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The map label for this gene is ysgA [C]

Identifier: 21243463

GI number: 21243463

Start: 3200304

End: 3200966

Strand: Direct

Name: ysgA [C]

Synonym: XAC2736

Alternate gene names: 21243463

Gene position: 3200304-3200966 (Clockwise)

Preceding gene: 21243461

Following gene: 21243464

Centisome position: 61.84

GC content: 66.52

Gene sequence:

>663_bases
ATGGGTCACTGGACCACGCTCGACACCCCCGACGGCCAGGTTGCCGCCTGGCACGCCACGCCCGCTTCCAGCCCGCGCGG
CGGCCTGGTGGTGATTCAAGAAATCTTCGGTGTCAACGAACATATCCGCGCAGTGGCCGACGACTACGCCGCACGCGGCT
ATGAGGTGCTGGCACCGGCCTTTTTCGATCTGGAAGAGAAGGACGTGCAGTTGCCGTACGACCAGGAAAGCCTGCAGCGG
GGCCTGGCGCTGGCCAATGCGGTGGGCCTGGAGCGCGCGGTGGAGGTGGTGAAATCTGCCGCCACCCTGCTGACCCGCGC
CGGCAAGGTGGGCACGGTGGGCTACTGCTGGGGTGGCTCGGTCGCACTGCTGTCGGCGATACGCCTGGGACTGCCGTCGG
TGAGCTACTACGGCGGCCGCAACACCCAGCTGCTGGACGAAACCCCCAAGGCACCGGTGATGTTCCACTTCGGCGAGCGC
GATGCCAGCATTCCGCCCGAAGCGATCCAGGCCCACCGCGAGAAACTGCCGCAGATGGAAACCTTCGTGTATCCGACCGG
ACATGCCTTCAACCGCAGCATCGACCCCACCCACTACGACGCCGACAGCGCCGAGCGCGCATTGGAGCGCACCCTGGGCT
TCTTCGCGGCGCATCTGGGATGA

Upstream 100 bases:

>100_bases
GCATGCCGCGCACGCGGGGGCATCTTAGCGGAACCCTCACCTTCGCCCCCGCCGATCCGGTCGATAATGCGCGCCTCCCC
GGACTGCAGCAGGAGTTTCC

Downstream 100 bases:

>100_bases
GCGACACCGCACCGGGCACGCCGGCGGGCGGTTTCCTGCTCGATCCGCGCCTGGCGGCCGACACGGTGTTCGTCGCCGAC
GGGCCGCTGTCGCAGGTGCG

Product: carboxymethylenebutenolidase

Products: NA

Alternate protein names: Dienelactone hydrolase; DLH [H]

Number of amino acids: Translated: 220; Mature: 219

Protein sequence:

>220_residues
MGHWTTLDTPDGQVAAWHATPASSPRGGLVVIQEIFGVNEHIRAVADDYAARGYEVLAPAFFDLEEKDVQLPYDQESLQR
GLALANAVGLERAVEVVKSAATLLTRAGKVGTVGYCWGGSVALLSAIRLGLPSVSYYGGRNTQLLDETPKAPVMFHFGER
DASIPPEAIQAHREKLPQMETFVYPTGHAFNRSIDPTHYDADSAERALERTLGFFAAHLG

Sequences:

>Translated_220_residues
MGHWTTLDTPDGQVAAWHATPASSPRGGLVVIQEIFGVNEHIRAVADDYAARGYEVLAPAFFDLEEKDVQLPYDQESLQR
GLALANAVGLERAVEVVKSAATLLTRAGKVGTVGYCWGGSVALLSAIRLGLPSVSYYGGRNTQLLDETPKAPVMFHFGER
DASIPPEAIQAHREKLPQMETFVYPTGHAFNRSIDPTHYDADSAERALERTLGFFAAHLG
>Mature_219_residues
GHWTTLDTPDGQVAAWHATPASSPRGGLVVIQEIFGVNEHIRAVADDYAARGYEVLAPAFFDLEEKDVQLPYDQESLQRG
LALANAVGLERAVEVVKSAATLLTRAGKVGTVGYCWGGSVALLSAIRLGLPSVSYYGGRNTQLLDETPKAPVMFHFGERD
ASIPPEAIQAHREKLPQMETFVYPTGHAFNRSIDPTHYDADSAERALERTLGFFAAHLG

Specific function: Unknown

COG id: COG0412

COG function: function code Q; Dienelactone hydrolase and related enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dienelactone hydrolase family [H]

Homologues:

Organism=Escherichia coli, GI48994982, Length=240, Percent_Identity=30.8333333333333, Blast_Score=89, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002925 [H]

Pfam domain/function: PF01738 DLH [H]

EC number: =3.1.1.45 [H]

Molecular weight: Translated: 23862; Mature: 23731

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGHWTTLDTPDGQVAAWHATPASSPRGGLVVIQEIFGVNEHIRAVADDYAARGYEVLAPA
CCCCCCCCCCCCCEEEEECCCCCCCCCCEEEHHHHHCCCHHHHHHHHHHHHCCCHHHHHH
FFDLEEKDVQLPYDQESLQRGLALANAVGLERAVEVVKSAATLLTRAGKVGTVGYCWGGS
HHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCH
VALLSAIRLGLPSVSYYGGRNTQLLDETPKAPVMFHFGERDASIPPEAIQAHREKLPQME
HHHHHHHHHCCCCCEECCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCHH
TFVYPTGHAFNRSIDPTHYDADSAERALERTLGFFAAHLG
EEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
GHWTTLDTPDGQVAAWHATPASSPRGGLVVIQEIFGVNEHIRAVADDYAARGYEVLAPA
CCCCCCCCCCCCEEEEECCCCCCCCCCEEEHHHHHCCCHHHHHHHHHHHHCCCHHHHHH
FFDLEEKDVQLPYDQESLQRGLALANAVGLERAVEVVKSAATLLTRAGKVGTVGYCWGGS
HHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCH
VALLSAIRLGLPSVSYYGGRNTQLLDETPKAPVMFHFGERDASIPPEAIQAHREKLPQME
HHHHHHHHHCCCCCEECCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCHH
TFVYPTGHAFNRSIDPTHYDADSAERALERTLGFFAAHLG
EEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8138139 [H]