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The map label for this gene is trpF

Identifier: 21243446

GI number: 21243446

Start: 3179822

End: 3180490

Strand: Reverse

Name: trpF

Synonym: XAC2719

Alternate gene names: 21243446

Gene position: 3180490-3179822 (Counterclockwise)

Preceding gene: 21243447

Following gene: 21243444

Centisome position: 61.45

GC content: 63.83

Gene sequence:

>669_bases
ATGAATCGATCGCTGTACCGCACCCGTATCAAGTTTTGTGGCATGACCCGCGCCGGCGATATCCGGCTGGCCGGCGAACT
GGGTGTGGACGCGGTAGGTTTCATCTTTGCGCATGGCAGTCCGCGGCGCGTGGCGCCGGCCGAGGCGCGCGCGATGCGCC
AGGCCACCGCGCCGATGGTCGATGTGGTGGCATTGTTTCGCAACAATTCGAAAGAAGAAGTGCGCGAAGTGGTGCGCACC
GTGCGCCCGACCCTGTTGCAGTTCCATGGCGAGGAAGACGACGCGTTCTGCCGTAGCTTCAACCTGCCGTATCTCAAGGC
GGTGCCGATGGGCAGCAGCGGCGTGAATGGCGAAGACGCCAACGCGCGCACGCTGCAACTGTCCTATCCCAATACCGCGG
GTTTTCTGTTCGACAGCCATGCGCCCGGCGCTGGCGGCGGCACCGGCAAGACCTTCGACTGGTCGCGCTTGCCCACCGGC
TTGCATCGTCCGTTCCTGTTGGCCGGTGGCATCAACGCCGACAACGTGTTCGACGCCATCGTGGCCACGTTGCCCTGGGG
CGTGGATGTTTCCAGTGGCGTGGAGTTGGCGCCTGGCATCAAGGACGGCCACAAGATGCGCAAGTTCGTCGAGGAAGTAC
GGCGGGCGGATTGTCACGAGATGTCGTGA

Upstream 100 bases:

>100_bases
GCGACCGCACTGTGGCCGGCCCGACAGCGCCGCCACAAGGTCTGGTATTTATCGGACCTCTGTATCCTGCAGAGTGGCAT
CTGCCTGCTGAGGTGACCCA

Downstream 100 bases:

>100_bases
AGGTGGGGGTAGGGATTCGGGATTCGCAGCAGCGGCTACGCCAAGGCGTCTGAGCGCTGCTTGTTGCGCGCAGCGCCGCT
GCGTCTGGGTCCACTGGGCA

Product: N-(5'-phosphoribosyl)anthranilate isomerase

Products: NA

Alternate protein names: PRAI

Number of amino acids: Translated: 222; Mature: 222

Protein sequence:

>222_residues
MNRSLYRTRIKFCGMTRAGDIRLAGELGVDAVGFIFAHGSPRRVAPAEARAMRQATAPMVDVVALFRNNSKEEVREVVRT
VRPTLLQFHGEEDDAFCRSFNLPYLKAVPMGSSGVNGEDANARTLQLSYPNTAGFLFDSHAPGAGGGTGKTFDWSRLPTG
LHRPFLLAGGINADNVFDAIVATLPWGVDVSSGVELAPGIKDGHKMRKFVEEVRRADCHEMS

Sequences:

>Translated_222_residues
MNRSLYRTRIKFCGMTRAGDIRLAGELGVDAVGFIFAHGSPRRVAPAEARAMRQATAPMVDVVALFRNNSKEEVREVVRT
VRPTLLQFHGEEDDAFCRSFNLPYLKAVPMGSSGVNGEDANARTLQLSYPNTAGFLFDSHAPGAGGGTGKTFDWSRLPTG
LHRPFLLAGGINADNVFDAIVATLPWGVDVSSGVELAPGIKDGHKMRKFVEEVRRADCHEMS
>Mature_222_residues
MNRSLYRTRIKFCGMTRAGDIRLAGELGVDAVGFIFAHGSPRRVAPAEARAMRQATAPMVDVVALFRNNSKEEVREVVRT
VRPTLLQFHGEEDDAFCRSFNLPYLKAVPMGSSGVNGEDANARTLQLSYPNTAGFLFDSHAPGAGGGTGKTFDWSRLPTG
LHRPFLLAGGINADNVFDAIVATLPWGVDVSSGVELAPGIKDGHKMRKFVEEVRRADCHEMS

Specific function: Bifunctional Enzyme That Catalyzes Two Sequential Steps Of Tryptophan Biosynthetic Pathway. The First Reaction Is Catalyzed By The Isomerase, Coded By The Trpf Domain; The Second Reaction Is Catalyzed By The Synthase, Coded By The Trpc Domain. [C]

COG id: COG0135

COG function: function code E; Phosphoribosylanthranilate isomerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpF family

Homologues:

Organism=Escherichia coli, GI87081863, Length=204, Percent_Identity=31.3725490196078, Blast_Score=84, Evalue=5e-18,
Organism=Saccharomyces cerevisiae, GI6320210, Length=224, Percent_Identity=30.8035714285714, Blast_Score=82, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRPF_XANAC (Q8PJ26)

Other databases:

- EMBL:   AE008923
- RefSeq:   NP_643028.1
- ProteinModelPortal:   Q8PJ26
- SMR:   Q8PJ26
- GeneID:   1156790
- GenomeReviews:   AE008923_GR
- KEGG:   xac:XAC2719
- NMPDR:   fig|190486.1.peg.2672
- HOGENOM:   HBG554803
- OMA:   SKICGIT
- ProtClustDB:   PRK01222
- BioCyc:   XAXO190486:XAC2719-MONOMER
- BRENDA:   5.3.1.24
- HAMAP:   MF_00135_B
- InterPro:   IPR013785
- InterPro:   IPR001240
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70

Pfam domain/function: PF00697 PRAI; SSF51366 RibP_bind_barrel

EC number: =5.3.1.24

Molecular weight: Translated: 24149; Mature: 24149

Theoretical pI: Translated: 8.50; Mature: 8.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRSLYRTRIKFCGMTRAGDIRLAGELGVDAVGFIFAHGSPRRVAPAEARAMRQATAPMV
CCCHHHHHHHHHHCCCCCCCEEEECCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHCHHH
DVVALFRNNSKEEVREVVRTVRPTLLQFHGEEDDAFCRSFNLPYLKAVPMGSSGVNGEDA
HHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCEEEECCCCCCCCCCCC
NARTLQLSYPNTAGFLFDSHAPGAGGGTGKTFDWSRLPTGLHRPFLLAGGINADNVFDAI
CCEEEEEECCCCCCEEEECCCCCCCCCCCCCCCHHHCCCCCCCCEEEECCCCHHHHHHHH
VATLPWGVDVSSGVELAPGIKDGHKMRKFVEEVRRADCHEMS
HHHCCCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNRSLYRTRIKFCGMTRAGDIRLAGELGVDAVGFIFAHGSPRRVAPAEARAMRQATAPMV
CCCHHHHHHHHHHCCCCCCCEEEECCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHCHHH
DVVALFRNNSKEEVREVVRTVRPTLLQFHGEEDDAFCRSFNLPYLKAVPMGSSGVNGEDA
HHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCEEEECCCCCCCCCCCC
NARTLQLSYPNTAGFLFDSHAPGAGGGTGKTFDWSRLPTGLHRPFLLAGGINADNVFDAI
CCEEEEEECCCCCCEEEECCCCCCCCCCCCCCCHHHCCCCCCCCEEEECCCCHHHHHHHH
VATLPWGVDVSSGVELAPGIKDGHKMRKFVEEVRRADCHEMS
HHHCCCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217