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The map label for this gene is cobS [H]

Identifier: 21243910

GI number: 21243910

Start: 3747745

End: 3748479

Strand: Reverse

Name: cobS [H]

Synonym: XAC3184

Alternate gene names: 21243910

Gene position: 3748479-3747745 (Counterclockwise)

Preceding gene: 21243911

Following gene: 21243909

Centisome position: 72.43

GC content: 70.88

Gene sequence:

>735_bases
ATGATCGACGGGTTGCTGGCGGCGATCGGGTTTCTGACCCGTATTCCGGTGCCCGCCCGGGTGTTCACGCGCGCAGGCGC
GCAGGCGCGCTCGCTTTGCTGGTACCCGCTGGTGGGCGTGTTGCTCGGTGGGGTGGTGTGCGGGTTGGCCTGGTGCCTGC
GCGAGGTAGCGCCGTTGCTCGGCGCAGCGCTGCTGTTGAGCGCCTGGGTGTGGCTGACTGGCGCGTTGCATCTGGACGGC
CTGGCCGACACGACCGATGCCTGGGTTGGCGGCACGGGCGAGCGCGCACGCACACTGGCGATCATGAAGGACCCCACCAG
CGGCCCGATGGCAGTCACCGCAGTGGTGCTAGTGTTGCTGCTCAAATGCGCCGCATTGGCCAGCCTGCTGGCGCAGGCGC
CGACCACGCTATGGCTGGCGCCGCTACTGGCGCGCAGCGCGCTGGTTGCCGCGTTCCTGAGCACCTCGTATGTGCGCGCT
GTCGGGCTGGGCAGCGCGCTGTCGGGCGCCCCGCGGGCAGGCCTGTGGCTGGCGCTGAGCGCCGGCATCGCGGTGTGCAG
CGTTGCCGGGCTGGCGACCGCGGCGTTGTGCGTCGGCATCGCCGCACTGGTGTTCGCACTCTGGCGCCGCGCCTGCCTGC
AGCGCCTGGGCGGCATGACCGGCGATACCTGCGGCGCATTGGTAGAACTGACCGAAACGGCCGTGCTGGTGACGCTGGCG
CTGTGGTCGGCTTGA

Upstream 100 bases:

>100_bases
ATGCGTCCCTGCATCCGCTGGCCTGGCCGCCAACCGACGCGGCTGGCGCCTTTGGGTCGCCGGTTTCATCCACTGCATTG
CCACCGGCGCACGACACAGA

Downstream 100 bases:

>100_bases
CCAGGCAGGCGCTGCAGCTGACGCAATCGTCCTCCCGACTGATGACCCCAGCCCGCGCATCGTCTGTGCCTGCGTAGCGC
CCGTGGGCGCTGCGACCAAC

Product: cobalamin synthase

Products: GMP; adenosylcobalamin

Alternate protein names: NA

Number of amino acids: Translated: 244; Mature: 244

Protein sequence:

>244_residues
MIDGLLAAIGFLTRIPVPARVFTRAGAQARSLCWYPLVGVLLGGVVCGLAWCLREVAPLLGAALLLSAWVWLTGALHLDG
LADTTDAWVGGTGERARTLAIMKDPTSGPMAVTAVVLVLLLKCAALASLLAQAPTTLWLAPLLARSALVAAFLSTSYVRA
VGLGSALSGAPRAGLWLALSAGIAVCSVAGLATAALCVGIAALVFALWRRACLQRLGGMTGDTCGALVELTETAVLVTLA
LWSA

Sequences:

>Translated_244_residues
MIDGLLAAIGFLTRIPVPARVFTRAGAQARSLCWYPLVGVLLGGVVCGLAWCLREVAPLLGAALLLSAWVWLTGALHLDG
LADTTDAWVGGTGERARTLAIMKDPTSGPMAVTAVVLVLLLKCAALASLLAQAPTTLWLAPLLARSALVAAFLSTSYVRA
VGLGSALSGAPRAGLWLALSAGIAVCSVAGLATAALCVGIAALVFALWRRACLQRLGGMTGDTCGALVELTETAVLVTLA
LWSA
>Mature_244_residues
MIDGLLAAIGFLTRIPVPARVFTRAGAQARSLCWYPLVGVLLGGVVCGLAWCLREVAPLLGAALLLSAWVWLTGALHLDG
LADTTDAWVGGTGERARTLAIMKDPTSGPMAVTAVVLVLLLKCAALASLLAQAPTTLWLAPLLARSALVAAFLSTSYVRA
VGLGSALSGAPRAGLWLALSAGIAVCSVAGLATAALCVGIAALVFALWRRACLQRLGGMTGDTCGALVELTETAVLVTLA
LWSA

Specific function: Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin) [H]

COG id: COG0368

COG function: function code H; Cobalamin-5-phosphate synthase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobS family [H]

Homologues:

Organism=Escherichia coli, GI1788301, Length=251, Percent_Identity=35.4581673306773, Blast_Score=83, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003805 [H]

Pfam domain/function: PF02654 CobS [H]

EC number: 2.7.8.26

Molecular weight: Translated: 24871; Mature: 24871

Theoretical pI: Translated: 8.60; Mature: 8.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDGLLAAIGFLTRIPVPARVFTRAGAQARSLCWYPLVGVLLGGVVCGLAWCLREVAPLL
CCHHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GAALLLSAWVWLTGALHLDGLADTTDAWVGGTGERARTLAIMKDPTSGPMAVTAVVLVLL
HHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCHHHEEEEEECCCCCHHHHHHHHHHHH
LKCAALASLLAQAPTTLWLAPLLARSALVAAFLSTSYVRAVGLGSALSGAPRAGLWLALS
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCHHHHHHHH
AGIAVCSVAGLATAALCVGIAALVFALWRRACLQRLGGMTGDTCGALVELTETAVLVTLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
LWSA
HHCC
>Mature Secondary Structure
MIDGLLAAIGFLTRIPVPARVFTRAGAQARSLCWYPLVGVLLGGVVCGLAWCLREVAPLL
CCHHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GAALLLSAWVWLTGALHLDGLADTTDAWVGGTGERARTLAIMKDPTSGPMAVTAVVLVLL
HHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCHHHEEEEEECCCCCHHHHHHHHHHHH
LKCAALASLLAQAPTTLWLAPLLARSALVAAFLSTSYVRAVGLGSALSGAPRAGLWLALS
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCHHHHHHHH
AGIAVCSVAGLATAALCVGIAALVFALWRRACLQRLGGMTGDTCGALVELTETAVLVTLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
LWSA
HHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: adenosylcobinamide-GDP; alpha-ribazole

Specific reaction: adenosylcobinamide-GDP + alpha-ribazole = GMP + adenosylcobalamin

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA