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The map label for this gene is dapF

Identifier: 21241405

GI number: 21241405

Start: 749074

End: 749886

Strand: Direct

Name: dapF

Synonym: XAC0634

Alternate gene names: 21241405

Gene position: 749074-749886 (Clockwise)

Preceding gene: 21241404

Following gene: 21241406

Centisome position: 14.47

GC content: 68.39

Gene sequence:

>813_bases
ATGCATGGCGCCGGCAACGATTTCGTGGTGCTGGACCTGCGCGACGGTACGCCACCACCGGATGCCGCACTGGCAGCGCA
GTTGGCCGACCGCCACTTCGGAATTGGCTGTGATCAGATCCTGACCATCGAGGCGCCGCGCAGCGAAGGCGCCGCGGCAG
CCTACGGCATCTGGAATTCCGATGGCTCGGCCGCGCGCCAATGCGGCAATGGCGCACGTTGTGTCGCCGCGTGGCTGGTG
CGCGACGGCACCGCCCAGGGGGAGCGCTTCATCATCGACAGTCCGGCGACCGCGCACGCGGTCGAGCGCCTCGACGGCGA
CCGCTACGCGGTGGCGATGGGCGTCCCGCAGTTCGAGCCCACCCAGATTCCGCTGGCCGGGTTTGCGCATGCACGCGACG
AATATGCGCTGCCGGTGCACGGCGAGACCGTGCGCTTTGGTGCGGTGTCGATGGGCAACCCGCACGCGGTGGTCGAGGTC
GGTCGCGTCGATGCAGCGCCGGTGGAACGCGTGGGCGCGCTGCTGCAGCAAAACGCTGCGTTTCCCGATTCGGTCAATGT
GGGTTTCGCGCAGGTGGTCGACCCCGTGCATGTGCGCCTGCGCGTGTTCGAGCGCGGCGTTGGCGAAACGCTGGCCTGCG
GAAGTGGCGCCTGTGCGGCTGCGGCGGTGTTGATGCAGCGCGGCCGTGTCGAGCGCGATGTGCAGGTGTCCTTGCCCGGT
GGCGAGTTACGCATTCGTTGGCCCGGCGACCAGGAGCAGGTGGTGATGTCCGGCCCGGCTGTGTTCGTCTTCGATGGAGA
GTGGAACCGATGA

Upstream 100 bases:

>100_bases
CCGGTGGATGGCGACACCAAGCCTGCATCCACCACCGACCAGCCCGCTAGCAGCGGCAATGAGCGCTGACGGTCGCAGCG
GGCGTCTGCGTTTCACCAAA

Downstream 100 bases:

>100_bases
GCGAGAACACGGATAAATTCGGCGCGCACGAGGTGGCCACGTGGTTGCGACGTCACCCCACCTTCCTCAAGCAGTTCCCG
GATCTGGCGGTGAGCTTGCT

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase

Number of amino acids: Translated: 270; Mature: 270

Protein sequence:

>270_residues
MHGAGNDFVVLDLRDGTPPPDAALAAQLADRHFGIGCDQILTIEAPRSEGAAAAYGIWNSDGSAARQCGNGARCVAAWLV
RDGTAQGERFIIDSPATAHAVERLDGDRYAVAMGVPQFEPTQIPLAGFAHARDEYALPVHGETVRFGAVSMGNPHAVVEV
GRVDAAPVERVGALLQQNAAFPDSVNVGFAQVVDPVHVRLRVFERGVGETLACGSGACAAAAVLMQRGRVERDVQVSLPG
GELRIRWPGDQEQVVMSGPAVFVFDGEWNR

Sequences:

>Translated_270_residues
MHGAGNDFVVLDLRDGTPPPDAALAAQLADRHFGIGCDQILTIEAPRSEGAAAAYGIWNSDGSAARQCGNGARCVAAWLV
RDGTAQGERFIIDSPATAHAVERLDGDRYAVAMGVPQFEPTQIPLAGFAHARDEYALPVHGETVRFGAVSMGNPHAVVEV
GRVDAAPVERVGALLQQNAAFPDSVNVGFAQVVDPVHVRLRVFERGVGETLACGSGACAAAAVLMQRGRVERDVQVSLPG
GELRIRWPGDQEQVVMSGPAVFVFDGEWNR
>Mature_270_residues
MHGAGNDFVVLDLRDGTPPPDAALAAQLADRHFGIGCDQILTIEAPRSEGAAAAYGIWNSDGSAARQCGNGARCVAAWLV
RDGTAQGERFIIDSPATAHAVERLDGDRYAVAMGVPQFEPTQIPLAGFAHARDEYALPVHGETVRFGAVSMGNPHAVVEV
GRVDAAPVERVGALLQQNAAFPDSVNVGFAQVVDPVHVRLRVFERGVGETLACGSGACAAAAVLMQRGRVERDVQVSLPG
GELRIRWPGDQEQVVMSGPAVFVFDGEWNR

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family

Homologues:

Organism=Escherichia coli, GI87082334, Length=266, Percent_Identity=44.3609022556391, Blast_Score=220, Evalue=6e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPF_XANAC (Q8PPQ1)

Other databases:

- EMBL:   AE008923
- RefSeq:   NP_640987.1
- ProteinModelPortal:   Q8PPQ1
- SMR:   Q8PPQ1
- GeneID:   1154705
- GenomeReviews:   AE008923_GR
- KEGG:   xac:XAC0634
- NMPDR:   fig|190486.1.peg.631
- HOGENOM:   HBG399442
- OMA:   HTGIGFD
- ProtClustDB:   PRK00450
- BioCyc:   XAXO190486:XAC0634-MONOMER
- BRENDA:   5.1.1.7
- GO:   GO:0005737
- HAMAP:   MF_00197
- InterPro:   IPR001653
- InterPro:   IPR018510
- TIGRFAMs:   TIGR00652

Pfam domain/function: PF01678 DAP_epimerase

EC number: =5.1.1.7

Molecular weight: Translated: 28528; Mature: 28528

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: ACT_SITE 82-82 ACT_SITE 227-227

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHGAGNDFVVLDLRDGTPPPDAALAAQLADRHFGIGCDQILTIEAPRSEGAAAAYGIWNS
CCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCEEEEEEECC
DGSAARQCGNGARCVAAWLVRDGTAQGERFIIDSPATAHAVERLDGDRYAVAMGVPQFEP
CCHHHHHCCCCCCEEEEEEHHCCCCCCCEEEEECCCHHHHHHHCCCCEEEEEECCCCCCC
TQIPLAGFAHARDEYALPVHGETVRFGAVSMGNPHAVVEVGRVDAAPVERVGALLQQNAA
CCCCHHHHCCCCCCEECCCCCCEEEEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHCCC
FPDSVNVGFAQVVDPVHVRLRVFERGVGETLACGSGACAAAAVLMQRGRVERDVQVSLPG
CCCCCCCCHHHHHHHHHHHHHEECCCCCCEECCCCCHHHHHHHHHHCCCCCCCEEEECCC
GELRIRWPGDQEQVVMSGPAVFVFDGEWNR
CEEEEECCCCCCEEEEECCEEEEECCCCCC
>Mature Secondary Structure
MHGAGNDFVVLDLRDGTPPPDAALAAQLADRHFGIGCDQILTIEAPRSEGAAAAYGIWNS
CCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCEEEEEEECC
DGSAARQCGNGARCVAAWLVRDGTAQGERFIIDSPATAHAVERLDGDRYAVAMGVPQFEP
CCHHHHHCCCCCCEEEEEEHHCCCCCCCEEEEECCCHHHHHHHCCCCEEEEEECCCCCCC
TQIPLAGFAHARDEYALPVHGETVRFGAVSMGNPHAVVEVGRVDAAPVERVGALLQQNAA
CCCCHHHHCCCCCCEECCCCCCEEEEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHCCC
FPDSVNVGFAQVVDPVHVRLRVFERGVGETLACGSGACAAAAVLMQRGRVERDVQVSLPG
CCCCCCCCHHHHHHHHHHHHHEECCCCCCEECCCCCHHHHHHHHHHCCCCCCCEEEECCC
GELRIRWPGDQEQVVMSGPAVFVFDGEWNR
CEEEEECCCCCCEEEEECCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217