Click here to switch to the map view.

The map label for this gene is def

Identifier: 21244525

GI number: 21244525

Start: 4472009

End: 4472521

Strand: Reverse

Name: def

Synonym: XAC3801

Alternate gene names: 21244525

Gene position: 4472521-4472009 (Counterclockwise)

Preceding gene: 21244535

Following gene: 21244524

Centisome position: 86.42

GC content: 61.6

Gene sequence:

>513_bases
ATGGCTTTGCTCCCAATTCTTGAGTTTCCCGATCCGCGGCTGCGCACCAAGGCCGTGCCGGTCGATGCTGCCGAGGTCAC
CAGCCAGGCGTTCCAGACGCTGCTGGACGATATGTTCCACACCATGTACGAGGCGCCCGGCATTGGCCTGGCTGCCAGTC
AGGTGGACGTGCACAAGCGATTCATGGTGATCGACATCAGCGACGAGAAAAACCTGCCGCAGGTGTTCGTCAACCCGGAG
ATCGTGAGCAAACAGGGCGAGCAGCTGTATCAGGAAGGCTGCCTGTCGGTGCCCGGCATCTATGCCGATGTCAGCCGCGC
CGATGCGATCACCGTGCGCTATCTCGACCGCCAGGGCCAGCCGCAGGAACTGCATGCCGACGGGCTGCTGGCCGTGTGCA
TCCAGCACGAGATGGACCACCTGGACGGCAAGCTGTTCGTGGATTATCTCTCGCCGCTCAAGCGCGAAATGGTGCGCAAG
AAGCTGGCCAAACAGCGCAAGCATGTGGCTTGA

Upstream 100 bases:

>100_bases
CTGCGCACTATAGTCTAGAAACCGGGGCGCCACGCAAGCATTGCGCTAGAATGACACCGTCTTGCCGGCGCCGCGCCGGT
GTTTCCAGACGGGTAACCCC

Downstream 100 bases:

>100_bases
GGGCCGGGATTGGGGATTGGGGATTCGGGATTGGCAGATGCGGGTTTTTGCTGGCCTTTGAGTCCGCTGGTTTCAAATCG
TTGAGCGCTCATCTCGGTGC

Product: peptide deformylase

Products: NA

Alternate protein names: PDF 2; Polypeptide deformylase 2

Number of amino acids: Translated: 170; Mature: 169

Protein sequence:

>170_residues
MALLPILEFPDPRLRTKAVPVDAAEVTSQAFQTLLDDMFHTMYEAPGIGLAASQVDVHKRFMVIDISDEKNLPQVFVNPE
IVSKQGEQLYQEGCLSVPGIYADVSRADAITVRYLDRQGQPQELHADGLLAVCIQHEMDHLDGKLFVDYLSPLKREMVRK
KLAKQRKHVA

Sequences:

>Translated_170_residues
MALLPILEFPDPRLRTKAVPVDAAEVTSQAFQTLLDDMFHTMYEAPGIGLAASQVDVHKRFMVIDISDEKNLPQVFVNPE
IVSKQGEQLYQEGCLSVPGIYADVSRADAITVRYLDRQGQPQELHADGLLAVCIQHEMDHLDGKLFVDYLSPLKREMVRK
KLAKQRKHVA
>Mature_169_residues
ALLPILEFPDPRLRTKAVPVDAAEVTSQAFQTLLDDMFHTMYEAPGIGLAASQVDVHKRFMVIDISDEKNLPQVFVNPEI
VSKQGEQLYQEGCLSVPGIYADVSRADAITVRYLDRQGQPQELHADGLLAVCIQHEMDHLDGKLFVDYLSPLKREMVRKK
LAKQRKHVA

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family

Homologues:

Organism=Homo sapiens, GI11641243, Length=162, Percent_Identity=31.4814814814815, Blast_Score=77, Evalue=7e-15,
Organism=Escherichia coli, GI1789682, Length=164, Percent_Identity=54.8780487804878, Blast_Score=184, Evalue=2e-48,
Organism=Drosophila melanogaster, GI24645728, Length=158, Percent_Identity=32.2784810126582, Blast_Score=84, Evalue=7e-17,
Organism=Drosophila melanogaster, GI24645726, Length=157, Percent_Identity=30.5732484076433, Blast_Score=78, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DEF2_XANAC (Q8PG20)

Other databases:

- EMBL:   AE008923
- RefSeq:   NP_644107.1
- ProteinModelPortal:   Q8PG20
- SMR:   Q8PG20
- GeneID:   1157872
- GenomeReviews:   AE008923_GR
- KEGG:   xac:XAC3801
- NMPDR:   fig|190486.1.peg.3751
- HOGENOM:   HBG665227
- OMA:   RQLVDDM
- ProtClustDB:   PRK00150
- BioCyc:   XAXO190486:XAC3801-MONOMER
- BRENDA:   3.5.1.88
- GO:   GO:0006412
- HAMAP:   MF_00163
- InterPro:   IPR000181
- Gene3D:   G3DSA:3.90.45.10
- PANTHER:   PTHR10458
- PIRSF:   PIRSF004749
- PRINTS:   PR01576
- TIGRFAMs:   TIGR00079

Pfam domain/function: PF01327 Pep_deformylase; SSF56420 Fmet_deformylase

EC number: =3.5.1.88

Molecular weight: Translated: 19139; Mature: 19008

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: NA

Important sites: ACT_SITE 137-137

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALLPILEFPDPRLRTKAVPVDAAEVTSQAFQTLLDDMFHTMYEAPGIGLAASQVDVHKR
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHE
FMVIDISDEKNLPQVFVNPEIVSKQGEQLYQEGCLSVPGIYADVSRADAITVRYLDRQGQ
EEEEEECCCCCCCEEEECHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCEEEEEEECCCCC
PQELHADGLLAVCIQHEMDHLDGKLFVDYLSPLKREMVRKKLAKQRKHVA
CCHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ALLPILEFPDPRLRTKAVPVDAAEVTSQAFQTLLDDMFHTMYEAPGIGLAASQVDVHKR
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHE
FMVIDISDEKNLPQVFVNPEIVSKQGEQLYQEGCLSVPGIYADVSRADAITVRYLDRQGQ
EEEEEECCCCCCCEEEECHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCEEEEEEECCCCC
PQELHADGLLAVCIQHEMDHLDGKLFVDYLSPLKREMVRKKLAKQRKHVA
CCHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217