Click here to switch to the map view.

The map label for this gene is pcaJ [H]

Identifier: 213156918

GI number: 213156918

Start: 1675135

End: 1675788

Strand: Direct

Name: pcaJ [H]

Synonym: AB57_1589

Alternate gene names: 213156918

Gene position: 1675135-1675788 (Clockwise)

Preceding gene: 213156917

Following gene: 213156919

Centisome position: 41.36

GC content: 42.51

Gene sequence:

>654_bases
ATGTCAGTTCAAAAGCGTTCTCGTGAAGATATTGCCAAGCTCATTGCCAATGACATTCCGGAAGGTTCTTATGTCAATTT
AGGCATTGGTTTACCGACCAACGTTGCTAAATTTTTGCCAAAAGACAAAGAAATCTTTTTACATTCGGAAAATGGGGTTT
TGGCTTTTGGTCCACCACCACAACCTGGTGAGGAAGATCAAGATTTGGTTAATGCCGGAAAAGAACTTGTTACTTTGTTA
GACGGCGGATGCTTTATGCATCATGGCGACTCATTTGACATTATGCGTGGCGGCCATTTGGACATTTGTGTGATCGGTGC
ATTCCAAGTTGCGGTAAATGGAGACTTGGCAAACTGGCATACCGGTAAGGCAGATGATGTGCCGGCAGTTGGCGGTGCTA
TGGATTTGGCAGTCGGTGCAAAAACGATTTATGTCTATATGGAGCATGTCACTAAAAAAGGTGAGCCCAAAATTGTAAAA
GAACTGAGCTATCCAATAACGGGTGAGCAGTGTGTAAACCGTATTTATACCGATTTATGCATTATTGAACTCAAAGACCA
AAAAGCTTACGTCAAGGAAATGGTAGACGGCTTAACTTTTGATGAATTACAGGCAGTCACTGACTGTGAACTAATCGATG
CACGTGTAGCCTAA

Upstream 100 bases:

>100_bases
TTCAAGTGAATGACACCGTAGAGCTAGGAACGCTTGACCCAGAGTGCATTATTACACCAGGAATTTTTGTTCAGCACGTT
GTAAAAGTAGGAGATATCTA

Downstream 100 bases:

>100_bases
ATACACACGGAAAAACGCTTCTTTAAGGATTAAAAGATGAAAAAATTAATTTTAGCTGCAGCTTGTGCTGCGGCTTCAGG
GACTATTTTTGCAAATTCTT

Product: 3-oxoadipate CoA-transferase subunit B

Products: NA

Alternate protein names: Beta-ketoadipate:succinyl-CoA transferase subunit B [H]

Number of amino acids: Translated: 217; Mature: 216

Protein sequence:

>217_residues
MSVQKRSREDIAKLIANDIPEGSYVNLGIGLPTNVAKFLPKDKEIFLHSENGVLAFGPPPQPGEEDQDLVNAGKELVTLL
DGGCFMHHGDSFDIMRGGHLDICVIGAFQVAVNGDLANWHTGKADDVPAVGGAMDLAVGAKTIYVYMEHVTKKGEPKIVK
ELSYPITGEQCVNRIYTDLCIIELKDQKAYVKEMVDGLTFDELQAVTDCELIDARVA

Sequences:

>Translated_217_residues
MSVQKRSREDIAKLIANDIPEGSYVNLGIGLPTNVAKFLPKDKEIFLHSENGVLAFGPPPQPGEEDQDLVNAGKELVTLL
DGGCFMHHGDSFDIMRGGHLDICVIGAFQVAVNGDLANWHTGKADDVPAVGGAMDLAVGAKTIYVYMEHVTKKGEPKIVK
ELSYPITGEQCVNRIYTDLCIIELKDQKAYVKEMVDGLTFDELQAVTDCELIDARVA
>Mature_216_residues
SVQKRSREDIAKLIANDIPEGSYVNLGIGLPTNVAKFLPKDKEIFLHSENGVLAFGPPPQPGEEDQDLVNAGKELVTLLD
GGCFMHHGDSFDIMRGGHLDICVIGAFQVAVNGDLANWHTGKADDVPAVGGAMDLAVGAKTIYVYMEHVTKKGEPKIVKE
LSYPITGEQCVNRIYTDLCIIELKDQKAYVKEMVDGLTFDELQAVTDCELIDARVA

Specific function: SHORT-CHAIN FATTY ACIDS METABOLISM. [C]

COG id: COG2057

COG function: function code I; Acyl CoA:acetate/3-ketoacid CoA transferase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-oxoacid CoA-transferase subunit B family [H]

Homologues:

Organism=Homo sapiens, GI11545841, Length=207, Percent_Identity=42.0289855072464, Blast_Score=173, Evalue=9e-44,
Organism=Homo sapiens, GI4557817, Length=207, Percent_Identity=44.9275362318841, Blast_Score=172, Evalue=3e-43,
Organism=Escherichia coli, GI1788552, Length=206, Percent_Identity=38.8349514563107, Blast_Score=152, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI17531517, Length=214, Percent_Identity=43.4579439252336, Blast_Score=167, Evalue=3e-42,
Organism=Drosophila melanogaster, GI21358325, Length=207, Percent_Identity=39.6135265700483, Blast_Score=152, Evalue=2e-37,
Organism=Drosophila melanogaster, GI24655794, Length=207, Percent_Identity=39.6135265700483, Blast_Score=152, Evalue=2e-37,

Paralogues:

None

Copy number: 369 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012791
- InterPro:   IPR004165
- InterPro:   IPR004164 [H]

Pfam domain/function: PF01144 CoA_trans [H]

EC number: =2.8.3.6 [H]

Molecular weight: Translated: 23616; Mature: 23485

Theoretical pI: Translated: 4.51; Mature: 4.51

Prosite motif: PS01274 COA_TRANSF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVQKRSREDIAKLIANDIPEGSYVNLGIGLPTNVAKFLPKDKEIFLHSENGVLAFGPPP
CCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHCCCCCEEEEECCCCEEEECCCC
QPGEEDQDLVNAGKELVTLLDGGCFMHHGDSFDIMRGGHLDICVIGAFQVAVNGDLANWH
CCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCEEECCCEEEEEEEEEEEEECCCCCCCC
TGKADDVPAVGGAMDLAVGAKTIYVYMEHVTKKGEPKIVKELSYPITGEQCVNRIYTDLC
CCCCCCCCCCCCHHHHHHCHHHHHHEEHHHHCCCCCHHHHHCCCCCCHHHHHHHHHHHEE
IIELKDQKAYVKEMVDGLTFDELQAVTDCELIDARVA
EEEECCHHHHHHHHHCCCCHHHHHHHHCHHHHHCCCC
>Mature Secondary Structure 
SVQKRSREDIAKLIANDIPEGSYVNLGIGLPTNVAKFLPKDKEIFLHSENGVLAFGPPP
CCCHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHCCCCCEEEEECCCCEEEECCCC
QPGEEDQDLVNAGKELVTLLDGGCFMHHGDSFDIMRGGHLDICVIGAFQVAVNGDLANWH
CCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCEEECCCEEEEEEEEEEEEECCCCCCCC
TGKADDVPAVGGAMDLAVGAKTIYVYMEHVTKKGEPKIVKELSYPITGEQCVNRIYTDLC
CCCCCCCCCCCCHHHHHHCHHHHHHEEHHHHCCCCCHHHHHCCCCCCHHHHHHHHHHHEE
IIELKDQKAYVKEMVDGLTFDELQAVTDCELIDARVA
EEEECCHHHHHHHHHCCCCHHHHHHHHCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8063101; 8125318 [H]