Click here to switch to the map view.

The map label for this gene is btuB [H]

Identifier: 21243920

GI number: 21243920

Start: 3756234

End: 3758114

Strand: Reverse

Name: btuB [H]

Synonym: XAC3194

Alternate gene names: 21243920

Gene position: 3758114-3756234 (Counterclockwise)

Preceding gene: 21243921

Following gene: 77748706

Centisome position: 72.61

GC content: 65.5

Gene sequence:

>1881_bases
TTGATGTCCCTTTCTTCCGTTTCGCCGCGTCGTGCCGTGCTCGCCGTGGGGCTGTCGCTGTGCATTGCCACTGTGGCCCA
GGCCGAGGCCGCCATCGATCTGGACCAAGTCGTGGTCACCGCCTCGCGTACCGCACAGACCCAGGACCAGACGCTGGCGC
CGGTCACGGTGATCGACCGCGCGCAGATCGAGCGTCGTCAGGTCAATTCGCTGCAGGACCTGTTGCGTGGCGAAGCGGGC
GTGTCGCTGGCCAACAATGGCGGGCCTGGCAAGGCGACCTCGCTGTTCCTGCGCGGCACCGAGGCAGACCATGTGGTGGT
GCTGATCGATGGCGTGCGCATCGGCTCGGCCACCGCTGGCGGCGCGGCGTTGCAGGATCTGCCGATCGAGCAGATCGAAC
GCATCGAAATCGTGCGTGGCCCGTTTTCCAGCCTGTATGGCTCCGAAGCGCTGGGCGGGGTGGTCCAGATCTTTACCCGG
CGTCCGCAAGGCGGTTTGGTGCCGACCTTGAGCGTGGCGGCCGGCAGCGACAACGCGCGACGCTACGGCGCGGGCATTGC
GGGGCGCAGCCCGGGCGATCTGAGCGACGCCGGTGGTTGGTATTCGGTGAACGCCGTGCACGACGAAACCGACGGTATCA
ACGCGTATCTGGATACCGGCTCCAGCGCCTACGACCCGGACCGCGACGGCTATCGCAACGATTCGTTGAGCGCGCAGGGC
GGCTGGCGTTTCAATCGCCAGTGGGATGCCGATGTGCATGTATTGCGCGCGCAGAGCCGTAACGAATACGACGGTTCGGC
ATTCGGCGGCAATCTGAGCAAGGGCGTGCAGCAGGCCATCGGCGGCCGCGTGCGTTACGCGCCGAGCGATGCGCTCAAGC
TCACTGCCAGCGTGGGCAGCAGCGCCGATCTGAGCGATGCTTACTACCAAGGCGCCTATCTGTCGACCTACGACACCCGC
CGCAAGCAGGGTGCGTTGCAGGCCGATCTCGACACCGGCCCCGGTCTGCTCACGGTCGGCTTCGATTGGCAGCGCGACGC
CATCGCCAGCAGCGATACCTACGATGCCGACAGCCGCATCGACCGCGCCGCATTTGCGCAATGGCAGCAGACGTTTGGCA
GCCAATCGCTGCAGGCCAGCCTGCGTCGCAACGACAACAGCCAGTTCGGCGGCAAGACCACCGGCAGCCTGCTGTGGGGT
TGGGATGTCGCCGAGCATCTGCGCCTGACCGCCAGCTACGGCACTGCGTTCAAGGCGCCCACCTTCAATGAGTTGTATTA
CCCCGAATACGGAAACCCGCTGCTCGGCCCGGAAACCTCCAAGAGTGCCGAGCTCGGCCTGCGCGGTCGCTACGACTGGG
GCACCTGGACGCTCAGCGCGTTCCAGACCCGTATCGACGACCTCATCGCCTACGACGCCTCGTTGGTGGATGCGACGCAT
CCGTTCGGGCAGCCGAACAATATCGATCGGGCGCGCATTCGCGGCGTGGAAGCGGGCTACGACACCGAACTGGCCGGCTG
GACCGTGCGCAGCGCGTTGACCTGGCTTGCCCCGCAGGCCGATGGCGAGGTCAATCATGGCAACTGGTTGCCGCGGCGTG
CGCGTCAGAGTGGGCGCATCGATGCCGACCGCAACATCGGTGCATTCGGCATCGGCGCAAGCCTGTTCGGATCGGGTGCA
CGCTACGACGATCTGGCCAATACCGATCGCCTGGCCGGCTACGGCTTGCTGGATCTGCGCGTGAGTTATGCAGTGAACGC
GGACTGGAAGGTGCAACTGGCCGCCAACAATGTGTTCGACCGCCACTACGAAACCGCACGTTGGTACGCCCAGCCAGGCC
GCAATTATCTGCTGACCTTGCGCTACCAACCGGCGCACTGA

Upstream 100 bases:

>100_bases
GTGGCCTTGCTCGTCTGACGCTGCGCGCCGGACGCTTGGCCCGTTGCTGCCTGCCGCCCGATTGCCCACGCGCGGGTTGG
CGCGGTTCCAGGAGCAGCAG

Downstream 100 bases:

>100_bases
GAGCAGCCGACGACACGACTGCGCAGCCAACGGATGGGCGCGCGCGGTGCCCGGATCCGCATGGACCAGGCGGACACCGC
GGTTGCTCGCGCTGTCCGCG

Product: vitamin B transport outer membrane protein

Products: NA

Alternate protein names: Cobalamin receptor; Outer membrane cobalamin translocator [H]

Number of amino acids: Translated: 626; Mature: 626

Protein sequence:

>626_residues
MMSLSSVSPRRAVLAVGLSLCIATVAQAEAAIDLDQVVVTASRTAQTQDQTLAPVTVIDRAQIERRQVNSLQDLLRGEAG
VSLANNGGPGKATSLFLRGTEADHVVVLIDGVRIGSATAGGAALQDLPIEQIERIEIVRGPFSSLYGSEALGGVVQIFTR
RPQGGLVPTLSVAAGSDNARRYGAGIAGRSPGDLSDAGGWYSVNAVHDETDGINAYLDTGSSAYDPDRDGYRNDSLSAQG
GWRFNRQWDADVHVLRAQSRNEYDGSAFGGNLSKGVQQAIGGRVRYAPSDALKLTASVGSSADLSDAYYQGAYLSTYDTR
RKQGALQADLDTGPGLLTVGFDWQRDAIASSDTYDADSRIDRAAFAQWQQTFGSQSLQASLRRNDNSQFGGKTTGSLLWG
WDVAEHLRLTASYGTAFKAPTFNELYYPEYGNPLLGPETSKSAELGLRGRYDWGTWTLSAFQTRIDDLIAYDASLVDATH
PFGQPNNIDRARIRGVEAGYDTELAGWTVRSALTWLAPQADGEVNHGNWLPRRARQSGRIDADRNIGAFGIGASLFGSGA
RYDDLANTDRLAGYGLLDLRVSYAVNADWKVQLAANNVFDRHYETARWYAQPGRNYLLTLRYQPAH

Sequences:

>Translated_626_residues
MMSLSSVSPRRAVLAVGLSLCIATVAQAEAAIDLDQVVVTASRTAQTQDQTLAPVTVIDRAQIERRQVNSLQDLLRGEAG
VSLANNGGPGKATSLFLRGTEADHVVVLIDGVRIGSATAGGAALQDLPIEQIERIEIVRGPFSSLYGSEALGGVVQIFTR
RPQGGLVPTLSVAAGSDNARRYGAGIAGRSPGDLSDAGGWYSVNAVHDETDGINAYLDTGSSAYDPDRDGYRNDSLSAQG
GWRFNRQWDADVHVLRAQSRNEYDGSAFGGNLSKGVQQAIGGRVRYAPSDALKLTASVGSSADLSDAYYQGAYLSTYDTR
RKQGALQADLDTGPGLLTVGFDWQRDAIASSDTYDADSRIDRAAFAQWQQTFGSQSLQASLRRNDNSQFGGKTTGSLLWG
WDVAEHLRLTASYGTAFKAPTFNELYYPEYGNPLLGPETSKSAELGLRGRYDWGTWTLSAFQTRIDDLIAYDASLVDATH
PFGQPNNIDRARIRGVEAGYDTELAGWTVRSALTWLAPQADGEVNHGNWLPRRARQSGRIDADRNIGAFGIGASLFGSGA
RYDDLANTDRLAGYGLLDLRVSYAVNADWKVQLAANNVFDRHYETARWYAQPGRNYLLTLRYQPAH
>Mature_626_residues
MMSLSSVSPRRAVLAVGLSLCIATVAQAEAAIDLDQVVVTASRTAQTQDQTLAPVTVIDRAQIERRQVNSLQDLLRGEAG
VSLANNGGPGKATSLFLRGTEADHVVVLIDGVRIGSATAGGAALQDLPIEQIERIEIVRGPFSSLYGSEALGGVVQIFTR
RPQGGLVPTLSVAAGSDNARRYGAGIAGRSPGDLSDAGGWYSVNAVHDETDGINAYLDTGSSAYDPDRDGYRNDSLSAQG
GWRFNRQWDADVHVLRAQSRNEYDGSAFGGNLSKGVQQAIGGRVRYAPSDALKLTASVGSSADLSDAYYQGAYLSTYDTR
RKQGALQADLDTGPGLLTVGFDWQRDAIASSDTYDADSRIDRAAFAQWQQTFGSQSLQASLRRNDNSQFGGKTTGSLLWG
WDVAEHLRLTASYGTAFKAPTFNELYYPEYGNPLLGPETSKSAELGLRGRYDWGTWTLSAFQTRIDDLIAYDASLVDATH
PFGQPNNIDRARIRGVEAGYDTELAGWTVRSALTWLAPQADGEVNHGNWLPRRARQSGRIDADRNIGAFGIGASLFGSGA
RYDDLANTDRLAGYGLLDLRVSYAVNADWKVQLAANNVFDRHYETARWYAQPGRNYLLTLRYQPAH

Specific function: Involved in the active translocation of vitamin B12 (cyanocobalamin) across the outer membrane to the periplasmic space. It derives its energy for transport by interacting with the trans-periplasmic membrane protein TonB [H]

COG id: COG4206

COG function: function code H; Outer membrane cobalamin receptor protein

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TonB-dependent receptor family. BtuB (TC 1.B.14.3.1) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790405, Length=635, Percent_Identity=33.8582677165354, Blast_Score=235, Evalue=7e-63,
Organism=Escherichia coli, GI1788478, Length=673, Percent_Identity=25.8543833580981, Blast_Score=123, Evalue=3e-29,
Organism=Escherichia coli, GI1786798, Length=170, Percent_Identity=32.3529411764706, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1787723, Length=249, Percent_Identity=30.9236947791165, Blast_Score=63, Evalue=6e-11,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012910
- InterPro:   IPR000531 [H]

Pfam domain/function: PF07715 Plug; PF00593 TonB_dep_Rec [H]

EC number: NA

Molecular weight: Translated: 67630; Mature: 67630

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.3 %Met     (Translated Protein)
0.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
0.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMSLSSVSPRRAVLAVGLSLCIATVAQAEAAIDLDQVVVTASRTAQTQDQTLAPVTVIDR
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHEEEECCCCCCCCCCCCCCEEEHHH
AQIERRQVNSLQDLLRGEAGVSLANNGGPGKATSLFLRGTEADHVVVLIDGVRIGSATAG
HHHHHHHHHHHHHHHCCCCCCEECCCCCCCCEEEEEEECCCCCEEEEEEECEEECCCCCC
GAALQDLPIEQIERIEIVRGPFSSLYGSEALGGVVQIFTRRPQGGLVPTLSVAAGSDNAR
CCHHHCCCHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCH
RYGAGIAGRSPGDLSDAGGWYSVNAVHDETDGINAYLDTGSSAYDPDRDGYRNDSLSAQG
HHCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCC
GWRFNRQWDADVHVLRAQSRNEYDGSAFGGNLSKGVQQAIGGRVRYAPSDALKLTASVGS
CEEECCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHCCEEEECCCCCEEEEECCCC
SADLSDAYYQGAYLSTYDTRRKQGALQADLDTGPGLLTVGFDWQRDAIASSDTYDADSRI
CCCCCHHHHCCCEEEHHHHHHHCCCEEEECCCCCCEEEECCCCCCCCCCCCCCCCCHHHH
DRAAFAQWQQTFGSQSLQASLRRNDNSQFGGKTTGSLLWGWDVAEHLRLTASYGTAFKAP
HHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCEEECCCHHHHHEEEECCCCEECCC
TFNELYYPEYGNPLLGPETSKSAELGLRGRYDWGTWTLSAFQTRIDDLIAYDASLVDATH
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHCCHHHHCCC
PFGQPNNIDRARIRGVEAGYDTELAGWTVRSALTWLAPQADGEVNHGNWLPRRARQSGRI
CCCCCCCCCHHHHEECCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCC
DADRNIGAFGIGASLFGSGARYDDLANTDRLAGYGLLDLRVSYAVNADWKVQLAANNVFD
CCCCCCCEEECCHHHHCCCCCCCCCCCCCHHCCCEEEEEEEEEEECCCEEEEEECCCHHH
RHYETARWYAQPGRNYLLTLRYQPAH
HHHHHHHHCCCCCCCEEEEEEECCCC
>Mature Secondary Structure
MMSLSSVSPRRAVLAVGLSLCIATVAQAEAAIDLDQVVVTASRTAQTQDQTLAPVTVIDR
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHEEEECCCCCCCCCCCCCCEEEHHH
AQIERRQVNSLQDLLRGEAGVSLANNGGPGKATSLFLRGTEADHVVVLIDGVRIGSATAG
HHHHHHHHHHHHHHHCCCCCCEECCCCCCCCEEEEEEECCCCCEEEEEEECEEECCCCCC
GAALQDLPIEQIERIEIVRGPFSSLYGSEALGGVVQIFTRRPQGGLVPTLSVAAGSDNAR
CCHHHCCCHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCH
RYGAGIAGRSPGDLSDAGGWYSVNAVHDETDGINAYLDTGSSAYDPDRDGYRNDSLSAQG
HHCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCC
GWRFNRQWDADVHVLRAQSRNEYDGSAFGGNLSKGVQQAIGGRVRYAPSDALKLTASVGS
CEEECCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHCCEEEECCCCCEEEEECCCC
SADLSDAYYQGAYLSTYDTRRKQGALQADLDTGPGLLTVGFDWQRDAIASSDTYDADSRI
CCCCCHHHHCCCEEEHHHHHHHCCCEEEECCCCCCEEEECCCCCCCCCCCCCCCCCHHHH
DRAAFAQWQQTFGSQSLQASLRRNDNSQFGGKTTGSLLWGWDVAEHLRLTASYGTAFKAP
HHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCEEECCCHHHHHEEEECCCCEECCC
TFNELYYPEYGNPLLGPETSKSAELGLRGRYDWGTWTLSAFQTRIDDLIAYDASLVDATH
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHCCHHHHCCC
PFGQPNNIDRARIRGVEAGYDTELAGWTVRSALTWLAPQADGEVNHGNWLPRRARQSGRI
CCCCCCCCCHHHHEECCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCC
DADRNIGAFGIGASLFGSGARYDDLANTDRLAGYGLLDLRVSYAVNADWKVQLAANNVFD
CCCCCCCEEECCHHHHCCCCCCCCCCCCCHHCCCEEEEEEEEEEECCCEEEEEECCCHHH
RHYETARWYAQPGRNYLLTLRYQPAH
HHHHHHHHCCCCCCCEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA