Click here to switch to the map view.

The map label for this gene is prsA [H]

Identifier: 21241719

GI number: 21241719

Start: 1112083

End: 1113018

Strand: Direct

Name: prsA [H]

Synonym: XAC0950

Alternate gene names: 21241719

Gene position: 1112083-1113018 (Clockwise)

Preceding gene: 21241718

Following gene: 21241720

Centisome position: 21.49

GC content: 65.06

Gene sequence:

>936_bases
GTGTTCTCCGGCAATGCCAACAAGCCGCTTGCCCAGAGCATCTGCAAGGAACTGGGCGTTCGCATGGGCAAGGCGTTGGT
CACGCGCTTTTCCGACGGCGAAGTGCAGGTGGAGATCGAAGAGAGCGTGCGTCGGCAGGAAGTGTTCGTCATCCAGCCTA
CCTGCGCGCCCAGCGCCGAAAACCTGATGGAACTGCTGGTGCTGATCGACGCGCTCAAGCGCGCCAGCGCCCAGTCCGTT
ACCGCGGTGATCCCGTATTTCGGTTATTCGCGCCAGGACCGTCGCATGCGCTCCTCGCGCGTGCCGATCACTGCCAAGGT
GGCGGCCAAGATGATCTGCGCCATGGAGGCGGACCGCGTGCTGACGGTCGACCTGCACGCCGATCAGATCCAGGGTTTCT
TCGATGTGCCGGTCGACAACGTCTATGCCTCGCCGCTGCTGCTGGCCGACATCTGGCGCGCCTACGGCACCGACAACCTG
ATCGTGGTGTCGCCCGACGTGGGTGGCGTGGTGCGCGCACGCGCCGTCGCCAAGCGCCTGGATGACGCCGATCTGGCCAT
CATCGACAAGCGCCGTCCGCGCGCCAATGTGGCCACCGTGATGAACATCATCGGCGACGTGCAGGGCAAGACCTGCGTGC
TGGTCGACGACCTGGTCGACACCGCCGGTACCTTGTGCGCCGCTGCCGCAGCGCTGAAGCAGCGCGGCGCGCTCAAGGTC
GTGGCCTACATCACCCACCCGGTACTCTCGGGCCCGGCGGTGGACAACATCAACAATTCGCAGCTCGACGAGCTGGTGGT
CACCGACACGATTCCGCTGAGCGAAGCGGCGCGCACCTGCGCCAAGATTCGTCAGCTGAGCGTGGCCGAACTGCTGGCCG
AGACCATCCGCCGTATCGCCTTCGGCGAATCGGTCAGCTCGCTTTACGTCGACTGA

Upstream 100 bases:

>100_bases
AAAGCCCGGCATTGGTTCGGCTTTGTCCTGCTCCACCATCGATCCGCACGCTCTTCGCCGCCGCCCGAGAGATCTCATGC
AAGACCAACGTAATCTGCTG

Downstream 100 bases:

>100_bases
TGCCCCATTCGGTGCCCGGATTCCCGGGTGCCGCCACACGTCTCACCTGGTCGCGGGTGAGACGCTTCATCGCCGCGAGG
CGGTGTTCCAACCAGGTAGT

Product: ribose-phosphate pyrophosphokinase

Products: NA

Alternate protein names: RPPK; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase [H]

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MFSGNANKPLAQSICKELGVRMGKALVTRFSDGEVQVEIEESVRRQEVFVIQPTCAPSAENLMELLVLIDALKRASAQSV
TAVIPYFGYSRQDRRMRSSRVPITAKVAAKMICAMEADRVLTVDLHADQIQGFFDVPVDNVYASPLLLADIWRAYGTDNL
IVVSPDVGGVVRARAVAKRLDDADLAIIDKRRPRANVATVMNIIGDVQGKTCVLVDDLVDTAGTLCAAAAALKQRGALKV
VAYITHPVLSGPAVDNINNSQLDELVVTDTIPLSEAARTCAKIRQLSVAELLAETIRRIAFGESVSSLYVD

Sequences:

>Translated_311_residues
MFSGNANKPLAQSICKELGVRMGKALVTRFSDGEVQVEIEESVRRQEVFVIQPTCAPSAENLMELLVLIDALKRASAQSV
TAVIPYFGYSRQDRRMRSSRVPITAKVAAKMICAMEADRVLTVDLHADQIQGFFDVPVDNVYASPLLLADIWRAYGTDNL
IVVSPDVGGVVRARAVAKRLDDADLAIIDKRRPRANVATVMNIIGDVQGKTCVLVDDLVDTAGTLCAAAAALKQRGALKV
VAYITHPVLSGPAVDNINNSQLDELVVTDTIPLSEAARTCAKIRQLSVAELLAETIRRIAFGESVSSLYVD
>Mature_311_residues
MFSGNANKPLAQSICKELGVRMGKALVTRFSDGEVQVEIEESVRRQEVFVIQPTCAPSAENLMELLVLIDALKRASAQSV
TAVIPYFGYSRQDRRMRSSRVPITAKVAAKMICAMEADRVLTVDLHADQIQGFFDVPVDNVYASPLLLADIWRAYGTDNL
IVVSPDVGGVVRARAVAKRLDDADLAIIDKRRPRANVATVMNIIGDVQGKTCVLVDDLVDTAGTLCAAAAALKQRGALKV
VAYITHPVLSGPAVDNINNSQLDELVVTDTIPLSEAARTCAKIRQLSVAELLAETIRRIAFGESVSSLYVD

Specific function: Utilized by both the de novo and the salvage pathways by which endogenously formed or exogenously added pyrimidine, purine, or pyridine bases are converted to the corresponding ribonucleoside monophosphates. [C]

COG id: COG0462

COG function: function code FE; Phosphoribosylpyrophosphate synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribose-phosphate pyrophosphokinase family [H]

Homologues:

Organism=Homo sapiens, GI4506129, Length=315, Percent_Identity=46.3492063492063, Blast_Score=288, Evalue=3e-78,
Organism=Homo sapiens, GI84875539, Length=317, Percent_Identity=46.0567823343849, Blast_Score=288, Evalue=4e-78,
Organism=Homo sapiens, GI4506127, Length=315, Percent_Identity=46.6666666666667, Blast_Score=288, Evalue=5e-78,
Organism=Homo sapiens, GI28557709, Length=315, Percent_Identity=45.7142857142857, Blast_Score=280, Evalue=1e-75,
Organism=Homo sapiens, GI4506133, Length=343, Percent_Identity=36.734693877551, Blast_Score=192, Evalue=2e-49,
Organism=Homo sapiens, GI194018537, Length=345, Percent_Identity=37.3913043478261, Blast_Score=182, Evalue=3e-46,
Organism=Homo sapiens, GI310128524, Length=145, Percent_Identity=28.9655172413793, Blast_Score=73, Evalue=4e-13,
Organism=Homo sapiens, GI310115209, Length=145, Percent_Identity=28.9655172413793, Blast_Score=73, Evalue=4e-13,
Organism=Homo sapiens, GI310118259, Length=145, Percent_Identity=28.9655172413793, Blast_Score=73, Evalue=4e-13,
Organism=Homo sapiens, GI310119946, Length=145, Percent_Identity=28.9655172413793, Blast_Score=73, Evalue=4e-13,
Organism=Escherichia coli, GI1787458, Length=309, Percent_Identity=62.1359223300971, Blast_Score=399, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI17554704, Length=310, Percent_Identity=45.8064516129032, Blast_Score=276, Evalue=7e-75,
Organism=Caenorhabditis elegans, GI25149168, Length=310, Percent_Identity=45.8064516129032, Blast_Score=276, Evalue=7e-75,
Organism=Caenorhabditis elegans, GI17554702, Length=310, Percent_Identity=45.8064516129032, Blast_Score=276, Evalue=8e-75,
Organism=Caenorhabditis elegans, GI71989924, Length=310, Percent_Identity=45.8064516129032, Blast_Score=275, Evalue=2e-74,
Organism=Caenorhabditis elegans, GI17570245, Length=338, Percent_Identity=34.6153846153846, Blast_Score=194, Evalue=4e-50,
Organism=Saccharomyces cerevisiae, GI6321776, Length=310, Percent_Identity=45.1612903225806, Blast_Score=266, Evalue=4e-72,
Organism=Saccharomyces cerevisiae, GI6319403, Length=312, Percent_Identity=44.2307692307692, Blast_Score=251, Evalue=1e-67,
Organism=Saccharomyces cerevisiae, GI6320946, Length=311, Percent_Identity=43.0868167202572, Blast_Score=246, Evalue=5e-66,
Organism=Saccharomyces cerevisiae, GI6322667, Length=199, Percent_Identity=38.1909547738693, Blast_Score=134, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6324511, Length=115, Percent_Identity=43.4782608695652, Blast_Score=92, Evalue=9e-20,
Organism=Drosophila melanogaster, GI21355239, Length=315, Percent_Identity=46.6666666666667, Blast_Score=276, Evalue=1e-74,
Organism=Drosophila melanogaster, GI45551540, Length=337, Percent_Identity=43.620178041543, Blast_Score=267, Evalue=5e-72,
Organism=Drosophila melanogaster, GI24651458, Length=352, Percent_Identity=34.6590909090909, Blast_Score=192, Evalue=2e-49,
Organism=Drosophila melanogaster, GI24651456, Length=352, Percent_Identity=34.6590909090909, Blast_Score=192, Evalue=2e-49,
Organism=Drosophila melanogaster, GI281362873, Length=352, Percent_Identity=34.6590909090909, Blast_Score=192, Evalue=2e-49,
Organism=Drosophila melanogaster, GI24651454, Length=352, Percent_Identity=34.6590909090909, Blast_Score=192, Evalue=2e-49,
Organism=Drosophila melanogaster, GI24651462, Length=371, Percent_Identity=31.8059299191375, Blast_Score=180, Evalue=1e-45,
Organism=Drosophila melanogaster, GI24651464, Length=371, Percent_Identity=31.8059299191375, Blast_Score=180, Evalue=1e-45,
Organism=Drosophila melanogaster, GI45552010, Length=371, Percent_Identity=31.8059299191375, Blast_Score=179, Evalue=2e-45,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000842
- InterPro:   IPR005946
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00156 Pribosyltran [H]

EC number: =2.7.6.1 [H]

Molecular weight: Translated: 33636; Mature: 33636

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00114 PRPP_SYNTHETASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFSGNANKPLAQSICKELGVRMGKALVTRFSDGEVQVEIEESVRRQEVFVIQPTCAPSAE
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHCEEEEECCCCCCCHH
NLMELLVLIDALKRASAQSVTAVIPYFGYSRQDRRMRSSRVPITAKVAAKMICAMEADRV
HHHHHHHHHHHHHHHCHHHHEEEECCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHCCCCE
LTVDLHADQIQGFFDVPVDNVYASPLLLADIWRAYGTDNLIVVSPDVGGVVRARAVAKRL
EEEEECHHHHCCEECCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHC
DDADLAIIDKRRPRANVATVMNIIGDVQGKTCVLVDDLVDTAGTLCAAAAALKQRGALKV
CCCCEEEEECCCCCHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCEEE
VAYITHPVLSGPAVDNINNSQLDELVVTDTIPLSEAARTCAKIRQLSVAELLAETIRRIA
EEEHHHHHCCCCCCCCCCCHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FGESVSSLYVD
HCCCHHHHCCC
>Mature Secondary Structure
MFSGNANKPLAQSICKELGVRMGKALVTRFSDGEVQVEIEESVRRQEVFVIQPTCAPSAE
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHCEEEEECCCCCCCHH
NLMELLVLIDALKRASAQSVTAVIPYFGYSRQDRRMRSSRVPITAKVAAKMICAMEADRV
HHHHHHHHHHHHHHHCHHHHEEEECCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHCCCCE
LTVDLHADQIQGFFDVPVDNVYASPLLLADIWRAYGTDNLIVVSPDVGGVVRARAVAKRL
EEEEECHHHHCCEECCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHC
DDADLAIIDKRRPRANVATVMNIIGDVQGKTCVLVDDLVDTAGTLCAAAAALKQRGALKV
CCCCEEEEECCCCCHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCEEE
VAYITHPVLSGPAVDNINNSQLDELVVTDTIPLSEAARTCAKIRQLSVAELLAETIRRIA
EEEHHHHHCCCCCCCCCCCHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FGESVSSLYVD
HCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]