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The map label for this gene is rluF [H]

Identifier: 21243538

GI number: 21243538

Start: 3296455

End: 3297177

Strand: Reverse

Name: rluF [H]

Synonym: XAC2811

Alternate gene names: 21243538

Gene position: 3297177-3296455 (Counterclockwise)

Preceding gene: 21243539

Following gene: 21243537

Centisome position: 63.71

GC content: 62.52

Gene sequence:

>723_bases
ATGACGATCCGACTCAACAAATACATCGCCGAAACCGGCTTTTGCTCACGCCGCGAGGCCGATCGTCTGATCGGCGAACG
CAGGGTCACCGTCAATGGCGTGCCCGCCGGTACCGGCGCGGTGGTCGGCGAGGAAGATACCGTGCTGGTGGATGACCAGC
CGCTACGCATACGCGAGGCCAAGAAGCTCGGTGGTCGCAAGCACGTCTACATCGCGCTCAACAAACCGGTGGGCATCACC
TGCACCACCGAGAGTTCGGTCAAGGGAAATATCGTCGAATTCGTCGGTCACGAGCAGCGCATCTTCCCGATCGGCCGGCT
CGACAAGGACTCCGAAGGGCTGATCCTGATGACCAGCAACGGCAACATCGTCAACGAGATCCTGCGCGCGGAAAATCGTC
ACCAGAAGGAATACCTGGTCGCGGTCAACAAGCCGGTCACCGACGAATTCTTGCGCGGCATGGCGCGCGGCGTGCGCATC
CACAACGAAACCACGCTGCCGTGCCGCACCGCGCGCATTGCCAAGTTCGGCTTCCGCATCGTGCTGGAGCAGGGCCTGAA
TCGACAGATTCGCCTGATGGCTGCCGAGTTCGGCTACCGGGTGACGCAACTGCGCCGGGTGCGCATCGACAACATCAAGC
TGGCTGCGCTCAAGCCGGGGCAGTGGCGCAACCTCAGCGATGCGGAATTGCGCGGGCTGCTGCCGCAACGCACCGACTGG
TAA

Upstream 100 bases:

>100_bases
CAGGCGACTTCCCGCAGCGTCATGCCGGTGCTGCGTGTACACGCCTCTGGCGGCTGCACGGTAGTTTTGTCGGCTGCTCT
AAATCGCCGATAATTGGCAG

Downstream 100 bases:

>100_bases
GCGCCTGTGCCGGGCCGACTGCATCGCTGTTAGGCCAGCGCAGCCGGCCTTTCGGACACGGCACATGCCCGCGCTGCAGT
GCCGTGCTGCCGCGCCCAAC

Product: pseudouridylate synthase

Products: NA

Alternate protein names: rRNA pseudouridylate synthase F; rRNA-uridine isomerase F [H]

Number of amino acids: Translated: 240; Mature: 239

Protein sequence:

>240_residues
MTIRLNKYIAETGFCSRREADRLIGERRVTVNGVPAGTGAVVGEEDTVLVDDQPLRIREAKKLGGRKHVYIALNKPVGIT
CTTESSVKGNIVEFVGHEQRIFPIGRLDKDSEGLILMTSNGNIVNEILRAENRHQKEYLVAVNKPVTDEFLRGMARGVRI
HNETTLPCRTARIAKFGFRIVLEQGLNRQIRLMAAEFGYRVTQLRRVRIDNIKLAALKPGQWRNLSDAELRGLLPQRTDW

Sequences:

>Translated_240_residues
MTIRLNKYIAETGFCSRREADRLIGERRVTVNGVPAGTGAVVGEEDTVLVDDQPLRIREAKKLGGRKHVYIALNKPVGIT
CTTESSVKGNIVEFVGHEQRIFPIGRLDKDSEGLILMTSNGNIVNEILRAENRHQKEYLVAVNKPVTDEFLRGMARGVRI
HNETTLPCRTARIAKFGFRIVLEQGLNRQIRLMAAEFGYRVTQLRRVRIDNIKLAALKPGQWRNLSDAELRGLLPQRTDW
>Mature_239_residues
TIRLNKYIAETGFCSRREADRLIGERRVTVNGVPAGTGAVVGEEDTVLVDDQPLRIREAKKLGGRKHVYIALNKPVGITC
TTESSVKGNIVEFVGHEQRIFPIGRLDKDSEGLILMTSNGNIVNEILRAENRHQKEYLVAVNKPVTDEFLRGMARGVRIH
NETTLPCRTARIAKFGFRIVLEQGLNRQIRLMAAEFGYRVTQLRRVRIDNIKLAALKPGQWRNLSDAELRGLLPQRTDW

Specific function: Responsible for synthesis of pseudouridine from uracil- 2604 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790453, Length=233, Percent_Identity=54.0772532188841, Blast_Score=234, Evalue=3e-63,
Organism=Escherichia coli, GI1788510, Length=239, Percent_Identity=34.3096234309623, Blast_Score=114, Evalue=5e-27,
Organism=Escherichia coli, GI1787524, Length=245, Percent_Identity=28.9795918367347, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI87081838, Length=177, Percent_Identity=28.8135593220339, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 27125; Mature: 26994

Theoretical pI: Translated: 10.60; Mature: 10.60

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIRLNKYIAETGFCSRREADRLIGERRVTVNGVPAGTGAVVGEEDTVLVDDQPLRIREA
CEEEECHHHHHCCCCCHHHHHHHHCCCEEEEECCCCCCCCEECCCCEEEECCCCCHHHHH
KKLGGRKHVYIALNKPVGITCTTESSVKGNIVEFVGHEQRIFPIGRLDKDSEGLILMTSN
HHCCCCEEEEEEECCCEEEEEECCCCCCCCCEEECCCCCEEEECCCCCCCCCCEEEEECC
GNIVNEILRAENRHQKEYLVAVNKPVTDEFLRGMARGVRIHNETTLPCRTARIAKFGFRI
CHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHCEEEECCCCCCCHHHHHHHHHHHH
VLEQGLNRQIRLMAAEFGYRVTQLRRVRIDNIKLAALKPGQWRNLSDAELRGLLPQRTDW
EEHHCCCCEEEEEEHHHCHHHHHHEEEEECCEEEEEECCCCCCCCCHHHHHCCCCCCCCC
>Mature Secondary Structure 
TIRLNKYIAETGFCSRREADRLIGERRVTVNGVPAGTGAVVGEEDTVLVDDQPLRIREA
EEEECHHHHHCCCCCHHHHHHHHCCCEEEEECCCCCCCCEECCCCEEEECCCCCHHHHH
KKLGGRKHVYIALNKPVGITCTTESSVKGNIVEFVGHEQRIFPIGRLDKDSEGLILMTSN
HHCCCCEEEEEEECCCEEEEEECCCCCCCCCEEECCCCCEEEECCCCCCCCCCEEEEECC
GNIVNEILRAENRHQKEYLVAVNKPVTDEFLRGMARGVRIHNETTLPCRTARIAKFGFRI
CHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHCEEEECCCCCCCHHHHHHHHHHHH
VLEQGLNRQIRLMAAEFGYRVTQLRRVRIDNIKLAALKPGQWRNLSDAELRGLLPQRTDW
EEHHCCCCEEEEEEHHHCHHHHHHEEEEECCEEEEEECCCCCCCCCHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]