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The map label for this gene is oppA [H]

Identifier: 213157150

GI number: 213157150

Start: 1918812

End: 1920632

Strand: Direct

Name: oppA [H]

Synonym: AB57_1833

Alternate gene names: 213157150

Gene position: 1918812-1920632 (Clockwise)

Preceding gene: 213157149

Following gene: 213157151

Centisome position: 47.37

GC content: 40.2

Gene sequence:

>1821_bases
ATGACCGAAAATTTAAAACAAAAGCTTAAGCACTGTAGTGTCGGCCTATTCTTTGCCGGAATGGCTTTAACGGGTTCTAT
ATCTATTTTTGCGAAAAGCCCAGCCGATCCAAATAAAGTTTTGCGTTATGTATTCCCAACAGCTGAAACAGGCTTCGACC
CAGCTTATGTACATGACTTATATTCAGCGCATGTACTCACATCTATATTTGAAACTTTATATACCTATGACTATTTGGCA
AGACCAGCAAAATTAATCCCACACGTGGCTACGGCTATGCCAGAAGTGAGTGCAGATGGTCTGACTTATACCATTCATAT
TAAGAAGGGTATTTACTTTACAGCCGACCCAGCGTTTAAAGGTAAACCTAGAGAGTTAACAGCTTACGATTATGCATATT
CTTTTAAGCGGTTATTAGACCCGAATTTACGCTCACCCAATAGTTGGTTATTGGAAGATAAAATTGAAGGTATGAATGCT
TTAGTCAAAGCAGCCAATAAATCCGGAAAATTTAATTACGATCAAAATGTAAGTGGTTTACAAACACCTGATAAGTACAC
TTTGGTGATTCGGTTAGTAAAACCAGACTATAACTTTCCTTTATTACTGGCCCATGATCCGACAGGTGCGGTGGCACGCG
AAGTCATCGAGAAATATAAAGATAAAGCTGGTTTTGTTATGGGGCATCCAGTAGGAACTGGACCTTATATGCTCAGTAAG
TGGATACCTGCCTCACGTATTGTTTTAAAAGCAAATCCTGAATATCGAGGTTTTATCTGGAACTTTAATGCCAGTAGCCC
CGGGGATGAAGCAATTGTAAAGCGCCTTAAAGGTAAACAAATGCCTCAAATCGGCACAATTGATATTCAGGTCATGGAAG
AAAACCAGTCACGATGGTTAGCATTCCAGCGTGGTGAGGTAGATATCATTCAGCTTGAAGGGCAATTAGTTTCAAAAGCG
ATTAAAGACGGTAAATTAAGACCCGAGCTTGCAAAAGAAGGCGTGCAATTATCTCGTATTGTTGATCCAGAAATTAGTTA
TATCTATTGGAATCTCAAAGATCCCGTTGTTGGAGGAATGAGTAAAGAAAAAATTGCCTTACGCCGTGCTATTGCGATGT
CCCGTTCAATAGATCAAGAGATTAAATTGGTAAGGAATAGTGATGCAGAACGTCTTCATTTCCCAGTTCCACCTGGTGTA
GTGGGATATGACCCACAGTATAGAAGCAGTACACCATATTCAGTAAAAGCAGCCAATCTGCTGTTGGATCGATATCACTA
CAAAAAAGATGCTTCTGGTTGGCGTACACAACCAAACGGCAAGCCGCTCGTGGTCGAATATAAAGCGCGCAATGACAGTA
TTGGCCAACAAAGTGCTGAACTTTGGAAGAAAAACTTCGATAGCCTACATATTCGCATGGTGTATAAGCCAATGCTTTTT
TCAGACTTACTTCGATCACAGAAACAATGTGAGGGAATGTTTGGTTCTTCTGCATGGATAGCTGATTATCCGGATGGGGA
TAATTTTATGCAGAATTTTTATGGTCCAAATACCCATATGACTAACTGGTCTTGTGGTTCGATACCAGAGTTTGATGAGC
TGTACCGCCAGTCTCAACAAGTGAAGCCAGGCCCAGAAAGAGATGCGTTATATCGAAAAATGACGCGCTTGTTAGAGGTT
TATATGCCTGTGCAAATGTCTTATGCACGCTATCGCAATATGTTGGCCCAACCACGAATTATTGGATATAAGAAGCATCC
GATTTTACATGCTGAATGGATGTACTTCGATATTGATACAAATATGAAATCTAATCATTAA

Upstream 100 bases:

>100_bases
GCCTCAAGTTCAGGGGTTTAAAGCTCACCCAATGATGAATACAAACTGGCAATATCTTGATATTACCCCTATTAAAAAAT
AATTTTAGATAAAGATCATC

Downstream 100 bases:

>100_bases
ATGAAAAAAATTGGAGATACGATGAAAAATAATACACTGAAAATGACTACATTATGTATGTTGACCATAGGTATAAGTCA
ATTTGCATCGGCTGAAGCGA

Product: heme-binding protein A

Products: ADP; phosphate; oligopeptides [Cytoplasm] [C]

Alternate protein names: Stage 0 sporulation protein KA [H]

Number of amino acids: Translated: 606; Mature: 605

Protein sequence:

>606_residues
MTENLKQKLKHCSVGLFFAGMALTGSISIFAKSPADPNKVLRYVFPTAETGFDPAYVHDLYSAHVLTSIFETLYTYDYLA
RPAKLIPHVATAMPEVSADGLTYTIHIKKGIYFTADPAFKGKPRELTAYDYAYSFKRLLDPNLRSPNSWLLEDKIEGMNA
LVKAANKSGKFNYDQNVSGLQTPDKYTLVIRLVKPDYNFPLLLAHDPTGAVAREVIEKYKDKAGFVMGHPVGTGPYMLSK
WIPASRIVLKANPEYRGFIWNFNASSPGDEAIVKRLKGKQMPQIGTIDIQVMEENQSRWLAFQRGEVDIIQLEGQLVSKA
IKDGKLRPELAKEGVQLSRIVDPEISYIYWNLKDPVVGGMSKEKIALRRAIAMSRSIDQEIKLVRNSDAERLHFPVPPGV
VGYDPQYRSSTPYSVKAANLLLDRYHYKKDASGWRTQPNGKPLVVEYKARNDSIGQQSAELWKKNFDSLHIRMVYKPMLF
SDLLRSQKQCEGMFGSSAWIADYPDGDNFMQNFYGPNTHMTNWSCGSIPEFDELYRQSQQVKPGPERDALYRKMTRLLEV
YMPVQMSYARYRNMLAQPRIIGYKKHPILHAEWMYFDIDTNMKSNH

Sequences:

>Translated_606_residues
MTENLKQKLKHCSVGLFFAGMALTGSISIFAKSPADPNKVLRYVFPTAETGFDPAYVHDLYSAHVLTSIFETLYTYDYLA
RPAKLIPHVATAMPEVSADGLTYTIHIKKGIYFTADPAFKGKPRELTAYDYAYSFKRLLDPNLRSPNSWLLEDKIEGMNA
LVKAANKSGKFNYDQNVSGLQTPDKYTLVIRLVKPDYNFPLLLAHDPTGAVAREVIEKYKDKAGFVMGHPVGTGPYMLSK
WIPASRIVLKANPEYRGFIWNFNASSPGDEAIVKRLKGKQMPQIGTIDIQVMEENQSRWLAFQRGEVDIIQLEGQLVSKA
IKDGKLRPELAKEGVQLSRIVDPEISYIYWNLKDPVVGGMSKEKIALRRAIAMSRSIDQEIKLVRNSDAERLHFPVPPGV
VGYDPQYRSSTPYSVKAANLLLDRYHYKKDASGWRTQPNGKPLVVEYKARNDSIGQQSAELWKKNFDSLHIRMVYKPMLF
SDLLRSQKQCEGMFGSSAWIADYPDGDNFMQNFYGPNTHMTNWSCGSIPEFDELYRQSQQVKPGPERDALYRKMTRLLEV
YMPVQMSYARYRNMLAQPRIIGYKKHPILHAEWMYFDIDTNMKSNH
>Mature_605_residues
TENLKQKLKHCSVGLFFAGMALTGSISIFAKSPADPNKVLRYVFPTAETGFDPAYVHDLYSAHVLTSIFETLYTYDYLAR
PAKLIPHVATAMPEVSADGLTYTIHIKKGIYFTADPAFKGKPRELTAYDYAYSFKRLLDPNLRSPNSWLLEDKIEGMNAL
VKAANKSGKFNYDQNVSGLQTPDKYTLVIRLVKPDYNFPLLLAHDPTGAVAREVIEKYKDKAGFVMGHPVGTGPYMLSKW
IPASRIVLKANPEYRGFIWNFNASSPGDEAIVKRLKGKQMPQIGTIDIQVMEENQSRWLAFQRGEVDIIQLEGQLVSKAI
KDGKLRPELAKEGVQLSRIVDPEISYIYWNLKDPVVGGMSKEKIALRRAIAMSRSIDQEIKLVRNSDAERLHFPVPPGVV
GYDPQYRSSTPYSVKAANLLLDRYHYKKDASGWRTQPNGKPLVVEYKARNDSIGQQSAELWKKNFDSLHIRMVYKPMLFS
DLLRSQKQCEGMFGSSAWIADYPDGDNFMQNFYGPNTHMTNWSCGSIPEFDELYRQSQQVKPGPERDALYRKMTRLLEVY
MPVQMSYARYRNMLAQPRIIGYKKHPILHAEWMYFDIDTNMKSNH

Specific function: This protein is a component of the oligopeptide permease, a binding protein-dependent transport system, It binds peptides up to five amino acids long with high affinity. Also required for sporulation and competence [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787495, Length=605, Percent_Identity=24.297520661157, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI1789397, Length=572, Percent_Identity=22.3776223776224, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI87081878, Length=582, Percent_Identity=22.3367697594502, Blast_Score=72, Evalue=8e-14,
Organism=Escherichia coli, GI1787762, Length=228, Percent_Identity=24.5614035087719, Blast_Score=72, Evalue=9e-14,

Paralogues:

None

Copy number: 760 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2360 Molecules/Cell In: Stationary-Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 69115; Mature: 68984

Theoretical pI: Translated: 9.60; Mature: 9.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTENLKQKLKHCSVGLFFAGMALTGSISIFAKSPADPNKVLRYVFPTAETGFDPAYVHDL
CCHHHHHHHHHCCHHHHHHHHHHCCEEEEEECCCCCHHHHHEEEECCCCCCCCHHHHHHH
YSAHVLTSIFETLYTYDYLARPAKLIPHVATAMPEVSADGLTYTIHIKKGIYFTADPAFK
HHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCEEEEEEEECCEEEEECCCCC
GKPRELTAYDYAYSFKRLLDPNLRSPNSWLLEDKIEGMNALVKAANKSGKFNYDQNVSGL
CCCCEEEEHHHHHHHHHHCCCCCCCCCCEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
QTPDKYTLVIRLVKPDYNFPLLLAHDPTGAVAREVIEKYKDKAGFVMGHPVGTGPYMLSK
CCCCCEEEEEEEECCCCCCCEEEEECCCCHHHHHHHHHHHHCCCEEEECCCCCCCHHHHH
WIPASRIVLKANPEYRGFIWNFNASSPGDEAIVKRLKGKQMPQIGTIDIQVMEENQSRWL
CCCCCEEEEEECCCCEEEEEEECCCCCCHHHHHHHHCCCCCCCCCEEEEEEEECCCCEEE
AFQRGEVDIIQLEGQLVSKAIKDGKLRPELAKEGVQLSRIVDPEISYIYWNLKDPVVGGM
EEECCCEEEEEECHHHHHHHHHCCCCCHHHHHCCCEEEEECCCCEEEEEEECCCCCCCCC
SKEKIALRRAIAMSRSIDQEIKLVRNSDAERLHFPVPPGVVGYDPQYRSSTPYSVKAANL
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCCCEEHHHHH
LLDRYHYKKDASGWRTQPNGKPLVVEYKARNDSIGQQSAELWKKNFDSLHIRMVYKPMLF
HHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHCCCCCEEEEEEHHHHHH
SDLLRSQKQCEGMFGSSAWIADYPDGDNFMQNFYGPNTHMTNWSCGSIPEFDELYRQSQQ
HHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHC
VKPGPERDALYRKMTRLLEVYMPVQMSYARYRNMLAQPRIIGYKKHPILHAEWMYFDIDT
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCEEEEEEEEEEECC
NMKSNH
CCCCCC
>Mature Secondary Structure 
TENLKQKLKHCSVGLFFAGMALTGSISIFAKSPADPNKVLRYVFPTAETGFDPAYVHDL
CHHHHHHHHHCCHHHHHHHHHHCCEEEEEECCCCCHHHHHEEEECCCCCCCCHHHHHHH
YSAHVLTSIFETLYTYDYLARPAKLIPHVATAMPEVSADGLTYTIHIKKGIYFTADPAFK
HHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCEEEEEEEECCEEEEECCCCC
GKPRELTAYDYAYSFKRLLDPNLRSPNSWLLEDKIEGMNALVKAANKSGKFNYDQNVSGL
CCCCEEEEHHHHHHHHHHCCCCCCCCCCEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
QTPDKYTLVIRLVKPDYNFPLLLAHDPTGAVAREVIEKYKDKAGFVMGHPVGTGPYMLSK
CCCCCEEEEEEEECCCCCCCEEEEECCCCHHHHHHHHHHHHCCCEEEECCCCCCCHHHHH
WIPASRIVLKANPEYRGFIWNFNASSPGDEAIVKRLKGKQMPQIGTIDIQVMEENQSRWL
CCCCCEEEEEECCCCEEEEEEECCCCCCHHHHHHHHCCCCCCCCCEEEEEEEECCCCEEE
AFQRGEVDIIQLEGQLVSKAIKDGKLRPELAKEGVQLSRIVDPEISYIYWNLKDPVVGGM
EEECCCEEEEEECHHHHHHHHHCCCCCHHHHHCCCEEEEECCCCEEEEEEECCCCCCCCC
SKEKIALRRAIAMSRSIDQEIKLVRNSDAERLHFPVPPGVVGYDPQYRSSTPYSVKAANL
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCCCEEHHHHH
LLDRYHYKKDASGWRTQPNGKPLVVEYKARNDSIGQQSAELWKKNFDSLHIRMVYKPMLF
HHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHCCCCCEEEEEEHHHHHH
SDLLRSQKQCEGMFGSSAWIADYPDGDNFMQNFYGPNTHMTNWSCGSIPEFDELYRQSQQ
HHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHC
VKPGPERDALYRKMTRLLEVYMPVQMSYARYRNMLAQPRIIGYKKHPILHAEWMYFDIDT
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCEEEEEEEEEEECC
NMKSNH
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; oligopeptides [Periplasm]; H2O [C]

Specific reaction: ATP + oligopeptides [Periplasm] + H2O = ADP + phosphate + oligopeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1901616; 1899858; 9384377 [H]