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The map label for this gene is thiE

Identifier: 21244140

GI number: 21244140

Start: 4019676

End: 4020314

Strand: Direct

Name: thiE

Synonym: XAC3415

Alternate gene names: 21244140

Gene position: 4019676-4020314 (Clockwise)

Preceding gene: 21244138

Following gene: 21244141

Centisome position: 77.67

GC content: 69.48

Gene sequence:

>639_bases
ATGCCCAACCGTCCTAACGTTCGCGGCGTCTACCTGATCACCCCCGACGAGCCCAACACGCAGCGCTTGCTCCTGCGCAC
GGCCCCGTTGCTGGGGTCGATCACTTGGCTGCAATACCGCAACAAGCAGGCGGATGCGGCGCTGCGGCTGCGCCAGGCCG
GCGCCTTGCGCGAGGCATGCGCGGCCCATGGCGTGCCGTTGATCATCAACGACGATGCGCAGCTGGCGGCGCAGGTCGGT
GCGCAAGGCGTGCATCTGGGCGAAGACGATGGCGACGTGGCGGCAGCGCGCGCGCTGCTGGGCGAGCAGGCCATCATCGG
GGTGTCGTGCTACGACGATATCGAGCGCGGGCGCGCAGCGGCGGCGTCCGGGGCGAGCTATGTCGCGTTCGGTGCGTTCT
TCCCCACCACCACCAAGCAGACCACGCGGCGCGCAACGCCTGCGCTGCTACAGCAAGCGGCCGAGCTTGACGTGCCGCGC
GTGGCGATCGGCGGCATCGCACCAGCGCAGGTGCCTGCGCTGGTGACTGCGGGTGCCGATCTCATTGCGGTGGTCAGTGG
CGTGTACGCGGCGCCGGATCCGGTCGCAGCGGTACAGGGGTATCGCGCAGGGTTTGCGGCTCAGCGCGGCAAGCTTTGA

Upstream 100 bases:

>100_bases
CGGTAGGTGGTCGTGGCTGTCTCGCTCATCGGATAATCGCGGCCATGCAACAGTGGGCCTGCATTGTCCCATCCCATCCA
CCGCTTTCGGAAGCGTTGCC

Downstream 100 bases:

>100_bases
AAGTTGTTCATCCAAGCGCTTCAATCGATTTAAAGACAGCGTGTTTTTCATTTCGCTGCATCGCTTGGGTGAACAGCCGG
GTGCGTCGCGCGCGCGGTGC

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase

Number of amino acids: Translated: 212; Mature: 211

Protein sequence:

>212_residues
MPNRPNVRGVYLITPDEPNTQRLLLRTAPLLGSITWLQYRNKQADAALRLRQAGALREACAAHGVPLIINDDAQLAAQVG
AQGVHLGEDDGDVAAARALLGEQAIIGVSCYDDIERGRAAAASGASYVAFGAFFPTTTKQTTRRATPALLQQAAELDVPR
VAIGGIAPAQVPALVTAGADLIAVVSGVYAAPDPVAAVQGYRAGFAAQRGKL

Sequences:

>Translated_212_residues
MPNRPNVRGVYLITPDEPNTQRLLLRTAPLLGSITWLQYRNKQADAALRLRQAGALREACAAHGVPLIINDDAQLAAQVG
AQGVHLGEDDGDVAAARALLGEQAIIGVSCYDDIERGRAAAASGASYVAFGAFFPTTTKQTTRRATPALLQQAAELDVPR
VAIGGIAPAQVPALVTAGADLIAVVSGVYAAPDPVAAVQGYRAGFAAQRGKL
>Mature_211_residues
PNRPNVRGVYLITPDEPNTQRLLLRTAPLLGSITWLQYRNKQADAALRLRQAGALREACAAHGVPLIINDDAQLAAQVGA
QGVHLGEDDGDVAAARALLGEQAIIGVSCYDDIERGRAAAASGASYVAFGAFFPTTTKQTTRRATPALLQQAAELDVPRV
AIGGIAPAQVPALVTAGADLIAVVSGVYAAPDPVAAVQGYRAGFAAQRGKL

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP)

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family

Homologues:

Organism=Escherichia coli, GI1790426, Length=181, Percent_Identity=35.3591160220994, Blast_Score=78, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6325042, Length=222, Percent_Identity=28.8288288288288, Blast_Score=82, Evalue=7e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIE_XANAC (Q8PH45)

Other databases:

- EMBL:   AE008923
- RefSeq:   NP_643722.1
- ProteinModelPortal:   Q8PH45
- GeneID:   1157486
- GenomeReviews:   AE008923_GR
- KEGG:   xac:XAC3415
- NMPDR:   fig|190486.1.peg.3366
- HOGENOM:   HBG754477
- OMA:   SAICHAE
- ProtClustDB:   PRK00043
- BioCyc:   XAXO190486:XAC3415-MONOMER
- BRENDA:   2.5.1.3
- HAMAP:   MF_00097
- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00693

Pfam domain/function: PF02581 TMP-TENI; SSF51391 TMP_synthase

EC number: =2.5.1.3

Molecular weight: Translated: 21988; Mature: 21857

Theoretical pI: Translated: 8.20; Mature: 8.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNRPNVRGVYLITPDEPNTQRLLLRTAPLLGSITWLQYRNKQADAALRLRQAGALREAC
CCCCCCCCEEEEECCCCCCHHEEHEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
AAHGVPLIINDDAQLAAQVGAQGVHLGEDDGDVAAARALLGEQAIIGVSCYDDIERGRAA
HHCCCCEEECCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCEEEEEEHHHHHHHHHHH
AASGASYVAFGAFFPTTTKQTTRRATPALLQQAAELDVPRVAIGGIAPAQVPALVTAGAD
HCCCCCEEEEEEECCCCCCHHHHHCCHHHHHHHHHCCCCEEEECCCCHHHHHHHHHCCHH
LIAVVSGVYAAPDPVAAVQGYRAGFAAQRGKL
HHHHHHCCCCCCCCHHHHHHHHHCCCHHCCCC
>Mature Secondary Structure 
PNRPNVRGVYLITPDEPNTQRLLLRTAPLLGSITWLQYRNKQADAALRLRQAGALREAC
CCCCCCCEEEEECCCCCCHHEEHEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
AAHGVPLIINDDAQLAAQVGAQGVHLGEDDGDVAAARALLGEQAIIGVSCYDDIERGRAA
HHCCCCEEECCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCEEEEEEHHHHHHHHHHH
AASGASYVAFGAFFPTTTKQTTRRATPALLQQAAELDVPRVAIGGIAPAQVPALVTAGAD
HCCCCCEEEEEEECCCCCCHHHHHCCHHHHHHHHHCCCCEEEECCCCHHHHHHHHHCCHH
LIAVVSGVYAAPDPVAAVQGYRAGFAAQRGKL
HHHHHHCCCCCCCCHHHHHHHHHCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217