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The map label for this gene is greB

Identifier: 21243461

GI number: 21243461

Start: 3197922

End: 3198428

Strand: Direct

Name: greB

Synonym: XAC2734

Alternate gene names: 21243461

Gene position: 3197922-3198428 (Clockwise)

Preceding gene: 77748670

Following gene: 21243463

Centisome position: 61.79

GC content: 65.68

Gene sequence:

>507_bases
ATGAGCCGCTGGCGCCCACCTGCCGAAAAAAGCACTGCGCTGATCACGCCCGAAGGCCACGCCCGCCTCAAGGCCGAGCT
GGACGACTTGTGGCGCGTGCGCCGCCCCGAGGTGGTACGGGCCCTGGCGGCAGCGGCCGCCGAAGGCGATCGCTCGGAAA
ATGCCGAATACACCTATCGCAAGAAGCAGCTCGGTGAAATCGATCGGCGTGTGCGCTATCTGAGCAAGCGACTGGAAGCG
TTGCGCGTGGTGGATACCGCACCTACCGACGCGAACGCAGTGTTCTTCGGCGCACAGGTGGAGCTGGAAGACGCCGGCAG
TGGCGAGCTGCTGCGCTACCGCATCGTCGGCCCGGACGAAACCGATGCCGGACGCGGCTGGATCAGCATCGACTCCCCCC
TGGCGCGCGCCCTGCTGAAAAAGCGCGTGGACGACGAATTCGACGCACACTTGCCGGCCGGCAAGCACACATTCGTCGTG
GTGTCGGTGGATTACGCCTCGCTGTAG

Upstream 100 bases:

>100_bases
GTGTCCGAGAATGGCGCAAAGCTTCGCTGTGCGGCGGAAGATGCGCAAGCACGTCATGCGCGCTAGCATCGCGGGCCCGC
CGTTCTAACCTCCCTGCCCC

Downstream 100 bases:

>100_bases
GGGATACCCAAGGGGCGGATCGATCCTGGCGCCGTGCATCAGCAACGACAGTGATCGACACCCGGAGAACCGAACACCCA
GTGCCCCAGGACTGCATCGT

Product: transcription elongation factor GreB

Products: NA

Alternate protein names: Transcript cleavage factor greB

Number of amino acids: Translated: 168; Mature: 167

Protein sequence:

>168_residues
MSRWRPPAEKSTALITPEGHARLKAELDDLWRVRRPEVVRALAAAAAEGDRSENAEYTYRKKQLGEIDRRVRYLSKRLEA
LRVVDTAPTDANAVFFGAQVELEDAGSGELLRYRIVGPDETDAGRGWISIDSPLARALLKKRVDDEFDAHLPAGKHTFVV
VSVDYASL

Sequences:

>Translated_168_residues
MSRWRPPAEKSTALITPEGHARLKAELDDLWRVRRPEVVRALAAAAAEGDRSENAEYTYRKKQLGEIDRRVRYLSKRLEA
LRVVDTAPTDANAVFFGAQVELEDAGSGELLRYRIVGPDETDAGRGWISIDSPLARALLKKRVDDEFDAHLPAGKHTFVV
VSVDYASL
>Mature_167_residues
SRWRPPAEKSTALITPEGHARLKAELDDLWRVRRPEVVRALAAAAAEGDRSENAEYTYRKKQLGEIDRRVRYLSKRLEAL
RVVDTAPTDANAVFFGAQVELEDAGSGELLRYRIVGPDETDAGRGWISIDSPLARALLKKRVDDEFDAHLPAGKHTFVVV
SVDYASL

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family

Homologues:

Organism=Escherichia coli, GI87082261, Length=155, Percent_Identity=52.9032258064516, Blast_Score=154, Evalue=2e-39,
Organism=Escherichia coli, GI87082225, Length=152, Percent_Identity=39.4736842105263, Blast_Score=103, Evalue=5e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GREB_XANAC (Q8PJ11)

Other databases:

- EMBL:   AE008923
- RefSeq:   NP_643043.1
- ProteinModelPortal:   Q8PJ11
- SMR:   Q8PJ11
- GeneID:   1156805
- GenomeReviews:   AE008923_GR
- KEGG:   xac:XAC2734
- NMPDR:   fig|190486.1.peg.2687
- HOGENOM:   HBG753387
- OMA:   KRFRIVG
- ProtClustDB:   PRK01885
- BioCyc:   XAXO190486:XAC2734-MONOMER
- GO:   GO:0006350
- HAMAP:   MF_00105
- InterPro:   IPR018151
- InterPro:   IPR001437
- InterPro:   IPR022691
- InterPro:   IPR006358
- Gene3D:   G3DSA:1.10.287.180
- Gene3D:   G3DSA:3.10.50.30
- TIGRFAMs:   TIGR01461

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N; SSF46557 GreA_GreB

EC number: NA

Molecular weight: Translated: 18771; Mature: 18640

Theoretical pI: Translated: 6.82; Mature: 6.82

Prosite motif: PS00829 GREAB_1; PS00830 GREAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
0.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRWRPPAEKSTALITPEGHARLKAELDDLWRVRRPEVVRALAAAAAEGDRSENAEYTYR
CCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCHHHH
KKQLGEIDRRVRYLSKRLEALRVVDTAPTDANAVFFGAQVELEDAGSGELLRYRIVGPDE
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEECCCCCCCEEEEEEECCCC
TDAGRGWISIDSPLARALLKKRVDDEFDAHLPAGKHTFVVVSVDYASL
CCCCCEEEEECCHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEECCCCC
>Mature Secondary Structure 
SRWRPPAEKSTALITPEGHARLKAELDDLWRVRRPEVVRALAAAAAEGDRSENAEYTYR
CCCCCCCCCCEEEECCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCHHHH
KKQLGEIDRRVRYLSKRLEALRVVDTAPTDANAVFFGAQVELEDAGSGELLRYRIVGPDE
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEECCCCCCCEEEEEEECCCC
TDAGRGWISIDSPLARALLKKRVDDEFDAHLPAGKHTFVVVSVDYASL
CCCCCEEEEECCHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217