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The map label for this gene is gltJ [H]

Identifier: 213157046

GI number: 213157046

Start: 1816632

End: 1817474

Strand: Direct

Name: gltJ [H]

Synonym: AB57_1726

Alternate gene names: 213157046

Gene position: 1816632-1817474 (Clockwise)

Preceding gene: 213157045

Following gene: 213157047

Centisome position: 44.85

GC content: 41.16

Gene sequence:

>843_bases
ATGTCAGTCGGCTCACTAAACTGGACTATATTCTGTGCGGAGTCTAATGAATATGGTGTCGATAAAGCCGCATGGGCAGA
ATCAGTTTGTAAGGTTATTGGTAGTTCTAGCTACAATGCCTATGCTGAGGCGCCCACATGGTTACAAATGTTAGGTTCTG
GTGTCTTTACTATGGTTTGGACTGCCATTGTAGCCTTTCTTATCGCATTTTTATTAGGTTCTCTCCTCGGGGTAATTCGT
ACTTTACCCAATAAACCACTTGCTTTTATCGGCAACTGTTATGTCGAAATATTCCGTAATATTCCATTAATTGTACAGTT
ATTCTTCTGGGCTTTTGTTTTTCCGGAGTTCTTACCGGACAGTTTAAGTGCAGGAAGTGGAGATGTTGTAGCAGGGGGAT
GGTGGAAAAACATTTTAAATCAACAACCCGCTGTTATCGGTGTTTTTGCTTTAGGCCTTTATACTGCGGCGCGTGTTTCG
GAGCATGTCAGAGCCGGAATTAACACGGTTTCTCGTGGTCAAAAGTTTGCGGCATCAGCTATGGGATTTACCACCGCTCA
AAGTTATCGTTATGTGATTTTGCCAGTTGCGTACCGTACGGTATGGCCAACGATTACGTCAGAAGCAATGAACGTCTTTA
AAAACTCGGCAGTTTTATATGCCTTAAGTGTTTTAAATTTCTTTGCTTATACCAAAACCATGCGTGAAGAGACTTCACAG
GACATTATCATTCTTATTCTGTCTACACCTGTCTATTTAATCATTACATACACCATTAAATTTATAATGGCATGGATTGA
AAAACGCATGGCTGTACCAGGTTTAGGCTCGGGAGGAAAATAA

Upstream 100 bases:

>100_bases
ATCTAACTGGATCTTTTTGTCCTAAATTTACTTGTGCAGGTGTAAAAATTCATTTGCACAAGTGGTCTATTTTCTAAAGA
TATTAATTTAAGGGGCAAAC

Downstream 100 bases:

>100_bases
TTATGGATTTTTCTCTATTGCATAATCCTGATGTTATTGCGGCATTAAAAGAAGGATTTATTTTTACACTTACCGTAACC
GTGCTTGCAATGATGGGGGG

Product: glutamate/aspartate transport system permease protein GltJ

Products: ADP; phosphate; L-glutamate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 280; Mature: 279

Protein sequence:

>280_residues
MSVGSLNWTIFCAESNEYGVDKAAWAESVCKVIGSSSYNAYAEAPTWLQMLGSGVFTMVWTAIVAFLIAFLLGSLLGVIR
TLPNKPLAFIGNCYVEIFRNIPLIVQLFFWAFVFPEFLPDSLSAGSGDVVAGGWWKNILNQQPAVIGVFALGLYTAARVS
EHVRAGINTVSRGQKFAASAMGFTTAQSYRYVILPVAYRTVWPTITSEAMNVFKNSAVLYALSVLNFFAYTKTMREETSQ
DIIILILSTPVYLIITYTIKFIMAWIEKRMAVPGLGSGGK

Sequences:

>Translated_280_residues
MSVGSLNWTIFCAESNEYGVDKAAWAESVCKVIGSSSYNAYAEAPTWLQMLGSGVFTMVWTAIVAFLIAFLLGSLLGVIR
TLPNKPLAFIGNCYVEIFRNIPLIVQLFFWAFVFPEFLPDSLSAGSGDVVAGGWWKNILNQQPAVIGVFALGLYTAARVS
EHVRAGINTVSRGQKFAASAMGFTTAQSYRYVILPVAYRTVWPTITSEAMNVFKNSAVLYALSVLNFFAYTKTMREETSQ
DIIILILSTPVYLIITYTIKFIMAWIEKRMAVPGLGSGGK
>Mature_279_residues
SVGSLNWTIFCAESNEYGVDKAAWAESVCKVIGSSSYNAYAEAPTWLQMLGSGVFTMVWTAIVAFLIAFLLGSLLGVIRT
LPNKPLAFIGNCYVEIFRNIPLIVQLFFWAFVFPEFLPDSLSAGSGDVVAGGWWKNILNQQPAVIGVFALGLYTAARVSE
HVRAGINTVSRGQKFAASAMGFTTAQSYRYVILPVAYRTVWPTITSEAMNVFKNSAVLYALSVLNFFAYTKTMREETSQD
IIILILSTPVYLIITYTIKFIMAWIEKRMAVPGLGSGGK

Specific function: Part of the binding-protein-dependent transport system for aspartate/glutamate; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0765

COG function: function code E; ABC-type amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1786874, Length=237, Percent_Identity=41.3502109704641, Blast_Score=169, Evalue=2e-43,
Organism=Escherichia coli, GI1786873, Length=224, Percent_Identity=29.4642857142857, Blast_Score=111, Evalue=5e-26,
Organism=Escherichia coli, GI48994930, Length=125, Percent_Identity=35.2, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1788226, Length=201, Percent_Identity=24.8756218905473, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1788645, Length=218, Percent_Identity=27.5229357798165, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1787087, Length=226, Percent_Identity=23.8938053097345, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1787030, Length=226, Percent_Identity=23.0088495575221, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787086, Length=225, Percent_Identity=21.3333333333333, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010065
- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30775; Mature: 30644

Theoretical pI: Translated: 8.99; Mature: 8.99

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVGSLNWTIFCAESNEYGVDKAAWAESVCKVIGSSSYNAYAEAPTWLQMLGSGVFTMVW
CCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCHHHHHH
TAIVAFLIAFLLGSLLGVIRTLPNKPLAFIGNCYVEIFRNIPLIVQLFFWAFVFPEFLPD
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCC
SLSAGSGDVVAGGWWKNILNQQPAVIGVFALGLYTAARVSEHVRAGINTVSRGQKFAASA
CCCCCCCCEEECHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MGFTTAQSYRYVILPVAYRTVWPTITSEAMNVFKNSAVLYALSVLNFFAYTKTMREETSQ
HHHHHHCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
DIIILILSTPVYLIITYTIKFIMAWIEKRMAVPGLGSGGK
CEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SVGSLNWTIFCAESNEYGVDKAAWAESVCKVIGSSSYNAYAEAPTWLQMLGSGVFTMVW
CCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCHHHHHH
TAIVAFLIAFLLGSLLGVIRTLPNKPLAFIGNCYVEIFRNIPLIVQLFFWAFVFPEFLPD
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCC
SLSAGSGDVVAGGWWKNILNQQPAVIGVFALGLYTAARVSEHVRAGINTVSRGQKFAASA
CCCCCCCCEEECHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MGFTTAQSYRYVILPVAYRTVWPTITSEAMNVFKNSAVLYALSVLNFFAYTKTMREETSQ
HHHHHHCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
DIIILILSTPVYLIITYTIKFIMAWIEKRMAVPGLGSGGK
CEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamate [Periplasm]; H2O [C]

Specific reaction: ATP + L-glutamate [Periplasm] + H2O = ADP + phosphate + L-glutamate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]