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The map label for this gene is rep [H]

Identifier: 21244998

GI number: 21244998

Start: 5056650

End: 5058626

Strand: Reverse

Name: rep [H]

Synonym: XAC4281

Alternate gene names: 21244998

Gene position: 5058626-5056650 (Counterclockwise)

Preceding gene: 21245001

Following gene: 21244997

Centisome position: 97.74

GC content: 63.58

Gene sequence:

>1977_bases
ATGTACGGTCTCAATCCCCCGCAAAGCGCCGCGGTGCTGCACTGCGAAGGTCCCTTGTTGGTGCTGGCCGGTGCCGGCAG
CGGCAAGACGCGTGTGATCGTGGAAAAGATCGCGCATCTGATCGCCATCGGCCGCTATCCGGCCAAGCGGATCGCCGCGA
TCACCTTCACCAACAAATCCGCCAAGGAAATGCGCGAGCGTGTGGCCAAACGCATCCGCGGCGACGGTGCTGAAGGCCTG
ACCATCTGCACCTTCCACGCGCTGGGCCTGAAGTTCCTGCAGATCGAGCATGCCGCTGCCGGTTTGAAGCGCGGCTTTTC
GATCTTCGATTCGGACGATGCCGCTGCGCAGATCAAGGACCTGATGCATGGCGCCAAGCCCGATGCGATCGAAGACGCCA
AGAACCTGATTTCGCGCGCCAAGAACGCCGGCCTGTCGCCGGAACAGGCGATGGCAGCTGCGCGCAGCACCCGCGAAAAG
GAAGCGGCCAGCTTGTACGAGCGCTACCAGGCGCGGCTGACCACCTTCAACGCGGTGGATTTCGATGACCTGATCCGCCT
GCCGGTGCAGATCCTGGAAGCCAACGAAGACATCGTGATGGGCTGGCGCGAGCGCATCGGCTACCTGCTGGTGGACGAGT
GCCAGGACACCAACGATGCGCAGTACCGGCTGTTGAAGATGCTGGCCGGCCCGCGCGGCAACTTCACCTGCGTGGGCGAC
GACGACCAGAGCATCTATGCCTGGCGCGGCGCCAATCCGGAAAACCTGCAGCAGATGGCGCGCGACTATCCGGCGCTGGA
AATCATCAAGCTGGAACAGAACTACCGCTGCTCCAATCGCGTGCTGCGCGCGGCCAACGCGTTGATCGCGCATAACCCGC
ACGAACACTTGAAGACCTTGTGGAGCGACCAGGCCGACGGCGAGCGCATCCGCGTGTGGGAATGCCGCGACAGCGAGCAC
GAGGCCGAAAAGGTCGCGGCGGAGATCTCGTTCCTGGGCACCGCCAAGCAGGTGCCGTGGAGCGATTTCTGCATTCTGTT
CCGCGGCAATTTTCAGTCGCGTCCGCTGGAAAAAGCGCTGCAGCTGCTGCGCGTGCCATATCACCTCACCGGTGGCACCG
CGTTTCTGGAGCGGCAGGAAGTCAAGGATCTGCTGTCGTGGTTGCGGTTGATCGTCAACCCGGACGACGACGCGGCGTTC
TTGCGTGCCGTGCAATCGCCCAAGCGCGAAGTCGGCGCGACCTCGCTGGCGCGTCTGGCCGAGCTTGCCAGCGCCAAGTC
GGTGCCGATGTCGCGCGCAGCCGAGTCGATGGGCGCCCTGCAGCATTTGCCGCCGCGCGCGGCCAACGGTTTGAGTGCGT
TTACCGACATCCTGCGCGACATGCGGGAGCACTCGTCCAGCATGCCGGCCGGCGAGCTGGTGCGCCTGCTGGCCGACAAG
TCCGGTTTGCTCAACGACCTGCGCAATCAATCTAAAGACGAGACCGGCTTCCAGCGGCGCAAGCGCAATCTGGACGAACT
GGCCGAGTGGTTCGAAGGTGGTCCGCGCGGTGCAAGCGCCAGCGATCTGGCTGCGCAGTTGGCGTTGCTGTCGCGCAACG
ACAAGGACGATGGCGGCAACCAGGTGCGCATGATGACCATGCATGCGTCCAAGGGACTGGAATTCCGCTATGTCTTCATC
GTCGGCTGCGAGGACGGCGTACTGCCGCACGAAGTGAGCCTGGAAGAAGGTAATCTGCAGGAAGAGCGTCGCCTGCTGTA
CGTGGGCATCACCCGCGCCAAGGAGCAGTTGTGGATGAGTCACAGCAAGCTGACGCGCAAGTTCGGTGAGCATGTGCGAC
TGAAGCCGAGCCGGTTCTTCGATGAGATTCCGGCCGAAGAACTGCAGCGCGATGGCGCCGACCCGGTGGCCGACGCCGAG
CGCAAGAAAGAACGCGCCAGTGCGGGATTGGCCGCGATCCAGGCATTGTTCGACTGA

Upstream 100 bases:

>100_bases
TGGCTTGAGGCAAGGGCTAGCGGTGCTGGAGACATGAATCCATCGCGCCGGCCAGCCGCAAGCAGCCCCGGTACAATGGC
GCGCTCTGTCGGAAGCCACT

Downstream 100 bases:

>100_bases
TGCGCAGTGCGCCGTAGCACGCGCACCTGCCGCTCATCGCTTGCCAGTGACGCGCCGGCACGGCAGTCTGGTTTTTCATG
ATGCCGGAGTGTGCCGTGAC

Product: ATP-dependent DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 658; Mature: 658

Protein sequence:

>658_residues
MYGLNPPQSAAVLHCEGPLLVLAGAGSGKTRVIVEKIAHLIAIGRYPAKRIAAITFTNKSAKEMRERVAKRIRGDGAEGL
TICTFHALGLKFLQIEHAAAGLKRGFSIFDSDDAAAQIKDLMHGAKPDAIEDAKNLISRAKNAGLSPEQAMAAARSTREK
EAASLYERYQARLTTFNAVDFDDLIRLPVQILEANEDIVMGWRERIGYLLVDECQDTNDAQYRLLKMLAGPRGNFTCVGD
DDQSIYAWRGANPENLQQMARDYPALEIIKLEQNYRCSNRVLRAANALIAHNPHEHLKTLWSDQADGERIRVWECRDSEH
EAEKVAAEISFLGTAKQVPWSDFCILFRGNFQSRPLEKALQLLRVPYHLTGGTAFLERQEVKDLLSWLRLIVNPDDDAAF
LRAVQSPKREVGATSLARLAELASAKSVPMSRAAESMGALQHLPPRAANGLSAFTDILRDMREHSSSMPAGELVRLLADK
SGLLNDLRNQSKDETGFQRRKRNLDELAEWFEGGPRGASASDLAAQLALLSRNDKDDGGNQVRMMTMHASKGLEFRYVFI
VGCEDGVLPHEVSLEEGNLQEERRLLYVGITRAKEQLWMSHSKLTRKFGEHVRLKPSRFFDEIPAEELQRDGADPVADAE
RKKERASAGLAAIQALFD

Sequences:

>Translated_658_residues
MYGLNPPQSAAVLHCEGPLLVLAGAGSGKTRVIVEKIAHLIAIGRYPAKRIAAITFTNKSAKEMRERVAKRIRGDGAEGL
TICTFHALGLKFLQIEHAAAGLKRGFSIFDSDDAAAQIKDLMHGAKPDAIEDAKNLISRAKNAGLSPEQAMAAARSTREK
EAASLYERYQARLTTFNAVDFDDLIRLPVQILEANEDIVMGWRERIGYLLVDECQDTNDAQYRLLKMLAGPRGNFTCVGD
DDQSIYAWRGANPENLQQMARDYPALEIIKLEQNYRCSNRVLRAANALIAHNPHEHLKTLWSDQADGERIRVWECRDSEH
EAEKVAAEISFLGTAKQVPWSDFCILFRGNFQSRPLEKALQLLRVPYHLTGGTAFLERQEVKDLLSWLRLIVNPDDDAAF
LRAVQSPKREVGATSLARLAELASAKSVPMSRAAESMGALQHLPPRAANGLSAFTDILRDMREHSSSMPAGELVRLLADK
SGLLNDLRNQSKDETGFQRRKRNLDELAEWFEGGPRGASASDLAAQLALLSRNDKDDGGNQVRMMTMHASKGLEFRYVFI
VGCEDGVLPHEVSLEEGNLQEERRLLYVGITRAKEQLWMSHSKLTRKFGEHVRLKPSRFFDEIPAEELQRDGADPVADAE
RKKERASAGLAAIQALFD
>Mature_658_residues
MYGLNPPQSAAVLHCEGPLLVLAGAGSGKTRVIVEKIAHLIAIGRYPAKRIAAITFTNKSAKEMRERVAKRIRGDGAEGL
TICTFHALGLKFLQIEHAAAGLKRGFSIFDSDDAAAQIKDLMHGAKPDAIEDAKNLISRAKNAGLSPEQAMAAARSTREK
EAASLYERYQARLTTFNAVDFDDLIRLPVQILEANEDIVMGWRERIGYLLVDECQDTNDAQYRLLKMLAGPRGNFTCVGD
DDQSIYAWRGANPENLQQMARDYPALEIIKLEQNYRCSNRVLRAANALIAHNPHEHLKTLWSDQADGERIRVWECRDSEH
EAEKVAAEISFLGTAKQVPWSDFCILFRGNFQSRPLEKALQLLRVPYHLTGGTAFLERQEVKDLLSWLRLIVNPDDDAAF
LRAVQSPKREVGATSLARLAELASAKSVPMSRAAESMGALQHLPPRAANGLSAFTDILRDMREHSSSMPAGELVRLLADK
SGLLNDLRNQSKDETGFQRRKRNLDELAEWFEGGPRGASASDLAAQLALLSRNDKDDGGNQVRMMTMHASKGLEFRYVFI
VGCEDGVLPHEVSLEEGNLQEERRLLYVGITRAKEQLWMSHSKLTRKFGEHVRLKPSRFFDEIPAEELQRDGADPVADAE
RKKERASAGLAAIQALFD

Specific function: Rep helicase is a single-stranded DNA-dependent ATPase involved in DNA replication; it can initiate unwinding at a nick in the DNA. It binds to the single-stranded DNA and acts in a progressive fashion along the DNA in the 3' to 5' direction [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI48994965, Length=670, Percent_Identity=44.6268656716418, Blast_Score=536, Evalue=1e-153,
Organism=Escherichia coli, GI2367296, Length=642, Percent_Identity=38.4735202492212, Blast_Score=393, Evalue=1e-110,
Organism=Escherichia coli, GI1787196, Length=342, Percent_Identity=30.7017543859649, Blast_Score=105, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6322369, Length=732, Percent_Identity=25.8196721311475, Blast_Score=185, Evalue=2e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005752
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 73243; Mature: 73243

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYGLNPPQSAAVLHCEGPLLVLAGAGSGKTRVIVEKIAHLIAIGRYPAKRIAAITFTNKS
CCCCCCCCCCEEEEECCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCHHHEEEEEECCCH
AKEMRERVAKRIRGDGAEGLTICTFHALGLKFLQIEHAAAGLKRGFSIFDSDDAAAQIKD
HHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
LMHGAKPDAIEDAKNLISRAKNAGLSPEQAMAAARSTREKEAASLYERYQARLTTFNAVD
HHCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
FDDLIRLPVQILEANEDIVMGWRERIGYLLVDECQDTNDAQYRLLKMLAGPRGNFTCVGD
HHHHHHHHHHHHHCCCHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHCCCCCEEEECC
DDQSIYAWRGANPENLQQMARDYPALEIIKLEQNYRCSNRVLRAANALIAHNPHEHLKTL
CCCCEEEECCCCHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCHHHHHHH
WSDQADGERIRVWECRDSEHEAEKVAAEISFLGTAKQVPWSDFCILFRGNFQSRPLEKAL
HCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCHHHHH
QLLRVPYHLTGGTAFLERQEVKDLLSWLRLIVNPDDDAAFLRAVQSPKREVGATSLARLA
HHHHCCCEECCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCHHHHHHHHHHHHHH
ELASAKSVPMSRAAESMGALQHLPPRAANGLSAFTDILRDMREHSSSMPAGELVRLLADK
HHHHHCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHC
SGLLNDLRNQSKDETGFQRRKRNLDELAEWFEGGPRGASASDLAAQLALLSRNDKDDGGN
HHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCC
QVRMMTMHASKGLEFRYVFIVGCEDGVLPHEVSLEEGNLQEERRLLYVGITRAKEQLWMS
EEEEEEEECCCCCEEEEEEEEECCCCCCCCCCCCCCCCCHHHCEEEEEEHHHHHHHHHHH
HSKLTRKFGEHVRLKPSRFFDEIPAEELQRDGADPVADAERKKERASAGLAAIQALFD
HHHHHHHHHHCEECCHHHHHHCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MYGLNPPQSAAVLHCEGPLLVLAGAGSGKTRVIVEKIAHLIAIGRYPAKRIAAITFTNKS
CCCCCCCCCCEEEEECCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCHHHEEEEEECCCH
AKEMRERVAKRIRGDGAEGLTICTFHALGLKFLQIEHAAAGLKRGFSIFDSDDAAAQIKD
HHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
LMHGAKPDAIEDAKNLISRAKNAGLSPEQAMAAARSTREKEAASLYERYQARLTTFNAVD
HHCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
FDDLIRLPVQILEANEDIVMGWRERIGYLLVDECQDTNDAQYRLLKMLAGPRGNFTCVGD
HHHHHHHHHHHHHCCCHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHCCCCCEEEECC
DDQSIYAWRGANPENLQQMARDYPALEIIKLEQNYRCSNRVLRAANALIAHNPHEHLKTL
CCCCEEEECCCCHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCHHHHHHH
WSDQADGERIRVWECRDSEHEAEKVAAEISFLGTAKQVPWSDFCILFRGNFQSRPLEKAL
HCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCHHHHH
QLLRVPYHLTGGTAFLERQEVKDLLSWLRLIVNPDDDAAFLRAVQSPKREVGATSLARLA
HHHHCCCEECCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCHHHHHHHHHHHHHH
ELASAKSVPMSRAAESMGALQHLPPRAANGLSAFTDILRDMREHSSSMPAGELVRLLADK
HHHHHCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHC
SGLLNDLRNQSKDETGFQRRKRNLDELAEWFEGGPRGASASDLAAQLALLSRNDKDDGGN
HHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCC
QVRMMTMHASKGLEFRYVFIVGCEDGVLPHEVSLEEGNLQEERRLLYVGITRAKEQLWMS
EEEEEEEECCCCCEEEEEEEEECCCCCCCCCCCCCCCCCHHHCEEEEEEHHHHHHHHHHH
HSKLTRKFGEHVRLKPSRFFDEIPAEELQRDGADPVADAERKKERASAGLAAIQALFD
HHHHHHHHHHCEECCHHHHHHCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]