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The map label for this gene is metZ [H]

Identifier: 213157223

GI number: 213157223

Start: 1996868

End: 1998079

Strand: Direct

Name: metZ [H]

Synonym: AB57_1907

Alternate gene names: 213157223

Gene position: 1996868-1998079 (Clockwise)

Preceding gene: 213157221

Following gene: 213157224

Centisome position: 49.3

GC content: 40.35

Gene sequence:

>1212_bases
ATGACCAGTAAAGGCGAACATTTAATGAACCAGTCTGATGATTTAGAATACCAACTTGATACTTTAGCCATTCGTACCGG
ACACACTCGTAGTTTTGAAGGTGAACATGGCGAACCAATTTTTTTGACTTCATCTTTTGTATATGAAAATGCTGCTGAAG
CAGCTGCAAAATTTTCTGGTCAGGTTCAAGGAAATATCTATTCTCGTTTTACTAATCCGACTGTATCGATGTTCGAAAAA
CGTTTAGCGGCTTTAGATGGGGCTGAACGTGCCGTGGCTACAAGCTCAGGTATGGGAGCAATTCTATCGATTGCGATGGC
GTATCTAAAAGCAGGTGATCATGTTATTTGCTCAAGAGCAGTTTTCGGTTCTATTGTCGCTTTATTTGAAAAATATATTG
CCAAATTTGGTGTAGATATCACATTTGTGGATCTACGCGATTTAGATGCATGGAAAAATGCAGTACGTCCAGAAACTAAA
CTATTATTTGTTGAGTCACCGTCTAATCCATTAGCTGAAATCGCAGATATCCAAGCGCTTGCAGATATTGCGCATGCAAA
TGGCGCATTACTTGCTGTTGACAATAGTTTCTGTACACCTGTTTTACAGCAACCGTTAAAGTTGGGTGCAGATCTCGTGA
TTTACTCTGCGACTAAATATTTAGATGGCCAAGGCCGTGCGTTAGGTGGTGCAGTGGTAGGTAACCATCAACTCCTAGAA
GAAGTTTTTAGCGTAGTGCGTACTTTAGGGCCATCTATGAGTCCGTTTAATGCATGGATTTTCTTAAAAGGATTAGAAAC
TTTACGTTTACGTATGAAAGCGCACTGTGAAAATGCTCAAAAACTTGCCGAGTGGTTGTCTACCCATGAAAAAGTAGAAA
AGGTCTATTATGGCGGGCTTCCAACTCATGAAGGTCATGAACTTGCAGCAAAACAGCAAAATGGCTTTGGCGGGATTGTT
TCTTTTGTAGTTAAAGGTGAGCGTGAAGGCGCATGGAAGGTTATTGATAACACCAAATTTATTTCTATCACGGGTAATTT
AGGTGATGTGAAATCAACAATTACGCATCCTGCTACAACCACACACGGCAAACTTTCTGCTGAAGCAAAACAAGCTGCTG
GTATTGAAGAAGGCTTAATCCGCGTTTCGGTTGGCTTAGAAGATATTAACGATATTATTGGTGATATCTCACGTGGATTA
GATTTGATCTAA

Upstream 100 bases:

>100_bases
CCAACTATACTAAAGATTGATTGAAGTCTAAGCGATAATTTTCAGGTTTACCTTGACCTTAAATACCTTAGGCACTCAAA
ATGATGATAGTTTTTAGTAC

Downstream 100 bases:

>100_bases
TAAAATTTATATATTTAAGAATTAAGCCACACTCATTTTAGTGTGGCTTTACTTTAAGGTAAGTTACTCACAAACAACGA
TTGAAATAAAAAAGCTACAT

Product: O-succinylhomoserine sulfhydrylase

Products: Cystathionine; succinate; o-succinyl-L-homoserine; H2O [C]

Alternate protein names: OSH sulfhydrylase [H]

Number of amino acids: Translated: 403; Mature: 402

Protein sequence:

>403_residues
MTSKGEHLMNQSDDLEYQLDTLAIRTGHTRSFEGEHGEPIFLTSSFVYENAAEAAAKFSGQVQGNIYSRFTNPTVSMFEK
RLAALDGAERAVATSSGMGAILSIAMAYLKAGDHVICSRAVFGSIVALFEKYIAKFGVDITFVDLRDLDAWKNAVRPETK
LLFVESPSNPLAEIADIQALADIAHANGALLAVDNSFCTPVLQQPLKLGADLVIYSATKYLDGQGRALGGAVVGNHQLLE
EVFSVVRTLGPSMSPFNAWIFLKGLETLRLRMKAHCENAQKLAEWLSTHEKVEKVYYGGLPTHEGHELAAKQQNGFGGIV
SFVVKGEREGAWKVIDNTKFISITGNLGDVKSTITHPATTTHGKLSAEAKQAAGIEEGLIRVSVGLEDINDIIGDISRGL
DLI

Sequences:

>Translated_403_residues
MTSKGEHLMNQSDDLEYQLDTLAIRTGHTRSFEGEHGEPIFLTSSFVYENAAEAAAKFSGQVQGNIYSRFTNPTVSMFEK
RLAALDGAERAVATSSGMGAILSIAMAYLKAGDHVICSRAVFGSIVALFEKYIAKFGVDITFVDLRDLDAWKNAVRPETK
LLFVESPSNPLAEIADIQALADIAHANGALLAVDNSFCTPVLQQPLKLGADLVIYSATKYLDGQGRALGGAVVGNHQLLE
EVFSVVRTLGPSMSPFNAWIFLKGLETLRLRMKAHCENAQKLAEWLSTHEKVEKVYYGGLPTHEGHELAAKQQNGFGGIV
SFVVKGEREGAWKVIDNTKFISITGNLGDVKSTITHPATTTHGKLSAEAKQAAGIEEGLIRVSVGLEDINDIIGDISRGL
DLI
>Mature_402_residues
TSKGEHLMNQSDDLEYQLDTLAIRTGHTRSFEGEHGEPIFLTSSFVYENAAEAAAKFSGQVQGNIYSRFTNPTVSMFEKR
LAALDGAERAVATSSGMGAILSIAMAYLKAGDHVICSRAVFGSIVALFEKYIAKFGVDITFVDLRDLDAWKNAVRPETKL
LFVESPSNPLAEIADIQALADIAHANGALLAVDNSFCTPVLQQPLKLGADLVIYSATKYLDGQGRALGGAVVGNHQLLEE
VFSVVRTLGPSMSPFNAWIFLKGLETLRLRMKAHCENAQKLAEWLSTHEKVEKVYYGGLPTHEGHELAAKQQNGFGGIVS
FVVKGEREGAWKVIDNTKFISITGNLGDVKSTITHPATTTHGKLSAEAKQAAGIEEGLIRVSVGLEDINDIIGDISRGLD
LI

Specific function: Converts O-succinylhomoserine into homocysteine [H]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=328, Percent_Identity=39.3292682926829, Blast_Score=241, Evalue=7e-64,
Organism=Homo sapiens, GI299473759, Length=328, Percent_Identity=35.3658536585366, Blast_Score=203, Evalue=3e-52,
Organism=Homo sapiens, GI25453487, Length=327, Percent_Identity=33.9449541284404, Blast_Score=181, Evalue=8e-46,
Organism=Escherichia coli, GI1790375, Length=382, Percent_Identity=36.3874345549738, Blast_Score=249, Evalue=2e-67,
Organism=Escherichia coli, GI1789383, Length=401, Percent_Identity=27.930174563591, Blast_Score=153, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI17538055, Length=353, Percent_Identity=36.5439093484419, Blast_Score=241, Evalue=6e-64,
Organism=Caenorhabditis elegans, GI17560056, Length=353, Percent_Identity=35.6940509915014, Blast_Score=235, Evalue=3e-62,
Organism=Caenorhabditis elegans, GI71980708, Length=368, Percent_Identity=33.695652173913, Blast_Score=219, Evalue=2e-57,
Organism=Saccharomyces cerevisiae, GI6319307, Length=390, Percent_Identity=34.8717948717949, Blast_Score=224, Evalue=2e-59,
Organism=Saccharomyces cerevisiae, GI6323333, Length=436, Percent_Identity=33.256880733945, Blast_Score=217, Evalue=2e-57,
Organism=Saccharomyces cerevisiae, GI6321254, Length=404, Percent_Identity=26.2376237623762, Blast_Score=145, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6321170, Length=311, Percent_Identity=28.6173633440514, Blast_Score=135, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6321904, Length=342, Percent_Identity=29.8245614035088, Blast_Score=126, Evalue=7e-30,
Organism=Drosophila melanogaster, GI19922554, Length=367, Percent_Identity=38.1471389645777, Blast_Score=261, Evalue=5e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006234
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: 2.5.1.48 [C]

Molecular weight: Translated: 43552; Mature: 43421

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSKGEHLMNQSDDLEYQLDTLAIRTGHTRSFEGEHGEPIFLTSSFVYENAAEAAAKFSG
CCCCCCHHCCCCCCCEEEEEEEEEECCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHCC
QVQGNIYSRFTNPTVSMFEKRLAALDGAERAVATSSGMGAILSIAMAYLKAGDHVICSRA
CCCCCHHHHCCCCHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHCCCCEEEHHH
VFGSIVALFEKYIAKFGVDITFVDLRDLDAWKNAVRPETKLLFVESPSNPLAEIADIQAL
HHHHHHHHHHHHHHHHCCCEEEEECHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHH
ADIAHANGALLAVDNSFCTPVLQQPLKLGADLVIYSATKYLDGQGRALGGAVVGNHQLLE
HHHHHCCCCEEEECCCCCCHHHHHHHHCCCCEEEEEHHHHCCCCCCEECCEEECCHHHHH
EVFSVVRTLGPSMSPFNAWIFLKGLETLRLRMKAHCENAQKLAEWLSTHEKVEKVYYGGL
HHHHHHHHHCCCCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PTHEGHELAAKQQNGFGGIVSFVVKGEREGAWKVIDNTKFISITGNLGDVKSTITHPATT
CCCCCCHHHHHCCCCCCHHHHHEEECCCCCCEEEECCCEEEEEECCCCHHHHHHCCCCCC
THGKLSAEAKQAAGIEEGLIRVSVGLEDINDIIGDISRGLDLI
CCCCCCHHHHHHCCHHHCEEEEEECHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TSKGEHLMNQSDDLEYQLDTLAIRTGHTRSFEGEHGEPIFLTSSFVYENAAEAAAKFSG
CCCCCHHCCCCCCCEEEEEEEEEECCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHCC
QVQGNIYSRFTNPTVSMFEKRLAALDGAERAVATSSGMGAILSIAMAYLKAGDHVICSRA
CCCCCHHHHCCCCHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHCCCCEEEHHH
VFGSIVALFEKYIAKFGVDITFVDLRDLDAWKNAVRPETKLLFVESPSNPLAEIADIQAL
HHHHHHHHHHHHHHHHCCCEEEEECHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHH
ADIAHANGALLAVDNSFCTPVLQQPLKLGADLVIYSATKYLDGQGRALGGAVVGNHQLLE
HHHHHCCCCEEEECCCCCCHHHHHHHHCCCCEEEEEHHHHCCCCCCEECCEEECCHHHHH
EVFSVVRTLGPSMSPFNAWIFLKGLETLRLRMKAHCENAQKLAEWLSTHEKVEKVYYGGL
HHHHHHHHHCCCCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PTHEGHELAAKQQNGFGGIVSFVVKGEREGAWKVIDNTKFISITGNLGDVKSTITHPATT
CCCCCCHHHHHCCCCCCHHHHHEEECCCCCCEEEECCCEEEEEECCCCHHHHHHCCCCCC
THGKLSAEAKQAAGIEEGLIRVSVGLEDINDIIGDISRGLDLI
CCCCCCHHHHHHCCHHHCEEEEEECHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: pyridoxal 5'-phosphate [C]

Metal ions: NA

Kcat value (1/min): 460 [C]

Specific activity: 10

Km value (mM): 0.33 {O-succinyl-L-homoserine}} [C]

Substrates: O-succinyl-L-homoserine; @CYST01.txt*L-cysteine!; 2-oxobutanoate; succinate; NH3 [C]

Specific reaction: O-succinyl-L-homoserine + @CYST01.txt*L-cysteine! = cystathionine + succinate 2-oxobutanoate + succinate + NH3 = o-succinyl-L-homoserine + H2O [C]

General reaction: Elimination; Gamma-replacement [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7704274; 10984043 [H]