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The map label for this gene is prpB [H]

Identifier: 21241891

GI number: 21241891

Start: 1286872

End: 1287768

Strand: Direct

Name: prpB [H]

Synonym: XAC1137

Alternate gene names: 21241891

Gene position: 1286872-1287768 (Clockwise)

Preceding gene: 21241889

Following gene: 21241892

Centisome position: 24.86

GC content: 64.66

Gene sequence:

>897_bases
ATGACGTTCTCGTCCGCTCCCTCTGCCGGCACGCGGTTTCGCGCCGCACTGGCTGCCGAATCGCCGCTGCAGGTAATCGG
CGCGATCAATGCCAACCATGCGCTGCTGGCGCAGCGCGCAGGCTATCAGGCGATTTATCTGTCAGGCGGCGGCGTGGCGG
CCGGGTCGCTGGGGTTGCCGGATCTGGGCATCAACACGCTGGAAGACGTGCTGATCGACGTGCGCCGTATCACCGACGTG
TGCGAGCTGCCGCTGTTGGTGGATGTGGACACCGGTTTTGGCCCGAGCGCATTCAACATCGAGCGCACCATCAAGTCGCT
GATCAAGGCGGGCGCGGGCGGTTGCCATATCGAAGACCAGGTTGGTGCCAAGCGCTGCGGTCACCGGCCGGGCAAGGAAA
TCGTGAGCCAGGGCGAGATGGCGGATCGGGTCAAGGCCGCAGCCGATGCCAAGACCGATGCGGCGTTCTTCCTGATTGCG
CGTACCGACGCAATCCAGATGGAAGGCGTGGAGGCGGCGATCGAACGCGCCATCGCCTGCGTGGAAGCGGGTGCGGACGG
CATCTTCGCCGAAGCCGCCTACGACCTGGATACCTACAAGCGTTTTGTCGATGCAGTGCGCGTGCCGGTGCTGGCCAACA
TCACCGAGTTCGGCAAGACCCCGCTGTTTACGCGCGACCAGCTGGCGCAAGCCGGCGTGGCGATCCAGCTGTTTCCGCTG
TCGGCATTTCGCGCCGCCAACAAGGCGGCTGAGGCGGTTTATACGGCGATTCGGCGCGACGGTCATCAGCAGGGCGTGCT
GGACAGCATGCAGACGCGCGAAGAGCTGTACGAACGCATCGGCTATCACGAGTACGAGCAGCGGCTGGACGCGTTGTTTG
CGCGCGAAGGCGCATGA

Upstream 100 bases:

>100_bases
CAATTTTGCAACAATCGATGCCCTGGGTGGTATGAAAATCAACGAGTTACGCTTGGCATGGTGTTTGCGGCTGCACGGTC
ATCCCATCCAAGGAACCGCC

Downstream 100 bases:

>100_bases
TGACGCGTTGCTGATCATCTTCATTGCCACCTCGCACGCCATACCGCGACACCACACAGTTTGGGAGCTCATCGCATGAA
CGACACTGCCACTCCTGGTT

Product: 2-methylisocitrate lyase

Products: NA

Alternate protein names: 2-methylisocitrate lyase [H]

Number of amino acids: Translated: 298; Mature: 297

Protein sequence:

>298_residues
MTFSSAPSAGTRFRAALAAESPLQVIGAINANHALLAQRAGYQAIYLSGGGVAAGSLGLPDLGINTLEDVLIDVRRITDV
CELPLLVDVDTGFGPSAFNIERTIKSLIKAGAGGCHIEDQVGAKRCGHRPGKEIVSQGEMADRVKAAADAKTDAAFFLIA
RTDAIQMEGVEAAIERAIACVEAGADGIFAEAAYDLDTYKRFVDAVRVPVLANITEFGKTPLFTRDQLAQAGVAIQLFPL
SAFRAANKAAEAVYTAIRRDGHQQGVLDSMQTREELYERIGYHEYEQRLDALFAREGA

Sequences:

>Translated_298_residues
MTFSSAPSAGTRFRAALAAESPLQVIGAINANHALLAQRAGYQAIYLSGGGVAAGSLGLPDLGINTLEDVLIDVRRITDV
CELPLLVDVDTGFGPSAFNIERTIKSLIKAGAGGCHIEDQVGAKRCGHRPGKEIVSQGEMADRVKAAADAKTDAAFFLIA
RTDAIQMEGVEAAIERAIACVEAGADGIFAEAAYDLDTYKRFVDAVRVPVLANITEFGKTPLFTRDQLAQAGVAIQLFPL
SAFRAANKAAEAVYTAIRRDGHQQGVLDSMQTREELYERIGYHEYEQRLDALFAREGA
>Mature_297_residues
TFSSAPSAGTRFRAALAAESPLQVIGAINANHALLAQRAGYQAIYLSGGGVAAGSLGLPDLGINTLEDVLIDVRRITDVC
ELPLLVDVDTGFGPSAFNIERTIKSLIKAGAGGCHIEDQVGAKRCGHRPGKEIVSQGEMADRVKAAADAKTDAAFFLIAR
TDAIQMEGVEAAIERAIACVEAGADGIFAEAAYDLDTYKRFVDAVRVPVLANITEFGKTPLFTRDQLAQAGVAIQLFPLS
AFRAANKAAEAVYTAIRRDGHQQGVLDSMQTREELYERIGYHEYEQRLDALFAREGA

Specific function: Catalyzes the formation of pyruvate and succinate from 2-methylisocitrate [H]

COG id: COG2513

COG function: function code G; PEP phosphonomutase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate lyase/PEP mutase superfamily. Methylisocitrate lyase family [H]

Homologues:

Organism=Escherichia coli, GI1786525, Length=291, Percent_Identity=69.4158075601375, Blast_Score=422, Evalue=1e-119,
Organism=Escherichia coli, GI1790445, Length=295, Percent_Identity=26.4406779661017, Blast_Score=72, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI71982926, Length=190, Percent_Identity=31.0526315789474, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17561814, Length=190, Percent_Identity=31.0526315789474, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000918
- InterPro:   IPR018523
- InterPro:   IPR012695
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00463 ICL [H]

EC number: =4.1.3.30 [H]

Molecular weight: Translated: 31801; Mature: 31670

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS00161 ISOCITRATE_LYASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFSSAPSAGTRFRAALAAESPLQVIGAINANHALLAQRAGYQAIYLSGGGVAAGSLGLP
CCCCCCCCCCHHHHHHHHCCCCHHHHCCCCCCHHHHHHHCCCEEEEEECCCEEECCCCCC
DLGINTLEDVLIDVRRITDVCELPLLVDVDTGFGPSAFNIERTIKSLIKAGAGGCHIEDQ
CCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCHH
VGAKRCGHRPGKEIVSQGEMADRVKAAADAKTDAAFFLIARTDAIQMEGVEAAIERAIAC
HHHHHHCCCCCHHHHHCCHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHH
VEAGADGIFAEAAYDLDTYKRFVDAVRVPVLANITEFGKTPLFTRDQLAQAGVAIQLFPL
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCEEEEECH
SAFRAANKAAEAVYTAIRRDGHQQGVLDSMQTREELYERIGYHEYEQRLDALFAREGA
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TFSSAPSAGTRFRAALAAESPLQVIGAINANHALLAQRAGYQAIYLSGGGVAAGSLGLP
CCCCCCCCCHHHHHHHHCCCCHHHHCCCCCCHHHHHHHCCCEEEEEECCCEEECCCCCC
DLGINTLEDVLIDVRRITDVCELPLLVDVDTGFGPSAFNIERTIKSLIKAGAGGCHIEDQ
CCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCHH
VGAKRCGHRPGKEIVSQGEMADRVKAAADAKTDAAFFLIARTDAIQMEGVEAAIERAIAC
HHHHHHCCCCCHHHHHCCHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHH
VEAGADGIFAEAAYDLDTYKRFVDAVRVPVLANITEFGKTPLFTRDQLAQAGVAIQLFPL
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCEEEEECH
SAFRAANKAAEAVYTAIRRDGHQQGVLDSMQTREELYERIGYHEYEQRLDALFAREGA
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503; 9325432 [H]