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The map label for this gene is phdB [H]

Identifier: 21244386

GI number: 21244386

Start: 4338761

End: 4340539

Strand: Reverse

Name: phdB [H]

Synonym: XAC3661

Alternate gene names: 21244386

Gene position: 4340539-4338761 (Counterclockwise)

Preceding gene: 77748735

Following gene: 21244385

Centisome position: 83.87

GC content: 67.34

Gene sequence:

>1779_bases
ATGGCCGAAATCAAGGAAGCACTTGTCCCCGATATCGGTGACTACAGCGACGTCCCGGTAATCGAGGTGCTGGTGTCCGT
CGGCGATACGGTCAGCAAGGACCAGAGCCTGGTCACGCTGGAATCGGACAAGGCCACCATGGAAGTGCCTTCGTCGGTGG
CTGGCGTGGTCAAGGAGATCAAGGTCAAGGTCGGCGATTCGCTCTCGCAGGGCGCGCTGGTGGCATTGATCGAAGTGGCC
GACGCCGGCGCCGATGCCGCCAAGCCCGCCGCTGCCGCTGCGCCCGCCGCACCGGCCAAGGCTGCGCCCGCCGCCACACC
GGCACCGGCTGCCAGGGCCGAATCTGCTGCGCCTGCGGCCTCTTCGGACGGCGGCGGCCTGATCGAAGCGCGTGTGCCCG
ATATCGGCGATTACACCGACATCCCGGTGATCGAAGTGCTGGTGGCCGTGGGCGATACCGTCGCCAAGGACCAGAGCCTG
GTCACGCTGGAATCGGACAAGGCCACGATGGAAGTCCCGTCCTCGGCCGCCGGCGTGGTCAAGGAACTCAAGGTCAAGGT
CGGCGACACGCTCTCGCAGGGCAATGTGGTGGCGATCATTGCAGCCGCCGACGGCGGCGCCGGTGCGGCGCAGTCGCCGG
CCAAGCCCACCACCGACACCGCCGAAACCGCGGGCAAGGTCGAGCCGGTTGCCGTCCCGGCCGAGCCGGACAAGCTGGCC
CAGCGCGAAATCGCGCAGGTGCAGGGCGCGCGTTCCGGCACCGGCACGCCGGCAGCGCAGGGCGGCCAGCCGTCCGCAGG
CAACCCGAGCAGCCCGCCGGTGACCTTCGATGCCGACAGCGTGCTGCCGTCCAAGGTGCCGTACGCCAGCCCGGTGGTGC
GCGTGTTCGCACGCGAACTGGGCGTGGACCTGAATCAGCTCAAGGGCAGCGAAAAGGGCGGCCGCATCACCCGTGAAGAC
GTGCAGCGCTTCGTCAAGGCCGCACTCAGCGGCGGCGCACCTGCCGCAGCCGGTGCCGCGCCGGCCGGTGGCGGCAATGG
CCTGAACCTGCTGGCGTGGCCGAAGGTGGACTTCAGCAAGTTCGGCGAGACCGAGACCCAGCCGCTGTCGCGCATCAAGA
AGGTTTCCGGTGCCAACCTGGCGCGCAACTGGGCAATGATTCCGCACGTCACCCAGTTCGAGTCGGCCGACATCACCGAC
CTGGAAGCCCTGCGCGTGGCGCTCAACAAAGAGAACGAGAAGGCCGGCATCAAGCTCACCATGCTCGCCTTCCTGGTCAA
GGCCAGCGCCGCGGCGCTGAAGAAGTTCCCCGAGTTCAACGCCTCGCTGGATGCGGCCGGCGACAACCTTACCCTGAAGA
AGTACTTCCATATCGGGTTTGCGGCCGATACGCCGAATGGCCTGGTCGTGCCGGTGATCCGCGATGTCGACAAGAAGGGC
GTGTTGCAGATCGCGCAGGAAAGCGGCGAGCTGGCCAAGAAGGCGCGCGACGGCAAGCTAGGCCCGGCCGACATGAGCGG
CGGCTGCTTCTCGATCAGCTCGCTCGGCGGCATCGGCGGCACGGCGTTCACGCCGATCATCAATGCGCCGGAAGTGGCGA
TCCTGGGCGTGTCCAAGTCGGCGATGCAGCCGGTCTGGAACGGCAAGGATTTCGCGCCGAAGCTGATGCTGCCGTTGTCG
CTGAGCTACGACCACCGGGTCATCGACGGCGCCCTGGCAGCCCGTTTCACCACCTATCTCAGCCAGGTGCTGGCCGACAT
GCGTCGCGTCCTGCTGTGA

Upstream 100 bases:

>100_bases
TCCTGCCTGGCGCGCGACTTTTTGCACGGCAATGGTTGGCGCTGCGGCCCGCAGCCGGCCACAATAGGGGTTTCCCCATT
CTGTTGCCGGAGTTCCCCGT

Downstream 100 bases:

>100_bases
GGGCCGCGCTGCTGACCATCGCCGCGCTCGGCGTGCTGCCGTGGACAACCGCGGCCGCACGCGAATGCGAGAGCACGCTC
GGCCGCGGCTGGCCGCCGGC

Product: dihydrolipoamide acetyltransferase

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 592; Mature: 591

Protein sequence:

>592_residues
MAEIKEALVPDIGDYSDVPVIEVLVSVGDTVSKDQSLVTLESDKATMEVPSSVAGVVKEIKVKVGDSLSQGALVALIEVA
DAGADAAKPAAAAAPAAPAKAAPAATPAPAARAESAAPAASSDGGGLIEARVPDIGDYTDIPVIEVLVAVGDTVAKDQSL
VTLESDKATMEVPSSAAGVVKELKVKVGDTLSQGNVVAIIAAADGGAGAAQSPAKPTTDTAETAGKVEPVAVPAEPDKLA
QREIAQVQGARSGTGTPAAQGGQPSAGNPSSPPVTFDADSVLPSKVPYASPVVRVFARELGVDLNQLKGSEKGGRITRED
VQRFVKAALSGGAPAAAGAAPAGGGNGLNLLAWPKVDFSKFGETETQPLSRIKKVSGANLARNWAMIPHVTQFESADITD
LEALRVALNKENEKAGIKLTMLAFLVKASAAALKKFPEFNASLDAAGDNLTLKKYFHIGFAADTPNGLVVPVIRDVDKKG
VLQIAQESGELAKKARDGKLGPADMSGGCFSISSLGGIGGTAFTPIINAPEVAILGVSKSAMQPVWNGKDFAPKLMLPLS
LSYDHRVIDGALAARFTTYLSQVLADMRRVLL

Sequences:

>Translated_592_residues
MAEIKEALVPDIGDYSDVPVIEVLVSVGDTVSKDQSLVTLESDKATMEVPSSVAGVVKEIKVKVGDSLSQGALVALIEVA
DAGADAAKPAAAAAPAAPAKAAPAATPAPAARAESAAPAASSDGGGLIEARVPDIGDYTDIPVIEVLVAVGDTVAKDQSL
VTLESDKATMEVPSSAAGVVKELKVKVGDTLSQGNVVAIIAAADGGAGAAQSPAKPTTDTAETAGKVEPVAVPAEPDKLA
QREIAQVQGARSGTGTPAAQGGQPSAGNPSSPPVTFDADSVLPSKVPYASPVVRVFARELGVDLNQLKGSEKGGRITRED
VQRFVKAALSGGAPAAAGAAPAGGGNGLNLLAWPKVDFSKFGETETQPLSRIKKVSGANLARNWAMIPHVTQFESADITD
LEALRVALNKENEKAGIKLTMLAFLVKASAAALKKFPEFNASLDAAGDNLTLKKYFHIGFAADTPNGLVVPVIRDVDKKG
VLQIAQESGELAKKARDGKLGPADMSGGCFSISSLGGIGGTAFTPIINAPEVAILGVSKSAMQPVWNGKDFAPKLMLPLS
LSYDHRVIDGALAARFTTYLSQVLADMRRVLL
>Mature_591_residues
AEIKEALVPDIGDYSDVPVIEVLVSVGDTVSKDQSLVTLESDKATMEVPSSVAGVVKEIKVKVGDSLSQGALVALIEVAD
AGADAAKPAAAAAPAAPAKAAPAATPAPAARAESAAPAASSDGGGLIEARVPDIGDYTDIPVIEVLVAVGDTVAKDQSLV
TLESDKATMEVPSSAAGVVKELKVKVGDTLSQGNVVAIIAAADGGAGAAQSPAKPTTDTAETAGKVEPVAVPAEPDKLAQ
REIAQVQGARSGTGTPAAQGGQPSAGNPSSPPVTFDADSVLPSKVPYASPVVRVFARELGVDLNQLKGSEKGGRITREDV
QRFVKAALSGGAPAAAGAAPAGGGNGLNLLAWPKVDFSKFGETETQPLSRIKKVSGANLARNWAMIPHVTQFESADITDL
EALRVALNKENEKAGIKLTMLAFLVKASAAALKKFPEFNASLDAAGDNLTLKKYFHIGFAADTPNGLVVPVIRDVDKKGV
LQIAQESGELAKKARDGKLGPADMSGGCFSISSLGGIGGTAFTPIINAPEVAILGVSKSAMQPVWNGKDFAPKLMLPLSL
SYDHRVIDGALAARFTTYLSQVLADMRRVLL

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 lipoyl-binding domains [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=476, Percent_Identity=28.1512605042017, Blast_Score=159, Evalue=9e-39,
Organism=Homo sapiens, GI31711992, Length=337, Percent_Identity=33.5311572700297, Blast_Score=147, Evalue=3e-35,
Organism=Homo sapiens, GI19923748, Length=229, Percent_Identity=36.2445414847162, Blast_Score=132, Evalue=7e-31,
Organism=Homo sapiens, GI203098816, Length=304, Percent_Identity=31.25, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI203098753, Length=290, Percent_Identity=31.7241379310345, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI260898739, Length=154, Percent_Identity=37.6623376623377, Blast_Score=91, Evalue=2e-18,
Organism=Escherichia coli, GI1786305, Length=308, Percent_Identity=58.4415584415584, Blast_Score=384, Evalue=1e-108,
Organism=Escherichia coli, GI1786946, Length=445, Percent_Identity=31.685393258427, Blast_Score=176, Evalue=3e-45,
Organism=Caenorhabditis elegans, GI17537937, Length=457, Percent_Identity=27.3522975929978, Blast_Score=155, Evalue=6e-38,
Organism=Caenorhabditis elegans, GI17560088, Length=448, Percent_Identity=28.7946428571429, Blast_Score=132, Evalue=7e-31,
Organism=Caenorhabditis elegans, GI25146366, Length=214, Percent_Identity=39.7196261682243, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17538894, Length=305, Percent_Identity=30.4918032786885, Blast_Score=110, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6320352, Length=238, Percent_Identity=34.8739495798319, Blast_Score=141, Evalue=3e-34,
Organism=Saccharomyces cerevisiae, GI6324258, Length=357, Percent_Identity=29.1316526610644, Blast_Score=122, Evalue=2e-28,
Organism=Drosophila melanogaster, GI18859875, Length=308, Percent_Identity=33.4415584415584, Blast_Score=158, Evalue=1e-38,
Organism=Drosophila melanogaster, GI24582497, Length=233, Percent_Identity=33.4763948497854, Blast_Score=130, Evalue=3e-30,
Organism=Drosophila melanogaster, GI20129315, Length=227, Percent_Identity=33.920704845815, Blast_Score=130, Evalue=3e-30,
Organism=Drosophila melanogaster, GI24645909, Length=209, Percent_Identity=34.9282296650718, Blast_Score=112, Evalue=5e-25,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006256
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 60390; Mature: 60259

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEIKEALVPDIGDYSDVPVIEVLVSVGDTVSKDQSLVTLESDKATMEVPSSVAGVVKEI
CCCHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEEECCCCCHHCCHHHHHHHHHH
KVKVGDSLSQGALVALIEVADAGADAAKPAAAAAPAAPAKAAPAATPAPAARAESAAPAA
HHHHCCCCCCCCEEEEEEHHCCCCCCCCCCHHCCCCCCCCCCCCCCCCCHHHCCCCCCCC
SSDGGGLIEARVPDIGDYTDIPVIEVLVAVGDTVAKDQSLVTLESDKATMEVPSSAAGVV
CCCCCCEEEEECCCCCCCCCCHHHHHHHHHCCHHHCCCCEEEEECCCCEEECCCHHHHHH
KELKVKVGDTLSQGNVVAIIAAADGGAGAAQSPAKPTTDTAETAGKVEPVAVPAEPDKLA
HHHHHHHCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHCCCCCEEECCCCHHHHH
QREIAQVQGARSGTGTPAAQGGQPSAGNPSSPPVTFDADSVLPSKVPYASPVVRVFAREL
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHH
GVDLNQLKGSEKGGRITREDVQRFVKAALSGGAPAAAGAAPAGGGNGLNLLAWPKVDFSK
CCCHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCCHHH
FGETETQPLSRIKKVSGANLARNWAMIPHVTQFESADITDLEALRVALNKENEKAGIKLT
CCCCCHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCHHH
MLAFLVKASAAALKKFPEFNASLDAAGDNLTLKKYFHIGFAADTPNGLVVPVIRDVDKKG
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEEEEECCCCCCEEEHHHHCCCHHH
VLQIAQESGELAKKARDGKLGPADMSGGCFSISSLGGIGGTAFTPIINAPEVAILGVSKS
HHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEHHCCCCCCCCHHCCCCCCCCEEEEECCHH
AMQPVWNGKDFAPKLMLPLSLSYDHRVIDGALAARFTTYLSQVLADMRRVLL
HCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure 
AEIKEALVPDIGDYSDVPVIEVLVSVGDTVSKDQSLVTLESDKATMEVPSSVAGVVKEI
CCHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEEECCCCCHHCCHHHHHHHHHH
KVKVGDSLSQGALVALIEVADAGADAAKPAAAAAPAAPAKAAPAATPAPAARAESAAPAA
HHHHCCCCCCCCEEEEEEHHCCCCCCCCCCHHCCCCCCCCCCCCCCCCCHHHCCCCCCCC
SSDGGGLIEARVPDIGDYTDIPVIEVLVAVGDTVAKDQSLVTLESDKATMEVPSSAAGVV
CCCCCCEEEEECCCCCCCCCCHHHHHHHHHCCHHHCCCCEEEEECCCCEEECCCHHHHHH
KELKVKVGDTLSQGNVVAIIAAADGGAGAAQSPAKPTTDTAETAGKVEPVAVPAEPDKLA
HHHHHHHCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHCCCCCEEECCCCHHHHH
QREIAQVQGARSGTGTPAAQGGQPSAGNPSSPPVTFDADSVLPSKVPYASPVVRVFAREL
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHH
GVDLNQLKGSEKGGRITREDVQRFVKAALSGGAPAAAGAAPAGGGNGLNLLAWPKVDFSK
CCCHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCCHHH
FGETETQPLSRIKKVSGANLARNWAMIPHVTQFESADITDLEALRVALNKENEKAGIKLT
CCCCCHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCHHH
MLAFLVKASAAALKKFPEFNASLDAAGDNLTLKKYFHIGFAADTPNGLVVPVIRDVDKKG
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEEEEECCCCCCEEEHHHHCCCHHH
VLQIAQESGELAKKARDGKLGPADMSGGCFSISSLGGIGGTAFTPIINAPEVAILGVSKS
HHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEHHCCCCCCCCHHCCCCCCCCEEEEECCHH
AMQPVWNGKDFAPKLMLPLSLSYDHRVIDGALAARFTTYLSQVLADMRRVLL
HCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8021225 [H]