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The map label for this gene is acoA [H]

Identifier: 213157241

GI number: 213157241

Start: 2014490

End: 2015452

Strand: Direct

Name: acoA [H]

Synonym: AB57_1928

Alternate gene names: 213157241

Gene position: 2014490-2015452 (Clockwise)

Preceding gene: 213157238

Following gene: 213157242

Centisome position: 49.73

GC content: 44.13

Gene sequence:

>963_bases
ATGCAATTAACGGAAGAGCAATTACTCGCAGCATATAAACGCATGCGTGATATTCGTGAATTTGAAGATCGACTTCATGA
AGAAAATACCAATGGTGATATTCCAGGCTTTATTCACTTATATAGCGGAGAAGAAGCTGTAGCAGTCGGGATTTGCGAAA
ACTTAACCGACAAAGACTATATCACTTCAACACACCGTGGACATGGACACTGTATTGCAAAAGGCTGTGATATTCACGCC
ATGATGCTGGAAATTTTCGGTAAAGATGACGGACTATGCCGTGGAAAAGGCGGTTCTATGCATATTGCCGATTTAGATAA
AGGTATGCTCGGTGCCAACGGGATTGTGGGTGGTGGGCCTCCTTTAGCAATTGGTGCAGCGTTGACAGCGAAAACCTTAA
AGACTGGCGGCGTCGGACTGTCTTTTACAGGTGATGGTGGTTCAAACCAAGGCCTTACTTTTGAAGCGATGAACATGGCT
GTTGTTCTTAAACTTCCCGTGATTTTCGTTTTTGAAAATAACGGTTTTGGTGAGGGGACTGGTCATGATTACGCTGTAGG
AAGCAAAGACATTGCTGGTCGTGCAGCAGGCTTTGGTTTACCGGCAGTTAAAGTTGATGGTACAGACTTCTTTGCTGTCT
ATGAAGCCGCTCAAACTGCCATTGAACGCGCGCGCCGTGGTGAAGGTCCAAGCGTGATTGAAACCATTACAAACCGTTTT
TATGGCCACTTCGAAGGTGACCCTGGCTTGATTCGTTCTAAAGAAGAAGTCGAGTTCATCAAAGAAAATAAAGATCCTTT
GAAAATTTTCCGCGAAAAAGTTAAAGGCAAAATTGATGAAGCCAAACTAGACGAGATTGATGCAGCTTCTAAGGCAAATG
TTGATGATGCCGTTGCCAAAGCTCGTGCTGCGGCTTATCCAAAACCAGAACAACTCCTTACTGACGTCTATGTCTCTTAC
TAA

Upstream 100 bases:

>100_bases
CATATTAAATTAAATTGAGCTTTATTATTTATAGCGAAATATATAACACTCGCTGAAACTTGGTTGATTTTTAATAATCA
AGATAATAAAGGAAAAGGAT

Downstream 100 bases:

>100_bases
AGGAATAGTAAAGATGCCAAATAAAAGTTTTCGTAATGCAATTAAAGAAGCCATTGAATCAGAAATGCGCCGTGATCCAA
CCGTATTTGTTGTTGGTGAA

Product: acetoin:2,6-dichlorophenolindophenol oxidoreductase alpha subunit

Products: [dihydrolipoyllysine-residue acetyltransferase] ; $S-acetyldihydrolipoyllysine; CO2

Alternate protein names: Acetoin:DCPIP oxidoreductase-alpha; Ao:DCPIP OR; TPP-dependent acetoin dehydrogenase E1 subunit alpha [H]

Number of amino acids: Translated: 320; Mature: 320

Protein sequence:

>320_residues
MQLTEEQLLAAYKRMRDIREFEDRLHEENTNGDIPGFIHLYSGEEAVAVGICENLTDKDYITSTHRGHGHCIAKGCDIHA
MMLEIFGKDDGLCRGKGGSMHIADLDKGMLGANGIVGGGPPLAIGAALTAKTLKTGGVGLSFTGDGGSNQGLTFEAMNMA
VVLKLPVIFVFENNGFGEGTGHDYAVGSKDIAGRAAGFGLPAVKVDGTDFFAVYEAAQTAIERARRGEGPSVIETITNRF
YGHFEGDPGLIRSKEEVEFIKENKDPLKIFREKVKGKIDEAKLDEIDAASKANVDDAVAKARAAAYPKPEQLLTDVYVSY

Sequences:

>Translated_320_residues
MQLTEEQLLAAYKRMRDIREFEDRLHEENTNGDIPGFIHLYSGEEAVAVGICENLTDKDYITSTHRGHGHCIAKGCDIHA
MMLEIFGKDDGLCRGKGGSMHIADLDKGMLGANGIVGGGPPLAIGAALTAKTLKTGGVGLSFTGDGGSNQGLTFEAMNMA
VVLKLPVIFVFENNGFGEGTGHDYAVGSKDIAGRAAGFGLPAVKVDGTDFFAVYEAAQTAIERARRGEGPSVIETITNRF
YGHFEGDPGLIRSKEEVEFIKENKDPLKIFREKVKGKIDEAKLDEIDAASKANVDDAVAKARAAAYPKPEQLLTDVYVSY
>Mature_320_residues
MQLTEEQLLAAYKRMRDIREFEDRLHEENTNGDIPGFIHLYSGEEAVAVGICENLTDKDYITSTHRGHGHCIAKGCDIHA
MMLEIFGKDDGLCRGKGGSMHIADLDKGMLGANGIVGGGPPLAIGAALTAKTLKTGGVGLSFTGDGGSNQGLTFEAMNMA
VVLKLPVIFVFENNGFGEGTGHDYAVGSKDIAGRAAGFGLPAVKVDGTDFFAVYEAAQTAIERARRGEGPSVIETITNRF
YGHFEGDPGLIRSKEEVEFIKENKDPLKIFREKVKGKIDEAKLDEIDAASKANVDDAVAKARAAAYPKPEQLLTDVYVSY

Specific function: Catalyzes the 2,6-dichlorophenolindophenol-dependent cleavage of acetoin into acetate and acetaldehyde. The alpha subunit is probably the catalytic subunit of the enzyme [H]

COG id: COG1071

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, alpha subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI4885543, Length=320, Percent_Identity=36.25, Blast_Score=201, Evalue=1e-51,
Organism=Homo sapiens, GI291084742, Length=320, Percent_Identity=35.9375, Blast_Score=182, Evalue=4e-46,
Organism=Homo sapiens, GI4505685, Length=320, Percent_Identity=35.9375, Blast_Score=182, Evalue=5e-46,
Organism=Homo sapiens, GI291084744, Length=327, Percent_Identity=35.1681957186544, Blast_Score=175, Evalue=5e-44,
Organism=Homo sapiens, GI291084757, Length=320, Percent_Identity=32.1875, Blast_Score=148, Evalue=5e-36,
Organism=Homo sapiens, GI11386135, Length=318, Percent_Identity=27.9874213836478, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI258645172, Length=318, Percent_Identity=27.9874213836478, Blast_Score=119, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI32564172, Length=318, Percent_Identity=37.7358490566038, Blast_Score=204, Evalue=4e-53,
Organism=Caenorhabditis elegans, GI17536047, Length=318, Percent_Identity=37.7358490566038, Blast_Score=204, Evalue=6e-53,
Organism=Caenorhabditis elegans, GI86563357, Length=317, Percent_Identity=28.0757097791798, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI86563355, Length=317, Percent_Identity=28.0757097791798, Blast_Score=119, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6321026, Length=324, Percent_Identity=36.1111111111111, Blast_Score=186, Evalue=4e-48,
Organism=Drosophila melanogaster, GI24639740, Length=323, Percent_Identity=40.5572755417957, Blast_Score=208, Evalue=4e-54,
Organism=Drosophila melanogaster, GI24639744, Length=323, Percent_Identity=40.5572755417957, Blast_Score=208, Evalue=4e-54,
Organism=Drosophila melanogaster, GI28571106, Length=323, Percent_Identity=40.5572755417957, Blast_Score=208, Evalue=4e-54,
Organism=Drosophila melanogaster, GI24639748, Length=320, Percent_Identity=38.125, Blast_Score=199, Evalue=3e-51,
Organism=Drosophila melanogaster, GI24639746, Length=309, Percent_Identity=40.7766990291262, Blast_Score=197, Evalue=7e-51,
Organism=Drosophila melanogaster, GI21355903, Length=321, Percent_Identity=27.7258566978193, Blast_Score=106, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001017 [H]

Pfam domain/function: PF00676 E1_dh [H]

EC number: 1.2.4.1

Molecular weight: Translated: 34375; Mature: 34375

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLTEEQLLAAYKRMRDIREFEDRLHEENTNGDIPGFIHLYSGEEAVAVGICENLTDKDY
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCEEEEEEHHCCCCCCC
ITSTHRGHGHCIAKGCDIHAMMLEIFGKDDGLCRGKGGSMHIADLDKGMLGANGIVGGGP
CCCCCCCCCEEEECCCCHHHHHHHHHCCCCCEEECCCCCEEEEECCCCCCCCCCCCCCCC
PLAIGAALTAKTLKTGGVGLSFTGDGGSNQGLTFEAMNMAVVLKLPVIFVFENNGFGEGT
CHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEHHCEEEEEEEEEEEEECCCCCCCCC
GHDYAVGSKDIAGRAAGFGLPAVKVDGTDFFAVYEAAQTAIERARRGEGPSVIETITNRF
CCCCCCCCCHHCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
YGHFEGDPGLIRSKEEVEFIKENKDPLKIFREKVKGKIDEAKLDEIDAASKANVDDAVAK
CEEECCCCCCCCCHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHCCCCCCCHHHHHHH
ARAAAYPKPEQLLTDVYVSY
HHHHCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MQLTEEQLLAAYKRMRDIREFEDRLHEENTNGDIPGFIHLYSGEEAVAVGICENLTDKDY
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCEEEEEEHHCCCCCCC
ITSTHRGHGHCIAKGCDIHAMMLEIFGKDDGLCRGKGGSMHIADLDKGMLGANGIVGGGP
CCCCCCCCCEEEECCCCHHHHHHHHHCCCCCEEECCCCCEEEEECCCCCCCCCCCCCCCC
PLAIGAALTAKTLKTGGVGLSFTGDGGSNQGLTFEAMNMAVVLKLPVIFVFENNGFGEGT
CHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEHHCEEEEEEEEEEEEECCCCCCCCC
GHDYAVGSKDIAGRAAGFGLPAVKVDGTDFFAVYEAAQTAIERARRGEGPSVIETITNRF
CCCCCCCCCHHCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
YGHFEGDPGLIRSKEEVEFIKENKDPLKIFREKVKGKIDEAKLDEIDAASKANVDDAVAK
CEEECCCCCCCCCHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHCCCCCCCHHHHHHH
ARAAAYPKPEQLLTDVYVSY
HHHHCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: pyruvate; [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine

Specific reaction: pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S- $acetyldihydrolipoyllysine + CO2

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969503; 10368162; 9384377 [H]