Click here to switch to the map view.

The map label for this gene is ribH

Identifier: 21241521

GI number: 21241521

Start: 890520

End: 890984

Strand: Direct

Name: ribH

Synonym: XAC0750

Alternate gene names: 21241521

Gene position: 890520-890984 (Clockwise)

Preceding gene: 21241520

Following gene: 21241522

Centisome position: 17.21

GC content: 66.24

Gene sequence:

>465_bases
ATGACCCACTACGAAGGCGATCTTCGCCCCACCACCGCGCGCTTTGCGATCATCGCCAGCCGCTGGAATGCCCGTATCAC
CGACGTGCTGGTTGCCGGCGCGCGCCAGAGCCTGGCCGGCAACGGCATCGGCGAAGACGCCATCGACGTGATCCGCGTGC
CGGGCGCCTGGGAGATCCCGATCGCCGCCAATCGCGTGGCGCAGTCCGGTCAGCACGGCGCGATCATCGCGCTGGGCTGC
GTGATTCGTGGCGATACCCGCCATTACGAGCACGTGGCCGACCTGTGTGCCGAAGGCCTGATGAGCGTGCAGTTGCAGAC
CGGCGTGCCGGTGCTCAATGGCGTGCTGGCGGTCGAACGCGTGGAAGATGCCGAAGCCCGTGCTGGCGGCAGCCACGGCA
ATAAGGGCGAGGAATGCGCGCTTGCAGCGCTGGAACTGGTGAATTTGATGGAGTTGCTGCCATGA

Upstream 100 bases:

>100_bases
CGCATGGCCGGGTCTGCTTTGGCCGACCCGGCCCACACGCGTCGCCAGGCCTGGGCCCGGCAGCTGTTAAACTTCCCGCC
CCCTCGAGTTGCCGACCCGC

Downstream 100 bases:

>100_bases
GCAAGCCCGGTGGGCATTCGCGCCACGGCCGTCGCGACGGCATCGACCCGGTGCTGCGGTCGCGCGCACGTCGGCGCGCC
TTGCAGGCGGTCTATGCCTG

Product: 6,7-dimethyl-8-ribityllumazine synthase

Products: NA

Alternate protein names: DMRL synthase; Lumazine synthase; Riboflavin synthase beta chain

Number of amino acids: Translated: 154; Mature: 153

Protein sequence:

>154_residues
MTHYEGDLRPTTARFAIIASRWNARITDVLVAGARQSLAGNGIGEDAIDVIRVPGAWEIPIAANRVAQSGQHGAIIALGC
VIRGDTRHYEHVADLCAEGLMSVQLQTGVPVLNGVLAVERVEDAEARAGGSHGNKGEECALAALELVNLMELLP

Sequences:

>Translated_154_residues
MTHYEGDLRPTTARFAIIASRWNARITDVLVAGARQSLAGNGIGEDAIDVIRVPGAWEIPIAANRVAQSGQHGAIIALGC
VIRGDTRHYEHVADLCAEGLMSVQLQTGVPVLNGVLAVERVEDAEARAGGSHGNKGEECALAALELVNLMELLP
>Mature_153_residues
THYEGDLRPTTARFAIIASRWNARITDVLVAGARQSLAGNGIGEDAIDVIRVPGAWEIPIAANRVAQSGQHGAIIALGCV
IRGDTRHYEHVADLCAEGLMSVQLQTGVPVLNGVLAVERVEDAEARAGGSHGNKGEECALAALELVNLMELLP

Specific function: Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)- ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The beta subunit catalyzes

COG id: COG0054

COG function: function code H; Riboflavin synthase beta-chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DMRL synthase family

Homologues:

Organism=Escherichia coli, GI1786617, Length=152, Percent_Identity=43.421052631579, Blast_Score=118, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6324429, Length=152, Percent_Identity=35.5263157894737, Blast_Score=85, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RISB_XANAC (Q8PPD6)

Other databases:

- EMBL:   AE008923
- RefSeq:   NP_641103.1
- ProteinModelPortal:   Q8PPD6
- SMR:   Q8PPD6
- GeneID:   1154821
- GenomeReviews:   AE008923_GR
- KEGG:   xac:XAC0750
- HOGENOM:   HBG311126
- OMA:   KAGNKGW
- ProtClustDB:   PRK00061
- BioCyc:   XAXO190486:XAC0750-MONOMER
- BRENDA:   2.5.1.9
- HAMAP:   MF_00178
- InterPro:   IPR002180
- Gene3D:   G3DSA:3.40.50.960
- PANTHER:   PTHR21058
- TIGRFAMs:   TIGR00114

Pfam domain/function: PF00885 DMRL_synthase; SSF52121 DMRL_synthase

EC number: =2.5.1.9

Molecular weight: Translated: 16242; Mature: 16111

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHYEGDLRPTTARFAIIASRWNARITDVLVAGARQSLAGNGIGEDAIDVIRVPGAWEIP
CCCCCCCCCCCHHHHEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCHHEEECCCCEECC
IAANRVAQSGQHGAIIALGCVIRGDTRHYEHVADLCAEGLMSVQLQTGVPVLNGVLAVER
HHHHHHHCCCCCCEEEEEEEEEECCCHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHH
VEDAEARAGGSHGNKGEECALAALELVNLMELLP
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
THYEGDLRPTTARFAIIASRWNARITDVLVAGARQSLAGNGIGEDAIDVIRVPGAWEIP
CCCCCCCCCCHHHHEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCHHEEECCCCEECC
IAANRVAQSGQHGAIIALGCVIRGDTRHYEHVADLCAEGLMSVQLQTGVPVLNGVLAVER
HHHHHHHCCCCCCEEEEEEEEEECCCHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHH
VEDAEARAGGSHGNKGEECALAALELVNLMELLP
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217