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The map label for this gene is barA [H]

Identifier: 21244061

GI number: 21244061

Start: 3931798

End: 3933159

Strand: Direct

Name: barA [H]

Synonym: XAC3335

Alternate gene names: 21244061

Gene position: 3931798-3933159 (Clockwise)

Preceding gene: 21244057

Following gene: 21244065

Centisome position: 75.97

GC content: 66.08

Gene sequence:

>1362_bases
ATGGGCCAGCCGCTGCAGCCCCAAGCCCGTTTACCGCACGCCGGCCTCGTCCACGCGCCGCCGTCCTGGTCGGCAGTCAC
AGTTTCGTCCGACCTCTGTGACCGCAGACACATGAAGGGCCTTGGCATGCCCTATGCTTGCCGCTTCCACCCTGTAGCTA
CGCGCATGAGTACCGTCCTGTCCGATCGAGCGGCCGAGCCGCTTCCCTGCGCACCCAAGCCCCCCAGCGAAGCATTGCGT
CTGCAGGCGCTGCGCAGCTACGGCATTCTCGACACCCCGCGCGAAGCGGCCTTCGAAGACATCACCCGGCTTGCCTCGAT
CATTTGCCAGACGCCGATCGCGCTGATCAGCCTGGTCGATGCGGACCGCCAGTGGTTCAAGAGCGAGCTGGGGATGGGCG
AGCGCGAGACCCCGCTGTTCAAGTCGATGTGCGCGCATGCGCTGCTCGACAACGATGTGCTGGTGGTGCCCGACACGCGC
GAAGACATCCGCTTTGCGCGCAACCCACTGGTCACCGGCGAGGCGCCGCTGCATTTCTATGCAGGTGCCTTGTTGAAGAC
ACCAGACGGCCTGCCGCTTGGCACGGTCTGCGTGCTCGACCGGAAACCGCGCCAGCTCACCGACGAGCAGGTGGAAGCCC
TGCGCGCATTGGCACGCCAGACCATGGCGCAGCTGGAATTTCGGCGCGCACTGGCAATCGCAGAGGAATCCGATCGCTAC
CGCAGCCGCCTGATGGCCATCGCCGGGCACGATCTGAAGACCCCATTGCGCACTGCCGCCTACGCCATCGACAAGATGCG
CCGGCACGCCGACGTCGACGCCGTTCCCAACCTGGTCGCTGCGCGCGATGCGATCAGCCTGGTCGGCCGCAGCCTGGACG
AGCTGGCCACGCTGGCGGCCGCGAGCAAGGCCGCCATGCCGGACCTGCAGCCACTGCAGCTGGAAGACGTGCTGCATTCG
ATCCTCGGCGTGTGGCGTCAACCAGCCATCGACAAGTACCTGGCCCTTCGCTATGTGCACACCTCGCTGCGCGTGCGCAG
CCACCCCACGCTGCTGACCACCTTGATCGGCAACCTGGTCGGCAATGCCATCAAGTACACCGAACGCGGCCGCGTGCTGG
TCGGCACCCGCCGCCGTCCCGGTTTTGCGGAAGTGAACATCATCGACAGCGGCATCGGCTTGAACATGGACCAACCCGAG
CAGGTCTTCCAGGCGTTCCGCCAGGCCGACCCGCGCAGCGACGGTTTGGGCATCGGCCTGTGGATCGTGCATCGCACTGC
CGAGACATTGGGCTGCGAAGTGGACGTGCGTCCGCGCCCGCAGGGCGGCACCTGTTTTTCGGTGCGGATTCCATTGGCCT
AA

Upstream 100 bases:

>100_bases
GTCGCGGCCGATGCGCGACCGCATGTCGCACCCGGCTGGCCCGGTCACACCCGGATGACCCGCGCCCAGGCATGCGCCGG
AGCCGGCACGCCAGAACCGG

Downstream 100 bases:

>100_bases
AGATGTGTGGCCCGCAGCGTGTTCGGGCTGCGCGGCGAACGCCTGGGGTTTACCGGTTGGGGCAGCGGTCTGCGGCCAGA
CCAGAACGCGCGGCCACTCC

Product: sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 453; Mature: 452

Protein sequence:

>453_residues
MGQPLQPQARLPHAGLVHAPPSWSAVTVSSDLCDRRHMKGLGMPYACRFHPVATRMSTVLSDRAAEPLPCAPKPPSEALR
LQALRSYGILDTPREAAFEDITRLASIICQTPIALISLVDADRQWFKSELGMGERETPLFKSMCAHALLDNDVLVVPDTR
EDIRFARNPLVTGEAPLHFYAGALLKTPDGLPLGTVCVLDRKPRQLTDEQVEALRALARQTMAQLEFRRALAIAEESDRY
RSRLMAIAGHDLKTPLRTAAYAIDKMRRHADVDAVPNLVAARDAISLVGRSLDELATLAAASKAAMPDLQPLQLEDVLHS
ILGVWRQPAIDKYLALRYVHTSLRVRSHPTLLTTLIGNLVGNAIKYTERGRVLVGTRRRPGFAEVNIIDSGIGLNMDQPE
QVFQAFRQADPRSDGLGIGLWIVHRTAETLGCEVDVRPRPQGGTCFSVRIPLA

Sequences:

>Translated_453_residues
MGQPLQPQARLPHAGLVHAPPSWSAVTVSSDLCDRRHMKGLGMPYACRFHPVATRMSTVLSDRAAEPLPCAPKPPSEALR
LQALRSYGILDTPREAAFEDITRLASIICQTPIALISLVDADRQWFKSELGMGERETPLFKSMCAHALLDNDVLVVPDTR
EDIRFARNPLVTGEAPLHFYAGALLKTPDGLPLGTVCVLDRKPRQLTDEQVEALRALARQTMAQLEFRRALAIAEESDRY
RSRLMAIAGHDLKTPLRTAAYAIDKMRRHADVDAVPNLVAARDAISLVGRSLDELATLAAASKAAMPDLQPLQLEDVLHS
ILGVWRQPAIDKYLALRYVHTSLRVRSHPTLLTTLIGNLVGNAIKYTERGRVLVGTRRRPGFAEVNIIDSGIGLNMDQPE
QVFQAFRQADPRSDGLGIGLWIVHRTAETLGCEVDVRPRPQGGTCFSVRIPLA
>Mature_452_residues
GQPLQPQARLPHAGLVHAPPSWSAVTVSSDLCDRRHMKGLGMPYACRFHPVATRMSTVLSDRAAEPLPCAPKPPSEALRL
QALRSYGILDTPREAAFEDITRLASIICQTPIALISLVDADRQWFKSELGMGERETPLFKSMCAHALLDNDVLVVPDTRE
DIRFARNPLVTGEAPLHFYAGALLKTPDGLPLGTVCVLDRKPRQLTDEQVEALRALARQTMAQLEFRRALAIAEESDRYR
SRLMAIAGHDLKTPLRTAAYAIDKMRRHADVDAVPNLVAARDAISLVGRSLDELATLAAASKAAMPDLQPLQLEDVLHSI
LGVWRQPAIDKYLALRYVHTSLRVRSHPTLLTTLIGNLVGNAIKYTERGRVLVGTRRRPGFAEVNIIDSGIGLNMDQPEQ
VFQAFRQADPRSDGLGIGLWIVHRTAETLGCEVDVRPRPQGGTCFSVRIPLA

Specific function: Member of the two-component regulatory system UvrY/BarA involved in the regulation of carbon metabolism via the CsrA/CsrB regulatory system. Phosphorylates UvrY, probably via a four-step phosphorelay [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1789149, Length=265, Percent_Identity=28.6792452830189, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI87081816, Length=283, Percent_Identity=26.1484098939929, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI145693157, Length=229, Percent_Identity=24.0174672489083, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011006
- InterPro:   IPR019247
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR008207
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- InterPro:   IPR001789 [H]

Pfam domain/function: PF09984 DUF2222; PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF01627 Hpt; PF00072 Response_reg [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 49881; Mature: 49750

Theoretical pI: Translated: 8.57; Mature: 8.57

Prosite motif: PS50109 HIS_KIN ; PS00136 SUBTILASE_ASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGQPLQPQARLPHAGLVHAPPSWSAVTVSSDLCDRRHMKGLGMPYACRFHPVATRMSTVL
CCCCCCCHHCCCCCCEEECCCCCCEEEECHHHHHHHHHHCCCCCCEEECCHHHHHHHHHH
SDRAAEPLPCAPKPPSEALRLQALRSYGILDTPREAAFEDITRLASIICQTPIALISLVD
HHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ADRQWFKSELGMGERETPLFKSMCAHALLDNDVLVVPDTREDIRFARNPLVTGEAPLHFY
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCEEEECCCHHHHHHHCCCCCCCCCCHHHH
AGALLKTPDGLPLGTVCVLDRKPRQLTDEQVEALRALARQTMAQLEFRRALAIAEESDRY
HHHHHCCCCCCCCCEEEEECCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RSRLMAIAGHDLKTPLRTAAYAIDKMRRHADVDAVPNLVAARDAISLVGRSLDELATLAA
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
ASKAAMPDLQPLQLEDVLHSILGVWRQPAIDKYLALRYVHTSLRVRSHPTLLTTLIGNLV
HHHHCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
GNAIKYTERGRVLVGTRRRPGFAEVNIIDSGIGLNMDQPEQVFQAFRQADPRSDGLGIGL
HHHHHCCCCCCEEEECCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCEEEE
WIVHRTAETLGCEVDVRPRPQGGTCFSVRIPLA
EEEEHHHHHHCCEEECCCCCCCCCEEEEEECCC
>Mature Secondary Structure 
GQPLQPQARLPHAGLVHAPPSWSAVTVSSDLCDRRHMKGLGMPYACRFHPVATRMSTVL
CCCCCCHHCCCCCCEEECCCCCCEEEECHHHHHHHHHHCCCCCCEEECCHHHHHHHHHH
SDRAAEPLPCAPKPPSEALRLQALRSYGILDTPREAAFEDITRLASIICQTPIALISLVD
HHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ADRQWFKSELGMGERETPLFKSMCAHALLDNDVLVVPDTREDIRFARNPLVTGEAPLHFY
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCEEEECCCHHHHHHHCCCCCCCCCCHHHH
AGALLKTPDGLPLGTVCVLDRKPRQLTDEQVEALRALARQTMAQLEFRRALAIAEESDRY
HHHHHCCCCCCCCCEEEEECCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RSRLMAIAGHDLKTPLRTAAYAIDKMRRHADVDAVPNLVAARDAISLVGRSLDELATLAA
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
ASKAAMPDLQPLQLEDVLHSILGVWRQPAIDKYLALRYVHTSLRVRSHPTLLTTLIGNLV
HHHHCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
GNAIKYTERGRVLVGTRRRPGFAEVNIIDSGIGLNMDQPEQVFQAFRQADPRSDGLGIGL
HHHHHCCCCCCEEEECCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCEEEE
WIVHRTAETLGCEVDVRPRPQGGTCFSVRIPLA
EEEEHHHHHHCCEEECCCCCCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]