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The map label for this gene is rsmD [H]

Identifier: 21243260

GI number: 21243260

Start: 2959742

End: 2960365

Strand: Reverse

Name: rsmD [H]

Synonym: XAC2527

Alternate gene names: 21243260

Gene position: 2960365-2959742 (Counterclockwise)

Preceding gene: 21243263

Following gene: 21243259

Centisome position: 57.2

GC content: 73.72

Gene sequence:

>624_bases
ATGAGCCGCCCGCAGCGTCCGGCGCCGCGTGGCGCGGAAGGGCAGGTGCGCATCGTGGGCGGGCGCTGGCGCAATACGCG
GCTGGCAGTGCCCAGCTTGCCTGGCCTGCGCCCCAGCAGCGACCGGGTGCGCGAAACGGTGTTCAACTGGCTGATGCCGC
GCCTGCCCGGCGCGCGCGTGCTGGATCTGTTCGCCGGCTCGGGCGCGCTGGGTCTGGAGGCAGTGTCGCGCGGCGCCGCG
CATGCCACCCTGATCGAACGCGACCCCGGCCTGGTGCAGCGGCTGCGCGAGCACGTGACCCGGCTGGATGCCGCCACGCA
GGTCCAGGTGCTGCAGGAGGATGCGGTGCGCTGGCTGGAGCGTGCGCCCGCAGCGTTGGCCGATATCGTGTTCGTCGACC
CGCCGTTCGCGGCCGGCCTGTGGCCGGCTGTGCTGGAGCGCCTGCCGGCGCATGTGGCTGCCGACGCCTGGCTCTACCTG
GAAGCGCCCGCCGATGCGCCGCCGCTGCTGCCGGCCGGCTGGCACCTGCACCGCGAGGGCGCCACCCGTGAGGTGCGCTA
TGCGCTGTACCGCCGGGCCGCTGCTACACTGAAGAGCGATCCGACCCCGGTAGTCTCCGTATGA

Upstream 100 bases:

>100_bases
GGCGGCGGTATCATGGCCGGCCGTTCGTCCTGCCTTGAGCCATGTCGCCAACTGCGCTGCTGCCGTGCTGTACCCGCCCG
CCCTCGACAGGTGCGTGGCG

Downstream 100 bases:

>100_bases
GCGTTGCCAATAGCCGCACCGCCGTCTATCCCGGTACCTTCGACCCGATCACCAATGGGCATATCGACCTGGTGAATCGC
GCCGCGCCGTTGTTCGAGCG

Product: hypothetical protein

Products: NA

Alternate protein names: 16S rRNA m2G966 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase [H]

Number of amino acids: Translated: 207; Mature: 206

Protein sequence:

>207_residues
MSRPQRPAPRGAEGQVRIVGGRWRNTRLAVPSLPGLRPSSDRVRETVFNWLMPRLPGARVLDLFAGSGALGLEAVSRGAA
HATLIERDPGLVQRLREHVTRLDAATQVQVLQEDAVRWLERAPAALADIVFVDPPFAAGLWPAVLERLPAHVAADAWLYL
EAPADAPPLLPAGWHLHREGATREVRYALYRRAAATLKSDPTPVVSV

Sequences:

>Translated_207_residues
MSRPQRPAPRGAEGQVRIVGGRWRNTRLAVPSLPGLRPSSDRVRETVFNWLMPRLPGARVLDLFAGSGALGLEAVSRGAA
HATLIERDPGLVQRLREHVTRLDAATQVQVLQEDAVRWLERAPAALADIVFVDPPFAAGLWPAVLERLPAHVAADAWLYL
EAPADAPPLLPAGWHLHREGATREVRYALYRRAAATLKSDPTPVVSV
>Mature_206_residues
SRPQRPAPRGAEGQVRIVGGRWRNTRLAVPSLPGLRPSSDRVRETVFNWLMPRLPGARVLDLFAGSGALGLEAVSRGAAH
ATLIERDPGLVQRLREHVTRLDAATQVQVLQEDAVRWLERAPAALADIVFVDPPFAAGLWPAVLERLPAHVAADAWLYLE
APADAPPLLPAGWHLHREGATREVRYALYRRAAATLKSDPTPVVSV

Specific function: Specifically methylates the guanosine in position 966 of 16S rRNA in the assembled 30S particle [H]

COG id: COG0742

COG function: function code L; N6-adenine-specific methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmD family [H]

Homologues:

Organism=Escherichia coli, GI1789875, Length=193, Percent_Identity=46.1139896373057, Blast_Score=156, Evalue=1e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004398 [H]

Pfam domain/function: PF03602 Cons_hypoth95 [H]

EC number: =2.1.1.171 [H]

Molecular weight: Translated: 22619; Mature: 22488

Theoretical pI: Translated: 10.99; Mature: 10.99

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRPQRPAPRGAEGQVRIVGGRWRNTRLAVPSLPGLRPSSDRVRETVFNWLMPRLPGARV
CCCCCCCCCCCCCCCEEEECCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCHH
LDLFAGSGALGLEAVSRGAAHATLIERDPGLVQRLREHVTRLDAATQVQVLQEDAVRWLE
HHHHCCCCCHHHHHHHCCCHHEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RAPAALADIVFVDPPFAAGLWPAVLERLPAHVAADAWLYLEAPADAPPLLPAGWHLHREG
HCCHHHHHEEEECCCHHHHHHHHHHHHCCHHHHCCEEEEEECCCCCCCCCCCCCEEECCC
ATREVRYALYRRAAATLKSDPTPVVSV
CHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SRPQRPAPRGAEGQVRIVGGRWRNTRLAVPSLPGLRPSSDRVRETVFNWLMPRLPGARV
CCCCCCCCCCCCCCEEEECCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCHH
LDLFAGSGALGLEAVSRGAAHATLIERDPGLVQRLREHVTRLDAATQVQVLQEDAVRWLE
HHHHCCCCCHHHHHHHCCCHHEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RAPAALADIVFVDPPFAAGLWPAVLERLPAHVAADAWLYLEAPADAPPLLPAGWHLHREG
HCCHHHHHEEEECCCHHHHHHHHHHHHCCHHHHCCEEEEEECCCCCCCCCCCCCEEECCC
ATREVRYALYRRAAATLKSDPTPVVSV
CHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]