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The map label for this gene is ksgA

Identifier: 21241633

GI number: 21241633

Start: 1024298

End: 1025086

Strand: Reverse

Name: ksgA

Synonym: XAC0863

Alternate gene names: 21241633

Gene position: 1025086-1024298 (Counterclockwise)

Preceding gene: 21241634

Following gene: 21241632

Centisome position: 19.81

GC content: 65.27

Gene sequence:

>789_bases
ATGAATTCTTCTTTCAGCGCACCGGCCAAGAAGTCGCTTGGCCAGCACTTTCTTGCCGACCGGTATTACATCGACCGCAT
CGTGCAGGCGGTAGACCCGCGCGCCGGCCAGCATCTGGTCGAGATCGGCCCGGGCCAGGGTGCCATCACCTTTCCGTTGC
TGCGCAAGCATGGCGCGCTGACCGTCATCGAATTCGATCGCGACCTGATCGCGCCGTTGACCGAAGCCGCGGCACCGATC
GGCGCATTGCGCATCATCCACCGCGATGTGCTCAGCGTGGATTTCACTGCACTGGCCGATGGCACGCCGATCCGCCTGGT
CGGCAACCTGCCCTACAACATTTCCTCGCCGATCCTGTTCCATGCGCTGGACCATGCCGCTGCCGTGGCCGACATGCACT
TCATGCTGCAAAAGGAAGTGGTCGACCGCATGGCCGCCGGCCCCGGCAGCAAGGTGTACGGACGGTTGAGCGTGATGCTG
CAGGCGTATTGCGAGGTGACTGCGCTGTTCGTAGTGCCGCCGGGCGCATTCCGGCCGCCGCCGAAGGTGGACTCGGCAGT
GGTGCGGCTGGTGCCGCGCGATCCGGCCACGGTGCTGATCAACGACCGCCGCCGCTTCGCCGATGTGGTGCGCGCCGGGT
TCGGGCAGCGCCGCAAGACCCTGCGCAATGCCTTGTCCGCCATTTGCGAACCGGCCCACTTCGACGCTGCGCAGGTGCGC
CCGGATGCACGCGCCGAACAACTTGAGGTCGCCGATTTCATCCGGCTGGCCAATGTCGAGCTGGCTTGA

Upstream 100 bases:

>100_bases
CGCTGCGGGCATGCCGCATGATGCACGCCGCCTATCCGCCAAGCGCGGTGCGCGCGCAAACGACACCGCGTGCAGGCGCG
GCCTCCGTTAAACTGCGCAG

Downstream 100 bases:

>100_bases
GCGCCGTTCCCACACCGTTTTATTTAGACTTTCGCCATGCAAGATGATCCGCGCTACCGGGTCGAGGTCGAGGTATCGCC
CCGCTTCCTCGCCCATCAAT

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase

Number of amino acids: Translated: 262; Mature: 262

Protein sequence:

>262_residues
MNSSFSAPAKKSLGQHFLADRYYIDRIVQAVDPRAGQHLVEIGPGQGAITFPLLRKHGALTVIEFDRDLIAPLTEAAAPI
GALRIIHRDVLSVDFTALADGTPIRLVGNLPYNISSPILFHALDHAAAVADMHFMLQKEVVDRMAAGPGSKVYGRLSVML
QAYCEVTALFVVPPGAFRPPPKVDSAVVRLVPRDPATVLINDRRRFADVVRAGFGQRRKTLRNALSAICEPAHFDAAQVR
PDARAEQLEVADFIRLANVELA

Sequences:

>Translated_262_residues
MNSSFSAPAKKSLGQHFLADRYYIDRIVQAVDPRAGQHLVEIGPGQGAITFPLLRKHGALTVIEFDRDLIAPLTEAAAPI
GALRIIHRDVLSVDFTALADGTPIRLVGNLPYNISSPILFHALDHAAAVADMHFMLQKEVVDRMAAGPGSKVYGRLSVML
QAYCEVTALFVVPPGAFRPPPKVDSAVVRLVPRDPATVLINDRRRFADVVRAGFGQRRKTLRNALSAICEPAHFDAAQVR
PDARAEQLEVADFIRLANVELA
>Mature_262_residues
MNSSFSAPAKKSLGQHFLADRYYIDRIVQAVDPRAGQHLVEIGPGQGAITFPLLRKHGALTVIEFDRDLIAPLTEAAAPI
GALRIIHRDVLSVDFTALADGTPIRLVGNLPYNISSPILFHALDHAAAVADMHFMLQKEVVDRMAAGPGSKVYGRLSVML
QAYCEVTALFVVPPGAFRPPPKVDSAVVRLVPRDPATVLINDRRRFADVVRAGFGQRRKTLRNALSAICEPAHFDAAQVR
PDARAEQLEVADFIRLANVELA

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily

Homologues:

Organism=Homo sapiens, GI156415992, Length=285, Percent_Identity=32.6315789473684, Blast_Score=116, Evalue=2e-26,
Organism=Homo sapiens, GI7657198, Length=275, Percent_Identity=32, Blast_Score=116, Evalue=2e-26,
Organism=Escherichia coli, GI1786236, Length=252, Percent_Identity=48.8095238095238, Blast_Score=241, Evalue=4e-65,
Organism=Caenorhabditis elegans, GI25146882, Length=219, Percent_Identity=33.7899543378995, Blast_Score=108, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI25141369, Length=246, Percent_Identity=28.4552845528455, Blast_Score=84, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6324989, Length=212, Percent_Identity=29.7169811320755, Blast_Score=94, Evalue=2e-20,
Organism=Drosophila melanogaster, GI21357273, Length=285, Percent_Identity=33.6842105263158, Blast_Score=114, Evalue=5e-26,
Organism=Drosophila melanogaster, GI21358017, Length=216, Percent_Identity=33.7962962962963, Blast_Score=109, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMA_XANAC (Q8PP25)

Other databases:

- EMBL:   AE008923
- RefSeq:   NP_641215.1
- ProteinModelPortal:   Q8PP25
- SMR:   Q8PP25
- GeneID:   1154934
- GenomeReviews:   AE008923_GR
- KEGG:   xac:XAC0863
- NMPDR:   fig|190486.1.peg.859
- HOGENOM:   HBG319664
- OMA:   GVLCGWR
- ProtClustDB:   PRK00274
- BioCyc:   XAXO190486:XAC0863-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00607
- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530
- Gene3D:   G3DSA:1.10.8.100
- PANTHER:   PTHR11727
- SMART:   SM00650
- TIGRFAMs:   TIGR00755

Pfam domain/function: PF00398 RrnaAD

EC number: =2.1.1.182

Molecular weight: Translated: 28554; Mature: 28554

Theoretical pI: Translated: 9.13; Mature: 9.13

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: BINDING 16-16 BINDING 18-18 BINDING 43-43 BINDING 64-64 BINDING 89-89 BINDING 109-109

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSSFSAPAKKSLGQHFLADRYYIDRIVQAVDPRAGQHLVEIGPGQGAITFPLLRKHGAL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCEEHHHHHCCCCE
TVIEFDRDLIAPLTEAAAPIGALRIIHRDVLSVDFTALADGTPIRLVGNLPYNISSPILF
EEEECCCHHHHHHHHHHCHHHHHHHHHHHHHHCCHHHCCCCCCEEEEECCCCCCCCCHHH
HALDHAAAVADMHFMLQKEVVDRMAAGPGSKVYGRLSVMLQAYCEVTALFVVPPGAFRPP
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHEEEEEEECCCCCCCC
PKVDSAVVRLVPRDPATVLINDRRRFADVVRAGFGQRRKTLRNALSAICEPAHFDAAQVR
CCCCCHHEEEECCCCCEEEECCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCHHHCC
PDARAEQLEVADFIRLANVELA
CCCHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNSSFSAPAKKSLGQHFLADRYYIDRIVQAVDPRAGQHLVEIGPGQGAITFPLLRKHGAL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCEEHHHHHCCCCE
TVIEFDRDLIAPLTEAAAPIGALRIIHRDVLSVDFTALADGTPIRLVGNLPYNISSPILF
EEEECCCHHHHHHHHHHCHHHHHHHHHHHHHHCCHHHCCCCCCEEEEECCCCCCCCCHHH
HALDHAAAVADMHFMLQKEVVDRMAAGPGSKVYGRLSVMLQAYCEVTALFVVPPGAFRPP
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHEEEEEEECCCCCCCC
PKVDSAVVRLVPRDPATVLINDRRRFADVVRAGFGQRRKTLRNALSAICEPAHFDAAQVR
CCCCCHHEEEECCCCCEEEECCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCHHHCC
PDARAEQLEVADFIRLANVELA
CCCHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217