTTTTTTGAAATCGAGTTTTACCCAAACATGATTTTATATATCGAATAAAATGCTGCGTTTACCCAACCGGCA
TTTTATTTCTAACATCAGCA

Product: branched-chain amino acid transport system II carrier protein

Products: L-leucine [Cytoplasm]; L-valine [Cytoplasm]; Na (I) [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: LIV-III [H]

Number of amino acids: Translated: 433; Mature: 433

Protein sequence:

>433_residues
MQQLRTGDIIALGFMTFALFIGAGNIIFPPIVAQQAGDHVWLAAIGFLITAVGLPVITIMALSRMQGSIEIISSPLGRIA
SLILTVVCYLSVGPLFATPRTATVSYEIGFSSYFGTSSSSLLIYSAIYFAFVTVVSLYPNKLLDTVGHVLAPLKIIALAI
LGIAAVMIPAGYVPAPINHYVNSPVSEGFVNGYLTMDTLGALVFGIVIIQAIYSRGVTDNKLVTKYAIIASLISGVGLTL
VYLSLFKLGLGSHEVAPNAANGAVILHAYVQHAFGNMGSLFLTGLIFLACMVTAIGLTCACAEYFAQLTKLPYKLLVFIL
VGFSFIISNLGLTKLIAVSVPVLSAIYPPAIVVIMLSFFWKFWHKPSFIVGSVTSIALIFGIIDGLKVAGFGDYLPNFLQ
HLPLNEQNLAWLIPSLVVLVVTTLIDKVKHKSI

Sequences:

>Translated_433_residues
MQQLRTGDIIALGFMTFALFIGAGNIIFPPIVAQQAGDHVWLAAIGFLITAVGLPVITIMALSRMQGSIEIISSPLGRIA
SLILTVVCYLSVGPLFATPRTATVSYEIGFSSYFGTSSSSLLIYSAIYFAFVTVVSLYPNKLLDTVGHVLAPLKIIALAI
LGIAAVMIPAGYVPAPINHYVNSPVSEGFVNGYLTMDTLGALVFGIVIIQAIYSRGVTDNKLVTKYAIIASLISGVGLTL
VYLSLFKLGLGSHEVAPNAANGAVILHAYVQHAFGNMGSLFLTGLIFLACMVTAIGLTCACAEYFAQLTKLPYKLLVFIL
VGFSFIISNLGLTKLIAVSVPVLSAIYPPAIVVIMLSFFWKFWHKPSFIVGSVTSIALIFGIIDGLKVAGFGDYLPNFLQ
HLPLNEQNLAWLIPSLVVLVVTTLIDKVKHKSI
>Mature_433_residues
MQQLRTGDIIALGFMTFALFIGAGNIIFPPIVAQQAGDHVWLAAIGFLITAVGLPVITIMALSRMQGSIEIISSPLGRIA
SLILTVVCYLSVGPLFATPRTATVSYEIGFSSYFGTSSSSLLIYSAIYFAFVTVVSLYPNKLLDTVGHVLAPLKIIALAI
LGIAAVMIPAGYVPAPINHYVNSPVSEGFVNGYLTMDTLGALVFGIVIIQAIYSRGVTDNKLVTKYAIIASLISGVGLTL
VYLSLFKLGLGSHEVAPNAANGAVILHAYVQHAFGNMGSLFLTGLIFLACMVTAIGLTCACAEYFAQLTKLPYKLLVFIL
VGFSFIISNLGLTKLIAVSVPVLSAIYPPAIVVIMLSFFWKFWHKPSFIVGSVTSIALIFGIIDGLKVAGFGDYLPNFLQ
HLPLNEQNLAWLIPSLVVLVVTTLIDKVKHKSI

Specific function: Component of the LIV-III transport system for branched- chain amino acids. BraZ is specific for isoleucine and valine. The LIV-III transport system may be H(+)-coupled [H]

COG id: COG1114

COG function: function code E; Branched-chain amino acid permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the branched chain amino acid transporter family [H]

Homologues:

Organism=Escherichia coli, GI1786601, Length=432, Percent_Identity=59.0277777777778, Blast_Score=525, Evalue=1e-150,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004685 [H]

Pfam domain/function: PF05525 Branch_AA_trans [H]

EC number: NA

Molecular weight: Translated: 46237; Mature: 46237

Theoretical pI: Translated: 8.96; Mature: 8.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQLRTGDIIALGFMTFALFIGAGNIIFPPIVAQQAGDHVWLAAIGFLITAVGLPVITIM
CCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
ALSRMQGSIEIISSPLGRIASLILTVVCYLSVGPLFATPRTATVSYEIGFSSYFGTSSSS
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCHHHCCCCCCH
LLIYSAIYFAFVTVVSLYPNKLLDTVGHVLAPLKIIALAILGIAAVMIPAGYVPAPINHY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
VNSPVSEGFVNGYLTMDTLGALVFGIVIIQAIYSRGVTDNKLVTKYAIIASLISGVGLTL
HCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
VYLSLFKLGLGSHEVAPNAANGAVILHAYVQHAFGNMGSLFLTGLIFLACMVTAIGLTCA
HHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
CAEYFAQLTKLPYKLLVFILVGFSFIISNLGLTKLIAVSVPVLSAIYPPAIVVIMLSFFW
HHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KFWHKPSFIVGSVTSIALIFGIIDGLKVAGFGDYLPNFLQHLPLNEQNLAWLIPSLVVLV
HHHCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
VTTLIDKVKHKSI
HHHHHHHHHHCCC
>Mature Secondary Structure
MQQLRTGDIIALGFMTFALFIGAGNIIFPPIVAQQAGDHVWLAAIGFLITAVGLPVITIM
CCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
ALSRMQGSIEIISSPLGRIASLILTVVCYLSVGPLFATPRTATVSYEIGFSSYFGTSSSS
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCHHHCCCCCCH
LLIYSAIYFAFVTVVSLYPNKLLDTVGHVLAPLKIIALAILGIAAVMIPAGYVPAPINHY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
VNSPVSEGFVNGYLTMDTLGALVFGIVIIQAIYSRGVTDNKLVTKYAIIASLISGVGLTL
HCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
VYLSLFKLGLGSHEVAPNAANGAVILHAYVQHAFGNMGSLFLTGLIFLACMVTAIGLTCA
HHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
CAEYFAQLTKLPYKLLVFILVGFSFIISNLGLTKLIAVSVPVLSAIYPPAIVVIMLSFFW
HHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KFWHKPSFIVGSVTSIALIFGIIDGLKVAGFGDYLPNFLQHLPLNEQNLAWLIPSLVVLV
HHHCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
VTTLIDKVKHKSI
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-leucine [Periplasm]; L-valine [Periplasm]; Na (I) [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-leucine [Periplasm] = Na (I) [Cytoplasm] + L-leucine [Cytoplasm] Na (I) [Periplasm] + L-valine [Periplasm] = Na (I) [Cytoplasm] + L-valine [Cytoplasm] Na (I) [Periplasm] + L-iso-leucine [Periplasm] = Na (I) [Cytoplasm] + L-iso-leuci

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1900503; 10984043 [H]