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The map label for this gene is trpS

Identifier: 21244724

GI number: 21244724

Start: 4698692

End: 4699990

Strand: Reverse

Name: trpS

Synonym: XAC4006

Alternate gene names: 21244724

Gene position: 4699990-4698692 (Counterclockwise)

Preceding gene: 21244725

Following gene: 21244723

Centisome position: 90.81

GC content: 63.59

Gene sequence:

>1299_bases
ATGACGTCGATGACCACCCGTGTCCTTACCGGCATCACCACCTCCGGCACCCCGCATCTGGGCAATTACGTGGGTGCGAT
CCGCCCGGCCATCCAGGCCAGTGCAGGCGCCGATGCGGAGAGCTTCTATTTCCTGGCCGATCTGCATAGCCTGATCAAGG
CGCAGGACCCGGCACGCACGCAGCGTTCGACACTGGAAATCGCCGCCACCTGGCTGGCCTGCGGCCTGGACCCGGACAAG
GTGTGGTTCTACCGGCAGTCCGATGTGCCCGAGACCACCGAGTTGATGTGGTTGCTGACCTGCGTGGCCGGCAAGGGCAT
CCTCAATCGCGCGCATGCCTACAAGGCGGCGGTGGACAGGAACCGCGCCGATGGCGAGGACGAGGATGCCGGCGTTACCG
CAGGCTTGTTCATGTATCCGGTGTTGATGGCGGCCGATATCTTGATCTTCAACGCGCACCAGGTGCCGGTCGGGCGCGAC
CAGATCCAGCACATCGAAATGGCACGCGATTTCGCGCAGCGTTTCAATCATGTCTATGGGCGCGAATTTTTCACCCTGCC
CGAAGCGGTGATCGACGAGCAGGTGTCCACCTTGCCGGGCCTGGATGGCCGCAAGATGAGCAAGAGCTATGGCAATACCA
TTCCACTGTTCGCACCGCGCGAGGAATTGCGCAAGCTGGTGTTTTCCATCCTCACCGATTCGCGCGCCCCGGGCCAAGCG
AAGGACACGCAGGGGTCGGCGTTGTTTCAGCTGTATCAGGCATTTGCCACGCCGCAGGAATCTGCCGCCTTTGCGCAGGC
GTTTGCCGACGGCATCGGTTGGGGCGATGCCAAGCAGCAGGTGTTCGAGCGGATCGATCAAGAGATCGCGCCGCTGCGTA
CGCGCTACGAGGGCTTGATTGCAGAGCCGGCCAGGATCGAAGCGATTCTGCGCGCCGGTGGTGCACGTCTGCGCGCGCGC
TACGCCACCCCGCTGCTGGCCGAGTTGCGCGACGCGGTGGGCCTGCGCGACCTGTCCAGCCAAGCCGCCACCGCGGCGGT
GCATGCCAGCGAAAAGATCGCACTGCCGGTGTTCAAGCAATATCGTGAAAGCGATGGCCAGTTCTACTTCAAGTTGAACG
ATGGCGCGGGTGCATTGCTGCTGCAGAGCGATGGCTTCGCTTCGCCACGCGATGCCGGACAGGTGATCGCGCGGCTGAAA
CAGGCCGCGCAGGCCAGCGATTTGCAGCTGCCGGGCGTGCATGCGCAAGTGGATGCCGACGTGGTTCTTGCTGCGATGGA
CGCCTTGCGCGAGGCGTGA

Upstream 100 bases:

>100_bases
CGTTGGAGCGTCGAACCAACTCCCGGGCCTCTGCAGCGGTGGCAAAAGTGCGGAACCGCTAAACTGGAACCGCTAAACTT
GGGGGCTACCCCATCCTGGC

Downstream 100 bases:

>100_bases
CCTGCGCTGCGCAGCAGGCGTAGAGGTGGACGATCGCGGTGCGTAAGCTCGTGCCTGTGGCGACATGCACGACAGCGACG
CCAGGCCTGGGCCTACACTG

Product: tryptophanyl-tRNA synthetase

Products: NA

Alternate protein names: Tryptophan--tRNA ligase; TrpRS

Number of amino acids: Translated: 432; Mature: 431

Protein sequence:

>432_residues
MTSMTTRVLTGITTSGTPHLGNYVGAIRPAIQASAGADAESFYFLADLHSLIKAQDPARTQRSTLEIAATWLACGLDPDK
VWFYRQSDVPETTELMWLLTCVAGKGILNRAHAYKAAVDRNRADGEDEDAGVTAGLFMYPVLMAADILIFNAHQVPVGRD
QIQHIEMARDFAQRFNHVYGREFFTLPEAVIDEQVSTLPGLDGRKMSKSYGNTIPLFAPREELRKLVFSILTDSRAPGQA
KDTQGSALFQLYQAFATPQESAAFAQAFADGIGWGDAKQQVFERIDQEIAPLRTRYEGLIAEPARIEAILRAGGARLRAR
YATPLLAELRDAVGLRDLSSQAATAAVHASEKIALPVFKQYRESDGQFYFKLNDGAGALLLQSDGFASPRDAGQVIARLK
QAAQASDLQLPGVHAQVDADVVLAAMDALREA

Sequences:

>Translated_432_residues
MTSMTTRVLTGITTSGTPHLGNYVGAIRPAIQASAGADAESFYFLADLHSLIKAQDPARTQRSTLEIAATWLACGLDPDK
VWFYRQSDVPETTELMWLLTCVAGKGILNRAHAYKAAVDRNRADGEDEDAGVTAGLFMYPVLMAADILIFNAHQVPVGRD
QIQHIEMARDFAQRFNHVYGREFFTLPEAVIDEQVSTLPGLDGRKMSKSYGNTIPLFAPREELRKLVFSILTDSRAPGQA
KDTQGSALFQLYQAFATPQESAAFAQAFADGIGWGDAKQQVFERIDQEIAPLRTRYEGLIAEPARIEAILRAGGARLRAR
YATPLLAELRDAVGLRDLSSQAATAAVHASEKIALPVFKQYRESDGQFYFKLNDGAGALLLQSDGFASPRDAGQVIARLK
QAAQASDLQLPGVHAQVDADVVLAAMDALREA
>Mature_431_residues
TSMTTRVLTGITTSGTPHLGNYVGAIRPAIQASAGADAESFYFLADLHSLIKAQDPARTQRSTLEIAATWLACGLDPDKV
WFYRQSDVPETTELMWLLTCVAGKGILNRAHAYKAAVDRNRADGEDEDAGVTAGLFMYPVLMAADILIFNAHQVPVGRDQ
IQHIEMARDFAQRFNHVYGREFFTLPEAVIDEQVSTLPGLDGRKMSKSYGNTIPLFAPREELRKLVFSILTDSRAPGQAK
DTQGSALFQLYQAFATPQESAAFAQAFADGIGWGDAKQQVFERIDQEIAPLRTRYEGLIAEPARIEAILRAGGARLRARY
ATPLLAELRDAVGLRDLSSQAATAAVHASEKIALPVFKQYRESDGQFYFKLNDGAGALLLQSDGFASPRDAGQVIARLKQ
AAQASDLQLPGVHAQVDADVVLAAMDALREA

Specific function: Unknown

COG id: COG0180

COG function: function code J; Tryptophanyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI7710154, Length=336, Percent_Identity=33.0357142857143, Blast_Score=168, Evalue=7e-42,
Organism=Homo sapiens, GI41352700, Length=189, Percent_Identity=42.8571428571429, Blast_Score=163, Evalue=2e-40,
Organism=Escherichia coli, GI1789786, Length=338, Percent_Identity=33.7278106508876, Blast_Score=164, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI71982800, Length=355, Percent_Identity=30.7042253521127, Blast_Score=149, Evalue=4e-36,
Organism=Caenorhabditis elegans, GI71982793, Length=355, Percent_Identity=30.7042253521127, Blast_Score=147, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6320474, Length=344, Percent_Identity=31.1046511627907, Blast_Score=144, Evalue=4e-35,
Organism=Drosophila melanogaster, GI24666151, Length=360, Percent_Identity=30, Blast_Score=129, Evalue=3e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYW_XANAC (Q8PFH5)

Other databases:

- EMBL:   AE008923
- RefSeq:   NP_644306.1
- ProteinModelPortal:   Q8PFH5
- SMR:   Q8PFH5
- GeneID:   1158077
- GenomeReviews:   AE008923_GR
- KEGG:   xac:XAC4006
- NMPDR:   fig|190486.1.peg.3950
- HOGENOM:   HBG293263
- OMA:   YFIADYH
- ProtClustDB:   PRK12284
- BioCyc:   XAXO190486:XAC4006-MONOMER
- BRENDA:   6.1.1.2
- GO:   GO:0005737
- HAMAP:   MF_00140_B
- InterPro:   IPR002305
- InterPro:   IPR010879
- InterPro:   IPR014729
- InterPro:   IPR002306
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10055
- PRINTS:   PR01039
- TIGRFAMs:   TIGR00233

Pfam domain/function: PF00579 tRNA-synt_1b

EC number: =6.1.1.2

Molecular weight: Translated: 47045; Mature: 46914

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 208-208

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSMTTRVLTGITTSGTPHLGNYVGAIRPAIQASAGADAESFYFLADLHSLIKAQDPART
CCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHH
QRSTLEIAATWLACGLDPDKVWFYRQSDVPETTELMWLLTCVAGKGILNRAHAYKAAVDR
HHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHC
NRADGEDEDAGVTAGLFMYPVLMAADILIFNAHQVPVGRDQIQHIEMARDFAQRFNHVYG
CCCCCCCCCCCCHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHC
REFFTLPEAVIDEQVSTLPGLDGRKMSKSYGNTIPLFAPREELRKLVFSILTDSRAPGQA
CHHHCCCHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCC
KDTQGSALFQLYQAFATPQESAAFAQAFADGIGWGDAKQQVFERIDQEIAPLRTRYEGLI
CCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
AEPARIEAILRAGGARLRARYATPLLAELRDAVGLRDLSSQAATAAVHASEKIALPVFKQ
CCHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHCHHHHHHHHHHHHHHCCCCHHHHHHHH
YRESDGQFYFKLNDGAGALLLQSDGFASPRDAGQVIARLKQAAQASDLQLPGVHAQVDAD
HHCCCCEEEEEEECCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHH
VVLAAMDALREA
HHHHHHHHHHCC
>Mature Secondary Structure 
TSMTTRVLTGITTSGTPHLGNYVGAIRPAIQASAGADAESFYFLADLHSLIKAQDPART
CCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHH
QRSTLEIAATWLACGLDPDKVWFYRQSDVPETTELMWLLTCVAGKGILNRAHAYKAAVDR
HHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHC
NRADGEDEDAGVTAGLFMYPVLMAADILIFNAHQVPVGRDQIQHIEMARDFAQRFNHVYG
CCCCCCCCCCCCHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHC
REFFTLPEAVIDEQVSTLPGLDGRKMSKSYGNTIPLFAPREELRKLVFSILTDSRAPGQA
CHHHCCCHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCC
KDTQGSALFQLYQAFATPQESAAFAQAFADGIGWGDAKQQVFERIDQEIAPLRTRYEGLI
CCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
AEPARIEAILRAGGARLRARYATPLLAELRDAVGLRDLSSQAATAAVHASEKIALPVFKQ
CCHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHCHHHHHHHHHHHHHHCCCCHHHHHHHH
YRESDGQFYFKLNDGAGALLLQSDGFASPRDAGQVIARLKQAAQASDLQLPGVHAQVDAD
HHCCCCEEEEEEECCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHH
VVLAAMDALREA
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12024217