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The map label for this gene is rsmE [H]

Identifier: 21243796

GI number: 21243796

Start: 3590077

End: 3590811

Strand: Direct

Name: rsmE [H]

Synonym: XAC3069

Alternate gene names: 21243796

Gene position: 3590077-3590811 (Clockwise)

Preceding gene: 21243795

Following gene: 21243798

Centisome position: 69.37

GC content: 68.3

Gene sequence:

>735_bases
ATGCGCCTGACCCGTTCGCATGTCGCGCTGCCGCTGCACTGCGACCAGGAAGTGACATTGCCGGAAGAGTCGGCCAATCA
CCTGCTGCGGGTACTGCGGCTGCGCGAGGGCGATGCCTGCATCCTGTTCAACGGCGACGGCAGCGACTACCACGCACGCA
TCACCGTGGCGGGCAAGCGCGATGCACGTGCGCGGGTGGAACGCGCCGAGCCGCTCTCCAACGAATCGCCGCTGCGTATC
ACCTTGATGCAAGGCATCGCGCGCGGCGAGAAGATGGATCTGATCCTGCAGAAGGCCACCGAATTGGGCGTTGCGGCGAT
CGTGCCGGTGATCGCCGAGCGCACCGAGGTCAAGCTCGATGCGGCGCGCACCGAAAAACGCGTGGCGCACTGGCGCAGCG
TGGTGGTGTCGGCCTGCGAGCAATCCGGGCGTGCACGTCTTCCCAGCGTGGCCGCGCCGCAGGGCCTGCAGGACGCGGCG
CAGGCCAGCGACGCGCATGCGCTACGGCTGACGCTGGACCCACAGGGCGAGCATCGCCTGGGCACGTTGGCATCGAGCAT
CCAGCAACACATCGTCGTGGCGATCGGGCCGGAAGGCGGCTGGTCGCCACGCGATCGCGCACTGCTTGCCGAGGCGGGTT
TCAGCGGACTGCAGCTAGGGCCGCGCATTCTGCGCACCGAAACGGCGGGGCTGGCGGCGATTGCGGCGCTACAGTCGCGG
TTCGGGGACCTATAG

Upstream 100 bases:

>100_bases
TTGGCGACGTGCTGTACTGGATGCCGCCGTATTGCGTGGACGAGGCGCAGCTTGCGTTGTTGGCCGACACCACCCGCCAT
GCCATCACGGAAGCGGTCGC

Downstream 100 bases:

>100_bases
CGCCATGACGCCGTAGGAGCGCACCCGGGCGCGACCAGGCGTTTATCGGCAACGCCGCGTCGCGCCCGGGTGCGCTCCTA
CCGGAATGCGGAGTTGATCG

Product: 16S ribosomal RNA methyltransferase RsmE

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase [H]

Number of amino acids: Translated: 244; Mature: 244

Protein sequence:

>244_residues
MRLTRSHVALPLHCDQEVTLPEESANHLLRVLRLREGDACILFNGDGSDYHARITVAGKRDARARVERAEPLSNESPLRI
TLMQGIARGEKMDLILQKATELGVAAIVPVIAERTEVKLDAARTEKRVAHWRSVVVSACEQSGRARLPSVAAPQGLQDAA
QASDAHALRLTLDPQGEHRLGTLASSIQQHIVVAIGPEGGWSPRDRALLAEAGFSGLQLGPRILRTETAGLAAIAALQSR
FGDL

Sequences:

>Translated_244_residues
MRLTRSHVALPLHCDQEVTLPEESANHLLRVLRLREGDACILFNGDGSDYHARITVAGKRDARARVERAEPLSNESPLRI
TLMQGIARGEKMDLILQKATELGVAAIVPVIAERTEVKLDAARTEKRVAHWRSVVVSACEQSGRARLPSVAAPQGLQDAA
QASDAHALRLTLDPQGEHRLGTLASSIQQHIVVAIGPEGGWSPRDRALLAEAGFSGLQLGPRILRTETAGLAAIAALQSR
FGDL
>Mature_244_residues
MRLTRSHVALPLHCDQEVTLPEESANHLLRVLRLREGDACILFNGDGSDYHARITVAGKRDARARVERAEPLSNESPLRI
TLMQGIARGEKMDLILQKATELGVAAIVPVIAERTEVKLDAARTEKRVAHWRSVVVSACEQSGRARLPSVAAPQGLQDAA
QASDAHALRLTLDPQGEHRLGTLASSIQQHIVVAIGPEGGWSPRDRALLAEAGFSGLQLGPRILRTETAGLAAIAALQSR
FGDL

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

Organism=Escherichia coli, GI87082186, Length=244, Percent_Identity=41.3934426229508, Blast_Score=184, Evalue=6e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015947
- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 26307; Mature: 26307

Theoretical pI: Translated: 8.14; Mature: 8.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLTRSHVALPLHCDQEVTLPEESANHLLRVLRLREGDACILFNGDGSDYHARITVAGKR
CCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEEEECCC
DARARVERAEPLSNESPLRITLMQGIARGEKMDLILQKATELGVAAIVPVIAERTEVKLD
CHHHHHHHCCCCCCCCCEEEEEHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHEEEH
AARTEKRVAHWRSVVVSACEQSGRARLPSVAAPQGLQDAAQASDAHALRLTLDPQGEHRL
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHCCCCEEEEEEECCCCCHHH
GTLASSIQQHIVVAIGPEGGWSPRDRALLAEAGFSGLQLGPRILRTETAGLAAIAALQSR
HHHHHHHHHEEEEEECCCCCCCCHHCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
FGDL
HCCC
>Mature Secondary Structure
MRLTRSHVALPLHCDQEVTLPEESANHLLRVLRLREGDACILFNGDGSDYHARITVAGKR
CCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEEEECCC
DARARVERAEPLSNESPLRITLMQGIARGEKMDLILQKATELGVAAIVPVIAERTEVKLD
CHHHHHHHCCCCCCCCCEEEEEHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHEEEH
AARTEKRVAHWRSVVVSACEQSGRARLPSVAAPQGLQDAAQASDAHALRLTLDPQGEHRL
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHCCCCEEEEEEECCCCCHHH
GTLASSIQQHIVVAIGPEGGWSPRDRALLAEAGFSGLQLGPRILRTETAGLAAIAALQSR
HHHHHHHHHEEEEEECCCCCCCCHHCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
FGDL
HCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8332061 [H]