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The map label for this gene is ipsJ [H]

Identifier: 21244302

GI number: 21244302

Start: 4242696

End: 4243328

Strand: Reverse

Name: ipsJ [H]

Synonym: XAC3577

Alternate gene names: 21244302

Gene position: 4243328-4242696 (Counterclockwise)

Preceding gene: 21244303

Following gene: 21244299

Centisome position: 81.99

GC content: 63.98

Gene sequence:

>633_bases
GTGATGGCCTGGACCAGAGACCAGATGGCCGCACGCGCGGCACGCGAACTGACCGATGGCGCCTACGTCAACCTGGGCAT
CGGCTTGCCGACGCTGGTCGCCAACCACATCCCCGACGGCGTCGATGTGTGGCTGCAGTCGGAAAATGGCCTGCTTGGCA
TCGGCCCGTTTCCGACCGAAGACGAAGTGGACGCGGACCTGATCAATGCAGGCAAGCAGACCGTGACCGCCCGCGCCGGT
GCCAGCTATTTCGGGAGCCACGATTCGTTCGCGATGATCCGTGGCGGCCACATCGACCTGGCGATTCTCGGCGCGATGCA
GGTCACCGAGCGTGGCGACCTGGCCAACTGGATGGTGCCCGGCAAGATGGTCAAGGGCATGGGCGGCGCAATGGATCTGG
TGGCCGGCGTCAAGCGCGTGGTGGTGTTGATGGGGCACGTCGCCAAGGATGGAAGCCACAAGATCTTGCGCGAATGCGAC
CTGCCGCTGACCGGCGTCGGCGTGGTGGACCGCATCATTACCGATCTGGCGGTGTTCGATGTCACAACGGATGGCTTGGT
GCTGGTGGAGGCGGCCGAGGGTGTGGCGCTGGATGAGTTGCGCCATAAGACGGGTGTTGAATTCGCTACTTGA

Upstream 100 bases:

>100_bases
CGGATCAGGTGCATCTGCCCGGCATTTACGTGGACCGCCTGGTGCTCAACGCCACGCCGGAAAAACGCATCGAACAGCGC
ACCGTGCGCGAAGGACAGCA

Downstream 100 bases:

>100_bases
CCGAAGGCGATCTACGCAGCGGCGAGCTCATGAGCACCTAGCGACACGCACGGCGCCTCAGGGCGCCTTACGTGCGTAGC
GGTAAGGTCCGCTGGCGGTA

Product: IpsJ protein

Products: NA

Alternate protein names: OXCT B; Succinyl CoA:3-oxoacid CoA-transferase [H]

Number of amino acids: Translated: 210; Mature: 210

Protein sequence:

>210_residues
MMAWTRDQMAARAARELTDGAYVNLGIGLPTLVANHIPDGVDVWLQSENGLLGIGPFPTEDEVDADLINAGKQTVTARAG
ASYFGSHDSFAMIRGGHIDLAILGAMQVTERGDLANWMVPGKMVKGMGGAMDLVAGVKRVVVLMGHVAKDGSHKILRECD
LPLTGVGVVDRIITDLAVFDVTTDGLVLVEAAEGVALDELRHKTGVEFAT

Sequences:

>Translated_210_residues
MMAWTRDQMAARAARELTDGAYVNLGIGLPTLVANHIPDGVDVWLQSENGLLGIGPFPTEDEVDADLINAGKQTVTARAG
ASYFGSHDSFAMIRGGHIDLAILGAMQVTERGDLANWMVPGKMVKGMGGAMDLVAGVKRVVVLMGHVAKDGSHKILRECD
LPLTGVGVVDRIITDLAVFDVTTDGLVLVEAAEGVALDELRHKTGVEFAT
>Mature_210_residues
MMAWTRDQMAARAARELTDGAYVNLGIGLPTLVANHIPDGVDVWLQSENGLLGIGPFPTEDEVDADLINAGKQTVTARAG
ASYFGSHDSFAMIRGGHIDLAILGAMQVTERGDLANWMVPGKMVKGMGGAMDLVAGVKRVVVLMGHVAKDGSHKILRECD
LPLTGVGVVDRIITDLAVFDVTTDGLVLVEAAEGVALDELRHKTGVEFAT

Specific function: SHORT-CHAIN FATTY ACIDS METABOLISM. [C]

COG id: COG2057

COG function: function code I; Acyl CoA:acetate/3-ketoacid CoA transferase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-oxoacid CoA-transferase subunit B family [H]

Homologues:

Organism=Homo sapiens, GI4557817, Length=206, Percent_Identity=60.1941747572816, Blast_Score=252, Evalue=2e-67,
Organism=Homo sapiens, GI11545841, Length=206, Percent_Identity=61.1650485436893, Blast_Score=247, Evalue=6e-66,
Organism=Escherichia coli, GI1788552, Length=203, Percent_Identity=53.2019704433498, Blast_Score=210, Evalue=7e-56,
Organism=Caenorhabditis elegans, GI17531517, Length=206, Percent_Identity=58.252427184466, Blast_Score=251, Evalue=2e-67,
Organism=Drosophila melanogaster, GI21358325, Length=205, Percent_Identity=61.9512195121951, Blast_Score=260, Evalue=4e-70,
Organism=Drosophila melanogaster, GI24655794, Length=205, Percent_Identity=61.9512195121951, Blast_Score=260, Evalue=5e-70,

Paralogues:

None

Copy number: 369 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012791
- InterPro:   IPR004165
- InterPro:   IPR004164 [H]

Pfam domain/function: PF01144 CoA_trans [H]

EC number: =2.8.3.5 [H]

Molecular weight: Translated: 22191; Mature: 22191

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS01274 COA_TRANSF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMAWTRDQMAARAARELTDGAYVNLGIGLPTLVANHIPDGVDVWLQSENGLLGIGPFPTE
CCCCCHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHCCCCEEEEEECCCCEEEECCCCCC
DEVDADLINAGKQTVTARAGASYFGSHDSFAMIRGGHIDLAILGAMQVTERGDLANWMVP
CCCCHHHHHCCCCEEEECCCCHHCCCCCCEEEEECCCEEEEEECHHHHCCCCCCCCCCCC
GKMVKGMGGAMDLVAGVKRVVVLMGHVAKDGSHKILRECDLPLTGVGVVDRIITDLAVFD
HHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHEE
VTTDGLVLVEAAEGVALDELRHKTGVEFAT
ECCCCEEEEECCCCCHHHHHHHHCCCCCCC
>Mature Secondary Structure
MMAWTRDQMAARAARELTDGAYVNLGIGLPTLVANHIPDGVDVWLQSENGLLGIGPFPTE
CCCCCHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHCCCCEEEEEECCCCEEEECCCCCC
DEVDADLINAGKQTVTARAGASYFGSHDSFAMIRGGHIDLAILGAMQVTERGDLANWMVP
CCCCHHHHHCCCCEEEECCCCHHCCCCCCEEEEECCCEEEEEECHHHHCCCCCCCCCCCC
GKMVKGMGGAMDLVAGVKRVVVLMGHVAKDGSHKILRECDLPLTGVGVVDRIITDLAVFD
HHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHEE
VTTDGLVLVEAAEGVALDELRHKTGVEFAT
ECCCCEEEEECCCCCHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9385134 [H]