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The map label for this gene is colS [H]

Identifier: 21241976

GI number: 21241976

Start: 1401768

End: 1403057

Strand: Direct

Name: colS [H]

Synonym: XAC1222

Alternate gene names: 21241976

Gene position: 1401768-1403057 (Clockwise)

Preceding gene: 21241975

Following gene: 21241982

Centisome position: 27.08

GC content: 65.97

Gene sequence:

>1290_bases
ATGTCTAAACAGCGCCCGTTGGGTCGCCGCGTGCTGGTGTGGTTGTTCGGCTACACGCTGTTGATGACCCTGGCGGTGTT
CGGCACCGCGCAGTACCTGCACGAACGTGCCGAACACGGCGTGTGGCGCTCGCTGCTGAATTCCGAACTGGACAGCATCC
TCGAGCGCAGCCTGCAGGATCCGGATTACCGCTGGCAGGACTCGGACACCTTGCGGCTGTACCGGGTCGACGACGTCGCG
CGCACACCGCCGGTCTTGCGCACCCTGCATCCCGGCTTGCACGACGGTCTGGAGATCGCCGGCCGCCAGAGTGCCGTGAT
GGTGCGCGACACCGCACATGCCGGCCGCCTGGCCCTGGTGCTGGACATCACCGATTTCGAAGCGTTGGAAAAATTTCTGA
CCCGCTGGATGCTGGCCGCTGGCATCGCGCTGATCGGCATCACCTTGCTGATGGGCACATATGCGATGGCGCGGCTGGTG
CGCCCGCTGGTGGAACTTGCGCGCGACATCGGCGCGTTGCGTCCAGAGCAGCGCGCGCAGCAGATTGCGGTAGGGCCACA
GGGCAGCGCCGAGCTGTACGTGATTGCCGATGCGCTCAACGATTATCTGGAGCGCAATAGCCAGTTCGTCGACCGTGAGC
GGGCTTTCATCGACAGCGCCAGCCATGAATTACGCACGCCCATCGCGGTGATCGGCAATGCGGTGGAGCTGGCGCTGGAC
CAACCCGGCACGCCGCCGGCGGTGCGCCACCAGCTGCAGCGCATCGCGCAGACCAGCACCTCGGTCGAGCAGTTGATCAC
CTTGCTGCTGGTGCTGGCCAAAGATCCTGGCCGGCTGGTGCGCAGCAGCGATGCGTTGCGGCTGGATCAGTTGCTGCCGG
ACATCGTGGCCGACCACACGCACCTGTGCGCCGACAAGGATCTGCAACTGGTCGTGGCCCCGCTGCCGCCATGCAGCGTG
GTCGCCCCGGTCGCCATCGTGCAGACGGCCATCGGCAACCTGCTGCGCAACGCCATCGAAAACAGCGACCGCGGCATCAT
TCGCATCTCGCTGTCGGCGCCCGGCGTGGTCTGCATTGCCGACCCCGGCCATGGCATGACGCCCGAAGAGATCAGCGCCA
TCTACACGCGCCTGGCGCGCGGCAACGTCCGCCAGGGGAGCGGTATCGGGCTGGAACTGATCGGACGCTTGTGCGAACAC
CTAGGCTGGCATCTGCAGCTGGATTCCAATGCCGGGCAGGGCACGGTGGCCACGCTGGATCTCTCCGGCGCCACGGCAAC
GGAATCCTGA

Upstream 100 bases:

>100_bases
GGCCCGTTCGCGCAAAAATTGATCCATACCTTGCCGCGGCTCGGCTACCGGCTGGCGCAGCTGGATGCGGCCGATGCGCC
TGGCGCTGGCGACTGCAGCG

Downstream 100 bases:

>100_bases
GGGGATCACGTAACCGACTGCACTCACGCCAGGCGGTGCGTCCGCTATTCGATACTGCAGGTGCCACACGCCCGCTGCGG
GTCCTGCGAAGGCGCCACAC

Product: two-component system sensor protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 429; Mature: 428

Protein sequence:

>429_residues
MSKQRPLGRRVLVWLFGYTLLMTLAVFGTAQYLHERAEHGVWRSLLNSELDSILERSLQDPDYRWQDSDTLRLYRVDDVA
RTPPVLRTLHPGLHDGLEIAGRQSAVMVRDTAHAGRLALVLDITDFEALEKFLTRWMLAAGIALIGITLLMGTYAMARLV
RPLVELARDIGALRPEQRAQQIAVGPQGSAELYVIADALNDYLERNSQFVDRERAFIDSASHELRTPIAVIGNAVELALD
QPGTPPAVRHQLQRIAQTSTSVEQLITLLLVLAKDPGRLVRSSDALRLDQLLPDIVADHTHLCADKDLQLVVAPLPPCSV
VAPVAIVQTAIGNLLRNAIENSDRGIIRISLSAPGVVCIADPGHGMTPEEISAIYTRLARGNVRQGSGIGLELIGRLCEH
LGWHLQLDSNAGQGTVATLDLSGATATES

Sequences:

>Translated_429_residues
MSKQRPLGRRVLVWLFGYTLLMTLAVFGTAQYLHERAEHGVWRSLLNSELDSILERSLQDPDYRWQDSDTLRLYRVDDVA
RTPPVLRTLHPGLHDGLEIAGRQSAVMVRDTAHAGRLALVLDITDFEALEKFLTRWMLAAGIALIGITLLMGTYAMARLV
RPLVELARDIGALRPEQRAQQIAVGPQGSAELYVIADALNDYLERNSQFVDRERAFIDSASHELRTPIAVIGNAVELALD
QPGTPPAVRHQLQRIAQTSTSVEQLITLLLVLAKDPGRLVRSSDALRLDQLLPDIVADHTHLCADKDLQLVVAPLPPCSV
VAPVAIVQTAIGNLLRNAIENSDRGIIRISLSAPGVVCIADPGHGMTPEEISAIYTRLARGNVRQGSGIGLELIGRLCEH
LGWHLQLDSNAGQGTVATLDLSGATATES
>Mature_428_residues
SKQRPLGRRVLVWLFGYTLLMTLAVFGTAQYLHERAEHGVWRSLLNSELDSILERSLQDPDYRWQDSDTLRLYRVDDVAR
TPPVLRTLHPGLHDGLEIAGRQSAVMVRDTAHAGRLALVLDITDFEALEKFLTRWMLAAGIALIGITLLMGTYAMARLVR
PLVELARDIGALRPEQRAQQIAVGPQGSAELYVIADALNDYLERNSQFVDRERAFIDSASHELRTPIAVIGNAVELALDQ
PGTPPAVRHQLQRIAQTSTSVEQLITLLLVLAKDPGRLVRSSDALRLDQLLPDIVADHTHLCADKDLQLVVAPLPPCSVV
APVAIVQTAIGNLLRNAIENSDRGIIRISLSAPGVVCIADPGHGMTPEEISAIYTRLARGNVRQGSGIGLELIGRLCEHL
GWHLQLDSNAGQGTVATLDLSGATATES

Specific function: Member of a two-component regulatory system qseB/qseC. May activate qseB by phosphorylation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1790551, Length=290, Percent_Identity=26.2068965517241, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1789403, Length=288, Percent_Identity=28.125, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI1790861, Length=297, Percent_Identity=25.5892255892256, Blast_Score=66, Evalue=5e-12,
Organism=Escherichia coli, GI1790346, Length=243, Percent_Identity=24.2798353909465, Blast_Score=62, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 46853; Mature: 46721

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKQRPLGRRVLVWLFGYTLLMTLAVFGTAQYLHERAEHGVWRSLLNSELDSILERSLQD
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PDYRWQDSDTLRLYRVDDVARTPPVLRTLHPGLHDGLEIAGRQSAVMVRDTAHAGRLALV
CCCCCCCCCCEEEEEECCHHCCCHHHHHHCCCHHHHHHHCCCCCEEEEEECCCCCCEEEE
LDITDFEALEKFLTRWMLAAGIALIGITLLMGTYAMARLVRPLVELARDIGALRPEQRAQ
EECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
QIAVGPQGSAELYVIADALNDYLERNSQFVDRERAFIDSASHELRTPIAVIGNAVELALD
HEECCCCCCCEEEEEHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCHHHHHCCHHHEEEC
QPGTPPAVRHQLQRIAQTSTSVEQLITLLLVLAKDPGRLVRSSDALRLDQLLPDIVADHT
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCHHHHHHHHHHHHHCCH
HLCADKDLQLVVAPLPPCSVVAPVAIVQTAIGNLLRNAIENSDRGIIRISLSAPGVVCIA
HHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEE
DPGHGMTPEEISAIYTRLARGNVRQGSGIGLELIGRLCEHLGWHLQLDSNAGQGTVATLD
CCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCEEEECCCCCCCEEEEEE
LSGATATES
ECCCCCCCC
>Mature Secondary Structure 
SKQRPLGRRVLVWLFGYTLLMTLAVFGTAQYLHERAEHGVWRSLLNSELDSILERSLQD
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PDYRWQDSDTLRLYRVDDVARTPPVLRTLHPGLHDGLEIAGRQSAVMVRDTAHAGRLALV
CCCCCCCCCCEEEEEECCHHCCCHHHHHHCCCHHHHHHHCCCCCEEEEEECCCCCCEEEE
LDITDFEALEKFLTRWMLAAGIALIGITLLMGTYAMARLVRPLVELARDIGALRPEQRAQ
EECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
QIAVGPQGSAELYVIADALNDYLERNSQFVDRERAFIDSASHELRTPIAVIGNAVELALD
HEECCCCCCCEEEEEHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCHHHHHCCHHHEEEC
QPGTPPAVRHQLQRIAQTSTSVEQLITLLLVLAKDPGRLVRSSDALRLDQLLPDIVADHT
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCHHHHHHHHHHHHHCCH
HLCADKDLQLVVAPLPPCSVVAPVAIVQTAIGNLLRNAIENSDRGIIRISLSAPGVVCIA
HHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEE
DPGHGMTPEEISAIYTRLARGNVRQGSGIGLELIGRLCEHLGWHLQLDSNAGQGTVATLD
CCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCEEEECCCCCCCEEEEEE
LSGATATES
ECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023 [H]