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The map label for this gene is dnaG [H]

Identifier: 21244600

GI number: 21244600

Start: 4558729

End: 4560477

Strand: Direct

Name: dnaG [H]

Synonym: XAC3876

Alternate gene names: 21244600

Gene position: 4558729-4560477 (Clockwise)

Preceding gene: 21244599

Following gene: 21244601

Centisome position: 88.08

GC content: 65.69

Gene sequence:

>1749_bases
ATGGCCCGCATCCCCGACGCGTTTATCGACGAACTGCTTGCACGCACCGACATTGTCGAGGTGGTGGGCGGTCGCGTGCC
GCTCAAGCGTCAGGGCAAGGAATATTCGGCGCGCTGCCCGTTCCACGACGAGCGTTCGGCCTCGTTCACGGTGTCGCCGA
CCAAGCAGTTCTATCACTGCTTCGGCTGTGGCGCGCACGGCACCGCAATCAGTTTCCTGATGAATTACGACCGCCTCGAA
TTTCTCGATGCGGTCGACGAGCTGGCCAAGCGCGCCGGCATGGACATCCCGCGCGAGACGCAGCAACGCACGCCGCAACA
GCAGGACGACAGCCGCGAGCTGTACTCGGCACTGGAAGCGGCCACCAAGTTCTTCCAGCGCCAGCTCGAGGGCAGCGGAC
GCGCTCGCGACTATCTGGACGGGCGCGGCGTGGATGCCGACAACCGCGCGCGTTTCCAGATCGGCTATGCGCCCGACGGC
TACAGCGCATTGAAGGACACCCTGGGCACCGATGCGCGGCGCATGAGCGTGCTCGAGCGCGCCGGGCTGTTTTCCAAGAA
CGATCGCGGCCATGTGTACGACAAGTTCCGCGACCGGGTGATGTTCCCGATCTTCGACCGCCGCGGCCGCGTGATCGCCT
TCGGCGGACGCATCATGGGCGCGCCGGCCGATGGCCGCGACCCGGGGCCGAAGTACCTCAACTCGCCGGAAACCGCGCTG
TTCCACAAGGGCCGCGAGTTGTACGGCCTGTGGCAGGTGCGGCAGGCCAATCAGAAGATCGAGCGGCTGATCGTGGTGGA
AGGCTACATGGACGTGGTCTCGCTGTTCCAGTTCGGCGTCACCCAGGCGGTGGCCACGCTGGGCACCGCCACCACGCCCG
AACATGCCGAGCTGCTGTTCCGCAACGCGCCGGATGTGTATTTCTGTTTCGATGGCGACAACGCCGGGCGCAAGGCCGGC
TGGCGCGCGCTCGAATCGGTGCTGCCGCGCATGAAGGATGGCCGCCAGGCGTTTTTCCTGTTCCTGCCCGATGGCGAGGA
CCCGGACACCATCGTACGCAAGGAAGGCGCACAGGCCTTCGATCAGCGCCTGAAACAGGCCACGCCGCTGTCGCAGTTCT
TCTTCGACGAGATGTCGCGTGAGATCAACCTGCACACGCTGGACGGCAAGGCGCGCCTGGCCGAACGTGCGCGCCCGCTG
TTGGCGCAAATCCCCGAGGGCGCATTCGGCGATCTGATGAAACAGGAACTGGCGCGCCTGACCGGGGTTGGCGCCAATGC
GTCTGCGCAGCAGCCCGCACCCAGGCCGCGGCCGCCTGCCCGCATGGGCGCGCCCACACAGAAACGCAGCCTGGTGCGTG
CGTCCATCGCCATCCTGCTGCAACGGCCGTCATTGGCGATGAGCCTGGAGGGCGTGCATGATTTTTCCGGGCTGCGCCTG
CCCGGGATCGACTTGTTGATGGAATTGCTGGACCTGGTCCGGCAACGCCCGGAAATCAGCACGGGCGCGCTGCTGGAGCA
CTTCGCCGAGCGCGAGGAACTCGCTGCCTTGCAGAAGCTGGCGGCGCAGGAGCTTCCCGGCGACGAACACAGTTGGACGC
TTGAGCTGCATGACGTCGTCGCGCAACTCGACAAGCAGTTGCTGCGTCAGCGCGTGGAAGAGCTGCAGGCCAAACAGCGC
GCGCAAGGGCTGGACGACACCGACAAATACGAAATGCGCGAACTGCTCAAGGCGCTCGCAGCGTTGTGA

Upstream 100 bases:

>100_bases
CGCTGCCACGCGGCTGAGGACTCTGCTGCAGTGCCGGCCTCCCCGGCCTCGTCGGCGGGGGCGACGAAGTACTGGCAGGC
CGCTGGGATATCCTAGCGGG

Downstream 100 bases:

>100_bases
TGCAGCGCTGTGCGGCTGGATGCAGCGCAAGGCAATCGCGCACCGCCGGGCCGTTTCAGGCGTGAACGGTGGCGTCGATG
CCACAGCACGCATCGTAGAA

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 582; Mature: 581

Protein sequence:

>582_residues
MARIPDAFIDELLARTDIVEVVGGRVPLKRQGKEYSARCPFHDERSASFTVSPTKQFYHCFGCGAHGTAISFLMNYDRLE
FLDAVDELAKRAGMDIPRETQQRTPQQQDDSRELYSALEAATKFFQRQLEGSGRARDYLDGRGVDADNRARFQIGYAPDG
YSALKDTLGTDARRMSVLERAGLFSKNDRGHVYDKFRDRVMFPIFDRRGRVIAFGGRIMGAPADGRDPGPKYLNSPETAL
FHKGRELYGLWQVRQANQKIERLIVVEGYMDVVSLFQFGVTQAVATLGTATTPEHAELLFRNAPDVYFCFDGDNAGRKAG
WRALESVLPRMKDGRQAFFLFLPDGEDPDTIVRKEGAQAFDQRLKQATPLSQFFFDEMSREINLHTLDGKARLAERARPL
LAQIPEGAFGDLMKQELARLTGVGANASAQQPAPRPRPPARMGAPTQKRSLVRASIAILLQRPSLAMSLEGVHDFSGLRL
PGIDLLMELLDLVRQRPEISTGALLEHFAEREELAALQKLAAQELPGDEHSWTLELHDVVAQLDKQLLRQRVEELQAKQR
AQGLDDTDKYEMRELLKALAAL

Sequences:

>Translated_582_residues
MARIPDAFIDELLARTDIVEVVGGRVPLKRQGKEYSARCPFHDERSASFTVSPTKQFYHCFGCGAHGTAISFLMNYDRLE
FLDAVDELAKRAGMDIPRETQQRTPQQQDDSRELYSALEAATKFFQRQLEGSGRARDYLDGRGVDADNRARFQIGYAPDG
YSALKDTLGTDARRMSVLERAGLFSKNDRGHVYDKFRDRVMFPIFDRRGRVIAFGGRIMGAPADGRDPGPKYLNSPETAL
FHKGRELYGLWQVRQANQKIERLIVVEGYMDVVSLFQFGVTQAVATLGTATTPEHAELLFRNAPDVYFCFDGDNAGRKAG
WRALESVLPRMKDGRQAFFLFLPDGEDPDTIVRKEGAQAFDQRLKQATPLSQFFFDEMSREINLHTLDGKARLAERARPL
LAQIPEGAFGDLMKQELARLTGVGANASAQQPAPRPRPPARMGAPTQKRSLVRASIAILLQRPSLAMSLEGVHDFSGLRL
PGIDLLMELLDLVRQRPEISTGALLEHFAEREELAALQKLAAQELPGDEHSWTLELHDVVAQLDKQLLRQRVEELQAKQR
AQGLDDTDKYEMRELLKALAAL
>Mature_581_residues
ARIPDAFIDELLARTDIVEVVGGRVPLKRQGKEYSARCPFHDERSASFTVSPTKQFYHCFGCGAHGTAISFLMNYDRLEF
LDAVDELAKRAGMDIPRETQQRTPQQQDDSRELYSALEAATKFFQRQLEGSGRARDYLDGRGVDADNRARFQIGYAPDGY
SALKDTLGTDARRMSVLERAGLFSKNDRGHVYDKFRDRVMFPIFDRRGRVIAFGGRIMGAPADGRDPGPKYLNSPETALF
HKGRELYGLWQVRQANQKIERLIVVEGYMDVVSLFQFGVTQAVATLGTATTPEHAELLFRNAPDVYFCFDGDNAGRKAGW
RALESVLPRMKDGRQAFFLFLPDGEDPDTIVRKEGAQAFDQRLKQATPLSQFFFDEMSREINLHTLDGKARLAERARPLL
AQIPEGAFGDLMKQELARLTGVGANASAQQPAPRPRPPARMGAPTQKRSLVRASIAILLQRPSLAMSLEGVHDFSGLRLP
GIDLLMELLDLVRQRPEISTGALLEHFAEREELAALQKLAAQELPGDEHSWTLELHDVVAQLDKQLLRQRVEELQAKQRA
QGLDDTDKYEMRELLKALAAL

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=588, Percent_Identity=45.0680272108844, Blast_Score=516, Evalue=1e-147,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016136
- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR013173
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF08278 DnaG_DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 65320; Mature: 65189

Theoretical pI: Translated: 6.92; Mature: 6.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARIPDAFIDELLARTDIVEVVGGRVPLKRQGKEYSARCPFHDERSASFTVSPTKQFYHC
CCCCCHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCCCCCCCEEECCHHHHHHH
FGCGAHGTAISFLMNYDRLEFLDAVDELAKRAGMDIPRETQQRTPQQQDDSRELYSALEA
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCHHHHHHHHHHH
ATKFFQRQLEGSGRARDYLDGRGVDADNRARFQIGYAPDGYSALKDTLGTDARRMSVLER
HHHHHHHHHCCCCCCHHCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHCCHHHHHHHHHH
AGLFSKNDRGHVYDKFRDRVMFPIFDRRGRVIAFGGRIMGAPADGRDPGPKYLNSPETAL
HCCCCCCCCCCHHHHHHCCEECCEECCCCCEEEECCEEECCCCCCCCCCCCCCCCCCHHH
FHKGRELYGLWQVRQANQKIERLIVVEGYMDVVSLFQFGVTQAVATLGTATTPEHAELLF
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
RNAPDVYFCFDGDNAGRKAGWRALESVLPRMKDGRQAFFLFLPDGEDPDTIVRKEGAQAF
CCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHH
DQRLKQATPLSQFFFDEMSREINLHTLDGKARLAERARPLLAQIPEGAFGDLMKQELARL
HHHHHHCCCHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
TGVGANASAQQPAPRPRPPARMGAPTQKRSLVRASIAILLQRPSLAMSLEGVHDFSGLRL
HCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHEECCCCCCCCCCCC
PGIDLLMELLDLVRQRPEISTGALLEHFAEREELAALQKLAAQELPGDEHSWTLELHDVV
CCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHH
AQLDKQLLRQRVEELQAKQRAQGLDDTDKYEMRELLKALAAL
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ARIPDAFIDELLARTDIVEVVGGRVPLKRQGKEYSARCPFHDERSASFTVSPTKQFYHC
CCCCHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCCCCCCCEEECCHHHHHHH
FGCGAHGTAISFLMNYDRLEFLDAVDELAKRAGMDIPRETQQRTPQQQDDSRELYSALEA
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCHHHHHHHHHHH
ATKFFQRQLEGSGRARDYLDGRGVDADNRARFQIGYAPDGYSALKDTLGTDARRMSVLER
HHHHHHHHHCCCCCCHHCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHCCHHHHHHHHHH
AGLFSKNDRGHVYDKFRDRVMFPIFDRRGRVIAFGGRIMGAPADGRDPGPKYLNSPETAL
HCCCCCCCCCCHHHHHHCCEECCEECCCCCEEEECCEEECCCCCCCCCCCCCCCCCCHHH
FHKGRELYGLWQVRQANQKIERLIVVEGYMDVVSLFQFGVTQAVATLGTATTPEHAELLF
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
RNAPDVYFCFDGDNAGRKAGWRALESVLPRMKDGRQAFFLFLPDGEDPDTIVRKEGAQAF
CCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHH
DQRLKQATPLSQFFFDEMSREINLHTLDGKARLAERARPLLAQIPEGAFGDLMKQELARL
HHHHHHCCCHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
TGVGANASAQQPAPRPRPPARMGAPTQKRSLVRASIAILLQRPSLAMSLEGVHDFSGLRL
HCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHEECCCCCCCCCCCC
PGIDLLMELLDLVRQRPEISTGALLEHFAEREELAALQKLAAQELPGDEHSWTLELHDVV
CCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHH
AQLDKQLLRQRVEELQAKQRAQGLDDTDKYEMRELLKALAAL
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]