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The map label for this gene is purD

Identifier: 21241282

GI number: 21241282

Start: 600921

End: 602216

Strand: Reverse

Name: purD

Synonym: XAC0511

Alternate gene names: 21241282

Gene position: 602216-600921 (Counterclockwise)

Preceding gene: 21241283

Following gene: 21241281

Centisome position: 11.64

GC content: 69.14

Gene sequence:

>1296_bases
TTGAAAATCCTCGTCATCGGCTCCGGCGGCCGTGAACATGCCCTGGCCTGGAAGATCGCGCAGTCCGCGCGCGTGAGCGA
GGTGCTGGTGGCACCGGGCAATGCCGGCACCGCCACCGAAGCCAAGTGCCGCAACGTCGCGATCAAGGTCGACGACCTGG
ATGGCCTGCTCGCCCTGGCGCAGCGCGAAGCCGTCGCGCTCACCGTGGTCGGCCCGGAAGTGCCGCTGGTGCTGGGCGTG
GTCGACCGCTTCCATGCCGCCGGCCTGCGCATCTTCGGCCCCACCGCCAAGGCCGCGCAGCTGGAAGGCAGCAAGGCCTT
CGCCAAGGATTTCCTCGCCCGCCACGGCATTCCCACCGCGTATTACGCCGTGCACACCGAAGTGGACGCCGCGCTGGCCT
ACGTGCGCGAGAAAGGCACGCCCATCGTGGTCAAGGCCGACGGCCTGGCTGCCGGCAAGGGCGTGATCGTGGCGCTGACC
CTGGCCGAGGCCGAAGATGCGGTGCGCGACATGCTCTCTGGCAATGCGTTCGGCGATGCCGGCGCACGCGTGGTCATCGA
GGAATTCCTCGATGGGGAAGAGGCCAGCTTCATCTCGATGGTCGATGGCAAACACGCCTTGCCGATGGCCACCAGCCAGG
ACCACAAGCGTGTCGGCGACGGCGACACCGGCCCGAATACCGGCGGCATGGGCGCCTACTCACCTGCGCCGGTGGTCACG
CCCGAGGTGCATGCGCGGGTGATGCGCGAAGTGGTCGAGCCGACCGTGCAGGGCATGATCGCCGATGGCGTGCCGTTCAC
CGGTTTTCTGTATGCCGGGCTGATGATCGACGCGCATGGCGCGCCGAAGGTGATCGAATTCAACGTGCGCTTCGGCGACC
CGGAAACCCAGCCGGTGATGCTGCGCCTGCAGTCGGATCTGGTCGATCTGCTCGAGGCGGCCATCGACGGCAAGCTCGAC
GGCGCGCATGCGCAGTGGGATCCGCGCCCGTCGCTTGGCGTGGTCATCGCCGCCAGGCCTTACCCCGACACCCCGGTCAC
CGGTGAGGTCATCGCCGGGCTGGACGCGGTGCCGGCCAGCGCCAAGGTCTTCCATGCCGGCACCGCGTTGAATGCCGCTG
GCGAAGTGGTCAGCGCCGGTGGCCGCGTGCTGTGCGTCGCCGCCCTGGGCGACAGCGTGCAGGACGCCCAGCGCACCGCC
TATGCCGGCCTCAAGCCGATCCACTGGAGCAGCGCCTTCCAGCGCAGCGACATCGGCTGGCGGGCGATCGCCCGCGAGCG
GCAGGCGCAGGGCTAA

Upstream 100 bases:

>100_bases
CGCGCTCGACACCAAGACCTGGACCTACCCGTTTCCCAACGCACTGCAGCGCGAAGGCTGCAGATAGCGCCCCCTTCCCC
CTTACTAGCGAGCAACTGCC

Downstream 100 bases:

>100_bases
GCCGCGTCCTTCCCCCGCCGGCGGGGAAGGTGCCCGAAGGGCGGATGGGGGCAAGTTCGGACATCCACCGTATCGGCCGA
ACAGAGCAACAGCGCGCTGG

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MKILVIGSGGREHALAWKIAQSARVSEVLVAPGNAGTATEAKCRNVAIKVDDLDGLLALAQREAVALTVVGPEVPLVLGV
VDRFHAAGLRIFGPTAKAAQLEGSKAFAKDFLARHGIPTAYYAVHTEVDAALAYVREKGTPIVVKADGLAAGKGVIVALT
LAEAEDAVRDMLSGNAFGDAGARVVIEEFLDGEEASFISMVDGKHALPMATSQDHKRVGDGDTGPNTGGMGAYSPAPVVT
PEVHARVMREVVEPTVQGMIADGVPFTGFLYAGLMIDAHGAPKVIEFNVRFGDPETQPVMLRLQSDLVDLLEAAIDGKLD
GAHAQWDPRPSLGVVIAARPYPDTPVTGEVIAGLDAVPASAKVFHAGTALNAAGEVVSAGGRVLCVAALGDSVQDAQRTA
YAGLKPIHWSSAFQRSDIGWRAIARERQAQG

Sequences:

>Translated_431_residues
MKILVIGSGGREHALAWKIAQSARVSEVLVAPGNAGTATEAKCRNVAIKVDDLDGLLALAQREAVALTVVGPEVPLVLGV
VDRFHAAGLRIFGPTAKAAQLEGSKAFAKDFLARHGIPTAYYAVHTEVDAALAYVREKGTPIVVKADGLAAGKGVIVALT
LAEAEDAVRDMLSGNAFGDAGARVVIEEFLDGEEASFISMVDGKHALPMATSQDHKRVGDGDTGPNTGGMGAYSPAPVVT
PEVHARVMREVVEPTVQGMIADGVPFTGFLYAGLMIDAHGAPKVIEFNVRFGDPETQPVMLRLQSDLVDLLEAAIDGKLD
GAHAQWDPRPSLGVVIAARPYPDTPVTGEVIAGLDAVPASAKVFHAGTALNAAGEVVSAGGRVLCVAALGDSVQDAQRTA
YAGLKPIHWSSAFQRSDIGWRAIARERQAQG
>Mature_431_residues
MKILVIGSGGREHALAWKIAQSARVSEVLVAPGNAGTATEAKCRNVAIKVDDLDGLLALAQREAVALTVVGPEVPLVLGV
VDRFHAAGLRIFGPTAKAAQLEGSKAFAKDFLARHGIPTAYYAVHTEVDAALAYVREKGTPIVVKADGLAAGKGVIVALT
LAEAEDAVRDMLSGNAFGDAGARVVIEEFLDGEEASFISMVDGKHALPMATSQDHKRVGDGDTGPNTGGMGAYSPAPVVT
PEVHARVMREVVEPTVQGMIADGVPFTGFLYAGLMIDAHGAPKVIEFNVRFGDPETQPVMLRLQSDLVDLLEAAIDGKLD
GAHAQWDPRPSLGVVIAARPYPDTPVTGEVIAGLDAVPASAKVFHAGTALNAAGEVVSAGGRVLCVAALGDSVQDAQRTA
YAGLKPIHWSSAFQRSDIGWRAIARERQAQG

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Homo sapiens, GI28416899, Length=430, Percent_Identity=48.6046511627907, Blast_Score=392, Evalue=1e-109,
Organism=Homo sapiens, GI4503915, Length=430, Percent_Identity=48.6046511627907, Blast_Score=391, Evalue=1e-109,
Organism=Homo sapiens, GI209869995, Length=430, Percent_Identity=48.6046511627907, Blast_Score=391, Evalue=1e-109,
Organism=Homo sapiens, GI209869993, Length=430, Percent_Identity=48.6046511627907, Blast_Score=391, Evalue=1e-109,
Organism=Escherichia coli, GI1790438, Length=429, Percent_Identity=69.4638694638695, Blast_Score=583, Evalue=1e-168,
Organism=Caenorhabditis elegans, GI17567511, Length=425, Percent_Identity=40.7058823529412, Blast_Score=298, Evalue=3e-81,
Organism=Saccharomyces cerevisiae, GI6321203, Length=446, Percent_Identity=41.2556053811659, Blast_Score=344, Evalue=2e-95,
Organism=Drosophila melanogaster, GI62471619, Length=430, Percent_Identity=45.8139534883721, Blast_Score=345, Evalue=3e-95,
Organism=Drosophila melanogaster, GI24582400, Length=430, Percent_Identity=45.8139534883721, Blast_Score=344, Evalue=8e-95,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PUR2_XANAC (Q8PQ21)

Other databases:

- EMBL:   AE008923
- RefSeq:   NP_640864.1
- ProteinModelPortal:   Q8PQ21
- SMR:   Q8PQ21
- GeneID:   1154582
- GenomeReviews:   AE008923_GR
- KEGG:   xac:XAC0511
- NMPDR:   fig|190486.1.peg.508
- HOGENOM:   HBG404060
- OMA:   GVPIVIK
- ProtClustDB:   PRK00885
- BioCyc:   XAXO190486:XAC0511-MONOMER
- BRENDA:   6.3.4.13
- HAMAP:   MF_00138
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.90.600.10
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR00877

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N; SSF52440 PreATP-grasp-like; SSF51246 Rudmnt_hyb_motif

EC number: =6.3.4.13

Molecular weight: Translated: 44891; Mature: 44891

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: PS50975 ATP_GRASP; PS00184 GARS; PS00583 PFKB_KINASES_1 ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILVIGSGGREHALAWKIAQSARVSEVLVAPGNAGTATEAKCRNVAIKVDDLDGLLALA
CEEEEEECCCCCCHHEEEHHHCCCCEEEEEECCCCCCCCCCCCCEEEEEEECCCHHHHHH
QREAVALTVVGPEVPLVLGVVDRFHAAGLRIFGPTAKAAQLEGSKAFAKDFLARHGIPTA
CCCEEEEEEECCCCCHHHHHHHHHHHCCEEEECCCCCHHHCCCCHHHHHHHHHHCCCCEE
YYAVHTEVDAALAYVREKGTPIVVKADGLAAGKGVIVALTLAEAEDAVRDMLSGNAFGDA
EEEEEHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEEEEHHHHHHHHHHHCCCCCCCC
GARVVIEEFLDGEEASFISMVDGKHALPMATSQDHKRVGDGDTGPNTGGMGAYSPAPVVT
CHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PEVHARVMREVVEPTVQGMIADGVPFTGFLYAGLMIDAHGAPKVIEFNVRFGDPETQPVM
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCEEEECCCCCEEEEEEEEECCCCCCCEE
LRLQSDLVDLLEAAIDGKLDGAHAQWDPRPSLGVVIAARPYPDTPVTGEVIAGLDAVPAS
EEEHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHCCCCCCCC
AKVFHAGTALNAAGEVVSAGGRVLCVAALGDSVQDAQRTAYAGLKPIHWSSAFQRSDIGW
CEEEECCCHHCCCCHHHHCCCCEEEEEECCCCHHHHHHHHHCCCCCEECCCHHHCCCCCH
RAIARERQAQG
HHHHHHHHCCC
>Mature Secondary Structure
MKILVIGSGGREHALAWKIAQSARVSEVLVAPGNAGTATEAKCRNVAIKVDDLDGLLALA
CEEEEEECCCCCCHHEEEHHHCCCCEEEEEECCCCCCCCCCCCCEEEEEEECCCHHHHHH
QREAVALTVVGPEVPLVLGVVDRFHAAGLRIFGPTAKAAQLEGSKAFAKDFLARHGIPTA
CCCEEEEEEECCCCCHHHHHHHHHHHCCEEEECCCCCHHHCCCCHHHHHHHHHHCCCCEE
YYAVHTEVDAALAYVREKGTPIVVKADGLAAGKGVIVALTLAEAEDAVRDMLSGNAFGDA
EEEEEHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEEEEHHHHHHHHHHHCCCCCCCC
GARVVIEEFLDGEEASFISMVDGKHALPMATSQDHKRVGDGDTGPNTGGMGAYSPAPVVT
CHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PEVHARVMREVVEPTVQGMIADGVPFTGFLYAGLMIDAHGAPKVIEFNVRFGDPETQPVM
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCEEEECCCCCEEEEEEEEECCCCCCCEE
LRLQSDLVDLLEAAIDGKLDGAHAQWDPRPSLGVVIAARPYPDTPVTGEVIAGLDAVPAS
EEEHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHCCCCCCCC
AKVFHAGTALNAAGEVVSAGGRVLCVAALGDSVQDAQRTAYAGLKPIHWSSAFQRSDIGW
CEEEECCCHHCCCCHHHHCCCCEEEEEECCCCHHHHHHHHHCCCCCEECCCHHHCCCCCH
RAIARERQAQG
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12024217