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The map label for this gene is truA

Identifier: 21243447

GI number: 21243447

Start: 3180487

End: 3181260

Strand: Reverse

Name: truA

Synonym: XAC2720

Alternate gene names: 21243447

Gene position: 3181260-3180487 (Counterclockwise)

Preceding gene: 21243448

Following gene: 21243446

Centisome position: 61.47

GC content: 66.15

Gene sequence:

>774_bases
ATGCGTTACGCGCTGGGCGTGGAATACGACGGCAGCGAGTTCCAGGGCTGGCAGCAGTTGGGCGAGCATGGCGGGCCGAG
CGTGCAGGCCAGCCTGCAGGCAGCTTTGTCGTCGGTGGCCGATGCCCCTGTGCAGGTGGTGTGCGCAGGCCGCACCGATG
CCGGCGTGCACGGCGAATGCCAGGTGGTGCATTTCGACAGCGATGCGCGCCGCGAGCCGCGTGGCTGGATGCTGGGCACC
ACTGCGCGCCTGCCGCCGTCGATCGCCGTGCGCTGGTGCGTACCGGCTGCCGATGACTTCCACGCGCGCTTTTCCGCGCG
CGCGCGTCGTTACCGCTATCGCCTGCTCAATCGGCAGATTCGCCCGGCCCTGTATCGGCAGACCCTGAGCTGGGAGCGTC
GCCCGCTCGACGCCGATGCCATGCATGGCGCAGCGCAGGCGTTATTGGGCGAGAACGATTTCAGCGCGTTCCGCAGCGTG
CAATGTCAGGCCTTGCACGCGCGCCGTCACCTGCAGGCCATCCATGTGCAGCGTATCGGCGAGGTGGTGGAGGTGCAGGT
CCAGGCCAATGCATTCCTTCATCACATGGTGCGGAATATTGTGGGTTCGCTGATTTTGGTCGGTACCGGCGAACAGCCGG
CCGACTGGATCGCGACGTTGCTCGCCGGACGCGACCGCACTGTGGCCGGCCCGACAGCGCCGCCACAAGGTCTGGTATTT
ATCGGACCTCTGTATCCTGCAGAGTGGCATCTGCCTGCTGAGGTGACCCAATGA

Upstream 100 bases:

>100_bases
CACCTTCTTTGCAGCGGCGGGGCTGTGCTGGCAATCGCCGGCGATGGCGGCAGCATGACCGGACGCAGATTTCATCGATT
CACTCATAGGGCAGGTACCG

Downstream 100 bases:

>100_bases
ATCGATCGCTGTACCGCACCCGTATCAAGTTTTGTGGCATGACCCGCGCCGGCGATATCCGGCTGGCCGGCGAACTGGGT
GTGGACGCGGTAGGTTTCAT

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MRYALGVEYDGSEFQGWQQLGEHGGPSVQASLQAALSSVADAPVQVVCAGRTDAGVHGECQVVHFDSDARREPRGWMLGT
TARLPPSIAVRWCVPAADDFHARFSARARRYRYRLLNRQIRPALYRQTLSWERRPLDADAMHGAAQALLGENDFSAFRSV
QCQALHARRHLQAIHVQRIGEVVEVQVQANAFLHHMVRNIVGSLILVGTGEQPADWIATLLAGRDRTVAGPTAPPQGLVF
IGPLYPAEWHLPAEVTQ

Sequences:

>Translated_257_residues
MRYALGVEYDGSEFQGWQQLGEHGGPSVQASLQAALSSVADAPVQVVCAGRTDAGVHGECQVVHFDSDARREPRGWMLGT
TARLPPSIAVRWCVPAADDFHARFSARARRYRYRLLNRQIRPALYRQTLSWERRPLDADAMHGAAQALLGENDFSAFRSV
QCQALHARRHLQAIHVQRIGEVVEVQVQANAFLHHMVRNIVGSLILVGTGEQPADWIATLLAGRDRTVAGPTAPPQGLVF
IGPLYPAEWHLPAEVTQ
>Mature_257_residues
MRYALGVEYDGSEFQGWQQLGEHGGPSVQASLQAALSSVADAPVQVVCAGRTDAGVHGECQVVHFDSDARREPRGWMLGT
TARLPPSIAVRWCVPAADDFHARFSARARRYRYRLLNRQIRPALYRQTLSWERRPLDADAMHGAAQALLGENDFSAFRSV
QCQALHARRHLQAIHVQRIGEVVEVQVQANAFLHHMVRNIVGSLILVGTGEQPADWIATLLAGRDRTVAGPTAPPQGLVF
IGPLYPAEWHLPAEVTQ

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family

Homologues:

Organism=Homo sapiens, GI23503259, Length=275, Percent_Identity=30.9090909090909, Blast_Score=84, Evalue=2e-16,
Organism=Escherichia coli, GI1788657, Length=251, Percent_Identity=54.5816733067729, Blast_Score=271, Evalue=4e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUA_XANAC (Q8PJ25)

Other databases:

- EMBL:   AE008923
- RefSeq:   NP_643029.1
- ProteinModelPortal:   Q8PJ25
- SMR:   Q8PJ25
- GeneID:   1156791
- GenomeReviews:   AE008923_GR
- KEGG:   xac:XAC2720
- NMPDR:   fig|190486.1.peg.2673
- HOGENOM:   HBG621020
- OMA:   QSKTPFR
- ProtClustDB:   PRK00021
- BioCyc:   XAXO190486:XAC2720-MONOMER
- BRENDA:   5.4.99.12
- HAMAP:   MF_00171
- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094
- Gene3D:   G3DSA:3.30.70.580
- Gene3D:   G3DSA:3.30.70.660
- PANTHER:   PTHR11142
- PIRSF:   PIRSF001430
- TIGRFAMs:   TIGR00071

Pfam domain/function: PF01416 PseudoU_synth_1; SSF55120 PsdUridine_synth_cat_dom

EC number: =5.4.99.12

Molecular weight: Translated: 28349; Mature: 28349

Theoretical pI: Translated: 8.04; Mature: 8.04

Prosite motif: NA

Important sites: ACT_SITE 53-53

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRYALGVEYDGSEFQGWQQLGEHGGPSVQASLQAALSSVADAPVQVVCAGRTDAGVHGEC
CCEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCEE
QVVHFDSDARREPRGWMLGTTARLPPSIAVRWCVPAADDFHARFSARARRYRYRLLNRQI
EEEEECCCCCCCCCCEEEECCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHH
RPALYRQTLSWERRPLDADAMHGAAQALLGENDFSAFRSVQCQALHARRHLQAIHVQRIG
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
EVVEVQVQANAFLHHMVRNIVGSLILVGTGEQPADWIATLLAGRDRTVAGPTAPPQGLVF
HHEEEEEHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCEEE
IGPLYPAEWHLPAEVTQ
EECCCCCCCCCCCCCCC
>Mature Secondary Structure
MRYALGVEYDGSEFQGWQQLGEHGGPSVQASLQAALSSVADAPVQVVCAGRTDAGVHGEC
CCEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCEE
QVVHFDSDARREPRGWMLGTTARLPPSIAVRWCVPAADDFHARFSARARRYRYRLLNRQI
EEEEECCCCCCCCCCEEEECCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHH
RPALYRQTLSWERRPLDADAMHGAAQALLGENDFSAFRSVQCQALHARRHLQAIHVQRIG
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
EVVEVQVQANAFLHHMVRNIVGSLILVGTGEQPADWIATLLAGRDRTVAGPTAPPQGLVF
HHEEEEEHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCEEE
IGPLYPAEWHLPAEVTQ
EECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217