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The map label for this gene is purR [H]

Identifier: 21242306

GI number: 21242306

Start: 1795532

End: 1796638

Strand: Direct

Name: purR [H]

Synonym: XAC1555

Alternate gene names: 21242306

Gene position: 1795532-1796638 (Clockwise)

Preceding gene: 21242305

Following gene: 21242307

Centisome position: 34.69

GC content: 67.39

Gene sequence:

>1107_bases
ATGACGGCGGACGACGCGACGCCGGGCGCGCGGCGCGGCGCGGTGACGTTACGCGATATCGCCGGCGCCATTGGTGTCTC
GCGCGCAACGGTGTCGCTGGTGCTGCGCGGTAGCCCGCTCGTGCATGCGAGCACGCGCGCGCGCGTGGAGGCCGAGCTGG
ATCGGCAGAATTACGTCTACAACCGCGCCGCGGCGAACTTACGCCAGCGCACCTCGTCCAGCGTCGCGCTGGTGATCAAC
GATTTGTCCAACCCGTTTTTTGCCGAGTTCGCCGCCGGCGTCGACGAAGCACTGGGCGCAGCCGGCTATGTCACCTTGCT
CGGTAGCACCGGCGAATCGACGCAGCGTCAACAAGCAGTGCTGACCTCGCTGATGGAGCACACGCCGGCGGGCATCATCC
TGTCGCCGGCCGAAGGCAGCCAGGCGCAGGCGCTGAGCCAGGTGCTCGGCCGCCAGCGCAACGTGGTGCTGTTCAATCGC
GAGGTGGAAGGCGCGCAATGGGATCTGCTGGCGCTCGACAACGCACAAGGCGCGTTCGTGGCCTGCTCGCATCTGATCGC
GCAAGGTCATCGGCGTATCGTGTTCTTCGGTGGGCATGCCGCATCCAGTTCCTGCCGCCAGCGCCGCGCCGGCTACGCGC
GTGCGATGCAGGAGGCGGACCTGGAAACGCATTACGTCGAGTCGGCTCCAAATCGCCAGGACGCCGCAGTCTGCGGGGCG
CGCTTGCTAAACAGTACGGCGCAGGCAGCGACTGCGGCGGTCTGCTACAACGACACCGTTGCGCTCGGCTTGATGGCGGC
ACTGGCCAAGCACGGCCTCGTACCCGGCCGCGACTTCGCGGTGACCGGCTTCGACGATATCGCCGAAGCGGCGCTGTTCA
CGCCTGCATTGACCACCCTGGCTGCAGACCCGCGTGCGCGGGGCAGGCAGGCTGCGCAATTGGTGCTGCAACGCATCGGT
AGCCCCGAGCTTGCCGCACAACAGCTGACCGCCGCAGTCGCACTGAATATTCGTGCGAGCAGCGCCTGCGGACCCACAAC
GCGAATGTCACCACCTCCCCCAAGCGGTCCATCCGCCCATTCCCCCACCAAGGCCTCAGGCCGCTGA

Upstream 100 bases:

>100_bases
GGAAAAGGCCTAATTGGATCGATCCAATTTGATGCCACTGCATGCGTCTTTCTTCCACCGCAAGGCTTGCCACGTGACGC
CAGCCAAGCCGACACCCGAG

Downstream 100 bases:

>100_bases
CCAGGCAATCCCTATGGTTTCCCTTTCCACGCAATCCTCCGTGCCCAGCGGCAACAAGGCGCCCGTCAACAACGGCGTCG
CGTTGTCGGTGGTCACCACG

Product: transcriptional regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 368; Mature: 367

Protein sequence:

>368_residues
MTADDATPGARRGAVTLRDIAGAIGVSRATVSLVLRGSPLVHASTRARVEAELDRQNYVYNRAAANLRQRTSSSVALVIN
DLSNPFFAEFAAGVDEALGAAGYVTLLGSTGESTQRQQAVLTSLMEHTPAGIILSPAEGSQAQALSQVLGRQRNVVLFNR
EVEGAQWDLLALDNAQGAFVACSHLIAQGHRRIVFFGGHAASSSCRQRRAGYARAMQEADLETHYVESAPNRQDAAVCGA
RLLNSTAQAATAAVCYNDTVALGLMAALAKHGLVPGRDFAVTGFDDIAEAALFTPALTTLAADPRARGRQAAQLVLQRIG
SPELAAQQLTAAVALNIRASSACGPTTRMSPPPPSGPSAHSPTKASGR

Sequences:

>Translated_368_residues
MTADDATPGARRGAVTLRDIAGAIGVSRATVSLVLRGSPLVHASTRARVEAELDRQNYVYNRAAANLRQRTSSSVALVIN
DLSNPFFAEFAAGVDEALGAAGYVTLLGSTGESTQRQQAVLTSLMEHTPAGIILSPAEGSQAQALSQVLGRQRNVVLFNR
EVEGAQWDLLALDNAQGAFVACSHLIAQGHRRIVFFGGHAASSSCRQRRAGYARAMQEADLETHYVESAPNRQDAAVCGA
RLLNSTAQAATAAVCYNDTVALGLMAALAKHGLVPGRDFAVTGFDDIAEAALFTPALTTLAADPRARGRQAAQLVLQRIG
SPELAAQQLTAAVALNIRASSACGPTTRMSPPPPSGPSAHSPTKASGR
>Mature_367_residues
TADDATPGARRGAVTLRDIAGAIGVSRATVSLVLRGSPLVHASTRARVEAELDRQNYVYNRAAANLRQRTSSSVALVIND
LSNPFFAEFAAGVDEALGAAGYVTLLGSTGESTQRQQAVLTSLMEHTPAGIILSPAEGSQAQALSQVLGRQRNVVLFNRE
VEGAQWDLLALDNAQGAFVACSHLIAQGHRRIVFFGGHAASSSCRQRRAGYARAMQEADLETHYVESAPNRQDAAVCGAR
LLNSTAQAATAAVCYNDTVALGLMAALAKHGLVPGRDFAVTGFDDIAEAALFTPALTTLAADPRARGRQAAQLVLQRIGS
PELAAQQLTAAVALNIRASSACGPTTRMSPPPPSGPSAHSPTKASGR

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789202, Length=289, Percent_Identity=30.7958477508651, Blast_Score=131, Evalue=8e-32,
Organism=Escherichia coli, GI1790369, Length=337, Percent_Identity=26.7062314540059, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1787906, Length=327, Percent_Identity=31.1926605504587, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI1790194, Length=330, Percent_Identity=29.3939393939394, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI1788474, Length=306, Percent_Identity=29.7385620915033, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1787948, Length=335, Percent_Identity=26.5671641791045, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1786540, Length=349, Percent_Identity=27.7936962750716, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI48994940, Length=326, Percent_Identity=26.0736196319018, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1790715, Length=318, Percent_Identity=24.8427672955975, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1789068, Length=289, Percent_Identity=26.2975778546713, Blast_Score=93, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 38373; Mature: 38241

Theoretical pI: Translated: 8.74; Mature: 8.74

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTADDATPGARRGAVTLRDIAGAIGVSRATVSLVLRGSPLVHASTRARVEAELDRQNYVY
CCCCCCCCCCCCCCEEHHHHHHHHHHHHEEEEEEECCCCCEECCHHHHHHHHHHHHHHHH
NRAAANLRQRTSSSVALVINDLSNPFFAEFAAGVDEALGAAGYVTLLGSTGESTQRQQAV
HHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHH
LTSLMEHTPAGIILSPAEGSQAQALSQVLGRQRNVVLFNREVEGAQWDLLALDNAQGAFV
HHHHHHHCCCCEEEECCCCCHHHHHHHHHCCCCCEEEEECCCCCCEEEEEEECCCCCHHH
ACSHLIAQGHRRIVFFGGHAASSSCRQRRAGYARAMQEADLETHYVESAPNRQDAAVCGA
HHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCHHHHHHHH
RLLNSTAQAATAAVCYNDTVALGLMAALAKHGLVPGRDFAVTGFDDIAEAALFTPALTTL
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCEEEECHHHHHHHHHHHHHHHHH
AADPRARGRQAAQLVLQRIGSPELAAQQLTAAVALNIRASSACGPTTRMSPPPPSGPSAH
HCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHEEEECCCCCCCCCCCCCCCCCCCCCC
SPTKASGR
CCCCCCCC
>Mature Secondary Structure 
TADDATPGARRGAVTLRDIAGAIGVSRATVSLVLRGSPLVHASTRARVEAELDRQNYVY
CCCCCCCCCCCCCEEHHHHHHHHHHHHEEEEEEECCCCCEECCHHHHHHHHHHHHHHHH
NRAAANLRQRTSSSVALVINDLSNPFFAEFAAGVDEALGAAGYVTLLGSTGESTQRQQAV
HHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHH
LTSLMEHTPAGIILSPAEGSQAQALSQVLGRQRNVVLFNREVEGAQWDLLALDNAQGAFV
HHHHHHHCCCCEEEECCCCCHHHHHHHHHCCCCCEEEEECCCCCCEEEEEEECCCCCHHH
ACSHLIAQGHRRIVFFGGHAASSSCRQRRAGYARAMQEADLETHYVESAPNRQDAAVCGA
HHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCHHHHHHHH
RLLNSTAQAATAAVCYNDTVALGLMAALAKHGLVPGRDFAVTGFDDIAEAALFTPALTTL
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCEEEECHHHHHHHHHHHHHHHHH
AADPRARGRQAAQLVLQRIGSPELAAQQLTAAVALNIRASSACGPTTRMSPPPPSGPSAH
HCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHEEEECCCCCCCCCCCCCCCCCCCCCC
SPTKASGR
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA