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The map label for this gene is prfA

Identifier: 21241714

GI number: 21241714

Start: 1106178

End: 1107263

Strand: Reverse

Name: prfA

Synonym: XAC0944

Alternate gene names: 21241714

Gene position: 1107263-1106178 (Counterclockwise)

Preceding gene: 21241715

Following gene: 21241713

Centisome position: 21.39

GC content: 65.38

Gene sequence:

>1086_bases
ATGACGCCGACCCTGCGCCGTAAGCTCCAAGCGCTGGCCGAGCGCCGCGAAGAACTGCAGCACCTGCTCTCCGACCCCGA
TGTGGTGGGCAACAACGACACGTTCCGTACGCTCTCGCGCGAACTGTCGCAACTGGAGCCGGTGGCGGTGGCGCTGGAAG
AGGAGGCGCGCGCCAAGGCCGACCTGGCCACGGCCGAAGCCCTGCGCAACGACCCGGAGATGCGCGAGCTGGCCGAAGAG
GAAATTGCCGCTGCACAGGCGCGCCTGGAACAACTCGATGCACAGCTGGCATCGCTGCTGGTACCGCGCGACCCGCGCGA
CGACGGCAACCTGTTTCTGGAAGTGCGCGCGGGCACTGGCGGTGACGAAGCGGCGATCTTTGCCGGCGACCTGTTCCGCA
TGTACGCACGCTACGCCGAGCGCCAGGGCTGGAAGGTGGAAATCGAATCCGACAGTCCCGGCGAGCACGGCGGCTACAAG
GAAGTGGTGGCGCGGGTGGTCGGGCGCGGTGCGTATTCGCGGCTGAAATTCGAATCCGGCACCCATCGCGTGCAACGCGT
GCCGGCAACCGAATCGCAAGGCCGTATCCACACCTCGGCCGCAACGGTGGCGATCATTCCCGAAGCCGACGACGTGGAAG
AGATCACGATCAATCCAGCCGATCTGAAGGTGGACACGTTCCGCTCGTCCGGTGCGGGCGGCCAGCACGTCAACAAGACC
GAATCGGCGATCCGCATCACCCACGTGCCCAGCGGGGTGGTGGTGGAATGCCAGACCGAGCGCAGCCAGCATGCCAATCG
CGACAAGGCGATGAAGCGGTTGAAGGCGCAGTTGCTGGACACCGAACGCAGCAAGGCGGCAGCGGCCGAAGCGCAGACGC
GCAAGTTGCAGGTGGGCAGCGGCGACCGCAGCCAGCGCATTCGCACCTATAGCTTTCCGCAGGGTCGCATCACCGACCAT
CGCGTCGAAGGGCTGACCTTGTACGACCTGCCCAACATCATCGAAGGCGATCTGGATGCATTGATCGGGCGGCTGCTGCA
CGAACATCAAGCCGACGAACTGGCACGGTTGAGCGATAGCCCGTGA

Upstream 100 bases:

>100_bases
TCAACAACGACCTGGAATTGACCGCTGCGGCCGACCGGCTGTTTCCGGAAAAGCCGGGTTACCAACATCCCCCCCATAGC
TACCCCGATCGTGAGGACCG

Downstream 100 bases:

>100_bases
GTGCGCAAGTCCCGCGCGAGCTGCTGCAGCTGCGCGAGCAAGTGCGACGCCAGCCTGGCGATTTCGTCGCCTGGTTGATG
CTGGCCGATGCCGAGCTGGG

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1

Number of amino acids: Translated: 361; Mature: 360

Protein sequence:

>361_residues
MTPTLRRKLQALAERREELQHLLSDPDVVGNNDTFRTLSRELSQLEPVAVALEEEARAKADLATAEALRNDPEMRELAEE
EIAAAQARLEQLDAQLASLLVPRDPRDDGNLFLEVRAGTGGDEAAIFAGDLFRMYARYAERQGWKVEIESDSPGEHGGYK
EVVARVVGRGAYSRLKFESGTHRVQRVPATESQGRIHTSAATVAIIPEADDVEEITINPADLKVDTFRSSGAGGQHVNKT
ESAIRITHVPSGVVVECQTERSQHANRDKAMKRLKAQLLDTERSKAAAAEAQTRKLQVGSGDRSQRIRTYSFPQGRITDH
RVEGLTLYDLPNIIEGDLDALIGRLLHEHQADELARLSDSP

Sequences:

>Translated_361_residues
MTPTLRRKLQALAERREELQHLLSDPDVVGNNDTFRTLSRELSQLEPVAVALEEEARAKADLATAEALRNDPEMRELAEE
EIAAAQARLEQLDAQLASLLVPRDPRDDGNLFLEVRAGTGGDEAAIFAGDLFRMYARYAERQGWKVEIESDSPGEHGGYK
EVVARVVGRGAYSRLKFESGTHRVQRVPATESQGRIHTSAATVAIIPEADDVEEITINPADLKVDTFRSSGAGGQHVNKT
ESAIRITHVPSGVVVECQTERSQHANRDKAMKRLKAQLLDTERSKAAAAEAQTRKLQVGSGDRSQRIRTYSFPQGRITDH
RVEGLTLYDLPNIIEGDLDALIGRLLHEHQADELARLSDSP
>Mature_360_residues
TPTLRRKLQALAERREELQHLLSDPDVVGNNDTFRTLSRELSQLEPVAVALEEEARAKADLATAEALRNDPEMRELAEEE
IAAAQARLEQLDAQLASLLVPRDPRDDGNLFLEVRAGTGGDEAAIFAGDLFRMYARYAERQGWKVEIESDSPGEHGGYKE
VVARVVGRGAYSRLKFESGTHRVQRVPATESQGRIHTSAATVAIIPEADDVEEITINPADLKVDTFRSSGAGGQHVNKTE
SAIRITHVPSGVVVECQTERSQHANRDKAMKRLKAQLLDTERSKAAAAEAQTRKLQVGSGDRSQRIRTYSFPQGRITDHR
VEGLTLYDLPNIIEGDLDALIGRLLHEHQADELARLSDSP

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=277, Percent_Identity=48.3754512635379, Blast_Score=272, Evalue=3e-73,
Organism=Homo sapiens, GI34577120, Length=368, Percent_Identity=36.6847826086957, Blast_Score=243, Evalue=2e-64,
Organism=Homo sapiens, GI166795305, Length=179, Percent_Identity=49.7206703910614, Blast_Score=186, Evalue=3e-47,
Organism=Escherichia coli, GI1787462, Length=359, Percent_Identity=60.1671309192201, Blast_Score=422, Evalue=1e-119,
Organism=Escherichia coli, GI2367172, Length=334, Percent_Identity=39.8203592814371, Blast_Score=226, Evalue=3e-60,
Organism=Caenorhabditis elegans, GI17542784, Length=321, Percent_Identity=34.2679127725857, Blast_Score=186, Evalue=2e-47,
Organism=Saccharomyces cerevisiae, GI6321295, Length=286, Percent_Identity=45.4545454545455, Blast_Score=238, Evalue=1e-63,
Organism=Drosophila melanogaster, GI19921226, Length=338, Percent_Identity=41.4201183431953, Blast_Score=243, Evalue=1e-64,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RF1_XANAC (Q8PNU5)

Other databases:

- EMBL:   AE008923
- RefSeq:   NP_641296.1
- ProteinModelPortal:   Q8PNU5
- SMR:   Q8PNU5
- GeneID:   1155015
- GenomeReviews:   AE008923_GR
- KEGG:   xac:XAC0944
- NMPDR:   fig|190486.1.peg.940
- HOGENOM:   HBG629764
- OMA:   SEQGGYK
- ProtClustDB:   PRK00591
- BioCyc:   XAXO190486:XAC0944-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00093
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373
- SMART:   SM00937
- TIGRFAMs:   TIGR00019

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 39877; Mature: 39746

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPTLRRKLQALAERREELQHLLSDPDVVGNNDTFRTLSRELSQLEPVAVALEEEARAKA
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHH
DLATAEALRNDPEMRELAEEEIAAAQARLEQLDAQLASLLVPRDPRDDGNLFLEVRAGTG
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCC
GDEAAIFAGDLFRMYARYAERQGWKVEIESDSPGEHGGYKEVVARVVGRGAYSRLKFESG
CCCCEEHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHCCCHHHHEEECCC
THRVQRVPATESQGRIHTSAATVAIIPEADDVEEITINPADLKVDTFRSSGAGGQHVNKT
CHHHHCCCCCCCCCEEEECCEEEEEECCCCCCCEEEECCCCEEEECCCCCCCCCCCCCCC
ESAIRITHVPSGVVVECQTERSQHANRDKAMKRLKAQLLDTERSKAAAAEAQTRKLQVGS
CCEEEEEECCCCEEEEEECHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCC
GDRSQRIRTYSFPQGRITDHRVEGLTLYDLPNIIEGDLDALIGRLLHEHQADELARLSDS
CCCCCCEEEECCCCCCCCCCCCCCEEEECCCCHHHCCHHHHHHHHHHHHHHHHHHHCCCC
P
C
>Mature Secondary Structure 
TPTLRRKLQALAERREELQHLLSDPDVVGNNDTFRTLSRELSQLEPVAVALEEEARAKA
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHH
DLATAEALRNDPEMRELAEEEIAAAQARLEQLDAQLASLLVPRDPRDDGNLFLEVRAGTG
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCC
GDEAAIFAGDLFRMYARYAERQGWKVEIESDSPGEHGGYKEVVARVVGRGAYSRLKFESG
CCCCEEHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHCCCHHHHEEECCC
THRVQRVPATESQGRIHTSAATVAIIPEADDVEEITINPADLKVDTFRSSGAGGQHVNKT
CHHHHCCCCCCCCCEEEECCEEEEEECCCCCCCEEEECCCCEEEECCCCCCCCCCCCCCC
ESAIRITHVPSGVVVECQTERSQHANRDKAMKRLKAQLLDTERSKAAAAEAQTRKLQVGS
CCEEEEEECCCCEEEEEECHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCC
GDRSQRIRTYSFPQGRITDHRVEGLTLYDLPNIIEGDLDALIGRLLHEHQADELARLSDS
CCCCCCEEEECCCCCCCCCCCCCCEEEECCCCHHHCCHHHHHHHHHHHHHHHHHHHCCCC
P
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217