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The map label for this gene is tuf [H]

Identifier: 21241723

GI number: 21241723

Start: 1116063

End: 1117253

Strand: Direct

Name: tuf [H]

Synonym: XAC0957

Alternate gene names: 21241723

Gene position: 1116063-1117253 (Clockwise)

Preceding gene: 21241722

Following gene: 21241724

Centisome position: 21.56

GC content: 63.48

Gene sequence:

>1191_bases
ATGGCAAAAGCTAAATTCGAGCGCACCAAGCCGCACGTGAACGTCGGCACCATCGGTCACGTTGACCATGGCAAGACCAC
GTTGACCGCTGCGCTGACCAAGATCGGTGCCGAGCGTTTCGGTGGTGAGTTCAAGGCGTACGACGCGATCGACGCGGCGC
CGGAAGAGAAGGCGCGCGGCATCACGATTTCGACCGCACACGTCGAATACGAATCCCCGACCCGCCACTACGCGCACGTC
GACTGCCCCGGCCACGCCGACTACGTCAAGAACATGATCACCGGTGCGGCGCAGATGGACGGCGCGATCCTGGTGTGCTC
GGCCGCCGACGGCCCGATGCCGCAGACCCGTGAGCACATCCTGCTCTCGCGTCAGGTCGGTGTGCCGCACATCGTGGTGT
TCCTGAACAAGGCCGACATGGTCGACGACGCCGAGCTGCTCGAGCTGGTCGAAATGGAGGTGCGCGAGCTGCTGAGCAAG
TACGACTTCCCGGGCGACGACACCCCGATCATCCACGGTTCGGCGCGTCTGGCGCTGGACGGCGATCAGAGCGACATCGG
TGTGCCGGCGATCCTGAAGCTGGTCGAAGCGCTGGATTCGTTCATTCCCGAGCCGACCCGCGACGTCGACCGTCCGTTCC
TGATGCCGGTCGAAGACGTGTTCTCGATCTCCGGCCGCGGCACCGTGGTGACCGGTCGTATCGAGCGCGGCATCATCAAG
GTGGGCGACGAAATCGAAATCGTCGGTATCCGCGATACGCAGAAGACCACGGTCACCGGCGTGGAAATGTTCCGCAAGCT
GCTGGACCAGGGCCAGGCAGGCGACAACGCCGGTCTGCTGCTGCGCGGCACCAAGCGTGACGACGTCGAGCGTGGGCAGG
TGCTGTGCAAGCCGGGTTCGATCAAGCCGCACACCGAGTTCGAAGCCGAAGTCTACGTGCTGTCCAAGGATGAGGGTGGC
CGTCATACCCCGTTCTTCAAGGGCTACCGTCCGCAGTTCTACTTCCGCACCACCGACATCACTGGCGCTTGCCAGTTGCC
GGAAGGCGTGGAAATGGTGATGCCGGGCGACAACGTGAAGATGGTCGTGACCCTGATCAACCCGGTCGCGATGGACGAAG
GTCTGCGCTTCGCCATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTGGTTGCCAAGATCATCAAGTAA

Upstream 100 bases:

>100_bases
AGCACCTCCTTGGTAAGGAGGAGGTCGAAGGTTCGATTCCTTTCGTGAGCACCATTACTCAATCGTCTTCAACTCCAAAC
GATTACCGAGACACGCACCC

Downstream 100 bases:

>100_bases
GTGCCTGTATCCAGCTGCCTGTTGGTGGCTGGATTCAAACGAATGGCGGGCCGGCTGAACCGCGGTCCGCCTTCGTTGTC
TTGGGGTTTGCGGTAAACGA

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu 1 [H]

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MAKAKFERTKPHVNVGTIGHVDHGKTTLTAALTKIGAERFGGEFKAYDAIDAAPEEKARGITISTAHVEYESPTRHYAHV
DCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHILLSRQVGVPHIVVFLNKADMVDDAELLELVEMEVRELLSK
YDFPGDDTPIIHGSARLALDGDQSDIGVPAILKLVEALDSFIPEPTRDVDRPFLMPVEDVFSISGRGTVVTGRIERGIIK
VGDEIEIVGIRDTQKTTVTGVEMFRKLLDQGQAGDNAGLLLRGTKRDDVERGQVLCKPGSIKPHTEFEAEVYVLSKDEGG
RHTPFFKGYRPQFYFRTTDITGACQLPEGVEMVMPGDNVKMVVTLINPVAMDEGLRFAIREGGRTVGAGVVAKIIK

Sequences:

>Translated_396_residues
MAKAKFERTKPHVNVGTIGHVDHGKTTLTAALTKIGAERFGGEFKAYDAIDAAPEEKARGITISTAHVEYESPTRHYAHV
DCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHILLSRQVGVPHIVVFLNKADMVDDAELLELVEMEVRELLSK
YDFPGDDTPIIHGSARLALDGDQSDIGVPAILKLVEALDSFIPEPTRDVDRPFLMPVEDVFSISGRGTVVTGRIERGIIK
VGDEIEIVGIRDTQKTTVTGVEMFRKLLDQGQAGDNAGLLLRGTKRDDVERGQVLCKPGSIKPHTEFEAEVYVLSKDEGG
RHTPFFKGYRPQFYFRTTDITGACQLPEGVEMVMPGDNVKMVVTLINPVAMDEGLRFAIREGGRTVGAGVVAKIIK
>Mature_395_residues
AKAKFERTKPHVNVGTIGHVDHGKTTLTAALTKIGAERFGGEFKAYDAIDAAPEEKARGITISTAHVEYESPTRHYAHVD
CPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHILLSRQVGVPHIVVFLNKADMVDDAELLELVEMEVRELLSKY
DFPGDDTPIIHGSARLALDGDQSDIGVPAILKLVEALDSFIPEPTRDVDRPFLMPVEDVFSISGRGTVVTGRIERGIIKV
GDEIEIVGIRDTQKTTVTGVEMFRKLLDQGQAGDNAGLLLRGTKRDDVERGQVLCKPGSIKPHTEFEAEVYVLSKDEGGR
HTPFFKGYRPQFYFRTTDITGACQLPEGVEMVMPGDNVKMVVTLINPVAMDEGLRFAIREGGRTVGAGVVAKIIK

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=394, Percent_Identity=57.3604060913706, Blast_Score=486, Evalue=1e-137,
Organism=Homo sapiens, GI4503475, Length=451, Percent_Identity=29.490022172949, Blast_Score=161, Evalue=1e-39,
Organism=Homo sapiens, GI223555963, Length=443, Percent_Identity=30.2483069977427, Blast_Score=161, Evalue=1e-39,
Organism=Homo sapiens, GI4503471, Length=457, Percent_Identity=29.3216630196937, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI5729864, Length=443, Percent_Identity=30.2483069977427, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI194018522, Length=338, Percent_Identity=31.6568047337278, Blast_Score=127, Evalue=1e-29,
Organism=Homo sapiens, GI194097354, Length=338, Percent_Identity=31.6568047337278, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI194018520, Length=338, Percent_Identity=31.6568047337278, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI54607086, Length=345, Percent_Identity=29.5652173913043, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI46094014, Length=324, Percent_Identity=31.7901234567901, Blast_Score=125, Evalue=8e-29,
Organism=Homo sapiens, GI4503507, Length=361, Percent_Identity=29.0858725761773, Blast_Score=96, Evalue=6e-20,
Organism=Homo sapiens, GI82546879, Length=432, Percent_Identity=25, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI1790412, Length=395, Percent_Identity=78.9873417721519, Blast_Score=627, Evalue=0.0,
Organism=Escherichia coli, GI1789737, Length=395, Percent_Identity=78.9873417721519, Blast_Score=627, Evalue=0.0,
Organism=Escherichia coli, GI2367247, Length=378, Percent_Identity=29.1005291005291, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI48994988, Length=237, Percent_Identity=31.2236286919831, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1789108, Length=434, Percent_Identity=28.3410138248848, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1788922, Length=331, Percent_Identity=25.9818731117825, Blast_Score=62, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI17556456, Length=399, Percent_Identity=53.1328320802005, Blast_Score=403, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI25141371, Length=387, Percent_Identity=44.4444444444444, Blast_Score=340, Evalue=7e-94,
Organism=Caenorhabditis elegans, GI17552884, Length=453, Percent_Identity=31.5673289183223, Blast_Score=162, Evalue=3e-40,
Organism=Caenorhabditis elegans, GI17569207, Length=453, Percent_Identity=31.5673289183223, Blast_Score=162, Evalue=3e-40,
Organism=Caenorhabditis elegans, GI32566303, Length=440, Percent_Identity=31.1363636363636, Blast_Score=139, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI32566629, Length=349, Percent_Identity=30.3724928366762, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17506081, Length=299, Percent_Identity=30.1003344481605, Blast_Score=125, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI115532067, Length=445, Percent_Identity=26.7415730337079, Blast_Score=122, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI115532065, Length=445, Percent_Identity=26.7415730337079, Blast_Score=121, Evalue=8e-28,
Organism=Caenorhabditis elegans, GI17509919, Length=438, Percent_Identity=25.5707762557078, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI32566301, Length=147, Percent_Identity=38.0952380952381, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17557151, Length=237, Percent_Identity=27.0042194092827, Blast_Score=79, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6324761, Length=398, Percent_Identity=65.3266331658292, Blast_Score=538, Evalue=1e-154,
Organism=Saccharomyces cerevisiae, GI6325337, Length=453, Percent_Identity=30.9050772626932, Blast_Score=164, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6319594, Length=453, Percent_Identity=30.9050772626932, Blast_Score=164, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6320377, Length=341, Percent_Identity=29.9120234604106, Blast_Score=93, Evalue=9e-20,
Organism=Saccharomyces cerevisiae, GI6320863, Length=269, Percent_Identity=28.6245353159851, Blast_Score=91, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6322937, Length=455, Percent_Identity=23.2967032967033, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6323320, Length=238, Percent_Identity=27.3109243697479, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI281363316, Length=393, Percent_Identity=59.0330788804071, Blast_Score=495, Evalue=1e-140,
Organism=Drosophila melanogaster, GI17864358, Length=393, Percent_Identity=59.0330788804071, Blast_Score=495, Evalue=1e-140,
Organism=Drosophila melanogaster, GI19921738, Length=386, Percent_Identity=51.5544041450777, Blast_Score=415, Evalue=1e-116,
Organism=Drosophila melanogaster, GI45553807, Length=448, Percent_Identity=28.125, Blast_Score=155, Evalue=4e-38,
Organism=Drosophila melanogaster, GI45553816, Length=448, Percent_Identity=28.125, Blast_Score=155, Evalue=4e-38,
Organism=Drosophila melanogaster, GI24651721, Length=448, Percent_Identity=28.125, Blast_Score=155, Evalue=4e-38,
Organism=Drosophila melanogaster, GI17864154, Length=448, Percent_Identity=28.125, Blast_Score=155, Evalue=4e-38,
Organism=Drosophila melanogaster, GI24652838, Length=455, Percent_Identity=28.5714285714286, Blast_Score=148, Evalue=5e-36,
Organism=Drosophila melanogaster, GI17137572, Length=455, Percent_Identity=28.5714285714286, Blast_Score=148, Evalue=5e-36,
Organism=Drosophila melanogaster, GI19922690, Length=305, Percent_Identity=31.8032786885246, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI17137380, Length=340, Percent_Identity=31.1764705882353, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI45550900, Length=441, Percent_Identity=27.6643990929705, Blast_Score=122, Evalue=6e-28,
Organism=Drosophila melanogaster, GI24647048, Length=433, Percent_Identity=27.0207852193995, Blast_Score=102, Evalue=6e-22,
Organism=Drosophila melanogaster, GI24647046, Length=435, Percent_Identity=26.8965517241379, Blast_Score=102, Evalue=6e-22,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 43144; Mature: 43013

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKAKFERTKPHVNVGTIGHVDHGKTTLTAALTKIGAERFGGEFKAYDAIDAAPEEKARG
CCCCCCCCCCCCEEECEEECCCCCCHHHHHHHHHHHHHHHCCCEEECCCCCCCCCHHHCC
ITISTAHVEYESPTRHYAHVDCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHI
EEEEEEEEEECCCCCEEEEEECCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHE
LLSRQVGVPHIVVFLNKADMVDDAELLELVEMEVRELLSKYDFPGDDTPIIHGSARLALD
EEECCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCEEEEEC
GDQSDIGVPAILKLVEALDSFIPEPTRDVDRPFLMPVEDVFSISGRGTVVTGRIERGIIK
CCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCCCCEEEEEEECCCEEE
VGDEIEIVGIRDTQKTTVTGVEMFRKLLDQGQAGDNAGLLLRGTKRDDVERGQVLCKPGS
ECCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCEEEECCCC
IKPHTEFEAEVYVLSKDEGGRHTPFFKGYRPQFYFRTTDITGACQLPEGVEMVMPGDNVK
CCCCCCCCEEEEEEECCCCCCCCCCCCCCCCEEEEEECCCCCEECCCCCCEEEECCCCCE
MVVTLINPVAMDEGLRFAIREGGRTVGAGVVAKIIK
EEEEEECCHHHCCCCEEEECCCCCEEHHHHHHHHCC
>Mature Secondary Structure 
AKAKFERTKPHVNVGTIGHVDHGKTTLTAALTKIGAERFGGEFKAYDAIDAAPEEKARG
CCCCCCCCCCCEEECEEECCCCCCHHHHHHHHHHHHHHHCCCEEECCCCCCCCCHHHCC
ITISTAHVEYESPTRHYAHVDCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHI
EEEEEEEEEECCCCCEEEEEECCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHE
LLSRQVGVPHIVVFLNKADMVDDAELLELVEMEVRELLSKYDFPGDDTPIIHGSARLALD
EEECCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCEEEEEC
GDQSDIGVPAILKLVEALDSFIPEPTRDVDRPFLMPVEDVFSISGRGTVVTGRIERGIIK
CCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCCCCEEEEEEECCCEEE
VGDEIEIVGIRDTQKTTVTGVEMFRKLLDQGQAGDNAGLLLRGTKRDDVERGQVLCKPGS
ECCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCEEEECCCC
IKPHTEFEAEVYVLSKDEGGRHTPFFKGYRPQFYFRTTDITGACQLPEGVEMVMPGDNVK
CCCCCCCCEEEEEEECCCCCCCCCCCCCCCCEEEEEECCCCCEECCCCCCEEEECCCCCE
MVVTLINPVAMDEGLRFAIREGGRTVGAGVVAKIIK
EEEEEECCHHHCCCCEEEECCCCCEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA