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The map label for this gene is rumA [H]

Identifier: 21242082

GI number: 21242082

Start: 1534335

End: 1535690

Strand: Direct

Name: rumA [H]

Synonym: XAC1329

Alternate gene names: 21242082

Gene position: 1534335-1535690 (Clockwise)

Preceding gene: 21242080

Following gene: 21242083

Centisome position: 29.65

GC content: 64.97

Gene sequence:

>1356_bases
TTGCCCGCAGCTTGTCGCCACGTGGCCAGAACCAGAAACCGTCTCGACCGAACCCCCTTCCAGACCGCGGTCACCGACCT
GAGCCATGACGGCCGCGGGGTCGCCCGCCGCGACGGCGAGGGCGGCAAGGTCACCTTCATCAGCGGCGCCTTGCCGGGCG
AGTTGGTGCGCGCCGAACCTACCGCCCGTAGCCGGCATTTCGATGAGGCCAAGACCGTGGGGGTGCTGGAGGCATCGCCG
CAGCGGGTCACCCCGCGCTGCCCGCACTTTGGCGTCTGCGCAGGCTGTGTGTTGCAGCATCTGGAGGAATCGCAGCAGAT
CGTGGCCAAACAGCGCGTACTGATGGATAACCTGGAGCGTATCGGTCATGTCACTCCGCAAGCCGTGCTGCCTGCGCTGA
CCGGCGACAACTGGGGGTACCGGCGCAAGGGCAGGTTCTCGGTGCGTCGTGTCGAGAAGAAGGACAAGACCTTGGTCGGC
TTTCGTGAGCTCGACCCGCGGTTCGTGGCCGATCTGTCCGTTTGCTACACGGTGATTCCGCAGATCGGCGAGAAGATCCC
CTTGCTTGCCGCGCTGATCGAAGGCATGGATGGCAAGCGCGACATTCCGCAGATCGAATTCATCGCCGGCGACGATGCGG
TGGCCTTGACCATTCGGCATATGCAGCCGCTCAGCGAACGCGACCGCCAGGCCTGGATCACATTTGCCCAGGAGCACGGC
TTTGCGATTTTTCTGCAGCCCGGCGGGGTGGACAGCGTGCAGCCGCTGTGGCCGCAGGAAGTGCCGCTATCGTTCCGCTT
GCCGCAGTGGGATGTGGAACTAGCGTTCCGGCCGCTGGACTTTATTCAGGTCAACGCGTCGCTCAATCAGAAGATGATCG
CGCATGCAGTCGCGCTGTTGGAGGCCAAGCCCGACGACCGCGTGCTGGACCTGTTCTGCGGCCTGGGCAACTTCACCCTG
CCGCTGGCGCGCGTAGTGCGCGAAGTGGTGGGTGTGGAAGGCGACGCCGGACTTGTGGCACGGGCGAAGGAGAACGCCCA
GCGCAATGGCCTGGACAATGCGCAGTTCTACGCCGCCGACCTGACCCAGGATCAGCGCAGCGCACCCTGGATGCGCCAGG
GCTTCGACAAGCTGCTGCTGGACCCGCCGCGCTCGGGCGCCCTGGAAGTGCTGCAGCAGTTGCCGTTGAAGACGTTCCAG
CGCATCGTCTATGTGAGCTGCCATCCTGGCTCGCTGGCGCGCGACGCCGGTTATCTGGTCAACGAGCAGGGCTTCACGCT
GGTCTCGGCGGGCGCGATGGACATGTTCCCGCACACCGCGCACGTGGAAAGCATTGCGGTGTTTGAGAGGCGGTGA

Upstream 100 bases:

>100_bases
CGCGCGCAGCGGGAGTTTGCCTGTGTTTGTGGGGCGCGACAATTGTTGCGCTGACCGGCGCGGCCTCCGTCGATCGCATC
TGCGACAATACGCACCCTTT

Downstream 100 bases:

>100_bases
ATCGGGAGTAGGCAGTAGGGAATCGTAACGGCGAACGGCTTATGCTTTTGCCATTCCCGATTCCCGATTCCCGATTCCCG
ATTCCCATGCCCACAGAAAT

Product: 23S rRNA 5-methyluridine methyltransferase

Products: NA

Alternate protein names: 23S rRNA(M-5-U1939)-methyltransferase [H]

Number of amino acids: Translated: 451; Mature: 450

Protein sequence:

>451_residues
MPAACRHVARTRNRLDRTPFQTAVTDLSHDGRGVARRDGEGGKVTFISGALPGELVRAEPTARSRHFDEAKTVGVLEASP
QRVTPRCPHFGVCAGCVLQHLEESQQIVAKQRVLMDNLERIGHVTPQAVLPALTGDNWGYRRKGRFSVRRVEKKDKTLVG
FRELDPRFVADLSVCYTVIPQIGEKIPLLAALIEGMDGKRDIPQIEFIAGDDAVALTIRHMQPLSERDRQAWITFAQEHG
FAIFLQPGGVDSVQPLWPQEVPLSFRLPQWDVELAFRPLDFIQVNASLNQKMIAHAVALLEAKPDDRVLDLFCGLGNFTL
PLARVVREVVGVEGDAGLVARAKENAQRNGLDNAQFYAADLTQDQRSAPWMRQGFDKLLLDPPRSGALEVLQQLPLKTFQ
RIVYVSCHPGSLARDAGYLVNEQGFTLVSAGAMDMFPHTAHVESIAVFERR

Sequences:

>Translated_451_residues
MPAACRHVARTRNRLDRTPFQTAVTDLSHDGRGVARRDGEGGKVTFISGALPGELVRAEPTARSRHFDEAKTVGVLEASP
QRVTPRCPHFGVCAGCVLQHLEESQQIVAKQRVLMDNLERIGHVTPQAVLPALTGDNWGYRRKGRFSVRRVEKKDKTLVG
FRELDPRFVADLSVCYTVIPQIGEKIPLLAALIEGMDGKRDIPQIEFIAGDDAVALTIRHMQPLSERDRQAWITFAQEHG
FAIFLQPGGVDSVQPLWPQEVPLSFRLPQWDVELAFRPLDFIQVNASLNQKMIAHAVALLEAKPDDRVLDLFCGLGNFTL
PLARVVREVVGVEGDAGLVARAKENAQRNGLDNAQFYAADLTQDQRSAPWMRQGFDKLLLDPPRSGALEVLQQLPLKTFQ
RIVYVSCHPGSLARDAGYLVNEQGFTLVSAGAMDMFPHTAHVESIAVFERR
>Mature_450_residues
PAACRHVARTRNRLDRTPFQTAVTDLSHDGRGVARRDGEGGKVTFISGALPGELVRAEPTARSRHFDEAKTVGVLEASPQ
RVTPRCPHFGVCAGCVLQHLEESQQIVAKQRVLMDNLERIGHVTPQAVLPALTGDNWGYRRKGRFSVRRVEKKDKTLVGF
RELDPRFVADLSVCYTVIPQIGEKIPLLAALIEGMDGKRDIPQIEFIAGDDAVALTIRHMQPLSERDRQAWITFAQEHGF
AIFLQPGGVDSVQPLWPQEVPLSFRLPQWDVELAFRPLDFIQVNASLNQKMIAHAVALLEAKPDDRVLDLFCGLGNFTLP
LARVVREVVGVEGDAGLVARAKENAQRNGLDNAQFYAADLTQDQRSAPWMRQGFDKLLLDPPRSGALEVLQQLPLKTFQR
IVYVSCHPGSLARDAGYLVNEQGFTLVSAGAMDMFPHTAHVESIAVFERR

Specific function: Catalyzes the formation of 5-methyl-uridine at position 1939 (M-5-U1939) in 23S rRNA [H]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI34222389, Length=193, Percent_Identity=35.2331606217617, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI51173878, Length=193, Percent_Identity=35.2331606217617, Blast_Score=81, Evalue=2e-15,
Organism=Escherichia coli, GI1789148, Length=448, Percent_Identity=39.0625, Blast_Score=314, Evalue=8e-87,
Organism=Escherichia coli, GI1787083, Length=185, Percent_Identity=32.972972972973, Blast_Score=85, Evalue=8e-18,
Organism=Escherichia coli, GI1790403, Length=192, Percent_Identity=32.2916666666667, Blast_Score=85, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI71997438, Length=169, Percent_Identity=29.585798816568, Blast_Score=69, Evalue=6e-12,
Organism=Drosophila melanogaster, GI24666579, Length=196, Percent_Identity=34.1836734693878, Blast_Score=93, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016027
- InterPro:   IPR001566
- InterPro:   IPR002792
- InterPro:   IPR010280 [H]

Pfam domain/function: PF05958 tRNA_U5-meth_tr [H]

EC number: =2.1.1.190 [H]

Molecular weight: Translated: 50081; Mature: 49950

Theoretical pI: Translated: 7.60; Mature: 7.60

Prosite motif: PS50926 TRAM ; PS01231 TRMA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAACRHVARTRNRLDRTPFQTAVTDLSHDGRGVARRDGEGGKVTFISGALPGELVRAEP
CCHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCCCEECCC
TARSRHFDEAKTVGVLEASPQRVTPRCPHFGVCAGCVLQHLEESQQIVAKQRVLMDNLER
CHHHCCCCHHHHEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IGHVTPQAVLPALTGDNWGYRRKGRFSVRRVEKKDKTLVGFRELDPRFVADLSVCYTVIP
HCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCEEECHHHCCCHHHHHHHHHHHHHH
QIGEKIPLLAALIEGMDGKRDIPQIEFIAGDDAVALTIRHMQPLSERDRQAWITFAQEHG
HHCCHHHHHHHHHCCCCCCCCCCEEEEEECCCEEEEEEHHHCCHHHHHHHHHEEEEECCC
FAIFLQPGGVDSVQPLWPQEVPLSFRLPQWDVELAFRPLDFIQVNASLNQKMIAHAVALL
EEEEECCCCCCCCCCCCCCCCCCEEECCCCCEEEEECCCEEEEECCCHHHHHHHHHHHHH
EAKPDDRVLDLFCGLGNFTLPLARVVREVVGVEGDAGLVARAKENAQRNGLDNAQFYAAD
HCCCCCHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHCCCCCCEEEEEC
LTQDQRSAPWMRQGFDKLLLDPPRSGALEVLQQLPLKTFQRIVYVSCHPGSLARDAGYLV
CCCCCCCCCHHHHCCHHHHCCCCCCCHHHHHHHCCHHHHHHEEEEEECCCCCHHCCCCEE
NEQGFTLVSAGAMDMFPHTAHVESIAVFERR
CCCCCEEEECCCHHHCCCHHHHHHHHHCCCC
>Mature Secondary Structure 
PAACRHVARTRNRLDRTPFQTAVTDLSHDGRGVARRDGEGGKVTFISGALPGELVRAEP
CHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCCCEECCC
TARSRHFDEAKTVGVLEASPQRVTPRCPHFGVCAGCVLQHLEESQQIVAKQRVLMDNLER
CHHHCCCCHHHHEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IGHVTPQAVLPALTGDNWGYRRKGRFSVRRVEKKDKTLVGFRELDPRFVADLSVCYTVIP
HCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCEEECHHHCCCHHHHHHHHHHHHHH
QIGEKIPLLAALIEGMDGKRDIPQIEFIAGDDAVALTIRHMQPLSERDRQAWITFAQEHG
HHCCHHHHHHHHHCCCCCCCCCCEEEEEECCCEEEEEEHHHCCHHHHHHHHHEEEEECCC
FAIFLQPGGVDSVQPLWPQEVPLSFRLPQWDVELAFRPLDFIQVNASLNQKMIAHAVALL
EEEEECCCCCCCCCCCCCCCCCCEEECCCCCEEEEECCCEEEEECCCHHHHHHHHHHHHH
EAKPDDRVLDLFCGLGNFTLPLARVVREVVGVEGDAGLVARAKENAQRNGLDNAQFYAAD
HCCCCCHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHCCCCCCEEEEEC
LTQDQRSAPWMRQGFDKLLLDPPRSGALEVLQQLPLKTFQRIVYVSCHPGSLARDAGYLV
CCCCCCCCCHHHHCCHHHHCCCCCCCHHHHHHHCCHHHHHHEEEEEECCCCCHHCCCCEE
NEQGFTLVSAGAMDMFPHTAHVESIAVFERR
CCCCCEEEECCCHHHCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]