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The map label for this gene is purR [H]

Identifier: 21241484

GI number: 21241484

Start: 847117

End: 848184

Strand: Direct

Name: purR [H]

Synonym: XAC0713

Alternate gene names: 21241484

Gene position: 847117-848184 (Clockwise)

Preceding gene: 21241483

Following gene: 77748548

Centisome position: 16.37

GC content: 65.82

Gene sequence:

>1068_bases
ATGCGCAGGCCCACCATCAAAGATGTCGCCGAGCGCGCCAAGGTCTCGTTGAAGACCGTGTCGCGGGTGATCAACAACGA
GCCATCGGTGATGCAGGCCACGCGTGCACGCGTGCTGCGCGCCATCGCCGATCTCGACTACGAACCCGATCCGTCCGCGC
GCAATCTGCGCAGCGGCACGCCGTTCGTGATCGGCCTGGTATACGACAACCCCAACCCGTACCACATCATCGGCATTCAG
AATGGCGTGCTGGCCGCGTGCCGCGAAACCGGCTTCGGCTTGCAGATCCATCCCTGCGATTCCACCTCGCCGTTGCTGGC
CGAAGAACTGGCCGAATGGGTGCAGCGCTCGCGACTGGCCGGCGTGGTGTTGACCGCGCCGATGTCCGAGCGCCCGGAAT
TGCTGGCCGGTCTGGCCGCGCGCGGCATCAAGAGCGTGCGCATCATCGCCTCCACCGACGACCCCGGCGATGGCCCGTGC
GTGTATATCGACGACCGCGATGCCGCGTATGAAATCACCGAGCACCTGATCCAGCTCGGCCATCAACGCATCGGCTTCCT
GTGGGGCGGCCCGCAGCATCGCTCCAGCGGCGAACGTTACGCCGGCTATGAAGCGGCGTTGAAGGATTACGGCATCAGCC
TGGACAAGCACCTGGTGATTCCCGGCGACTACACGTTCGACGATGGCTTCCGTGGCGCACGCCGGCTGTTGTCGTTGCGC
GAGCCGCCCACCGCCATCTTCGGCAGCAACGACGAGATCGCTGCAGGCGTGCTGGCCGCGGCCAAGTCCACCGGCATGAA
CGTGCCGTATCAATTGTCGATCGCCGGGTTCGAAGACAGCCCGTTCTCGCGCCAGTCGTGGCCGGCACTGACCACTGCCA
AGCAGGCCACCGACGACATCGCGCGGCATGCCGCACGCTTGTTGATCAGCCAGCTGCGCAGCGATGCCTACGACGACCAA
CCGGCGCAATTGCAGAGCCGCGGCTTCGTGCCGCAGTTGGTGGTGCGCGGCTCCACCGCGCCGGCGCCTGCGTCCACCGG
CAAACCCCTTCCTCCTGAATCTGCCTGA

Upstream 100 bases:

>100_bases
TACATCCTGTTCTATTCGCTGCGCGGTCATCGCGTGGGCCTGCCGGCCCAGGCCAAGTGATCGCGGCGGCATGATGCGTT
CCGACGGACAAGGTACATCC

Downstream 100 bases:

>100_bases
GCCACGATGAGCCTTCCCTCCGAAACCGAGACCTTGATGTTCCGCGAAGCGGCGCAGACCGCCGATGTGGTCGCTGCACA
GTTCGCACGTAATGCCGACA

Product: LacI family transcription regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 355; Mature: 355

Protein sequence:

>355_residues
MRRPTIKDVAERAKVSLKTVSRVINNEPSVMQATRARVLRAIADLDYEPDPSARNLRSGTPFVIGLVYDNPNPYHIIGIQ
NGVLAACRETGFGLQIHPCDSTSPLLAEELAEWVQRSRLAGVVLTAPMSERPELLAGLAARGIKSVRIIASTDDPGDGPC
VYIDDRDAAYEITEHLIQLGHQRIGFLWGGPQHRSSGERYAGYEAALKDYGISLDKHLVIPGDYTFDDGFRGARRLLSLR
EPPTAIFGSNDEIAAGVLAAAKSTGMNVPYQLSIAGFEDSPFSRQSWPALTTAKQATDDIARHAARLLISQLRSDAYDDQ
PAQLQSRGFVPQLVVRGSTAPAPASTGKPLPPESA

Sequences:

>Translated_355_residues
MRRPTIKDVAERAKVSLKTVSRVINNEPSVMQATRARVLRAIADLDYEPDPSARNLRSGTPFVIGLVYDNPNPYHIIGIQ
NGVLAACRETGFGLQIHPCDSTSPLLAEELAEWVQRSRLAGVVLTAPMSERPELLAGLAARGIKSVRIIASTDDPGDGPC
VYIDDRDAAYEITEHLIQLGHQRIGFLWGGPQHRSSGERYAGYEAALKDYGISLDKHLVIPGDYTFDDGFRGARRLLSLR
EPPTAIFGSNDEIAAGVLAAAKSTGMNVPYQLSIAGFEDSPFSRQSWPALTTAKQATDDIARHAARLLISQLRSDAYDDQ
PAQLQSRGFVPQLVVRGSTAPAPASTGKPLPPESA
>Mature_355_residues
MRRPTIKDVAERAKVSLKTVSRVINNEPSVMQATRARVLRAIADLDYEPDPSARNLRSGTPFVIGLVYDNPNPYHIIGIQ
NGVLAACRETGFGLQIHPCDSTSPLLAEELAEWVQRSRLAGVVLTAPMSERPELLAGLAARGIKSVRIIASTDDPGDGPC
VYIDDRDAAYEITEHLIQLGHQRIGFLWGGPQHRSSGERYAGYEAALKDYGISLDKHLVIPGDYTFDDGFRGARRLLSLR
EPPTAIFGSNDEIAAGVLAAAKSTGMNVPYQLSIAGFEDSPFSRQSWPALTTAKQATDDIARHAARLLISQLRSDAYDDQ
PAQLQSRGFVPQLVVRGSTAPAPASTGKPLPPESA

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790369, Length=341, Percent_Identity=32.258064516129, Blast_Score=151, Evalue=6e-38,
Organism=Escherichia coli, GI1787948, Length=342, Percent_Identity=30.7017543859649, Blast_Score=134, Evalue=7e-33,
Organism=Escherichia coli, GI1790194, Length=344, Percent_Identity=31.1046511627907, Blast_Score=131, Evalue=6e-32,
Organism=Escherichia coli, GI1789202, Length=290, Percent_Identity=32.4137931034483, Blast_Score=127, Evalue=8e-31,
Organism=Escherichia coli, GI1789068, Length=292, Percent_Identity=30.4794520547945, Blast_Score=118, Evalue=5e-28,
Organism=Escherichia coli, GI1788474, Length=343, Percent_Identity=30.6122448979592, Blast_Score=117, Evalue=9e-28,
Organism=Escherichia coli, GI1787580, Length=319, Percent_Identity=28.2131661442006, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1786540, Length=295, Percent_Identity=26.4406779661017, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1789456, Length=350, Percent_Identity=28, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI48994940, Length=321, Percent_Identity=24.2990654205607, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1790715, Length=330, Percent_Identity=23.3333333333333, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1787906, Length=288, Percent_Identity=26.7361111111111, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1790689, Length=312, Percent_Identity=26.6025641025641, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 38447; Mature: 38447

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRPTIKDVAERAKVSLKTVSRVINNEPSVMQATRARVLRAIADLDYEPDPSARNLRSGT
CCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
PFVIGLVYDNPNPYHIIGIQNGVLAACRETGFGLQIHPCDSTSPLLAEELAEWVQRSRLA
CEEEEEEECCCCCEEEEEECCCHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHCC
GVVLTAPMSERPELLAGLAARGIKSVRIIASTDDPGDGPCVYIDDRDAAYEITEHLIQLG
CEEEECCCCCCHHHHHHHHHCCCCEEEEEEECCCCCCCCEEEECCCCHHHHHHHHHHHHC
HQRIGFLWGGPQHRSSGERYAGYEAALKDYGISLDKHLVIPGDYTFDDGFRGARRLLSLR
HHHEEEEECCCCCCCCCCCCCCHHHHHHHCCCCCCCEEEECCCCCCCCCHHHHHHHHHCC
EPPTAIFGSNDEIAAGVLAAAKSTGMNVPYQLSIAGFEDSPFSRQSWPALTTAKQATDDI
CCCCEEECCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHH
ARHAARLLISQLRSDAYDDQPAQLQSRGFVPQLVVRGSTAPAPASTGKPLPPESA
HHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRRPTIKDVAERAKVSLKTVSRVINNEPSVMQATRARVLRAIADLDYEPDPSARNLRSGT
CCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
PFVIGLVYDNPNPYHIIGIQNGVLAACRETGFGLQIHPCDSTSPLLAEELAEWVQRSRLA
CEEEEEEECCCCCEEEEEECCCHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHCC
GVVLTAPMSERPELLAGLAARGIKSVRIIASTDDPGDGPCVYIDDRDAAYEITEHLIQLG
CEEEECCCCCCHHHHHHHHHCCCCEEEEEEECCCCCCCCEEEECCCCHHHHHHHHHHHHC
HQRIGFLWGGPQHRSSGERYAGYEAALKDYGISLDKHLVIPGDYTFDDGFRGARRLLSLR
HHHEEEEECCCCCCCCCCCCCCHHHHHHHCCCCCCCEEEECCCCCCCCCHHHHHHHHHCC
EPPTAIFGSNDEIAAGVLAAAKSTGMNVPYQLSIAGFEDSPFSRQSWPALTTAKQATDDI
CCCCEEECCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHH
ARHAARLLISQLRSDAYDDQPAQLQSRGFVPQLVVRGSTAPAPASTGKPLPPESA
HHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA