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The map label for this gene is blh [H]

Identifier: 21244082

GI number: 21244082

Start: 3954548

End: 3955384

Strand: Direct

Name: blh [H]

Synonym: XAC3357

Alternate gene names: 21244082

Gene position: 3954548-3955384 (Clockwise)

Preceding gene: 21244081

Following gene: 21244083

Centisome position: 76.41

GC content: 65.83

Gene sequence:

>837_bases
GTGGAGACGCCGATGCAGCCCCAGGTACTTGCCTTCTTTCATGCCGACAGCAACACCTTCACCTATGTGGTCGCCGATGC
GGCCACCGGTGCGGCGGCGGTGATCGACCCGGCGCTGGACTACGCGGCCGAGAGCGGCATCATCGGCAGCCAGGCGGCGC
AGGGCATCCTTACCGCCATCGAGCAACACGGATGGCAGTTGCAGTGGCTGCTGGAAACCCATGCGCATGCCGATCACCTG
TCTGCGGCGCAGTGGCTGAAGCAGCACTGGCCGCAGGCGCGCATCGGCATCGGCGAGGGAATTTGCCAAGTGCAACGAAC
CCTTGCCCCGCAGTACGCATTGCCGGACGACTTCCGTGCCGATGGCTCGCAATTCGATCATCTGTTCGCCGATGACGAGC
GCTTCAAGCTTGGCGGTATCGAAGCACGCGTCATCGCGGTGCCCGGCCACACCAGCGACAGCATTGCGTATCTGATCGGC
GATGCGCTGTTTCCTGGCGACTCGTTGTTCATGCCGGATGCGGGCACGGCGCGTTGCGATTTCCCGGGCGGCAATGCCCG
CCAGCTGTTTGCGTCGATCCAGCGCCTGTACGCGCTGCCGGAGAGCACCCGCGTGTTCGTCTGCCACGACTATGGCCCGG
GCGGGCGCGCGGTGGCGCATCAAACCAGTATCGGCGCGCAGCGGCGAAGCAACATCCACGTACGCGACGGCGTGGACGTG
GAGACCTTCGTGGCGCAGCGGCAGGCGCGCGATGCCACCTTGCCCGAACCCAAGTTGATCCGCCCGGCCCTGCAAACCAA
TCTTCAGGCCGGGCGCTGGGCCGGAGTGGTCCTGTAG

Upstream 100 bases:

>100_bases
ACCTACATCGCCACCTATCGCCCGATCGCCGAAACCCAGCTACGCATCGCAGGTGACCGGCTAGCCGCCATCCTCAACGC
GGCACTGGCCAGCCCCTGAC

Downstream 100 bases:

>100_bases
CGCGCGCCGGCTCGCCACGCGGTGCGCATCGCGTAGCCGCTATGATCGGGGCCCTGTCCTGGTGAGGAGCCTTCGATGCG
CATGATCGGTTTCTGGCAGC

Product: beta-lactamase

Products: Reduced glutathione; H+; D-Lactate [C]

Alternate protein names: NA

Number of amino acids: Translated: 278; Mature: 278

Protein sequence:

>278_residues
METPMQPQVLAFFHADSNTFTYVVADAATGAAAVIDPALDYAAESGIIGSQAAQGILTAIEQHGWQLQWLLETHAHADHL
SAAQWLKQHWPQARIGIGEGICQVQRTLAPQYALPDDFRADGSQFDHLFADDERFKLGGIEARVIAVPGHTSDSIAYLIG
DALFPGDSLFMPDAGTARCDFPGGNARQLFASIQRLYALPESTRVFVCHDYGPGGRAVAHQTSIGAQRRSNIHVRDGVDV
ETFVAQRQARDATLPEPKLIRPALQTNLQAGRWAGVVL

Sequences:

>Translated_278_residues
METPMQPQVLAFFHADSNTFTYVVADAATGAAAVIDPALDYAAESGIIGSQAAQGILTAIEQHGWQLQWLLETHAHADHL
SAAQWLKQHWPQARIGIGEGICQVQRTLAPQYALPDDFRADGSQFDHLFADDERFKLGGIEARVIAVPGHTSDSIAYLIG
DALFPGDSLFMPDAGTARCDFPGGNARQLFASIQRLYALPESTRVFVCHDYGPGGRAVAHQTSIGAQRRSNIHVRDGVDV
ETFVAQRQARDATLPEPKLIRPALQTNLQAGRWAGVVL
>Mature_278_residues
METPMQPQVLAFFHADSNTFTYVVADAATGAAAVIDPALDYAAESGIIGSQAAQGILTAIEQHGWQLQWLLETHAHADHL
SAAQWLKQHWPQARIGIGEGICQVQRTLAPQYALPDDFRADGSQFDHLFADDERFKLGGIEARVIAVPGHTSDSIAYLIG
DALFPGDSLFMPDAGTARCDFPGGNARQLFASIQRLYALPESTRVFVCHDYGPGGRAVAHQTSIGAQRRSNIHVRDGVDV
ETFVAQRQARDATLPEPKLIRPALQTNLQAGRWAGVVL

Specific function: Thiolesterase That Catalyzes The Hydrolysis Of S-D- Lactoyl-Glutathione To Form Glutathione And D-Lactic Acid. [C]

COG id: COG0491

COG function: function code R; Zn-dependent hydrolases, including glyoxylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-B beta-lactamase family [H]

Homologues:

Organism=Homo sapiens, GI41327741, Length=264, Percent_Identity=25, Blast_Score=85, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI17538952, Length=272, Percent_Identity=26.1029411764706, Blast_Score=88, Evalue=4e-18,
Organism=Drosophila melanogaster, GI221330176, Length=275, Percent_Identity=26.9090909090909, Blast_Score=107, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005939
- InterPro:   IPR001279 [H]

Pfam domain/function: PF04273 DUF442; PF00753 Lactamase_B [H]

EC number: 3.1.2.6 [C]

Molecular weight: Translated: 30161; Mature: 30161

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METPMQPQVLAFFHADSNTFTYVVADAATGAAAVIDPALDYAAESGIIGSQAAQGILTAI
CCCCCCCCEEEEEECCCCEEEEEEEECCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
EQHGWQLQWLLETHAHADHLSAAQWLKQHWPQARIGIGEGICQVQRTLAPQYALPDDFRA
HHCCCEEEEEEHHHCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCC
DGSQFDHLFADDERFKLGGIEARVIAVPGHTSDSIAYLIGDALFPGDSLFMPDAGTARCD
CCCCCCCCCCCCCEEEECCCEEEEEEECCCCCCCHHEEEHHHCCCCCCEECCCCCCCEEC
FPGGNARQLFASIQRLYALPESTRVFVCHDYGPGGRAVAHQTSIGAQRRSNIHVRDGVDV
CCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCEEEEHHHCCHHHHCCEEEECCCCH
ETFVAQRQARDATLPEPKLIRPALQTNLQAGRWAGVVL
HHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCEEEECC
>Mature Secondary Structure
METPMQPQVLAFFHADSNTFTYVVADAATGAAAVIDPALDYAAESGIIGSQAAQGILTAI
CCCCCCCCEEEEEECCCCEEEEEEEECCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
EQHGWQLQWLLETHAHADHLSAAQWLKQHWPQARIGIGEGICQVQRTLAPQYALPDDFRA
HHCCCEEEEEEHHHCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCC
DGSQFDHLFADDERFKLGGIEARVIAVPGHTSDSIAYLIGDALFPGDSLFMPDAGTARCD
CCCCCCCCCCCCCEEEECCCEEEEEEECCCCCCCHHEEEHHHCCCCCCEECCCCCCCEEC
FPGGNARQLFASIQRLYALPESTRVFVCHDYGPGGRAVAHQTSIGAQRRSNIHVRDGVDV
CCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCEEEEHHHCCHHHHCCEEEECCCCH
ETFVAQRQARDATLPEPKLIRPALQTNLQAGRWAGVVL
HHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: H2O; (R)-S-Lactoylglutathione [C]

Specific reaction: H2O + (R)-S-Lactoylglutathione --> Reduced glutathione + H+ + D-Lactate [C]

General reaction: Thioester hydrolysis [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194 [H]