Click here to switch to the map view.

The map label for this gene is colS [H]

Identifier: 21243975

GI number: 21243975

Start: 3830275

End: 3831432

Strand: Reverse

Name: colS [H]

Synonym: XAC3249

Alternate gene names: 21243975

Gene position: 3831432-3830275 (Counterclockwise)

Preceding gene: 21243976

Following gene: 21243967

Centisome position: 74.03

GC content: 65.2

Gene sequence:

>1158_bases
ATGAACCGCAACATCGATGCCTTCGCGCAACGCTTTTATTCCGATCCGATGCGCAACCCCGATCTGCCGTTGCAGCAGAT
GCGCGGGCGCGTGGTCAAAAGCGACAAGTTCGAAGCGCTACGCCGCGAACAACCGGAGTGGTACCAGCTGCCCGACGGCA
TCCACACCATCACCGGCGTCGACGAAAGCGGCACTACCTATTCGTACAAGCTTGCGGTGCGCAAAACGCCGAGCGAATGG
TTCTTCCTCGCCTACGACATGACCCAGGCGCTGAAGGGCGAAATCCAGCTCAAGCGCACCCTGGTCTTGTCGGTATTGGT
GTTCAGCGGGTTTTCGCTGCTGATCGGCTGGTGGTCCGCGTCCAAGGTGATGCGGCCGGTCTCCGATCTGGCCGCACGCC
TGCGGGCCTATCGCGGCGGCACCAGCCAACCCAAGCCACTGGCCGCGCACTTCCCGGACGACGAGGTCGGCCAGCTGGCC
GAAGCGCTGGACGACTACTCGGCGCGTCTGACCGAAGTGGTGCAGCGCGACCGCGAATTCAACGCCGACGTCAGCCACGA
ACTGCGCACCCCGCTGGCGGTGATCCGCGGCGCCACCGAGCTGCTGCTGACCAAGCCCAACCTGGACGAAAAGGTGTTGC
AGCGGCTGCAACGCATCCAGCGCGCCGAGCAGCAATGCAGCGATTTGATCGGCTCGTTGCTGTTGCTCTCGCGCAACGAG
CGCGGGCAGGGCAGCAGCAATGTCGCCAAGGTGGCCGAACAACTGATCGACTCGCACCGCGCCCAGCTCGGCGGAAAACC
CCTGGAACTCGTGCTGGAAGGCGAGCGCGATCTGGTCATCGATGCCCCGGAATCCGCCCTGTCGGTCGCGCTCGGCAACC
TGATCGGCAACGCCGTCAAATACACCCAGGACGGCCAGGTGCGTGTGCGCGTGCTGGGCGATGCAGTGGAAGTGATCGAT
TCGGGCCCCGGCCTGAGCGAAGAGGACGCCGCCAAGCTGTTCCAGCGCGGCTACCGCGGCACCCACGCCGGCCACTCGCA
AGGCGGCGGCATCGGCTTGTCGATCGTCAGCCGGCTTTGCGATCTGTACGGCTGGCGGGTCAGCGTGCGCCCAGGCCAGC
AGCGCGGCGTCGTCGCAACATTGGCCTTCCATCGCTAA

Upstream 100 bases:

>100_bases
CCTGTCGTTCGTGTTGTTGGGCTTCTGCCTGACAACGTTGTTCGCCTTCGCCACCAACTGGGCGCGCTCGCGCGTGGAAA
ACCAGCTGGTGGAAGACGTG

Downstream 100 bases:

>100_bases
CCACCACCCGCACTCCCGTAGGAGCGCACCCGGGCGCGACGGCCGTACCCACTGCGCCCCCAGGGCAGCGCCTCCAGACA
ACAACCGCACCCAAAACCCA

Product: two-component system sensor protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 385; Mature: 385

Protein sequence:

>385_residues
MNRNIDAFAQRFYSDPMRNPDLPLQQMRGRVVKSDKFEALRREQPEWYQLPDGIHTITGVDESGTTYSYKLAVRKTPSEW
FFLAYDMTQALKGEIQLKRTLVLSVLVFSGFSLLIGWWSASKVMRPVSDLAARLRAYRGGTSQPKPLAAHFPDDEVGQLA
EALDDYSARLTEVVQRDREFNADVSHELRTPLAVIRGATELLLTKPNLDEKVLQRLQRIQRAEQQCSDLIGSLLLLSRNE
RGQGSSNVAKVAEQLIDSHRAQLGGKPLELVLEGERDLVIDAPESALSVALGNLIGNAVKYTQDGQVRVRVLGDAVEVID
SGPGLSEEDAAKLFQRGYRGTHAGHSQGGGIGLSIVSRLCDLYGWRVSVRPGQQRGVVATLAFHR

Sequences:

>Translated_385_residues
MNRNIDAFAQRFYSDPMRNPDLPLQQMRGRVVKSDKFEALRREQPEWYQLPDGIHTITGVDESGTTYSYKLAVRKTPSEW
FFLAYDMTQALKGEIQLKRTLVLSVLVFSGFSLLIGWWSASKVMRPVSDLAARLRAYRGGTSQPKPLAAHFPDDEVGQLA
EALDDYSARLTEVVQRDREFNADVSHELRTPLAVIRGATELLLTKPNLDEKVLQRLQRIQRAEQQCSDLIGSLLLLSRNE
RGQGSSNVAKVAEQLIDSHRAQLGGKPLELVLEGERDLVIDAPESALSVALGNLIGNAVKYTQDGQVRVRVLGDAVEVID
SGPGLSEEDAAKLFQRGYRGTHAGHSQGGGIGLSIVSRLCDLYGWRVSVRPGQQRGVVATLAFHR
>Mature_385_residues
MNRNIDAFAQRFYSDPMRNPDLPLQQMRGRVVKSDKFEALRREQPEWYQLPDGIHTITGVDESGTTYSYKLAVRKTPSEW
FFLAYDMTQALKGEIQLKRTLVLSVLVFSGFSLLIGWWSASKVMRPVSDLAARLRAYRGGTSQPKPLAAHFPDDEVGQLA
EALDDYSARLTEVVQRDREFNADVSHELRTPLAVIRGATELLLTKPNLDEKVLQRLQRIQRAEQQCSDLIGSLLLLSRNE
RGQGSSNVAKVAEQLIDSHRAQLGGKPLELVLEGERDLVIDAPESALSVALGNLIGNAVKYTQDGQVRVRVLGDAVEVID
SGPGLSEEDAAKLFQRGYRGTHAGHSQGGGIGLSIVSRLCDLYGWRVSVRPGQQRGVVATLAFHR

Specific function: Member of a two-component regulatory system qseB/qseC. May activate qseB by phosphorylation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1790861, Length=304, Percent_Identity=27.9605263157895, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1790551, Length=275, Percent_Identity=29.4545454545455, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1789403, Length=268, Percent_Identity=30.9701492537313, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI1786783, Length=231, Percent_Identity=28.5714285714286, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1788393, Length=227, Percent_Identity=27.7533039647577, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1786600, Length=204, Percent_Identity=31.3725490196078, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1789149, Length=352, Percent_Identity=27.8409090909091, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI48994928, Length=231, Percent_Identity=31.6017316017316, Blast_Score=75, Evalue=9e-15,
Organism=Escherichia coli, GI1788279, Length=219, Percent_Identity=26.4840182648402, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1788713, Length=223, Percent_Identity=29.5964125560538, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1790346, Length=196, Percent_Identity=31.1224489795918, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI145693157, Length=233, Percent_Identity=27.0386266094421, Blast_Score=65, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 42716; Mature: 42716

Theoretical pI: Translated: 8.21; Mature: 8.21

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRNIDAFAQRFYSDPMRNPDLPLQQMRGRVVKSDKFEALRREQPEWYQLPDGIHTITGV
CCCCHHHHHHHHHCCCCCCCCCCHHHHCCCHHCCHHHHHHHCCCCCCEECCCCCEEEECC
DESGTTYSYKLAVRKTPSEWFFLAYDMTQALKGEIQLKRTLVLSVLVFSGFSLLIGWWSA
CCCCCEEEEEEEEEECCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
SKVMRPVSDLAARLRAYRGGTSQPKPLAAHFPDDEVGQLAEALDDYSARLTEVVQRDREF
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
NADVSHELRTPLAVIRGATELLLTKPNLDEKVLQRLQRIQRAEQQCSDLIGSLLLLSRNE
CCCHHHHHHHHHHHHHCCHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
RGQGSSNVAKVAEQLIDSHRAQLGGKPLELVLEGERDLVIDAPESALSVALGNLIGNAVK
CCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEECCHHHHHHHHHHHHHHHHE
YTQDGQVRVRVLGDAVEVIDSGPGLSEEDAAKLFQRGYRGTHAGHSQGGGIGLSIVSRLC
ECCCCCEEEEEEHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHH
DLYGWRVSVRPGQQRGVVATLAFHR
HHHCCEEEECCCCCCCEEEEEEECC
>Mature Secondary Structure
MNRNIDAFAQRFYSDPMRNPDLPLQQMRGRVVKSDKFEALRREQPEWYQLPDGIHTITGV
CCCCHHHHHHHHHCCCCCCCCCCHHHHCCCHHCCHHHHHHHCCCCCCEECCCCCEEEECC
DESGTTYSYKLAVRKTPSEWFFLAYDMTQALKGEIQLKRTLVLSVLVFSGFSLLIGWWSA
CCCCCEEEEEEEEEECCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
SKVMRPVSDLAARLRAYRGGTSQPKPLAAHFPDDEVGQLAEALDDYSARLTEVVQRDREF
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
NADVSHELRTPLAVIRGATELLLTKPNLDEKVLQRLQRIQRAEQQCSDLIGSLLLLSRNE
CCCHHHHHHHHHHHHHCCHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
RGQGSSNVAKVAEQLIDSHRAQLGGKPLELVLEGERDLVIDAPESALSVALGNLIGNAVK
CCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEECCHHHHHHHHHHHHHHHHE
YTQDGQVRVRVLGDAVEVIDSGPGLSEEDAAKLFQRGYRGTHAGHSQGGGIGLSIVSRLC
ECCCCCEEEEEEHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHH
DLYGWRVSVRPGQQRGVVATLAFHR
HHHCCEEEECCCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023 [H]