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The map label for this gene is ogt [H]

Identifier: 21243550

GI number: 21243550

Start: 3308361

End: 3308906

Strand: Direct

Name: ogt [H]

Synonym: XAC2823

Alternate gene names: 21243550

Gene position: 3308361-3308906 (Clockwise)

Preceding gene: 21243549

Following gene: 21243551

Centisome position: 63.92

GC content: 67.58

Gene sequence:

>546_bases
ATGAGCACACTGCACTACGACACCTTCCCCTCGCCGATCGGCGCGCTCAGCGTGGCCGCCGACGACAAAGGCGTGCACCA
CATCCTGTTTGCGCAGAACCGCTACGACGCCATCGGCCGTGCGCGCTGGTTGCACGACCCCGATGCGCCGCTGGTGCGCG
AAGCACGCGAACAATTGCTCGATTACCTGCATGGCGGGCGACGCAGTTTCGATCTGCCGCTGGCGCCAGTCGGCACACCG
TTTCAACTGACGGTCTGGCGCACGCTGGCGCAGATTCCATTCGGGCAGACCTGGAGTTATGCGCAACTAGCGCAGGCGGT
CGGCAAACCGGCCGCCAGTCGCGCGGTGGGCGCCGCCAACGGACGCAACCCGCTGCCGATCGTGCTGCCCTGCCATCGCG
TGATCGGCGCCAATGGTGCGTTGACCGGCTTCGGCGGCGGCCTGCCGACCAAGCAGGCGTTGTTGCAGTTGGAAGGCTGG
TCGCCGCGTAGCGCCGCGCGCGCCGACGATCTGTTCGCACCTGCCTCGGCAATGCGCACCGGCTGA

Upstream 100 bases:

>100_bases
AACGCGCCACCGATGCCCGTTCGCAAGCGTGGCGACCGTGGCGCGCCTACGCCGTGCTGCACCTGTGGCATCTGGCCAAC
GACCGCAAGGACACCCGCGC

Downstream 100 bases:

>100_bases
CTTCGCGGCGTCATCGCTGCGCCGTAAACTTACGCCATGATCGACCGACGTTTTGGAGTTGCCGCAGCGGCGCTGGCCTT
GCTTGCGGCCTGCAGCAGCC

Product: 6-O-methylguanine-DNA methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 181; Mature: 180

Protein sequence:

>181_residues
MSTLHYDTFPSPIGALSVAADDKGVHHILFAQNRYDAIGRARWLHDPDAPLVREAREQLLDYLHGGRRSFDLPLAPVGTP
FQLTVWRTLAQIPFGQTWSYAQLAQAVGKPAASRAVGAANGRNPLPIVLPCHRVIGANGALTGFGGGLPTKQALLQLEGW
SPRSAARADDLFAPASAMRTG

Sequences:

>Translated_181_residues
MSTLHYDTFPSPIGALSVAADDKGVHHILFAQNRYDAIGRARWLHDPDAPLVREAREQLLDYLHGGRRSFDLPLAPVGTP
FQLTVWRTLAQIPFGQTWSYAQLAQAVGKPAASRAVGAANGRNPLPIVLPCHRVIGANGALTGFGGGLPTKQALLQLEGW
SPRSAARADDLFAPASAMRTG
>Mature_180_residues
STLHYDTFPSPIGALSVAADDKGVHHILFAQNRYDAIGRARWLHDPDAPLVREAREQLLDYLHGGRRSFDLPLAPVGTPF
QLTVWRTLAQIPFGQTWSYAQLAQAVGKPAASRAVGAANGRNPLPIVLPCHRVIGANGALTGFGGGLPTKQALLQLEGWS
PRSAARADDLFAPASAMRTG

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=171, Percent_Identity=33.3333333333333, Blast_Score=84, Evalue=8e-17,
Organism=Escherichia coli, GI1787596, Length=105, Percent_Identity=54.2857142857143, Blast_Score=115, Evalue=1e-27,
Organism=Escherichia coli, GI1788542, Length=161, Percent_Identity=33.5403726708075, Blast_Score=93, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI115533068, Length=85, Percent_Identity=44.7058823529412, Blast_Score=83, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI115533070, Length=85, Percent_Identity=44.7058823529412, Blast_Score=82, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6320001, Length=90, Percent_Identity=40, Blast_Score=81, Evalue=9e-17,
Organism=Drosophila melanogaster, GI17137554, Length=139, Percent_Identity=38.1294964028777, Blast_Score=77, Evalue=8e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 19397; Mature: 19266

Theoretical pI: Translated: 9.95; Mature: 9.95

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTLHYDTFPSPIGALSVAADDKGVHHILFAQNRYDAIGRARWLHDPDAPLVREAREQLL
CCCCCCCCCCCCCCEEEEECCCCCCEEEEEECCCHHHHHHHHCCCCCCCHHHHHHHHHHH
DYLHGGRRSFDLPLAPVGTPFQLTVWRTLAQIPFGQTWSYAQLAQAVGKPAASRAVGAAN
HHHHCCCCCCCCCCCCCCCCEEHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHCCCCC
GRNPLPIVLPCHRVIGANGALTGFGGGLPTKQALLQLEGWSPRSAARADDLFAPASAMRT
CCCCCEEEEECHHHHCCCCCCCCCCCCCCHHHHHEECCCCCCCCCCCHHHHHCCHHHHCC
G
C
>Mature Secondary Structure 
STLHYDTFPSPIGALSVAADDKGVHHILFAQNRYDAIGRARWLHDPDAPLVREAREQLL
CCCCCCCCCCCCCEEEEECCCCCCEEEEEECCCHHHHHHHHCCCCCCCHHHHHHHHHHH
DYLHGGRRSFDLPLAPVGTPFQLTVWRTLAQIPFGQTWSYAQLAQAVGKPAASRAVGAAN
HHHHCCCCCCCCCCCCCCCCEEHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHCCCCC
GRNPLPIVLPCHRVIGANGALTGFGGGLPTKQALLQLEGWSPRSAARADDLFAPASAMRT
CCCCCEEEEECHHHHCCCCCCCCCCCCCCHHHHHEECCCCCCCCCCCHHHHHCCHHHHCC
G
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]