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The map label for this gene is nadE [H]

Identifier: 21243946

GI number: 21243946

Start: 3793856

End: 3795496

Strand: Reverse

Name: nadE [H]

Synonym: XAC3220

Alternate gene names: 21243946

Gene position: 3795496-3793856 (Counterclockwise)

Preceding gene: 21243947

Following gene: 21243943

Centisome position: 73.34

GC content: 66.18

Gene sequence:

>1641_bases
ATGTCCCAGACCCTCCGCATCGCCATGGCCCAGTTCGACTTCCCGGTCGGCGGCGTAGCGCAGAACACCGATCGCATCAT
CGAGTACATCGCAGCGGCGCGCGACGAGTTCGAGGCCGACATCGTGCTGTTTCCCGAGTTGGCGATCAGCGGGTATCCAC
CGGAAGACTTGCTGTTAAGGCCGGGATTCCTCGCTCATTGCGAACAGGCGCTGGCACGGATTGCGGCCGCCACGCGCGGC
ATCGTGGCAGTGGTCGGCTGGCCGCAAAGCGCGGGCAGCGTGGTCTACAACGCCGCCAGCGTGTTGCGCGACGGGCGGAT
CGAAGCCACCTACCGCAAGCGCGAATTGCCCAACTATGCGGTGTTCGACGAGCGTCGTTATTTCGACGTGGACCCGGATG
GCGAGAACTGCGTGGTCACGGTCAAGGGCGTGCGGGTGGGCGTGGTGATCTGCGAAGACCTCTGGTTCGCCGAGCCGTTG
GCCAACACGGTACGGGCGGGCGCGGAGTTGGTGCTGGTGCCTAACGCCTCGCCCTACGAGCGCGGCAAGCATGCGCAACG
CGATGCATTGCTGGCCGAACGCACCCGCGAGAGCGGGGCGGCGATTGCCTATCTCAACGTCGTGGGCGGGCAGGATGCAC
TGGTGTTCGATGGCGCCTCGGTGGTGGCCGATGGCGACGGCACCGTGCACCCCGCCGCTGCAGCCTTCGTGGACCAGTGG
CTGGTGGTGGACTACGCGGCCGGCGAGCGCAGCTTCACTCCGGTGGTGTGGGTGGATGACGGCGACGAGAGCATGGACGC
GCTGGCCTGGCGCGCGGTGGTGCGCGGGCTGCAGGACTATTGCCGCAAGAACGGCTTCAGCAAGGTCTGGCTGGGCTTGT
CTGGCGGCATCGACTCGGCACTGGTGTTGGCGATGGCGGTGGATGCGCTGGGCGGCGACAACGTGACCGCCGTGCGGTTG
CCGTCGCGCTACACCGCCAATCTGTCCAACGACCTGGCCGACGAGCAATGTCGCGCGCTGGGCGTGAAGCTGGAGACCAT
CGCGATCGAGCCGGCTTTCGAAGGCCTGCTCGGCGCGCTGGGGCCGATGTTCGAAGGCACACAGCCGGACATCACCGAAG
AAAACCTGCAGTCGCGCAGCCGTGGCGTGATCCTGATGGCGCTGTCGAACAAGTTCGGCGGACTGGTGCTGACCACCGGT
AACAAGAGCGAATACGCAGTGGGTTACGCCACCATCTATGGCGACATGTGCGGTGGCTACGCGCCGCTCAAGGACCTGTA
CAAGACGGAGGTGTTCGGCCTGGCGAAATGGCGCAACACCGTCGGCGGCGCGCCGGTGATTCCGCCAGCGGTGATCTCGC
GCCCGCCGTCGGCCGAGTTGCGCGACAACCAGACCGACCAGGATTCGCTGCCGCCGTACGATGTGCTCGACGGCATCCTG
TATCGCTACGTCGATCAGGAGCAGTCGCGCGACGACATCGTTGCGGCAGGCTACGCGGCCGATACCGTCGAGCATGTGCT
GCGCCTGGTACGGCTCAACGAGTGGAAGCGCCATCAGTCCGCGCCCGGGCCCAAGGTGTCGCGGCGTGCATTCGGTCGCG
AACGGCGCTATCCGATCACCAACGGATATCGCGGGCAGTAG

Upstream 100 bases:

>100_bases
ACCGATGCTGTCTGCGACGCGATTGTGCAACGGCTGTGCGTTGCCCGCTTTTCTCCTCCTCCTTCCGGCGTTCGGTAGGA
ATCTGCAAAAGGCTGCCGAT

Downstream 100 bases:

>100_bases
TGCGAACGGCTAATCCGGTCACTCGGCTGACGCAGTGGCTGCCGCTGCGGTGGCCGGCGGCTGGGCGAACCGCGTGAGCA
GATCCAGGGGCGGTTTTGGT

Product: NAD synthetase

Products: NA

Alternate protein names: NAD(+) synthase [glutamine-hydrolysing] [H]

Number of amino acids: Translated: 546; Mature: 545

Protein sequence:

>546_residues
MSQTLRIAMAQFDFPVGGVAQNTDRIIEYIAAARDEFEADIVLFPELAISGYPPEDLLLRPGFLAHCEQALARIAAATRG
IVAVVGWPQSAGSVVYNAASVLRDGRIEATYRKRELPNYAVFDERRYFDVDPDGENCVVTVKGVRVGVVICEDLWFAEPL
ANTVRAGAELVLVPNASPYERGKHAQRDALLAERTRESGAAIAYLNVVGGQDALVFDGASVVADGDGTVHPAAAAFVDQW
LVVDYAAGERSFTPVVWVDDGDESMDALAWRAVVRGLQDYCRKNGFSKVWLGLSGGIDSALVLAMAVDALGGDNVTAVRL
PSRYTANLSNDLADEQCRALGVKLETIAIEPAFEGLLGALGPMFEGTQPDITEENLQSRSRGVILMALSNKFGGLVLTTG
NKSEYAVGYATIYGDMCGGYAPLKDLYKTEVFGLAKWRNTVGGAPVIPPAVISRPPSAELRDNQTDQDSLPPYDVLDGIL
YRYVDQEQSRDDIVAAGYAADTVEHVLRLVRLNEWKRHQSAPGPKVSRRAFGRERRYPITNGYRGQ

Sequences:

>Translated_546_residues
MSQTLRIAMAQFDFPVGGVAQNTDRIIEYIAAARDEFEADIVLFPELAISGYPPEDLLLRPGFLAHCEQALARIAAATRG
IVAVVGWPQSAGSVVYNAASVLRDGRIEATYRKRELPNYAVFDERRYFDVDPDGENCVVTVKGVRVGVVICEDLWFAEPL
ANTVRAGAELVLVPNASPYERGKHAQRDALLAERTRESGAAIAYLNVVGGQDALVFDGASVVADGDGTVHPAAAAFVDQW
LVVDYAAGERSFTPVVWVDDGDESMDALAWRAVVRGLQDYCRKNGFSKVWLGLSGGIDSALVLAMAVDALGGDNVTAVRL
PSRYTANLSNDLADEQCRALGVKLETIAIEPAFEGLLGALGPMFEGTQPDITEENLQSRSRGVILMALSNKFGGLVLTTG
NKSEYAVGYATIYGDMCGGYAPLKDLYKTEVFGLAKWRNTVGGAPVIPPAVISRPPSAELRDNQTDQDSLPPYDVLDGIL
YRYVDQEQSRDDIVAAGYAADTVEHVLRLVRLNEWKRHQSAPGPKVSRRAFGRERRYPITNGYRGQ
>Mature_545_residues
SQTLRIAMAQFDFPVGGVAQNTDRIIEYIAAARDEFEADIVLFPELAISGYPPEDLLLRPGFLAHCEQALARIAAATRGI
VAVVGWPQSAGSVVYNAASVLRDGRIEATYRKRELPNYAVFDERRYFDVDPDGENCVVTVKGVRVGVVICEDLWFAEPLA
NTVRAGAELVLVPNASPYERGKHAQRDALLAERTRESGAAIAYLNVVGGQDALVFDGASVVADGDGTVHPAAAAFVDQWL
VVDYAAGERSFTPVVWVDDGDESMDALAWRAVVRGLQDYCRKNGFSKVWLGLSGGIDSALVLAMAVDALGGDNVTAVRLP
SRYTANLSNDLADEQCRALGVKLETIAIEPAFEGLLGALGPMFEGTQPDITEENLQSRSRGVILMALSNKFGGLVLTTGN
KSEYAVGYATIYGDMCGGYAPLKDLYKTEVFGLAKWRNTVGGAPVIPPAVISRPPSAELRDNQTDQDSLPPYDVLDGILY
RYVDQEQSRDDIVAAGYAADTVEHVLRLVRLNEWKRHQSAPGPKVSRRAFGRERRYPITNGYRGQ

Specific function: Can use both glutamine or ammonia as a nitrogen source [H]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014445
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR000132
- InterPro:   IPR003010
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]

EC number: =6.3.5.1 [H]

Molecular weight: Translated: 59282; Mature: 59150

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQTLRIAMAQFDFPVGGVAQNTDRIIEYIAAARDEFEADIVLFPELAISGYPPEDLLLR
CCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCHHCCCCCCHHHEEC
PGFLAHCEQALARIAAATRGIVAVVGWPQSAGSVVYNAASVLRDGRIEATYRKRELPNYA
CCHHHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHCCCEEEHHHHHCCCCCE
VFDERRYFDVDPDGENCVVTVKGVRVGVVICEDLWFAEPLANTVRAGAELVLVPNASPYE
EECCCEEECCCCCCCCEEEEEECEEEEEEEECHHHHHHHHHHHHHCCCEEEEECCCCHHH
RGKHAQRDALLAERTRESGAAIAYLNVVGGQDALVFDGASVVADGDGTVHPAAAAFVDQW
HCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCEEEECCCEEEECCCCCCCHHHHHHHHHH
LVVDYAAGERSFTPVVWVDDGDESMDALAWRAVVRGLQDYCRKNGFSKVWLGLSGGIDSA
EEEEEECCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHH
LVLAMAVDALGGDNVTAVRLPSRYTANLSNDLADEQCRALGVKLETIAIEPAFEGLLGAL
HHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHCCEEEEEEECHHHHHHHHHH
GPMFEGTQPDITEENLQSRSRGVILMALSNKFGGLVLTTGNKSEYAVGYATIYGDMCGGY
CCCCCCCCCCCCHHHHHHCCCCEEEEEECCCCCCEEEEECCCCCEEEEEEEEEHHHCCCH
APLKDLYKTEVFGLAKWRNTVGGAPVIPPAVISRPPSAELRDNQTDQDSLPPYDVLDGIL
HHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHH
YRYVDQEQSRDDIVAAGYAADTVEHVLRLVRLNEWKRHQSAPGPKVSRRAFGRERRYPIT
HHHHCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCC
NGYRGQ
CCCCCC
>Mature Secondary Structure 
SQTLRIAMAQFDFPVGGVAQNTDRIIEYIAAARDEFEADIVLFPELAISGYPPEDLLLR
CCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCHHCCCCCCHHHEEC
PGFLAHCEQALARIAAATRGIVAVVGWPQSAGSVVYNAASVLRDGRIEATYRKRELPNYA
CCHHHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHCCCEEEHHHHHCCCCCE
VFDERRYFDVDPDGENCVVTVKGVRVGVVICEDLWFAEPLANTVRAGAELVLVPNASPYE
EECCCEEECCCCCCCCEEEEEECEEEEEEEECHHHHHHHHHHHHHCCCEEEEECCCCHHH
RGKHAQRDALLAERTRESGAAIAYLNVVGGQDALVFDGASVVADGDGTVHPAAAAFVDQW
HCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCEEEECCCEEEECCCCCCCHHHHHHHHHH
LVVDYAAGERSFTPVVWVDDGDESMDALAWRAVVRGLQDYCRKNGFSKVWLGLSGGIDSA
EEEEEECCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHH
LVLAMAVDALGGDNVTAVRLPSRYTANLSNDLADEQCRALGVKLETIAIEPAFEGLLGAL
HHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHCCEEEEEEECHHHHHHHHHH
GPMFEGTQPDITEENLQSRSRGVILMALSNKFGGLVLTTGNKSEYAVGYATIYGDMCGGY
CCCCCCCCCCCCHHHHHHCCCCEEEEEECCCCCCEEEEECCCCCEEEEEEEEEHHHCCCH
APLKDLYKTEVFGLAKWRNTVGGAPVIPPAVISRPPSAELRDNQTDQDSLPPYDVLDGIL
HHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHH
YRYVDQEQSRDDIVAAGYAADTVEHVLRLVRLNEWKRHQSAPGPKVSRRAFGRERRYPIT
HHHHCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCC
NGYRGQ
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]