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The map label for this gene is sdhA [C]

Identifier: 213157259

GI number: 213157259

Start: 2033804

End: 2035537

Strand: Direct

Name: sdhA [C]

Synonym: AB57_1946

Alternate gene names: 213157259

Gene position: 2033804-2035537 (Clockwise)

Preceding gene: 213157258

Following gene: 213157260

Centisome position: 50.21

GC content: 42.1

Gene sequence:

>1734_bases
ATGGAAAATAAATATCTTGAGTTCGATATTGTGGTGATTGGTGGTGGAACTGCAGGGCCGATGGCTGCGATTAAAGCCAA
ACAAGAAAACCCGAATTTAAAAGTCTTGCTCATTGAAAAAGCGAATGTAAAACGTAGTGGGGCGATTTCAATGGGAATGG
ATGGACTGAATAATGCTGTTATTCCGGGCTACGCAACACCTGAGCAATACACCAAAGAAATTACCATTGCTAATGATGGG
GTAGTTAACCAAACCACTGTATATGCATATGCGAAGCATAGTTTCACAACCATTCAACAGCTCGATCAATGGGGTATTAA
GTTTGAAAAAGATGAGACTGGCGAATTTGCAGTAAAAAAAGTACATCACATGGGGGCATATGTTTTACCAATGCCCGAAG
GCCATGACGTTAAAAAAGTTTTATATCGTCAGTTAAAGCGTGCTCAAGTCAAAATTAATAATCGGATTGTCACTACGAAG
CTTCTAAAAAATGAGCAAGGTGCAATCAATGGTGTACTTGGTTTTGACTGTCGTAGTGGTGACTTTTATGTCATTAAAAC
GAAAGCTGCCATTTTATGTTGTGGTGCCGCAGGGCGTTTAGGTTTGCCAGCTTCCGGCTATTTAATGGGCACTTATGAAA
ACCCAACCAATGCAGGTGACGGCTATGCAATGGCATACCATGCAGGTGCTGAGCTTGCTAACTTAGAATGCTTCCAGATT
AATCCATTGATTAAAGACTATAACGGCCCAGCTTGTGCCTATGTGACTGGTCCTTTAGGAGGTTATACCGCTAACAGTAA
AGGTGAACGTTTCATTGAATGTGACTACTGGAGTGGTCAAATGATGTGGGAGTTTTACCAAGAACTGGAAAGTGGTAATG
GTCCTGTTTATTTAAAAGTAGATCATCTGGCCGAAGAAACCATTCAAACCATCGAAGAAATCTTGCATACCAATGAGCGC
CCAAGCCGTGGACGTTTTCATGAGGGCCGAGGAACCAATTACCGTCAAGATATGGTGGAAATGCATATTTCAGAAATTGG
TTTCTGTAGCGGGCACAGCGCTTCTGGCGTATGGGTCAATGAAAAAGCTGAGACTTCAGTTAAAGGATTATACAGTGCTG
GCGATATGGCTGCTGTACCTCACAATTATATGCTTGGTGCATTTACCTACGGATGGTTTGCAGGTGTAAATGCCGCGCAA
TACGTGAATGCAGTTGAAGATAGTGAATTAAACCAAAGCGAAATAGAAGCAGAAAAAGCTCGAATATTTGCGCCACTGAA
TTGCTCAGAAGGTTTACCTCCAGAGCAAGTGGAATATAAGCTGCGCCGTTTTGTAAATGACTATTTGCAACCCCCTAAAA
CTCGCCAAAAAATGGAAATTGGGCTCAAGCGTTTTGAAGAAATCAAACAAGATATTAAGCGAATTTCTGCCAGAAACCCA
CATGAGCTGATGCGTGCCGCTGAAGTTTCTTTTATTCGAGATTGTGCGGAAATGGCAGCTAGAGCTTCACTGTTTAGAGA
GGAAAGCCGCTGGGGACTGTACCACTATAGAGCAGATTTTCCTCAAAAAAATAACTCGGATTGGTTCTGTCACGCGCATT
TGAAAAAAGATGAATACGGGAACATGGTCAGTTTCAAAAAGCCAATCGAGCCTTATGTGGTTCCGATTAATCAAGATGAA
GCTGTGTCGTATGACCGTTTACGCATTCAAAAAGATGTTGCTCTAGCAGACTAA

Upstream 100 bases:

>100_bases
CAACCGATTGACTATGAATATCTCTATTTCTCGGGCGATACCTTCCAGTACCGTTTACGTATTCATCGTTAATAACTCAA
ATAAAAATAAGGAATATTCA

Downstream 100 bases:

>100_bases
ATAAATCAGGAATTCAAATCATGGCTTTTATTTTTAAAGAAGTACAGCACCGTACTGTCGCACCCGTCATTATCGATGAA
GATAAATGTATTGCTGATAA

Product: putative oxidoreductase

Products: FADH2; Fumarate [C]

Alternate protein names: NA

Number of amino acids: Translated: 577; Mature: 577

Protein sequence:

>577_residues
MENKYLEFDIVVIGGGTAGPMAAIKAKQENPNLKVLLIEKANVKRSGAISMGMDGLNNAVIPGYATPEQYTKEITIANDG
VVNQTTVYAYAKHSFTTIQQLDQWGIKFEKDETGEFAVKKVHHMGAYVLPMPEGHDVKKVLYRQLKRAQVKINNRIVTTK
LLKNEQGAINGVLGFDCRSGDFYVIKTKAAILCCGAAGRLGLPASGYLMGTYENPTNAGDGYAMAYHAGAELANLECFQI
NPLIKDYNGPACAYVTGPLGGYTANSKGERFIECDYWSGQMMWEFYQELESGNGPVYLKVDHLAEETIQTIEEILHTNER
PSRGRFHEGRGTNYRQDMVEMHISEIGFCSGHSASGVWVNEKAETSVKGLYSAGDMAAVPHNYMLGAFTYGWFAGVNAAQ
YVNAVEDSELNQSEIEAEKARIFAPLNCSEGLPPEQVEYKLRRFVNDYLQPPKTRQKMEIGLKRFEEIKQDIKRISARNP
HELMRAAEVSFIRDCAEMAARASLFREESRWGLYHYRADFPQKNNSDWFCHAHLKKDEYGNMVSFKKPIEPYVVPINQDE
AVSYDRLRIQKDVALAD

Sequences:

>Translated_577_residues
MENKYLEFDIVVIGGGTAGPMAAIKAKQENPNLKVLLIEKANVKRSGAISMGMDGLNNAVIPGYATPEQYTKEITIANDG
VVNQTTVYAYAKHSFTTIQQLDQWGIKFEKDETGEFAVKKVHHMGAYVLPMPEGHDVKKVLYRQLKRAQVKINNRIVTTK
LLKNEQGAINGVLGFDCRSGDFYVIKTKAAILCCGAAGRLGLPASGYLMGTYENPTNAGDGYAMAYHAGAELANLECFQI
NPLIKDYNGPACAYVTGPLGGYTANSKGERFIECDYWSGQMMWEFYQELESGNGPVYLKVDHLAEETIQTIEEILHTNER
PSRGRFHEGRGTNYRQDMVEMHISEIGFCSGHSASGVWVNEKAETSVKGLYSAGDMAAVPHNYMLGAFTYGWFAGVNAAQ
YVNAVEDSELNQSEIEAEKARIFAPLNCSEGLPPEQVEYKLRRFVNDYLQPPKTRQKMEIGLKRFEEIKQDIKRISARNP
HELMRAAEVSFIRDCAEMAARASLFREESRWGLYHYRADFPQKNNSDWFCHAHLKKDEYGNMVSFKKPIEPYVVPINQDE
AVSYDRLRIQKDVALAD
>Mature_577_residues
MENKYLEFDIVVIGGGTAGPMAAIKAKQENPNLKVLLIEKANVKRSGAISMGMDGLNNAVIPGYATPEQYTKEITIANDG
VVNQTTVYAYAKHSFTTIQQLDQWGIKFEKDETGEFAVKKVHHMGAYVLPMPEGHDVKKVLYRQLKRAQVKINNRIVTTK
LLKNEQGAINGVLGFDCRSGDFYVIKTKAAILCCGAAGRLGLPASGYLMGTYENPTNAGDGYAMAYHAGAELANLECFQI
NPLIKDYNGPACAYVTGPLGGYTANSKGERFIECDYWSGQMMWEFYQELESGNGPVYLKVDHLAEETIQTIEEILHTNER
PSRGRFHEGRGTNYRQDMVEMHISEIGFCSGHSASGVWVNEKAETSVKGLYSAGDMAAVPHNYMLGAFTYGWFAGVNAAQ
YVNAVEDSELNQSEIEAEKARIFAPLNCSEGLPPEQVEYKLRRFVNDYLQPPKTRQKMEIGLKRFEEIKQDIKRISARNP
HELMRAAEVSFIRDCAEMAARASLFREESRWGLYHYRADFPQKNNSDWFCHAHLKKDEYGNMVSFKKPIEPYVVPINQDE
AVSYDRLRIQKDVALAD

Specific function: Two Distinct, Membrane-Bound, FAD-Containing Enzymes Are Responsible For The Catalysis Of Fumarate And Succinate Interconversion; The Fumarate Reductase Is Used In Anaerobic Growth, And The Succinate Dehydrogenase Is Used In Aerobic Growth. [C]

COG id: COG1053

COG function: function code C; Succinate dehydrogenase/fumarate reductase, flavoprotein subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786942, Length=459, Percent_Identity=25.4901960784314, Blast_Score=105, Evalue=1e-23,
Organism=Escherichia coli, GI1790597, Length=561, Percent_Identity=20.1426024955437, Blast_Score=70, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6322701, Length=500, Percent_Identity=20.2, Blast_Score=64, Evalue=5e-11,

Paralogues:

None

Copy number: 1900 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1360 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 1100 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: 1.3.99.1 [C]

Molecular weight: Translated: 64717; Mature: 64717

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENKYLEFDIVVIGGGTAGPMAAIKAKQENPNLKVLLIEKANVKRSGAISMGMDGLNNAV
CCCCEEEEEEEEECCCCCCCHHEEEECCCCCCEEEEEEECCCCCCCCCEEECCCCCCCCC
IPGYATPEQYTKEITIANDGVVNQTTVYAYAKHSFTTIQQLDQWGIKFEKDETGEFAVKK
CCCCCCHHHHCCEEEEECCCCCCCEEEEEEECCCHHHHHHHHHHCCEEECCCCCHHHHHH
VHHMGAYVLPMPEGHDVKKVLYRQLKRAQVKINNRIVTTKLLKNEQGAINGVLGFDCRSG
HHHCCEEEEECCCCCCHHHHHHHHHHHHHEEECCEEEEEHHHCCCCCCCCCEEEEEECCC
DFYVIKTKAAILCCGAAGRLGLPASGYLMGTYENPTNAGDGYAMAYHAGAELANLECFQI
CEEEEEECEEEEEECCCCCCCCCCCCEEEEEECCCCCCCCCEEEEEECCCCCCCEEEEEE
NPLIKDYNGPACAYVTGPLGGYTANSKGERFIECDYWSGQMMWEFYQELESGNGPVYLKV
CCHHHCCCCCEEEEEECCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCEEEEE
DHLAEETIQTIEEILHTNERPSRGRFHEGRGTNYRQDMVEMHISEIGFCSGHSASGVWVN
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEC
EKAETSVKGLYSAGDMAAVPHNYMLGAFTYGWFAGVNAAQYVNAVEDSELNQSEIEAEKA
CCCCHHHHHHHCCCCCCCCCCCCEEHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHH
RIFAPLNCSEGLPPEQVEYKLRRFVNDYLQPPKTRQKMEIGLKRFEEIKQDIKRISARNP
EEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCH
HELMRAAEVSFIRDCAEMAARASLFREESRWGLYHYRADFPQKNNSDWFCHAHLKKDEYG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEEEEECCCCCCC
NMVSFKKPIEPYVVPINQDEAVSYDRLRIQKDVALAD
CEEEECCCCCCEEEECCCCCCCCHHHHEEECCCCCCC
>Mature Secondary Structure
MENKYLEFDIVVIGGGTAGPMAAIKAKQENPNLKVLLIEKANVKRSGAISMGMDGLNNAV
CCCCEEEEEEEEECCCCCCCHHEEEECCCCCCEEEEEEECCCCCCCCCEEECCCCCCCCC
IPGYATPEQYTKEITIANDGVVNQTTVYAYAKHSFTTIQQLDQWGIKFEKDETGEFAVKK
CCCCCCHHHHCCEEEEECCCCCCCEEEEEEECCCHHHHHHHHHHCCEEECCCCCHHHHHH
VHHMGAYVLPMPEGHDVKKVLYRQLKRAQVKINNRIVTTKLLKNEQGAINGVLGFDCRSG
HHHCCEEEEECCCCCCHHHHHHHHHHHHHEEECCEEEEEHHHCCCCCCCCCEEEEEECCC
DFYVIKTKAAILCCGAAGRLGLPASGYLMGTYENPTNAGDGYAMAYHAGAELANLECFQI
CEEEEEECEEEEEECCCCCCCCCCCCEEEEEECCCCCCCCCEEEEEECCCCCCCEEEEEE
NPLIKDYNGPACAYVTGPLGGYTANSKGERFIECDYWSGQMMWEFYQELESGNGPVYLKV
CCHHHCCCCCEEEEEECCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCEEEEE
DHLAEETIQTIEEILHTNERPSRGRFHEGRGTNYRQDMVEMHISEIGFCSGHSASGVWVN
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEC
EKAETSVKGLYSAGDMAAVPHNYMLGAFTYGWFAGVNAAQYVNAVEDSELNQSEIEAEKA
CCCCHHHHHHHCCCCCCCCCCCCEEHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHH
RIFAPLNCSEGLPPEQVEYKLRRFVNDYLQPPKTRQKMEIGLKRFEEIKQDIKRISARNP
EEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCH
HELMRAAEVSFIRDCAEMAARASLFREESRWGLYHYRADFPQKNNSDWFCHAHLKKDEYG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEEEEECCCCCCC
NMVSFKKPIEPYVVPINQDEAVSYDRLRIQKDVALAD
CEEEECCCCCCEEEECCCCCCCCHHHHEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: ATP; Dithiothreitol; FAD; Fe-S-clusters [C]

Metal ions: Anions; Diphosphate; Fe [C]

Kcat value (1/min): 10000-13000 [C]

Specific activity: 509

Km value (mM): 0.02 {succinate}} 0.4 {fumarate}} 0.3 {ferricyanide}} [C]

Substrates: FAD; Succinate [C]

Specific reaction: FAD + Succinate --> FADH2 + Fumarate [C]

General reaction: Redox reaction [C]

Inhibitor: 5, 5'-Dithiobis (2-nitrobenzoate); Chaotropic reagents [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]