Click here to switch to the map view.

The map label for this gene is speE

Identifier: 21244643

GI number: 21244643

Start: 4615481

End: 4616338

Strand: Direct

Name: speE

Synonym: XAC3924

Alternate gene names: 21244643

Gene position: 4615481-4616338 (Clockwise)

Preceding gene: 21244638

Following gene: 21244646

Centisome position: 89.18

GC content: 63.05

Gene sequence:

>858_bases
ATGAGCGCCAACGACAACTGGTACATCGAACACTTCCAGCCCACCGGCTCGGCGATCGGCTTCCGCATCAGCGGCAAGCT
GGACGAGGTGCAGTCCCCGTTCCAGAAGATCGAGATCTACCAGACCACCGATTGGGGCAAGCTGATGCTGATCGACGGCG
CGGTGATGCTCACCAGCCGCGACAATTTCTTCTACCATGAGATGATCAGTCACCCGGCACTGTTCACCCACCCCGCGCCC
AAGCGCGTGGTGATCATCGGCGGCGGCGACTGCGGCACGCTGCGCGAGGTGCTCAAGCACCCGGGCGTGGAAAGCGCCAC
CCAGTGCGATATCGATGAGCAGGTCACCCGCATGTCGGAGAAGTATTTTCCCGAGCTGTGCGATTCAAACCACGACGCGC
GCGCCGAACTGCTGTTCGACGATGGCGTGGCCTATATGGCCAATTGCCAGGCCGGCAGCGTGGACATCGTGATCGTCGAT
TCCACCGACCCGGTGGGCCCGGCCGAAGGCCTGTTCAACAAGGCCTTCTACGAGAGCTGCTTCAAGGCCTTGAAGGACGA
CGGCATCCTGGTGCAGCAGTCCGAATCGCCGCTGGCGCTGCTGGACCTGATCAATGAAATGCGCACCGAAATGGGCAAGG
CCGGCTTCCAGTCGTTCAAGACCCTGCCGTTCCCGCAGCCGTGCTACCCCACCGGCTGGTGGAGCGTGACCATGGCCAGC
AAGCAGGCCAAGGCCGATTTCGCGTTCCGCCAGGACGCTGCGCAGGCCAAGGGTTTCGACACCCTGTACTACACCGCCCA
TCTGCACACCGGCGTGCTGGTTGCCCCGCCGTTCGTGGCCAAGGCGCTGGGCGAGTAA

Upstream 100 bases:

>100_bases
AGTTTGGCTGCGCGAGGCCGTGCCCGACCCTCATCCGGCGCTTTGCGCCACCTTCTCCCGCTGGGAGAAGGAACAGCCCA
CTTCTTTTTTAGGACTTTGC

Downstream 100 bases:

>100_bases
TCGCGGCTGGCCTAGCATGGCCAGCAAGTGCAGACAGCAGGTGTTGAATCGTGGCGCTTGAATGGACGCGGCGGGTTCCA
GCGACCGGCCGCGCCCGCTT

Product: spermidine synthase

Products: NA

Alternate protein names: Putrescine aminopropyltransferase; PAPT; SPDSY

Number of amino acids: Translated: 285; Mature: 284

Protein sequence:

>285_residues
MSANDNWYIEHFQPTGSAIGFRISGKLDEVQSPFQKIEIYQTTDWGKLMLIDGAVMLTSRDNFFYHEMISHPALFTHPAP
KRVVIIGGGDCGTLREVLKHPGVESATQCDIDEQVTRMSEKYFPELCDSNHDARAELLFDDGVAYMANCQAGSVDIVIVD
STDPVGPAEGLFNKAFYESCFKALKDDGILVQQSESPLALLDLINEMRTEMGKAGFQSFKTLPFPQPCYPTGWWSVTMAS
KQAKADFAFRQDAAQAKGFDTLYYTAHLHTGVLVAPPFVAKALGE

Sequences:

>Translated_285_residues
MSANDNWYIEHFQPTGSAIGFRISGKLDEVQSPFQKIEIYQTTDWGKLMLIDGAVMLTSRDNFFYHEMISHPALFTHPAP
KRVVIIGGGDCGTLREVLKHPGVESATQCDIDEQVTRMSEKYFPELCDSNHDARAELLFDDGVAYMANCQAGSVDIVIVD
STDPVGPAEGLFNKAFYESCFKALKDDGILVQQSESPLALLDLINEMRTEMGKAGFQSFKTLPFPQPCYPTGWWSVTMAS
KQAKADFAFRQDAAQAKGFDTLYYTAHLHTGVLVAPPFVAKALGE
>Mature_284_residues
SANDNWYIEHFQPTGSAIGFRISGKLDEVQSPFQKIEIYQTTDWGKLMLIDGAVMLTSRDNFFYHEMISHPALFTHPAPK
RVVIIGGGDCGTLREVLKHPGVESATQCDIDEQVTRMSEKYFPELCDSNHDARAELLFDDGVAYMANCQAGSVDIVIVDS
TDPVGPAEGLFNKAFYESCFKALKDDGILVQQSESPLALLDLINEMRTEMGKAGFQSFKTLPFPQPCYPTGWWSVTMASK
QAKADFAFRQDAAQAKGFDTLYYTAHLHTGVLVAPPFVAKALGE

Specific function: Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM), which serves as an aminopropyl donor

COG id: COG0421

COG function: function code E; Spermidine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the spermidine/spermine synthase family

Homologues:

Organism=Homo sapiens, GI63253298, Length=276, Percent_Identity=36.5942028985507, Blast_Score=186, Evalue=2e-47,
Organism=Escherichia coli, GI1786312, Length=272, Percent_Identity=33.8235294117647, Blast_Score=150, Evalue=9e-38,
Organism=Caenorhabditis elegans, GI25154106, Length=272, Percent_Identity=37.5, Blast_Score=166, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI212645616, Length=322, Percent_Identity=31.9875776397516, Blast_Score=151, Evalue=3e-37,
Organism=Saccharomyces cerevisiae, GI6325326, Length=274, Percent_Identity=35.036496350365, Blast_Score=179, Evalue=4e-46,
Organism=Saccharomyces cerevisiae, GI6323175, Length=291, Percent_Identity=33.3333333333333, Blast_Score=157, Evalue=2e-39,
Organism=Drosophila melanogaster, GI24645443, Length=288, Percent_Identity=34.7222222222222, Blast_Score=174, Evalue=5e-44,
Organism=Drosophila melanogaster, GI21355941, Length=219, Percent_Identity=36.0730593607306, Blast_Score=135, Evalue=3e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SPEE_XANAC (Q8PFQ4)

Other databases:

- EMBL:   AE008923
- RefSeq:   NP_644225.1
- ProteinModelPortal:   Q8PFQ4
- SMR:   Q8PFQ4
- GeneID:   1157995
- GenomeReviews:   AE008923_GR
- KEGG:   xac:XAC3924
- NMPDR:   fig|190486.1.peg.3869
- HOGENOM:   HBG635113
- OMA:   VKHPLVE
- ProtClustDB:   PRK00811
- BioCyc:   XAXO190486:XAC3924-MONOMER
- BRENDA:   2.5.1.16
- HAMAP:   MF_00198
- InterPro:   IPR001045
- PANTHER:   PTHR11558
- TIGRFAMs:   TIGR00417

Pfam domain/function: PF01564 Spermine_synth

EC number: =2.5.1.16

Molecular weight: Translated: 31553; Mature: 31422

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS01330 SPERMIDINE_SYNTHASE_1; PS51006 SPERMIDINE_SYNTHASE_2

Important sites: BINDING 35-35 BINDING 90-90 BINDING 110-110 BINDING 160-160 BINDING 163-163

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSANDNWYIEHFQPTGSAIGFRISGKLDEVQSPFQKIEIYQTTDWGKLMLIDGAVMLTSR
CCCCCCEEEEEEECCCCEEEEEECCCHHHHHCCHHHEEEEEECCCCEEEEEECEEEEEEC
DNFFYHEMISHPALFTHPAPKRVVIIGGGDCGTLREVLKHPGVESATQCDIDEQVTRMSE
CCCHHHHHHCCCEEEECCCCCEEEEEECCCCHHHHHHHHCCCCCCCHHCCHHHHHHHHHH
KYFPELCDSNHDARAELLFDDGVAYMANCQAGSVDIVIVDSTDPVGPAEGLFNKAFYESC
HHHHHHHCCCCCCEEEEEEECCCEEEECCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHH
FKALKDDGILVQQSESPLALLDLINEMRTEMGKAGFQSFKTLPFPQPCYPTGWWSVTMAS
HHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECC
KQAKADFAFRQDAAQAKGFDTLYYTAHLHTGVLVAPPFVAKALGE
HHHHHHHHHHHHHHHHCCCEEEEEEEEECCCEEECCHHHHHHHCC
>Mature Secondary Structure 
SANDNWYIEHFQPTGSAIGFRISGKLDEVQSPFQKIEIYQTTDWGKLMLIDGAVMLTSR
CCCCCEEEEEEECCCCEEEEEECCCHHHHHCCHHHEEEEEECCCCEEEEEECEEEEEEC
DNFFYHEMISHPALFTHPAPKRVVIIGGGDCGTLREVLKHPGVESATQCDIDEQVTRMSE
CCCHHHHHHCCCEEEECCCCCEEEEEECCCCHHHHHHHHCCCCCCCHHCCHHHHHHHHHH
KYFPELCDSNHDARAELLFDDGVAYMANCQAGSVDIVIVDSTDPVGPAEGLFNKAFYESC
HHHHHHHCCCCCCEEEEEEECCCEEEECCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHH
FKALKDDGILVQQSESPLALLDLINEMRTEMGKAGFQSFKTLPFPQPCYPTGWWSVTMAS
HHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECC
KQAKADFAFRQDAAQAKGFDTLYYTAHLHTGVLVAPPFVAKALGE
HHHHHHHHHHHHHHHHCCCEEEEEEEEECCCEEECCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217