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The map label for this gene is tyrS

Identifier: 21244616

GI number: 21244616

Start: 4582318

End: 4583529

Strand: Reverse

Name: tyrS

Synonym: XAC3897

Alternate gene names: 21244616

Gene position: 4583529-4582318 (Counterclockwise)

Preceding gene: 21244619

Following gene: 21244613

Centisome position: 88.56

GC content: 63.37

Gene sequence:

>1212_bases
TTGACCACTATCGAAGAATCCCTCGCACTCATTGGCCGCGGCACCGAAGAGATCCTCAAGCTCGATCAACTGCAGGCGCG
GCTCGCCACCGGTAAGCCGCTGCGGGTGAAGGCCGGCTTCGATCCCACCGCGCCGGATCTGCATCTGGGCCATACCGTGC
TGCTCAACAAGATGCGGCAATTCCAGCAGCTCGGCCATCAGGTGATCTTCCTGATTGGCGATTTCACCGGCATGATCGGA
GACCCGAGCGGCAAGAACGCCACCCGCAAGCCGCTCAGCCGCGAGGATGTGCTGGCCAACGCACGGACCTACGAAGAGCA
GGTCTTCAAGATCCTGGACCGCGAACGCACCGAAGTGCGCTTCAACTCCGAATGGTTCGGCCAGATGAGCGCGGCCGACA
TGATCAAGCTCTCTGCCCAGCACACCGTCGCGCGCATGCTCGAACGCGACGATTTCGCCAAGCGCTTCGGCAGCCAGCAG
CCGATCGCCATCCACGAATTCCTCTATCCATTAGTGCAGGGCTACGATTCGGTTGCCCTGAAGGCGGACGTCGAGCTCGG
CGGCACCGATCAGAAGTTCAACCTGTTGATGGGGCGTGGCTTGCAGGAGCATTACGGCCAGGCGCCGCAGATCGTGCTGA
CCATGCCATTGCTGGAAGGCCTGGACGGCGTGGCCAAGATGTCCAAGTCACTGGGCAATTACATCGGCATCAACGAGCCG
GCCATCGACATCGTCACCAAGACGATGAAGATCGGCGACGCGCTGACCTGGCGCTGGATCGACCTGCTGTCCTTCGATAT
CAGCATGGCCGAAGCGGCACGCCTGAAGGAGGAGGTCGCCTCCGGGGAGTTGCACCCGCGCGAGGTCAAGCTGCGCCTGG
CACGCGAACTGGCCACCCGGTTCCACGATGCCGCCACCGCAGAGCAGGCCATTGCCGGCTGGCATGCGGTGGTGACAGGG
CAGGGCGACACCGGTCTGCTGCCGCTGCAGGAAGTGGTGGTTCCCGCCGAAGGCCTGCGCCTCGCCGGTCTCCTGACTGC
CGCCGGGCTGACGCCCAGCAATTCCGAAGCCACCCGCAAACTCAAGGAGCGCGCGGTCAAGATCGACGGCGAAGTGGTGG
AGGATCCCGGTCGCGTCTTCACGCAGGGCTTTGAGGGGGTCATCCAGGTGGGCAAGCGCAATTTTGCCCGCGTATCGCTC
GTCACTGGCTAA

Upstream 100 bases:

>100_bases
TCAAACCATTGAGCCTGTTCATTATTTTCACTTGGCGCGGAATTAACCCGCGTTTAACATCATTCACGTTTTTGACGGCA
AGCGCCGCTGGAGTCTGTGG

Downstream 100 bases:

>100_bases
AGCGCACCGAGGTTGCTGCAGATCGGCAATCTCGGTGGCCCCACATGTCTTACCTGTTAGATGGAAGTAGAAATCCAAGG
CCCTCAGAGGTTTCAAGCCA

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS

Number of amino acids: Translated: 403; Mature: 402

Protein sequence:

>403_residues
MTTIEESLALIGRGTEEILKLDQLQARLATGKPLRVKAGFDPTAPDLHLGHTVLLNKMRQFQQLGHQVIFLIGDFTGMIG
DPSGKNATRKPLSREDVLANARTYEEQVFKILDRERTEVRFNSEWFGQMSAADMIKLSAQHTVARMLERDDFAKRFGSQQ
PIAIHEFLYPLVQGYDSVALKADVELGGTDQKFNLLMGRGLQEHYGQAPQIVLTMPLLEGLDGVAKMSKSLGNYIGINEP
AIDIVTKTMKIGDALTWRWIDLLSFDISMAEAARLKEEVASGELHPREVKLRLARELATRFHDAATAEQAIAGWHAVVTG
QGDTGLLPLQEVVVPAEGLRLAGLLTAAGLTPSNSEATRKLKERAVKIDGEVVEDPGRVFTQGFEGVIQVGKRNFARVSL
VTG

Sequences:

>Translated_403_residues
MTTIEESLALIGRGTEEILKLDQLQARLATGKPLRVKAGFDPTAPDLHLGHTVLLNKMRQFQQLGHQVIFLIGDFTGMIG
DPSGKNATRKPLSREDVLANARTYEEQVFKILDRERTEVRFNSEWFGQMSAADMIKLSAQHTVARMLERDDFAKRFGSQQ
PIAIHEFLYPLVQGYDSVALKADVELGGTDQKFNLLMGRGLQEHYGQAPQIVLTMPLLEGLDGVAKMSKSLGNYIGINEP
AIDIVTKTMKIGDALTWRWIDLLSFDISMAEAARLKEEVASGELHPREVKLRLARELATRFHDAATAEQAIAGWHAVVTG
QGDTGLLPLQEVVVPAEGLRLAGLLTAAGLTPSNSEATRKLKERAVKIDGEVVEDPGRVFTQGFEGVIQVGKRNFARVSL
VTG
>Mature_402_residues
TTIEESLALIGRGTEEILKLDQLQARLATGKPLRVKAGFDPTAPDLHLGHTVLLNKMRQFQQLGHQVIFLIGDFTGMIGD
PSGKNATRKPLSREDVLANARTYEEQVFKILDRERTEVRFNSEWFGQMSAADMIKLSAQHTVARMLERDDFAKRFGSQQP
IAIHEFLYPLVQGYDSVALKADVELGGTDQKFNLLMGRGLQEHYGQAPQIVLTMPLLEGLDGVAKMSKSLGNYIGINEPA
IDIVTKTMKIGDALTWRWIDLLSFDISMAEAARLKEEVASGELHPREVKLRLARELATRFHDAATAEQAIAGWHAVVTGQ
GDTGLLPLQEVVVPAEGLRLAGLLTAAGLTPSNSEATRKLKERAVKIDGEVVEDPGRVFTQGFEGVIQVGKRNFARVSLV
TG

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr)

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain

Homologues:

Organism=Homo sapiens, GI94681057, Length=425, Percent_Identity=26.3529411764706, Blast_Score=100, Evalue=4e-21,
Organism=Escherichia coli, GI1787925, Length=416, Percent_Identity=29.0865384615385, Blast_Score=139, Evalue=4e-34,
Organism=Caenorhabditis elegans, GI25145802, Length=210, Percent_Identity=32.8571428571429, Blast_Score=102, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6325160, Length=388, Percent_Identity=25, Blast_Score=93, Evalue=8e-20,
Organism=Drosophila melanogaster, GI20130383, Length=403, Percent_Identity=25.3101736972705, Blast_Score=119, Evalue=3e-27,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYY_XANAC (Q8PFT1)

Other databases:

- EMBL:   AE008923
- RefSeq:   NP_644198.1
- ProteinModelPortal:   Q8PFT1
- SMR:   Q8PFT1
- GeneID:   1157968
- GenomeReviews:   AE008923_GR
- NMPDR:   fig|190486.1.peg.3842
- HOGENOM:   HBG288125
- OMA:   YVVQVGK
- ProtClustDB:   PRK05912
- BioCyc:   XAXO190486:XAC3897-MONOMER
- BRENDA:   6.1.1.1
- GO:   GO:0005737
- HAMAP:   MF_02007
- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11766
- PRINTS:   PR01040
- SMART:   SM00363
- TIGRFAMs:   TIGR00234

Pfam domain/function: PF00579 tRNA-synt_1b

EC number: =6.1.1.1

Molecular weight: Translated: 44332; Mature: 44200

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: PS00178 AA_TRNA_LIGASE_I; PS50889 S4

Important sites: BINDING 229-229

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTIEESLALIGRGTEEILKLDQLQARLATGKPLRVKAGFDPTAPDLHLGHTVLLNKMRQ
CCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHHHHHHHHH
FQQLGHQVIFLIGDFTGMIGDPSGKNATRKPLSREDVLANARTYEEQVFKILDRERTEVR
HHHHCHHEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHEE
FNSEWFGQMSAADMIKLSAQHTVARMLERDDFAKRFGSQQPIAIHEFLYPLVQGYDSVAL
ECHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCEEE
KADVELGGTDQKFNLLMGRGLQEHYGQAPQIVLTMPLLEGLDGVAKMSKSLGNYIGINEP
EEEEEECCCCCHHHHHHCCCCHHHCCCCCEEEEECHHHHCCHHHHHHHHHHCCCCCCCCC
AIDIVTKTMKIGDALTWRWIDLLSFDISMAEAARLKEEVASGELHPREVKLRLARELATR
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
FHDAATAEQAIAGWHAVVTGQGDTGLLPLQEVVVPAEGLRLAGLLTAAGLTPSNSEATRK
HHHHHHHHHHHHCCEEEEECCCCCCCCCHHHHHCCCCCCHHHHHHHHCCCCCCCHHHHHH
LKERAVKIDGEVVEDPGRVFTQGFEGVIQVGKRNFARVSLVTG
HHHHHHHCCCEEECCCHHHHHHHHHHHHHHCCCCCEEEEEECC
>Mature Secondary Structure 
TTIEESLALIGRGTEEILKLDQLQARLATGKPLRVKAGFDPTAPDLHLGHTVLLNKMRQ
CCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHHHHHHHHH
FQQLGHQVIFLIGDFTGMIGDPSGKNATRKPLSREDVLANARTYEEQVFKILDRERTEVR
HHHHCHHEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHEE
FNSEWFGQMSAADMIKLSAQHTVARMLERDDFAKRFGSQQPIAIHEFLYPLVQGYDSVAL
ECHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCEEE
KADVELGGTDQKFNLLMGRGLQEHYGQAPQIVLTMPLLEGLDGVAKMSKSLGNYIGINEP
EEEEEECCCCCHHHHHHCCCCHHHCCCCCEEEEECHHHHCCHHHHHHHHHHCCCCCCCCC
AIDIVTKTMKIGDALTWRWIDLLSFDISMAEAARLKEEVASGELHPREVKLRLARELATR
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
FHDAATAEQAIAGWHAVVTGQGDTGLLPLQEVVVPAEGLRLAGLLTAAGLTPSNSEATRK
HHHHHHHHHHHHCCEEEEECCCCCCCCCHHHHHCCCCCCHHHHHHHHCCCCCCCHHHHHH
LKERAVKIDGEVVEDPGRVFTQGFEGVIQVGKRNFARVSLVTG
HHHHHHHCCCEEECCCHHHHHHHHHHHHHHCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12024217