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The map label for this gene is ndhF [H]

Identifier: 21242907

GI number: 21242907

Start: 2543779

End: 2545350

Strand: Direct

Name: ndhF [H]

Synonym: XAC2172

Alternate gene names: 21242907

Gene position: 2543779-2545350 (Clockwise)

Preceding gene: 21242905

Following gene: 77748635

Centisome position: 49.15

GC content: 68.51

Gene sequence:

>1572_bases
TTGTCGCTCTATCTACTCCCACTGTTCGCACCACTCCTGCTGCTGACCGCCGCTGTCGTCGCCCTGGTTCGCGGCGGTCG
CCGTCCACTCAAGATCCCCCTCCTCGCAGAAGGCGCCGCGCTCGGCGCGCTCTGCATCGCGATCGCATCGCTGGCGCTGG
TGCTGTCGCGAGGCACTGGCGACAGCCCACTGATCGGATGGGGCGACATCGGCCTGTCCGCGCGGCTCGACGCGGTCAGC
GTGACGATGCTTTTGCTGGTGACCTTCGTTGGTTGGATCGTGGTGCGGTACGCACGCACCTATCTCGACGGCGAGGCTCG
CCAGGGCTACTTCACCGGCTGGCTATGCATGGCACTGGCGGCGGTGCTGCTGCTGGTGCAGGCAGGCAATCTGATCCAGG
TCGTCATCGCCTGGATTGCCACCAGCATCGCGATGCATCGCCTGCTGCTGTTCTATCCCGAGCGCGTGGAAGCGCAGCGC
GCCGCACGTAAGAAGCGCGTGTTCTCCACCACCGGCGGTATCGCCCTGACCTGCGGCGCGCTGCTGATCTGGAAAAGCAT
GGGCACGACCGATATCGCCACGATCAACGCGATGGCCGCGCGCGGGCAGCACTCCTGGTCGCTGGTCGCGGCGGCCGCTC
TGCTTGCACTTGCTGCGGTCTTGAAATCGGCGCAGTTCCCGACCCACGGCTGGCTCACCGAGATGATGGAAGCGCCCACG
CCGGTATCGGCCTTGCTGCACGCCGGCGTGGTCAATGGCGGCGGCTTCCTGCTGATCCGCTTTGCCGACCTGATGCTTGC
CGCCCCCGGCGTGCTCGCCGTGCTGGCCATGCTGGGCGGGTTCACCGCCTTGTTCGGCGCGCTGGTGATGCTGACCCAGC
CGGCGGTCAAGACCTCGCTCGCCTGGTCCACGGTCGCGCAGATGGGATTCATGATGCTCCAGTGCGGTCTGGCGCTGTTC
CCGCTGGCCCTGCTGCATATCGTGGCGCACTCGCTCTACAAGGCGCACGCGTTCCTGGCCTCCGGCGGCGCGGTCGAGCA
GGTGGCGGCCATTCGTCGGCCCGGGCCGGTCGCCGTTCCGGATGGGTCGGCGGTCGGCCGCGCATTTTTGATCGCACTGG
CGATCTATATCGGCATCGGCACTGGCTTCGGGTCCGCCTTCGGCTTCGAGCACAAGTCGCCGCAGGCGCTGGCGCTGGGC
GCGATCCTGATCTTCGGGGTCGCCTATCTGCTGGCCCAGGGCCTTGCCGACGCCGCGCCGCGCCCCCTCACCCGCAGGAC
GGCGATCTACGCCAGCGCCGCGGCGATCGGCTACTTCGCGCTGCAGACCACCGCCGAATGGCTGACCGCCGGCGTGCTGC
CGGCAACGCCGGCGCCGGGACGCCTGGAGTGGACCCTGATGGCGCTCGCGGTGCTGAGCTTCGGGCTGGTGGCGGTCGCG
CAAGCGCTGTTTCCGCTCTGGGCATCGCACCCCGCTGCCGCCGGGCTCCGCGTCCACCTCTCCAATGGGCTGTACGCCAA
CGCCACGATCGATCGCCTGCTTGGCGGCTGGTCCGTTCGCAAGACCTCCTGA

Upstream 100 bases:

>100_bases
TAATAACGAACGAATCTGCAATGACAATGAATTTTTATTCAACCCCACCGGTCGTTATCGTGGCGCGGCCCGCCACCACG
TTCGTTCTTAGGGGATCGCC

Downstream 100 bases:

>100_bases
ACCCCTTCACGCCAGGCCCCCATGCTGATGACCACGACGACCACCGCCATGTCTGTGTCCCTGTCCCACGATGTGATCAT
CGCCGCCGCCCAACGTGCGG

Product: NADH dehydrogenase

Products: NAD+; ubiquinol [C]

Alternate protein names: NAD(P)H dehydrogenase I, chain 5; NDH-1, chain 5 [H]

Number of amino acids: Translated: 523; Mature: 522

Protein sequence:

>523_residues
MSLYLLPLFAPLLLLTAAVVALVRGGRRPLKIPLLAEGAALGALCIAIASLALVLSRGTGDSPLIGWGDIGLSARLDAVS
VTMLLLVTFVGWIVVRYARTYLDGEARQGYFTGWLCMALAAVLLLVQAGNLIQVVIAWIATSIAMHRLLLFYPERVEAQR
AARKKRVFSTTGGIALTCGALLIWKSMGTTDIATINAMAARGQHSWSLVAAAALLALAAVLKSAQFPTHGWLTEMMEAPT
PVSALLHAGVVNGGGFLLIRFADLMLAAPGVLAVLAMLGGFTALFGALVMLTQPAVKTSLAWSTVAQMGFMMLQCGLALF
PLALLHIVAHSLYKAHAFLASGGAVEQVAAIRRPGPVAVPDGSAVGRAFLIALAIYIGIGTGFGSAFGFEHKSPQALALG
AILIFGVAYLLAQGLADAAPRPLTRRTAIYASAAAIGYFALQTTAEWLTAGVLPATPAPGRLEWTLMALAVLSFGLVAVA
QALFPLWASHPAAAGLRVHLSNGLYANATIDRLLGGWSVRKTS

Sequences:

>Translated_523_residues
MSLYLLPLFAPLLLLTAAVVALVRGGRRPLKIPLLAEGAALGALCIAIASLALVLSRGTGDSPLIGWGDIGLSARLDAVS
VTMLLLVTFVGWIVVRYARTYLDGEARQGYFTGWLCMALAAVLLLVQAGNLIQVVIAWIATSIAMHRLLLFYPERVEAQR
AARKKRVFSTTGGIALTCGALLIWKSMGTTDIATINAMAARGQHSWSLVAAAALLALAAVLKSAQFPTHGWLTEMMEAPT
PVSALLHAGVVNGGGFLLIRFADLMLAAPGVLAVLAMLGGFTALFGALVMLTQPAVKTSLAWSTVAQMGFMMLQCGLALF
PLALLHIVAHSLYKAHAFLASGGAVEQVAAIRRPGPVAVPDGSAVGRAFLIALAIYIGIGTGFGSAFGFEHKSPQALALG
AILIFGVAYLLAQGLADAAPRPLTRRTAIYASAAAIGYFALQTTAEWLTAGVLPATPAPGRLEWTLMALAVLSFGLVAVA
QALFPLWASHPAAAGLRVHLSNGLYANATIDRLLGGWSVRKTS
>Mature_522_residues
SLYLLPLFAPLLLLTAAVVALVRGGRRPLKIPLLAEGAALGALCIAIASLALVLSRGTGDSPLIGWGDIGLSARLDAVSV
TMLLLVTFVGWIVVRYARTYLDGEARQGYFTGWLCMALAAVLLLVQAGNLIQVVIAWIATSIAMHRLLLFYPERVEAQRA
ARKKRVFSTTGGIALTCGALLIWKSMGTTDIATINAMAARGQHSWSLVAAAALLALAAVLKSAQFPTHGWLTEMMEAPTP
VSALLHAGVVNGGGFLLIRFADLMLAAPGVLAVLAMLGGFTALFGALVMLTQPAVKTSLAWSTVAQMGFMMLQCGLALFP
LALLHIVAHSLYKAHAFLASGGAVEQVAAIRRPGPVAVPDGSAVGRAFLIALAIYIGIGTGFGSAFGFEHKSPQALALGA
ILIFGVAYLLAQGLADAAPRPLTRRTAIYASAAAIGYFALQTTAEWLTAGVLPATPAPGRLEWTLMALAVLSFGLVAVAQ
ALFPLWASHPAAAGLRVHLSNGLYANATIDRLLGGWSVRKTS

Specific function: NDH-1 shuttles electrons from NAD(P)H, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone. Couples the redox reaction to proton tran

COG id: COG1009

COG function: function code CP; NADH:ubiquinone oxidoreductase subunit 5 (chain L)/Multisubunit Na+/H+ antiporter, MnhA subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=296, Percent_Identity=33.445945945946, Blast_Score=135, Evalue=7e-32,
Organism=Escherichia coli, GI1788614, Length=297, Percent_Identity=34.006734006734, Blast_Score=142, Evalue=5e-35,
Organism=Escherichia coli, GI1788829, Length=327, Percent_Identity=30.2752293577982, Blast_Score=93, Evalue=5e-20,
Organism=Escherichia coli, GI1788831, Length=298, Percent_Identity=29.1946308724832, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1788827, Length=302, Percent_Identity=25.4966887417219, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR001516
- InterPro:   IPR002128
- InterPro:   IPR003945
- InterPro:   IPR018393 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF01010 Oxidored_q1_C; PF00662 Oxidored_q1_N [H]

EC number: 1.6.99.5 [C]

Molecular weight: Translated: 54558; Mature: 54426

Theoretical pI: Translated: 10.40; Mature: 10.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLYLLPLFAPLLLLTAAVVALVRGGRRPLKIPLLAEGAALGALCIAIASLALVLSRGTG
CCEEEHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHCCCC
DSPLIGWGDIGLSARLDAVSVTMLLLVTFVGWIVVRYARTYLDGEARQGYFTGWLCMALA
CCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
AVLLLVQAGNLIQVVIAWIATSIAMHRLLLFYPERVEAQRAARKKRVFSTTGGIALTCGA
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHH
LLIWKSMGTTDIATINAMAARGQHSWSLVAAAALLALAAVLKSAQFPTHGWLTEMMEAPT
HHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCC
PVSALLHAGVVNGGGFLLIRFADLMLAAPGVLAVLAMLGGFTALFGALVMLTQPAVKTSL
HHHHHHHHCCCCCCCEEHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
AWSTVAQMGFMMLQCGLALFPLALLHIVAHSLYKAHAFLASGGAVEQVAAIRRPGPVAVP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCC
DGSAVGRAFLIALAIYIGIGTGFGSAFGFEHKSPQALALGAILIFGVAYLLAQGLADAAP
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
RPLTRRTAIYASAAAIGYFALQTTAEWLTAGVLPATPAPGRLEWTLMALAVLSFGLVAVA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
QALFPLWASHPAAAGLRVHLSNGLYANATIDRLLGGWSVRKTS
HHHHHHHHCCCCCCCEEEEECCCEEEHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SLYLLPLFAPLLLLTAAVVALVRGGRRPLKIPLLAEGAALGALCIAIASLALVLSRGTG
CEEEHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHCCCC
DSPLIGWGDIGLSARLDAVSVTMLLLVTFVGWIVVRYARTYLDGEARQGYFTGWLCMALA
CCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
AVLLLVQAGNLIQVVIAWIATSIAMHRLLLFYPERVEAQRAARKKRVFSTTGGIALTCGA
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHH
LLIWKSMGTTDIATINAMAARGQHSWSLVAAAALLALAAVLKSAQFPTHGWLTEMMEAPT
HHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCC
PVSALLHAGVVNGGGFLLIRFADLMLAAPGVLAVLAMLGGFTALFGALVMLTQPAVKTSL
HHHHHHHHCCCCCCCEEHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
AWSTVAQMGFMMLQCGLALFPLALLHIVAHSLYKAHAFLASGGAVEQVAAIRRPGPVAVP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCC
DGSAVGRAFLIALAIYIGIGTGFGSAFGFEHKSPQALALGAILIFGVAYLLAQGLADAAP
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
RPLTRRTAIYASAAAIGYFALQTTAEWLTAGVLPATPAPGRLEWTLMALAVLSFGLVAVA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
QALFPLWASHPAAAGLRVHLSNGLYANATIDRLLGGWSVRKTS
HHHHHHHHCCCCCCCEEEEECCCEEEHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: Iron [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; ubiquinone [C]

Specific reaction: NADH + ubiquinone = NAD+ + ubiquinol [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]