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The map label for this gene is nifS [H]

Identifier: 21243665

GI number: 21243665

Start: 3442873

End: 3444117

Strand: Direct

Name: nifS [H]

Synonym: XAC2938

Alternate gene names: 21243665

Gene position: 3442873-3444117 (Clockwise)

Preceding gene: 21243664

Following gene: 21243666

Centisome position: 66.52

GC content: 65.22

Gene sequence:

>1245_bases
ATGAACGTGTCCGCCGTTCCGCCCAGCGCAACGCCCGACTGGGACCGCGTGCGCCTGGATTTCCCGCTGCTGATGCGGCA
GGTGCATGGCAAGCCGCTGATCTATTTCGACAACGCGAACACCGGCCAGAAGCCGCTGCAGGTGATTGCCGCCACCGACG
AGTTCTACCGCCGCCAGAACGCCAACGTAAGCCGTGCCGTGCATGCGCTCGGCAGCGAGGCGACCGACGCGTTCGAGGGA
GCGCGCAACAAACTGGCGCGTTTTCTCAACGTCCGCGCCGATGAGCTGGTGCTGTGCAGCGGCACCACCTTCGCGATCAA
TCTGGTGGCGTATTCGTGGGCATTGCCGCGGCTGGGCGCCGGCGATGTGATCCTGATTTCGCGCATGGAGCACCATGCCA
ACATCGTGCCGTGGCAGCTGGTGGCCCAGCGTACCGGCGCCACCATCCGCGTGGCCGAAATCACGCCCGATGGCGCGTTG
GATCTGGATGGCTTGCGCAAGGCGATGACACCTGACGTCAAACTGCTGGCGATCACCCACGTGTCCAACGTGCTGGGCAC
GGTCAACCCGGTACGCGAGATCTGCCGCGAAGCGCGTAAGCGCGGCATCGTCACCATGGTGGACGGCTCGCAGGCGGCAC
CGCATCGGCGCATCGACGTGGCCAGCATCGGCTGCGATTTCTATGCCATCACCGGGCACAAGATGTGCGGCCCCACCGGC
ACCGGCGCACTGTGGGCACGTCGCGAGCATCTGAAGGCGATGCCGCCGTTTCTTGGCGGCGGCGAGATGATCAAGGAAGT
CAGCTTCGAGAACACCGTCTTCAACGATGCGCCGCATAAGTTCGAAGCCGGCACGCCCAACATCGCCGGTTTCGTCGGCC
TGGGTGCGGCAGTGGATTACCTGGAATCGATCGGCCTGGCGCACGTGGAAGCACGCGAAGCCGAACTGCTCGCCCACTTC
ACCGAAGAACTGCAACGCATCGATGGCCTGCGCATCTTCGGCACCACGCCCGACAAGGCCGCGGTGGTGTCGTTCCTGAT
CGACGGTGCGCACGCGCACGACCTGGCCACCCTGCTCGACCTGGAAGGCGTCGCGGTGCGCTCGGGCCAACACTGCGCGC
ATCCGCTGCTGCAGTACTACGGTGTCGCCGCCACCTGCCGCGCGTCACTGGCGTTCTACAACACGCACGACGAAATCGAG
CGCTTCGTCGCCGCGTTGGTGAAGGTGCGTCGACTGTTGGGCTGA

Upstream 100 bases:

>100_bases
GTGCCGCGTTCTGTCACGAACCCTTGCGCGTGCTGCCGCCCGCCTTGAGCGCCGTGCTGGCGCTGCAACTGGACCGTGCA
TTGAATTCGGCGGGCGTGGC

Downstream 100 bases:

>100_bases
TGGGAATCGGGAATCGGGAATGGAGAATCGAAAGAGCGGTTCTCCCGAACGCCGGTGACATGCGATGTATGGAACACGGC
TCATCGAGCAGCGCAGCGCT

Product: cysteine desulfurase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 414; Mature: 414

Protein sequence:

>414_residues
MNVSAVPPSATPDWDRVRLDFPLLMRQVHGKPLIYFDNANTGQKPLQVIAATDEFYRRQNANVSRAVHALGSEATDAFEG
ARNKLARFLNVRADELVLCSGTTFAINLVAYSWALPRLGAGDVILISRMEHHANIVPWQLVAQRTGATIRVAEITPDGAL
DLDGLRKAMTPDVKLLAITHVSNVLGTVNPVREICREARKRGIVTMVDGSQAAPHRRIDVASIGCDFYAITGHKMCGPTG
TGALWARREHLKAMPPFLGGGEMIKEVSFENTVFNDAPHKFEAGTPNIAGFVGLGAAVDYLESIGLAHVEAREAELLAHF
TEELQRIDGLRIFGTTPDKAAVVSFLIDGAHAHDLATLLDLEGVAVRSGQHCAHPLLQYYGVAATCRASLAFYNTHDEIE
RFVAALVKVRRLLG

Sequences:

>Translated_414_residues
MNVSAVPPSATPDWDRVRLDFPLLMRQVHGKPLIYFDNANTGQKPLQVIAATDEFYRRQNANVSRAVHALGSEATDAFEG
ARNKLARFLNVRADELVLCSGTTFAINLVAYSWALPRLGAGDVILISRMEHHANIVPWQLVAQRTGATIRVAEITPDGAL
DLDGLRKAMTPDVKLLAITHVSNVLGTVNPVREICREARKRGIVTMVDGSQAAPHRRIDVASIGCDFYAITGHKMCGPTG
TGALWARREHLKAMPPFLGGGEMIKEVSFENTVFNDAPHKFEAGTPNIAGFVGLGAAVDYLESIGLAHVEAREAELLAHF
TEELQRIDGLRIFGTTPDKAAVVSFLIDGAHAHDLATLLDLEGVAVRSGQHCAHPLLQYYGVAATCRASLAFYNTHDEIE
RFVAALVKVRRLLG
>Mature_414_residues
MNVSAVPPSATPDWDRVRLDFPLLMRQVHGKPLIYFDNANTGQKPLQVIAATDEFYRRQNANVSRAVHALGSEATDAFEG
ARNKLARFLNVRADELVLCSGTTFAINLVAYSWALPRLGAGDVILISRMEHHANIVPWQLVAQRTGATIRVAEITPDGAL
DLDGLRKAMTPDVKLLAITHVSNVLGTVNPVREICREARKRGIVTMVDGSQAAPHRRIDVASIGCDFYAITGHKMCGPTG
TGALWARREHLKAMPPFLGGGEMIKEVSFENTVFNDAPHKFEAGTPNIAGFVGLGAAVDYLESIGLAHVEAREAELLAHF
TEELQRIDGLRIFGTTPDKAAVVSFLIDGAHAHDLATLLDLEGVAVRSGQHCAHPLLQYYGVAATCRASLAFYNTHDEIE
RFVAALVKVRRLLG

Specific function: Catalyzes the removal of elemental sulfur and selenium atoms from L-cysteine, L-cystine, L-selenocysteine, and L- selenocystine to produce L-alanine [H]

COG id: COG0520

COG function: function code E; Selenocysteine lyase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. Csd subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=394, Percent_Identity=22.5888324873096, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI156713448, Length=314, Percent_Identity=27.3885350318471, Blast_Score=84, Evalue=3e-16,
Organism=Escherichia coli, GI1787970, Length=402, Percent_Identity=48.2587064676617, Blast_Score=394, Evalue=1e-111,
Organism=Escherichia coli, GI1789175, Length=399, Percent_Identity=41.1027568922306, Blast_Score=287, Evalue=1e-78,
Organism=Escherichia coli, GI48994898, Length=410, Percent_Identity=25.1219512195122, Blast_Score=99, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI25143064, Length=349, Percent_Identity=23.7822349570201, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI193211090, Length=338, Percent_Identity=23.9644970414201, Blast_Score=79, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6319831, Length=230, Percent_Identity=29.5652173913043, Blast_Score=106, Evalue=7e-24,
Organism=Drosophila melanogaster, GI20129463, Length=259, Percent_Identity=28.1853281853282, Blast_Score=100, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR010970
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 45035; Mature: 45035

Theoretical pI: Translated: 7.10; Mature: 7.10

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVSAVPPSATPDWDRVRLDFPLLMRQVHGKPLIYFDNANTGQKPLQVIAATDEFYRRQN
CCCCCCCCCCCCCCCEEEECHHHHHHHHCCCEEEEECCCCCCCCCCEEEECCHHHHHHCC
ANVSRAVHALGSEATDAFEGARNKLARFLNVRADELVLCSGTTFAINLVAYSWALPRLGA
CCHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCEEEEEEHHHHHCCCCCCC
GDVILISRMEHHANIVPWQLVAQRTGATIRVAEITPDGALDLDGLRKAMTPDVKLLAITH
CCEEEEEEHHHCCCCCHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHCCCCEEEEEHHH
VSNVLGTVNPVREICREARKRGIVTMVDGSQAAPHRRIDVASIGCDFYAITGHKMCGPTG
HHHHHHCCHHHHHHHHHHHHCCEEEEECCCCCCCCCCEEEEEECCCEEEECCCCCCCCCC
TGALWARREHLKAMPPFLGGGEMIKEVSFENTVFNDAPHKFEAGTPNIAGFVGLGAAVDY
CCHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
LESIGLAHVEAREAELLAHFTEELQRIDGLRIFGTTPDKAAVVSFLIDGAHAHDLATLLD
HHHCCCHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHCCCCHHHHHHHHH
LEGVAVRSGQHCAHPLLQYYGVAATCRASLAFYNTHDEIERFVAALVKVRRLLG
CCCEEECCCCHHHHHHHHHHCCHHHHHHHHEEECCHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MNVSAVPPSATPDWDRVRLDFPLLMRQVHGKPLIYFDNANTGQKPLQVIAATDEFYRRQN
CCCCCCCCCCCCCCCEEEECHHHHHHHHCCCEEEEECCCCCCCCCCEEEECCHHHHHHCC
ANVSRAVHALGSEATDAFEGARNKLARFLNVRADELVLCSGTTFAINLVAYSWALPRLGA
CCHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCEEEEEEHHHHHCCCCCCC
GDVILISRMEHHANIVPWQLVAQRTGATIRVAEITPDGALDLDGLRKAMTPDVKLLAITH
CCEEEEEEHHHCCCCCHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHCCCCEEEEEHHH
VSNVLGTVNPVREICREARKRGIVTMVDGSQAAPHRRIDVASIGCDFYAITGHKMCGPTG
HHHHHHCCHHHHHHHHHHHHCCEEEEECCCCCCCCCCEEEEEECCCEEEECCCCCCCCCC
TGALWARREHLKAMPPFLGGGEMIKEVSFENTVFNDAPHKFEAGTPNIAGFVGLGAAVDY
CCHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
LESIGLAHVEAREAELLAHFTEELQRIDGLRIFGTTPDKAAVVSFLIDGAHAHDLATLLD
HHHCCCHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHCCCCHHHHHHHHH
LEGVAVRSGQHCAHPLLQYYGVAATCRASLAFYNTHDEIERFVAALVKVRRLLG
CCCEEECCCCHHHHHHHHHHCCHHHHHHHHEEECCHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]