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The map label for this gene is fleQ [H]

Identifier: 21242711

GI number: 21242711

Start: 2298597

End: 2300081

Strand: Reverse

Name: fleQ [H]

Synonym: XAC1967

Alternate gene names: 21242711

Gene position: 2300081-2298597 (Counterclockwise)

Preceding gene: 21242712

Following gene: 21242710

Centisome position: 44.44

GC content: 66.4

Gene sequence:

>1485_bases
ATGAGTGAGTCCCGCATTCTGTTGATCGACAGTGACGCCGTGCGCGCCGAGCGCACCGTGTCTCTGCTTGAGTTCATGGA
CTTCAATCCCCGTTGGGTCACCGACGGCGCCGACATCAATCCTGGCCGTCACCGCCACGATGAGTGGATGGCGGTGATGG
TGGGCTCGGCGCAGGACGCCGCACAGGCCGACAAATTCTTCGACTGGCTGGCCGATGCCAAGCTGCCGCCACCGGTGTTG
CTGATGGAGGGCAGCCCGAGCGCGTTTGCGCAGACCCACGGCCTGCACGAAGCCAATGTGTGGGCGTTGGACACTCCCCT
GCGTCATGCGCAGCTGGAAGCGTTGCTGCGCCGCGCAAGCCTCAAGCGGCTGGACGCCGAGCATCAGGCCGGCGTGCAGC
AGGACTCCGGCCCAACCGGCAACAGCGAGGCGGTGACCCGCCTGCGCCGGCTGATCGACCAGGTGGCCGCATTCGACACC
ACAGTGCTGGTGCTGGGCGAATCCGGCACCGGCAAGGAAGTGGTGGCACGCGCCATCCACCAGCACTCGCCGCGGCGCGA
CGGGCCGTTCGTGGCGATCAATTGCGGCGCGATCCCGCCGGATCTGCTGGAAAGCGAATTGTTCGGCCACGAAAAAGGCG
CCTTCACCGGTGCGCTGACCACCCGCAAGGGTCGCTTCGAGATGGCCGAAGGCGGCACGCTGCTGCTGGACGAAATTGGC
GACATGAGCCTGCCGATGCAGGTCAAGTTGTTGCGCGTGCTGCAGGAGCGCAGCTTCGAGCGCGTCGGCGGCGGGCAGAC
CATCCGCTGCAACGTGCGCGTGATCGCCGCGACCCACCGCAATCTTGAATCGCGCATCAGCGATGGCCAGTTCCGCGAGG
ACCTGTTCTATCGCCTCAATGTGTTTCCGATCGAAATGCCGGCACTGCGCGAGCGCGTGGACGATCTGGCCATGCTGGTG
CAGACCATTGCAGGCCAGCTGGCACGCACCGGTCGCGGCGAAGTGCGCTTTGCCGACGAGGCCCTGCAGGCGCTGCGCAG
CTACGATTGGCCAGGCAATGTGCGCGAGCTGACCAACCTGGTCGAGCGCCTGGCGGTGCTGCATCCCGGCGGGCTGGTGC
GGGTGCAGGATCTGCCGGCGCGTTATCGCGGCGATTTCGCTTCGGCCATTCCGGTCGAGCTGCCGCCGGAGCCGGAGTTG
GTGGCCGCACCGGTGGAGGTCAGCGCACTGCCGAGCAATGTGGTGACGCTGCAACCGAAGACCGCCGATGCCGAGCCGGC
GGCGACGTCCAGCCTGCCGGACGACGGCATCGATCTGCGTGGGCATATGGCCAATATCGAGTTGGCGTTGATCAACGAAG
CATTGGAGCGCACGCAGGGCGTGGTGGCGCATGCCGCTCAGCTGCTGGGCCTGCGCCGCACCACGTTGGTGGAGAAGCTG
CGCAAGTACGGCATCGATCGCGAGCAGACCGAGCTGGCCAACTGA

Upstream 100 bases:

>100_bases
GAACACGCTCTTCGCGCGGGTGCTGACAATTTTGTCAGCAAGCTGTCCTATATCCAGGAAGTCATGCCAATCCTGGAGGG
CCTGACGGAAGGAGCCAGGA

Downstream 100 bases:

>100_bases
GCCAGCACGCGCTCAGGTTCGGCTCGCTGGCATAGCGCTTGCTTCAGCAGGATCACTCCGTTCGCAGCCGCCGGCTGCGA
ACGGAATCCCGACCTTCACC

Product: transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 494; Mature: 493

Protein sequence:

>494_residues
MSESRILLIDSDAVRAERTVSLLEFMDFNPRWVTDGADINPGRHRHDEWMAVMVGSAQDAAQADKFFDWLADAKLPPPVL
LMEGSPSAFAQTHGLHEANVWALDTPLRHAQLEALLRRASLKRLDAEHQAGVQQDSGPTGNSEAVTRLRRLIDQVAAFDT
TVLVLGESGTGKEVVARAIHQHSPRRDGPFVAINCGAIPPDLLESELFGHEKGAFTGALTTRKGRFEMAEGGTLLLDEIG
DMSLPMQVKLLRVLQERSFERVGGGQTIRCNVRVIAATHRNLESRISDGQFREDLFYRLNVFPIEMPALRERVDDLAMLV
QTIAGQLARTGRGEVRFADEALQALRSYDWPGNVRELTNLVERLAVLHPGGLVRVQDLPARYRGDFASAIPVELPPEPEL
VAAPVEVSALPSNVVTLQPKTADAEPAATSSLPDDGIDLRGHMANIELALINEALERTQGVVAHAAQLLGLRRTTLVEKL
RKYGIDREQTELAN

Sequences:

>Translated_494_residues
MSESRILLIDSDAVRAERTVSLLEFMDFNPRWVTDGADINPGRHRHDEWMAVMVGSAQDAAQADKFFDWLADAKLPPPVL
LMEGSPSAFAQTHGLHEANVWALDTPLRHAQLEALLRRASLKRLDAEHQAGVQQDSGPTGNSEAVTRLRRLIDQVAAFDT
TVLVLGESGTGKEVVARAIHQHSPRRDGPFVAINCGAIPPDLLESELFGHEKGAFTGALTTRKGRFEMAEGGTLLLDEIG
DMSLPMQVKLLRVLQERSFERVGGGQTIRCNVRVIAATHRNLESRISDGQFREDLFYRLNVFPIEMPALRERVDDLAMLV
QTIAGQLARTGRGEVRFADEALQALRSYDWPGNVRELTNLVERLAVLHPGGLVRVQDLPARYRGDFASAIPVELPPEPEL
VAAPVEVSALPSNVVTLQPKTADAEPAATSSLPDDGIDLRGHMANIELALINEALERTQGVVAHAAQLLGLRRTTLVEKL
RKYGIDREQTELAN
>Mature_493_residues
SESRILLIDSDAVRAERTVSLLEFMDFNPRWVTDGADINPGRHRHDEWMAVMVGSAQDAAQADKFFDWLADAKLPPPVLL
MEGSPSAFAQTHGLHEANVWALDTPLRHAQLEALLRRASLKRLDAEHQAGVQQDSGPTGNSEAVTRLRRLIDQVAAFDTT
VLVLGESGTGKEVVARAIHQHSPRRDGPFVAINCGAIPPDLLESELFGHEKGAFTGALTTRKGRFEMAEGGTLLLDEIGD
MSLPMQVKLLRVLQERSFERVGGGQTIRCNVRVIAATHRNLESRISDGQFREDLFYRLNVFPIEMPALRERVDDLAMLVQ
TIAGQLARTGRGEVRFADEALQALRSYDWPGNVRELTNLVERLAVLHPGGLVRVQDLPARYRGDFASAIPVELPPEPELV
AAPVEVSALPSNVVTLQPKTADAEPAATSSLPDDGIDLRGHMANIELALINEALERTQGVVAHAAQLLGLRRTTLVEKLR
KYGIDREQTELAN

Specific function: Required for induction of expression of the hydrogenase- 4 structural genes [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI87082117, Length=349, Percent_Identity=47.8510028653295, Blast_Score=285, Evalue=3e-78,
Organism=Escherichia coli, GI1788550, Length=421, Percent_Identity=39.1923990498812, Blast_Score=271, Evalue=7e-74,
Organism=Escherichia coli, GI1789087, Length=352, Percent_Identity=43.1818181818182, Blast_Score=268, Evalue=8e-73,
Organism=Escherichia coli, GI1790299, Length=415, Percent_Identity=40.9638554216867, Blast_Score=264, Evalue=9e-72,
Organism=Escherichia coli, GI1790437, Length=408, Percent_Identity=40.4411764705882, Blast_Score=264, Evalue=1e-71,
Organism=Escherichia coli, GI1788905, Length=349, Percent_Identity=41.8338108882521, Blast_Score=247, Evalue=1e-66,
Organism=Escherichia coli, GI1789233, Length=347, Percent_Identity=42.3631123919308, Blast_Score=233, Evalue=3e-62,
Organism=Escherichia coli, GI87082152, Length=267, Percent_Identity=48.314606741573, Blast_Score=230, Evalue=1e-61,
Organism=Escherichia coli, GI1786524, Length=242, Percent_Identity=48.7603305785124, Blast_Score=209, Evalue=3e-55,
Organism=Escherichia coli, GI87081872, Length=351, Percent_Identity=35.3276353276353, Blast_Score=202, Evalue=4e-53,
Organism=Escherichia coli, GI1787583, Length=397, Percent_Identity=33.7531486146096, Blast_Score=185, Evalue=7e-48,
Organism=Escherichia coli, GI1789828, Length=342, Percent_Identity=31.5789473684211, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI87081858, Length=344, Percent_Identity=30.2325581395349, Blast_Score=137, Evalue=1e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 54314; Mature: 54183

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSESRILLIDSDAVRAERTVSLLEFMDFNPRWVTDGADINPGRHRHDEWMAVMVGSAQDA
CCCCEEEEEECCCHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCEEEEEECCCHHH
AQADKFFDWLADAKLPPPVLLMEGSPSAFAQTHGLHEANVWALDTPLRHAQLEALLRRAS
HHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHHH
LKRLDAEHQAGVQQDSGPTGNSEAVTRLRRLIDQVAAFDTTVLVLGESGTGKEVVARAIH
HHHHCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHH
QHSPRRDGPFVAINCGAIPPDLLESELFGHEKGAFTGALTTRKGRFEMAEGGTLLLDEIG
HCCCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCEEEEEECCCCCEEECCCCEEEEECCC
DMSLPMQVKLLRVLQERSFERVGGGQTIRCNVRVIAATHRNLESRISDGQFREDLFYRLN
CCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEEHHHCHHHHCCCCHHHHHHHEEEE
VFPIEMPALRERVDDLAMLVQTIAGQLARTGRGEVRFADEALQALRSYDWPGNVRELTNL
EEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHCCCCCCCHHHHHHH
VERLAVLHPGGLVRVQDLPARYRGDFASAIPVELPPEPELVAAPVEVSALPSNVVTLQPK
HHHHHHCCCCCEEEEECCCHHHCCCCCCCCCCCCCCCCCEEECCCEEECCCCCEEEECCC
TADAEPAATSSLPDDGIDLRGHMANIELALINEALERTQGVVAHAAQLLGLRRTTLVEKL
CCCCCCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RKYGIDREQTELAN
HHHCCCHHHHHCCC
>Mature Secondary Structure 
SESRILLIDSDAVRAERTVSLLEFMDFNPRWVTDGADINPGRHRHDEWMAVMVGSAQDA
CCCEEEEEECCCHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCEEEEEECCCHHH
AQADKFFDWLADAKLPPPVLLMEGSPSAFAQTHGLHEANVWALDTPLRHAQLEALLRRAS
HHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHHH
LKRLDAEHQAGVQQDSGPTGNSEAVTRLRRLIDQVAAFDTTVLVLGESGTGKEVVARAIH
HHHHCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHH
QHSPRRDGPFVAINCGAIPPDLLESELFGHEKGAFTGALTTRKGRFEMAEGGTLLLDEIG
HCCCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCEEEEEECCCCCEEECCCCEEEEECCC
DMSLPMQVKLLRVLQERSFERVGGGQTIRCNVRVIAATHRNLESRISDGQFREDLFYRLN
CCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEEHHHCHHHHCCCCHHHHHHHEEEE
VFPIEMPALRERVDDLAMLVQTIAGQLARTGRGEVRFADEALQALRSYDWPGNVRELTNL
EEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHCCCCCCCHHHHHHH
VERLAVLHPGGLVRVQDLPARYRGDFASAIPVELPPEPELVAAPVEVSALPSNVVTLQPK
HHHHHHCCCCCEEEEECCCHHHCCCCCCCCCCCCCCCCCEEECCCEEECCCCCEEEECCC
TADAEPAATSSLPDDGIDLRGHMANIELALINEALERTQGVVAHAAQLLGLRRTTLVEKL
CCCCCCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RKYGIDREQTELAN
HHHCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]