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The map label for this gene is zraS [H]

Identifier: 21241456

GI number: 21241456

Start: 811027

End: 812529

Strand: Direct

Name: zraS [H]

Synonym: XAC0685

Alternate gene names: 21241456

Gene position: 811027-812529 (Clockwise)

Preceding gene: 21241455

Following gene: 21241457

Centisome position: 15.67

GC content: 65.34

Gene sequence:

>1503_bases
ATGCCGCATGAGGGGGGCAACCTGGAGCAGCTGAAGATCCTTCTGGTGGAAGATTCACCGGAAGACGCCGAGCTGTTGTC
CGATCAGTTGCTCGAGGCCGGGCTGGATGCGGCATTCGAGCGCGTGGACAGCGAGCCGTCGCTGCGCGCCGCGCTGGACG
ACTTCCAGCCGGACATCGTGCTGTCGGACCTGAGCATGCCGGGCTTTTCCGGACACCAGGCGCTGCGCCTGGTGCGCCAG
AACGGTGCCACACCCTTCATCTTCGTCTCCGGCACCATGGGTGAGGAGACCGCGGTCAAGGCGCTGCAGGATGGCGCCAA
CGACTACATCATCAAGCACAACCCGACGCGCTTGCCGAGCGCGGTGATCCGCGCGATCCGCGAGGCGCGTGCGGATCTGG
AACGCCAGCGCGTGGAAAGCGAGCTGATGCGCGCGCAACGTCTGGAAAGCCTGGCGATGCTCGCGGCCGGTTTGAGTCAC
GACCTGCGCAATATCCTGCAACCGTTGTTGATCGTGCCCGACCTGCTGGCCGGGCGCACCGACGACCCGCAGCTGCGTCA
GCTGGCCAACGTGGTGGCCGAATGCGGCCGGCGCGGGCATGAGATGGCCGAGTCGATGCTCTCGTTCGTGCGCGGTTCCA
ACAAGCCGCGCGAGCAGGTCTCCATCGCCAATCTGTTCCAGGCCGTGCAGATGCTGCTCAAGAGCAGCCTGCCCGATGGG
GTACGCCTGCAGATGGACACCATCGCCGCGGATCTGACCATCGAGGCCAACTACACCGAATTGCAGCAATGCCTGCTCAA
CCTTGGCTTGAACGCGATCCAGGCGATGCCCGAGGGCGGCAGACTGATCCTGGCAGCCGATCGCTACGATGCCTCGCGCG
TGCGCATGAGCGTGGCCGACACCGGCGTGGGCATGAGCGATGAAACCCGCGCGCGCCTGTTCAGCCCTTTCTTCACCACC
AAGGCCGATGGCACCGGGTTGGGACTGATTTCGTGCAAGCGCATCATCGAAAGCTATGGCGGCAGCATCGTGGTCGATAG
CCGGCTGGGCGAAGGGACGCGCTTTGACATGATCGTGCCGATGCGTGCGGTGTCGACGGCGGTGACGGAAGCCGAACTGC
CGCTGCCGCTTGGCCATGGGCAGCGCATTTTGCTGGTGGATGGCGAGGCCACGCGCCTATCGCTGCTGGGCAATGCGCTC
TCCAGCCAGGGCTATCAGCCGCAGCTGGCCAGCGATGGCGCCGCGGCGCTGCAACTGGTGCAGCAGCACGCGATGCCGGA
TCTGGTGATCATCGACAGCGACATCATCCTGCTCTCGGCAGTGAGCGTGCTGTTGAGCATGCAGGAGCTGGGGTACCAGG
GCCCGGCGATCGTGCTGGAAGATGTCGGTGCGCCGCTGCAGCGTGCGCATTTCCCGTCGGATCTGCCGGTGCATGTGCTG
CGCAAGCCGCTGGAAATGCGCCGGGTGTTCCGCGCCGTTTCGCATGCGCTGGAAGTGGCCTGA

Upstream 100 bases:

>100_bases
TGTGGTCAAGCCGGTGGATGTGGATCAGTTCTTCAACGCGGTCAAGACGCTCGGCACGTTCTGGGCGGTCATCAACCAGG
CACCGGAGTTGGACTGAACC

Downstream 100 bases:

>100_bases
GATCACGTTTGCGCGCAGCCGGCGCATGCGTCGACGGTGCAACGCGATTGCAACAGTTGACGTCCACGCCGGCCGCATGC
GCGAATAACGTATGACCACC

Product: histidine kinase-response regulator hybrid protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 500; Mature: 499

Protein sequence:

>500_residues
MPHEGGNLEQLKILLVEDSPEDAELLSDQLLEAGLDAAFERVDSEPSLRAALDDFQPDIVLSDLSMPGFSGHQALRLVRQ
NGATPFIFVSGTMGEETAVKALQDGANDYIIKHNPTRLPSAVIRAIREARADLERQRVESELMRAQRLESLAMLAAGLSH
DLRNILQPLLIVPDLLAGRTDDPQLRQLANVVAECGRRGHEMAESMLSFVRGSNKPREQVSIANLFQAVQMLLKSSLPDG
VRLQMDTIAADLTIEANYTELQQCLLNLGLNAIQAMPEGGRLILAADRYDASRVRMSVADTGVGMSDETRARLFSPFFTT
KADGTGLGLISCKRIIESYGGSIVVDSRLGEGTRFDMIVPMRAVSTAVTEAELPLPLGHGQRILLVDGEATRLSLLGNAL
SSQGYQPQLASDGAAALQLVQQHAMPDLVIIDSDIILLSAVSVLLSMQELGYQGPAIVLEDVGAPLQRAHFPSDLPVHVL
RKPLEMRRVFRAVSHALEVA

Sequences:

>Translated_500_residues
MPHEGGNLEQLKILLVEDSPEDAELLSDQLLEAGLDAAFERVDSEPSLRAALDDFQPDIVLSDLSMPGFSGHQALRLVRQ
NGATPFIFVSGTMGEETAVKALQDGANDYIIKHNPTRLPSAVIRAIREARADLERQRVESELMRAQRLESLAMLAAGLSH
DLRNILQPLLIVPDLLAGRTDDPQLRQLANVVAECGRRGHEMAESMLSFVRGSNKPREQVSIANLFQAVQMLLKSSLPDG
VRLQMDTIAADLTIEANYTELQQCLLNLGLNAIQAMPEGGRLILAADRYDASRVRMSVADTGVGMSDETRARLFSPFFTT
KADGTGLGLISCKRIIESYGGSIVVDSRLGEGTRFDMIVPMRAVSTAVTEAELPLPLGHGQRILLVDGEATRLSLLGNAL
SSQGYQPQLASDGAAALQLVQQHAMPDLVIIDSDIILLSAVSVLLSMQELGYQGPAIVLEDVGAPLQRAHFPSDLPVHVL
RKPLEMRRVFRAVSHALEVA
>Mature_499_residues
PHEGGNLEQLKILLVEDSPEDAELLSDQLLEAGLDAAFERVDSEPSLRAALDDFQPDIVLSDLSMPGFSGHQALRLVRQN
GATPFIFVSGTMGEETAVKALQDGANDYIIKHNPTRLPSAVIRAIREARADLERQRVESELMRAQRLESLAMLAAGLSHD
LRNILQPLLIVPDLLAGRTDDPQLRQLANVVAECGRRGHEMAESMLSFVRGSNKPREQVSIANLFQAVQMLLKSSLPDGV
RLQMDTIAADLTIEANYTELQQCLLNLGLNAIQAMPEGGRLILAADRYDASRVRMSVADTGVGMSDETRARLFSPFFTTK
ADGTGLGLISCKRIIESYGGSIVVDSRLGEGTRFDMIVPMRAVSTAVTEAELPLPLGHGQRILLVDGEATRLSLLGNALS
SQGYQPQLASDGAAALQLVQQHAMPDLVIIDSDIILLSAVSVLLSMQELGYQGPAIVLEDVGAPLQRAHFPSDLPVHVLR
KPLEMRRVFRAVSHALEVA

Specific function: Member of the two-component regulatory system zraS/zraR. May function as a membrane-associated protein kinase that phosphorylates zraR in response to high concentrations of zinc or lead in the medium [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=231, Percent_Identity=32.4675324675325, Blast_Score=118, Evalue=1e-27,
Organism=Escherichia coli, GI1790436, Length=249, Percent_Identity=32.9317269076305, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1790300, Length=267, Percent_Identity=29.5880149812734, Blast_Score=90, Evalue=4e-19,
Organism=Escherichia coli, GI1788713, Length=220, Percent_Identity=29.5454545454545, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI145693157, Length=378, Percent_Identity=26.1904761904762, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI48994928, Length=186, Percent_Identity=28.494623655914, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 54262; Mature: 54131

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPHEGGNLEQLKILLVEDSPEDAELLSDQLLEAGLDAAFERVDSEPSLRAALDDFQPDIV
CCCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCEE
LSDLSMPGFSGHQALRLVRQNGATPFIFVSGTMGEETAVKALQDGANDYIIKHNPTRLPS
EECCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHCCCCCEEEECCCCCCHH
AVIRAIREARADLERQRVESELMRAQRLESLAMLAAGLSHDLRNILQPLLIVPDLLAGRT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
DDPQLRQLANVVAECGRRGHEMAESMLSFVRGSNKPREQVSIANLFQAVQMLLKSSLPDG
CCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCC
VRLQMDTIAADLTIEANYTELQQCLLNLGLNAIQAMPEGGRLILAADRYDASRVRMSVAD
CEEEEEHEEEEEEEECCHHHHHHHHHHHCCHHHHCCCCCCEEEEEECCCCHHHHEEEHHH
TGVGMSDETRARLFSPFFTTKADGTGLGLISCKRIIESYGGSIVVDSRLGEGTRFDMIVP
CCCCCCCCHHHHHHCCHHCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCEEEEEC
MRAVSTAVTEAELPLPLGHGQRILLVDGEATRLSLLGNALSSQGYQPQLASDGAAALQLV
HHHHHHHHHHCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
QQHAMPDLVIIDSDIILLSAVSVLLSMQELGYQGPAIVLEDVGAPLQRAHFPSDLPVHVL
HHHCCCCEEEECCCHHHHHHHHHHHHHHHCCCCCCEEEEHHCCCCHHHHCCCCCCCHHHH
RKPLEMRRVFRAVSHALEVA
HCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PHEGGNLEQLKILLVEDSPEDAELLSDQLLEAGLDAAFERVDSEPSLRAALDDFQPDIV
CCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCEE
LSDLSMPGFSGHQALRLVRQNGATPFIFVSGTMGEETAVKALQDGANDYIIKHNPTRLPS
EECCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHCCCCCEEEECCCCCCHH
AVIRAIREARADLERQRVESELMRAQRLESLAMLAAGLSHDLRNILQPLLIVPDLLAGRT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
DDPQLRQLANVVAECGRRGHEMAESMLSFVRGSNKPREQVSIANLFQAVQMLLKSSLPDG
CCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCC
VRLQMDTIAADLTIEANYTELQQCLLNLGLNAIQAMPEGGRLILAADRYDASRVRMSVAD
CEEEEEHEEEEEEEECCHHHHHHHHHHHCCHHHHCCCCCCEEEEEECCCCHHHHEEEHHH
TGVGMSDETRARLFSPFFTTKADGTGLGLISCKRIIESYGGSIVVDSRLGEGTRFDMIVP
CCCCCCCCHHHHHHCCHHCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCEEEEEC
MRAVSTAVTEAELPLPLGHGQRILLVDGEATRLSLLGNALSSQGYQPQLASDGAAALQLV
HHHHHHHHHHCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
QQHAMPDLVIIDSDIILLSAVSVLLSMQELGYQGPAIVLEDVGAPLQRAHFPSDLPVHVL
HHHCCCCEEEECCCHHHHHHHHHHHHHHHCCCCCCEEEEHHCCCCHHHHCCCCCCCHHHH
RKPLEMRRVFRAVSHALEVA
HCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]